Molecular investigation of the viral kinetics of equine herpesvirus-1 in blood and nasal secretions of horses after corticosteroid-induced recrudescence of latent infection.
Open accessAbstract
BACKGROUND: Recrudescence of latent equine herpesvirus 1 (EHV-1) with subsequent viral shedding via nasal secretions is a potential source of infection for susceptible horses and has been implicated in outbreaks occurring in closed populations. OBJECTIVES: To describe the viral kinetics of reactivated EHV-1 in blood and nasal secretions from latently infected horses after administration of corticosteroids, and to study the infectious nature of reactivated EHV-1 to sentinel horses. ANIMALS: Eight healthy horses. METHODS: Four horses infected 4 months previously with EHV-1 received dexamethasone on 5 consecutive days. Four seronegative horses served as sentinels and had direct contact with the latently infected horses. All horses were monitored daily for development of clinical signs. Whole blood and nasal secretions were collected daily for molecular detection and cell culture of EHV-1. Serum was collected weekly for the detection of antibodies against EHV-1. RESULTS: All horses in the latently infected group showed transient molecular detection of EHV-1 in blood and nasal secretions, but only 1 horse developed fever. Three latently infected horses developed an increase in antibody concentrations against EHV-l. Viral cultures remained negative for all latently infected horses after corticosteroid administration. None of the sentinel horses developed clinical signs, viremia, viral shedding, or seroconversion. CONCLUSIONS AND CLINICAL IMPORTANCE: EHV-1 was successfully reactivated after corticosteroid administration in latently infected horses. However, transmission of reactivated virus to sentinel horses was unsuccessful. Failure to effectively transmit EHV-1 to susceptible horses may have resulted from the low level and short period of viral shedding in latently infected horses.
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Cites
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Research works printed but not linked: 1
- Pusterla N, Mapes S. Detection and characterization of equine herpesvirus-1 strains in submandibular lymph nodes and trigeminal ganglia from horses using real-time PCR. Proceedings, Annual ACVIM Forum, San Antonio, TX, 2008: 818.
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Laval, K., Poelaert, K. C. K., Van Cleemput, J., Zhao, J., Vandekerckhove, A. P., Gryspeerdt, A. C., Garré, B., van der Meulen, K., Baghi, H. B., Dubale, H. N., et al. (2021) · Frontiers in Microbiology · doi:10.3389/fmicb.2021.662686 · Cited by 54 · see citing works
Spiesschaert, B., Goldenbogen, B., Taferner, S., Schade, M., Mahmoud, M., Klipp, E., Osterrieder, N., & Azab, W. (2015) · Journal of Virology · doi:10.1128/jvi.01809-15 · Cited by 24 · see citing works
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- Authors
- Pusterla, N., Hussey, S. B., Mapes, S., Johnson, C., Collier, J. R., Hill, J., Lunn, D. P., & Wilson, W. D.
- Affiliations
- University of California, Davis (US)
- Colorado State University (US)
- Corresponding author
- Pusterla, N.
- Venue
- Journal of Veterinary Internal Medicine
- Publisher
- Oxford University Press (OUP)
- Published
- 2010-09-01
- Volume
- 24
- Issue
- 5
- Pages
- 1153-1157
- ISSN
- 0891-6640 (ISSN-L)
- 1939-1676
- OpenAlex
- W2096028163
- Cited by
- 38
- References
- 16
- Citations by year
- 2026: 2 · 2025: 2 · 2024: 2 · 2023: 1 · 2022: 5 · 2021: 3 · 2020: 2 · 2019: 1 · 2018: 2 · 2017: 4 · 2015: 5 · 2014: 5 · 2013: 2 · 2012: 2
- FWCI
- 0.93
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model (vor)
- Locations
- Journal of Veterinary Internal Medicine (publishedVersion)
- PubMed (publishedVersion)
- Funders
- School of Veterinary Medicine, University of California, Davis
- University of California, Davis
- Topics
- Health Sciences › Medicine › Epidemiology › Herpesvirus Infections and Treatments
- Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Animal Virus Infections Studies
- Health Sciences › Veterinary › Equine › Veterinary Equine Medical Research
- Keywords
- equine herpesvirus 1, viral reactivation, latent infection, corticosteroids, dexamethasone, viral shedding, nasal secretions, viremia, virus transmission, antibody response, horses
- MeSH terms
- Adrenal Cortex Hormones, Animals, Dexamethasone, Herpesvirus 1, Equid, Horse Diseases, Horses, Male, Mucus, Time Factors, Virus Replication, Virus Latency
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