The Tunica Adventitia of Human Arteries and Veins As a Source of Mesenchymal Stem Cells
Open accessAbstract
We previously demonstrated that human pericytes, which encircle capillaries and microvessels, give rise in culture to genuine mesenchymal stem cells (MSCs). This raised the question as to whether all MSC are derived from pericytes. Pericytes and other cells defined on differential expression of CD34, CD31, and CD146 were sorted from the stromal vascular fraction of human white adipose tissue. Besides pericytes, CD34+ CD31- CD146- CD45- cells, which reside in the outmost layer of blood vessels, the tunica adventitia, natively expressed MSC markers and gave rise in culture to clonogenic multipotent progenitors identical to standard bone marrow-derived MSC. Despite common MSC features and developmental properties, adventitial cells and pericytes retain distinct phenotypes and genotypes through culture. However, in the presence of growth factors involved in vascular remodeling, adventitial cells acquire a pericytes-like phenotype. In conclusion, we demonstrate the co-existence of 2 separate perivascular MSC progenitors: pericytes in capillaries and microvessels and adventitial cells around larger vessels.
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Human perivascular stem cell-derived extracellular vesicles mediate bone repair
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Context-Dependent Development of Lymphoid Stroma from Adult CD34+ Adventitial Progenitors
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- Authors
- Corselli, M., Chen, C.-W., Sun, B., Yap, S., Rubin, J. P., & Péault, B.
- Affiliations
- University of California, Los Angeles (US)
- University of Pittsburgh (US)
- Corresponding author
- Péault, Bruno
- Venue
- Stem Cells and Development
- Publisher
- SAGE Publications
- Published
- 2011-08-23
- Volume
- 21
- Issue
- 8
- Pages
- 1299-1308
- ISSN
- 1525-8165 (ISSN-L)
- 1547-3287
- 1557-8534
- PMC
- PMC3353742
- OpenAlex
- W2147691664
- Cited by
- 393
- References
- 43
- Citations by year
- 2026: 7 · 2025: 10 · 2024: 18 · 2023: 13 · 2022: 16 · 2021: 26 · 2020: 31 · 2019: 41 · 2018: 42 · 2017: 29 · 2016: 38 · 2015: 34 · 2014: 30 · 2013: 28 · 2012: 26
- FWCI
- 14.07
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Stem Cells and Development (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Edinburgh Research Explorer (University of Edinburgh) (submittedVersion, other-oa)
- Funders
- University of Pittsburgh
- National Institutes of Health (TG2-01169)
- Children's Hospital of Pittsburgh
- Topics
- Health Sciences › Medicine › Genetics › Mesenchymal stem cell research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Angiogenesis and VEGF in Cancer
- Health Sciences › Medicine › Hematology › Hematopoietic Stem Cell Transplantation
- Keywords
- mesenchymal stem cells, adventitia, pericytes, adventitial cells, stromal vascular fraction, white adipose tissue, CD34, CD31, CD146, multipotent progenitors, vascular remodeling
- MeSH terms
- Adipose Tissue, Arteries, Cell Separation, Cells, Cultured, Clone Cells, Female, Humans, Immunohistochemistry, Organ Specificity, Veins, Biomarkers, Antigens, CD34, Pericytes, Intercellular Signaling Peptides and Proteins, CD146 Antigen, Mesenchymal Stem Cells
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