CTRP9 knockout exaggerates lipotoxicity in cardiac myocytes and high-fat diet-induced cardiac hypertrophy through inhibiting the LKB1/AMPK pathway
Open accessAbstract
CTRP9 has been reported to regulate lipid metabolism and exert cardioprotective effects, yet its role in high-fat diet (HFD)-induced cardiac lipotoxicity and the underlying mechanisms remain unclear. In the current study, we established HFD-induced obesity model in wild-type (WT) or CTRP9 knockout (CTRP9-KO) mice and palmitate-induced lipotoxicity model in neonatal rat cardiac myocytes (NRCMs) to investigate the effects of CTRP9 on cardiac lipotoxicity. Our results demonstrated that the HFD-fed CTRP9-KO mice accentuated cardiac hypertrophy, fibrosis, endoplasmic reticulum (ER) stress-initiated apoptosis and oxidative stress compared with the HFD-fed WT mice. In vitro, CTRP9 treatment markedly alleviated palmitate-induced oxidative stress and ER stress-induced apoptosis in NRCMs in a dose-dependent manner. Phosphorylated AMPK at Thr172 was reduced, and phosphorylated mammalian target of rapamycin (mTOR) was strengthened in the heart of the HFD-fed CTRP9-KO mice compared with the HFD-fed control mice. In vitro, AMPK inhibitor compound C significantly abolished the effects of CTRP9 on the inhibition of the apoptotic pathway in palmitate-treated NRCMs. In a further mechanistic study, CTRP9 enhanced expression of phosphorylated LKB1 at Ser428 and promoted LKB1 cytoplasmic localization. Besides, silencing of LKB1 gene by lentivirus significantly prohibited activation of AMPK by CTRP9 and partially eliminated the protective effect of CTRP9 on the cardiac lipotoxicity. These results indicate that CTRP9 exerted anti-myocardial lipotoxicity properties and inhibited cardiac hypertrophy probably through the LKB1/AMPK signalling pathway.
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C1q/tumor necrosis factor-related protein 9 exhibited anti-myocardial lipotoxicity effects and attenuated cardiac hypertrophy, likely via activation of the LKB1-AMPK signaling.
“C1q/tumor necrosis factor-related protein 9 exhibited anti-myocardial lipotoxicity effects and attenuated cardiac hypertrophy, likely via activation of the LKB1–AMPK signaling [269].”
From AMPK, Mitochondrial Function, and Cardiovascular Disease (2020) · cites this paper as [269]
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AMPK, Mitochondrial Function, and Cardiovascular Disease
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- Authors
- Zuo, A., Zhao, X., Li, T., Li, J., Lei, S., Chen, J., Xu, D., Song, C., Liu, T., Li, C., & Guo, Y.
- Affiliations
- Qilu Hospital of Shandong University (CN)
- Jinan Central Hospital (CN)
- Corresponding author
- Guo, Yuan
- ORCID
- Venue
- Journal of Cellular and Molecular Medicine
- Publisher
- Wiley
- Published
- 2020-01-13
- Volume
- 24
- Issue
- 4
- Pages
- 2635-2647
- ISSN
- 1582-1838 (ISSN-L)
- 1582-4934
- PMC
- PMC7028852
- OpenAlex
- W3000714448
- Cited by
- 45
- References
- 34
- Citations by year
- 2026: 5 · 2025: 3 · 2024: 4 · 2023: 9 · 2022: 13 · 2021: 8 · 2020: 3
- FWCI
- 5.11
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Journal of Cellular and Molecular Medicine (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Funders
- National Natural Science Foundation of China (8167080802)
- Topics
- Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Cardiac Fibrosis and Remodeling
- Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Cardiovascular Disease and Adiposity
- Health Sciences › Medicine › Epidemiology › Adipokines, Inflammation, and Metabolic Diseases
- Keywords
- CTRP9, cardiac lipotoxicity, cardiac hypertrophy, LKB1/AMPK signaling, high-fat diet, palmitate, cardiomyocyte apoptosis, endoplasmic reticulum stress, oxidative stress, myocardial fibrosis, mTOR, cardioprotection, knockout mice
- MeSH terms
- Animals, Fibrosis, Glycoproteins, Cardiomegaly, Mice, Inbred C57BL, Phosphorylation, Signal Transduction, Apoptosis, Protein Serine-Threonine Kinases, Mice, Knockout, Oxidative Stress, Myocytes, Cardiac, Mice, Adiponectin, AMP-Activated Protein Kinases, Diet, High-Fat, Endoplasmic Reticulum Stress
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