Cyclopropane fatty acids are involved in organic solvent tolerance but not in acid stress resistance in Pseudomonas putida DOT‐T1E
Open accessAbstract
Summary: Bacterial membranes constitute the first physical barrier against different environmental stresses. Pseudomonas putida DOT‐T1E accumulates cyclopropane fatty acids (CFAs) in the stationary phase of growth. In this strain the cfaB gene encodes the main cyclopropane synthase responsible of the synthesis of CFAs, and its expression is mediated by RNA polymerase with sigma factor σ 38 . We generated a cfaB mutant of P. putida DOT‐T1E and studied its response to solvents, acid pH and other stress conditions such as temperature changes, high osmolarity and the presence of antibiotics or heavy metals in the culture medium. A CfaB knockout mutant was more sensitive to solvent stress than the wild‐type strain, but in contrast to Escherichia coli and Salmonella enterica , the P. putida cfaB mutant was as tolerant to acid shock as the wild‐type strain. The cfaB mutant was also as tolerant as the parental strain to a number of drugs, antibiotics and other damaging agents.
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Elevated production of cyclopropyl fatty acids was found in Pseudomonas as a membrane adaptation that increases tolerance to organic solvents and polycyclic aromatic hydrocarbons.
“For example, elevated production of CPFAs was found in Pseudomonas as a membrane adaptation that increases tolerance to organic solvents and polycyclic aromatic hydrocarbons, possibly hindering accumulation of the pollutants in the cellular membrane (Pini et al., 2009).”
From A stable isotope assay with 13C-labeled polyethylene to investigate plastic mineralization mediated by Rhodococcus ruber (2023) · cites this paper as Pini et al., 2009
The Results report that loss of cyclopropane fatty acids reduced Pseudomonas putida DOT-T1E survival after organic-solvent exposure, supporting their role in solvent tolerance. But the Discussion says CFA content did not increase after toluene was added. The paper does not establish tolerance to polycyclic aromatic hydrocarbons or show that CFAs hinder pollutant accumulation in the membrane. Page numbers were not available in our copy. (LLM, conf=0.89)
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- Authors
- Pini, C., Bernal, P., Godoy, P., Ramos, J., & Segura, A.
- Affiliations
- Consejo Superior de Investigaciones Científicas (ES)
- University of London (GB)
- Corresponding author
- Segura, Ana
- Venue
- Microbial Biotechnology
- Publisher
- Wiley
- Published
- 2009-02-18
- Volume
- 2
- Issue
- 2
- Pages
- 253-261
- ISSN
- 1751-7915 (ISSN-L)
- PMC
- PMC3815845
- OpenAlex
- W2075937910
- Cited by
- 60
- References
- 49
- Citations by year
- 2026: 3 · 2025: 2 · 2024: 1 · 2023: 3 · 2022: 4 · 2021: 5 · 2020: 3 · 2019: 7 · 2018: 1 · 2017: 3 · 2016: 3 · 2015: 6 · 2014: 2 · 2013: 4 · 2012: 5
- FWCI
- 2.53
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Microbial Biotechnology (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Spiral (Imperial College London) (submittedVersion, cc-by)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
- Keywords
- cyclopropane fatty acids, Pseudomonas putida DOT-T1E, organic solvent tolerance, acid resistance, stationary phase, antibiotic tolerance, heavy metal tolerance, osmotic stress, temperature stress, knockout mutants
- MeSH terms
- Acids, Bacterial Proteins, Cyclopropanes, Fatty Acids, Methyltransferases, Molecular Sequence Data, Organic Chemicals, Stress, Physiological, Pseudomonas putida
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