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Nitric oxide‐mediated dispersal in single‐ and multi‐species biofilms of clinically and industrially relevant microorganisms
Open accessAbstract
Summary: Strategies to induce biofilm dispersal are of interest due to their potential to prevent biofilm formation and biofilm‐related infections. Nitric oxide (NO), an important messenger molecule in biological systems, was previously identified as a signal for dispersal in biofilms of the model organism Pseudomonas aeruginosa . In the present study, the use of NO as an anti‐biofilm agent more broadly was assessed. Various NO donors, at concentrations estimated to generate NO levels in the picomolar and low nanomolar range, were tested on single‐species biofilms of relevant microorganisms and on multi‐species biofilms from water distribution and treatment systems. Nitric oxide‐induced dispersal was observed in all biofilms assessed, and the average reduction of total biofilm surface was 63%. Moreover, biofilms exposed to low doses of NO were more susceptible to antimicrobial treatments than untreated biofilms. For example, the efficacy of conventional chlorine treatments at removing multi‐species biofilms from water systems was increased by 20‐fold in biofilms treated with NO compared with untreated biofilms. These data suggest that combined treatments with NO may allow for novel and improved strategies to control biofilms and have widespread applications in many environmental, industrial and clinical settings.
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Metadata
- Authors
- Barraud, N., Storey, M. V., Moore, Z. P., Webb, J. S., Rice, S. A., & Kjelleberg, S.
- Affiliations
- UNSW Sydney (AU)
- Corresponding author
- Kjelleberg, Staffan
- Venue
- Microbial Biotechnology
- Publisher
- Wiley
- Published
- 2009-04-17
- Volume
- 2
- Issue
- 3
- Pages
- 370-378
- ISSN
- 1751-7915 (ISSN-L)
- PMC
- PMC3815757
- OpenAlex
- W2040417898
- Cited by
- 289
- References
- 40
- Citations by year
- 2026: 7 · 2025: 5 · 2024: 11 · 2023: 18 · 2022: 20 · 2021: 21 · 2020: 20 · 2019: 25 · 2018: 28 · 2017: 29 · 2016: 27 · 2015: 23 · 2014: 15 · 2013: 14 · 2012: 16
- FWCI
- 3.49
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Microbial Biotechnology (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- ePrints Soton (University of Southampton) (submittedVersion)
- Funders
- Biotechnology and Biological Sciences Research Council (BB/C520655/1)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bacterial biofilms and quorum sensing
- Physical Sciences › Environmental Science › Pollution › Wastewater Treatment and Nitrogen Removal
- Physical Sciences › Engineering › Ocean Engineering › Marine Biology and Environmental Chemistry
- Keywords
- nitric oxide, biofilm dispersal, nitric oxide donors, polymicrobial biofilms, antibiofilm agents, biofilm control, chlorine disinfection, water distribution systems, water treatment, antimicrobial susceptibility, biofilm-associated infections, Pseudomonas aeruginosa
- MeSH terms
- Bacteria, Humans, Mycoses, Nitric Oxide, Pseudomonas Infections, Yeasts, Bacterial Physiological Phenomena, Biofilms, Nitric Oxide Donors
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