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Cited by 154Mesenchymal stem cell researchHematopoietic Stem Cell TransplantationPluripotent Stem Cells Research

CD 146 expression on mesenchymal stem cells is associated with their vascular smooth muscle commitment

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Espagnolle, Nicolas, Guilloton, Fabien, Deschaseaux, Frédéric, Gadelorge, Mélanie, Sensébé, Luc, Bourin, Philippe·2014·Journal of Cellular and Molecular Medicine
DOI 10.1111/jcmm.12168PMC PMC3916122
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Abstract

Bone marrow mesenchymal stem cells (MSC s) are plastic adherent cells that can differentiate into various tissue lineages, including osteoblasts, adipocytes and chondrocytes. However, this progenitor property is not shared by all cells within the MSC population. In addition, MSC s vary in their proliferation capacity and expression of markers. Because of heterogeneity of CD 146 expression in the MSC population, we compared CD 146−/Low and CD 146 High cells under clonal conditions and after sorting of the non‐clonal cell population to determine whether this expression is associated with specific functions. CD 146−/Low and CD 146 High bone marrow MSC s did not differ in colony‐forming unit‐fibroblast number, osteogenic, adipogenic and chondrogenic differentiation or in vitro haematopoietic‐supportive activity. However, CD 146−/Low clones proliferated slightly but significantly faster than did CD 146 High clones. In addition, a strong expression of CD 146 molecule was associated with a commitment to a vascular smooth muscle cell (VSMC) lineage characterized by a strong up‐regulation of calponin‐1 and SM 22α expression and an ability to contract collagen matrix. Thus, within a bone marrow MSC population, certain subpopulations characterized by high expression of CD 146, are committed towards a VSMC lineage.

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  • CD146 expression in MSCs plays a key role in the perivascular niche, skeletogenesis, and hematopoietic support in vivo.

    “CD146 expression in MSCs plays a key role in the perivascular niche, skeletogenesis, and hematopoietic support in vivo [26,27,28].”

    From Mesenchymal Stem/Stromal Cells: Immunomodulatory and Bone Regeneration Potential after Tumor Excision in Osteosarcoma Patients (2023) · cites this paper as [28]

    The Introduction reports that CD146-expressing marrow cells occur in perivascular regions and that CD146+ cells were osteogenic in vivo and re-established a haematopoietic microenvironment in a cited xenotransplantation study. But this paper treats CD146 chiefly as a marker associated with these properties, not as a demonstrated causal factor that plays a key role in all three; its own comparisons found no difference in osteogenic differentiation or in vitro haematopoietic support between CD146-high and CD146-low MSCs (Abstract; Discussion). (LLM, conf=0.84)

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Authors
Espagnolle, N., Guilloton, F., Deschaseaux, F., Gadelorge, M., Sensébé, L., & Bourin, P.
Affiliations
  • Centre National de la Recherche Scientifique (FR)
  • EFS Pyrénées Méditerranée UMR5273 CNRS/UPS/EFS Inserm U1031 STROMALab Toulouse France
Corresponding author
Espagnolle, Nicolas
ORCID
Venue
Journal of Cellular and Molecular Medicine
Publisher
Wiley
Published
2013-11-04
Volume
18
Issue
1
Pages
104-114
ISSN
  • 1582-1838 (ISSN-L)
  • 1582-4934
DOI
10.1111/jcmm.12168
PMC
PMC3916122
OpenAlex
W2047802007
Cited by
154
References
2
Citations by year
2026: 5 · 2025: 9 · 2024: 7 · 2023: 7 · 2022: 7 · 2021: 21 · 2020: 23 · 2019: 13 · 2018: 12 · 2017: 12 · 2016: 18 · 2015: 16 · 2014: 3 · 2013: 1
FWCI
6.24
Type
journal article
Language
en
Open access
Licence
Locations
  • Journal of Cellular and Molecular Medicine (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
Funders
  • Institut National de la Santé et de la Recherche Médicale (UMR1043)
  • Centre National de la Recherche Scientifique
  • Institut National Du Cancer
Topics
  • Health Sciences › Medicine › Genetics › Mesenchymal stem cell research
  • Health Sciences › Medicine › Hematology › Hematopoietic Stem Cell Transplantation
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pluripotent Stem Cells Research
Keywords
CD146, bone marrow mesenchymal stem cells, vascular smooth muscle cell differentiation, stem cell heterogeneity, CNN1, SM22α, cell proliferation, collagen matrix contraction, lineage commitment
MeSH terms
Cell Differentiation, Cell Separation, Cells, Cultured, Humans, Molecular Sequence Data, Muscle, Smooth, Vascular, Phenotype, Fibroblast Growth Factor 2, Myocytes, Smooth Muscle, Cell Proliferation, CD146 Antigen, Transforming Growth Factor beta1, Transcriptome, Mesenchymal Stem Cells

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