Cited by 27Liver Disease Diagnosis and TreatmentDiet, Metabolism, and DiseaseLiver physiology and pathology

Rodent model of metabolic dysfunction‐associated fatty liver disease: a systematic review

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Cui, Xiao‐Shan, Li, Hong‐Zheng, Li, Liang, Xie, Cheng‐Zhi, Gao, Jia‐Ming, Chen, Yuan‐Yuan, Zhang, Hui‐Yu, Hao, Wei +2 more·2025·Journal of Gastroenterology and Hepatology

Abstract

Although significant progress has been made in developing preclinical models for metabolic dysfunction‐associated steatotic liver disease (MASLD), few have encapsulated the essential biological and clinical outcome elements reflective of the human condition. We conducted a comprehensive literature review of English‐language original research articles published from 1990 to 2023, sourced from PubMed, Embase, and Web of Science, aiming to collate studies that provided a comparative analysis of physiological, metabolic, and hepatic histological characteristics between MASLD models and control groups. The establishment of a robust metabolic dysfunction‐associated steatotic liver rodent model hinges on various factors, including animal species and strains, sex, induction agents and methodologies, and the duration of induction. Through this review, we aim to guide researchers in selecting suitable induction methods and animal species for constructing preclinical models aligned with their specific research objectives and laboratory conditions. Future studies should strive to develop simple, reliable, and reproducible models, considering the model's sensitivity to factors such as light–dark cycles, housing conditions, and environmental temperature. Additionally, the potential of diverse in vitro models, including 3D models and liver organ technology, warrants further exploration as valuable tools for unraveling the cellular mechanisms underlying fatty liver disease.

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  • Induction of MASLD in vitro using FFAs leads to pronounced and quantitatively measurable lipid accumulation in cell lines within 24-72 h, contrasting with animal models where HFD induces steatosis in 8-16 weeks, steatohepatitis in 16-36 weeks, and fibrosis/cirrhosis in 24-52 weeks or more.

    “Induction of MASLD in vitro using free fatty acids leads to a pronounced and quantitatively measurable accumulation of lipids in cell lines within 24–72 h [29].”

    From The HepG2 Cell Line as a Model for Studying Metabolic Dysfunction-Associated Steatotic Liver Disease (2026) · cites this paper as [58]

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  1. [48]Kulkarni KS, Kulkarni YA, Yele VU, Addepalli V. Development of animal model for non alcoholic fatty liver disease based on modified diets. Pharmacology 2014; 3: 126– 135.

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Authors
Cui, X., Li, H., Li, L., Xie, C., Gao, J., Chen, Y., Zhang, H., Hao, W., Fu, J., & Guo, H.
Affiliations
  • Xiyuan Hospital (CN)
  • China Academy of Chinese Medical Sciences (CN)
  • Institute of Basic Medical Sciences, Xiyuan Hospital China Academy of Chinese Medical Sciences Beijing China
Corresponding author
Guo, Hao
Venue
Journal of Gastroenterology and Hepatology
Publisher
Wiley
Published
2024-09-25
Volume
40
Issue
1
Pages
48-66
ISSN
  • 0815-9319 (ISSN-L)
  • 1440-1746
OpenAlex
W4402841284
Cited by
27
References
146
Citations by year
2026: 17 · 2025: 10
FWCI
1.78
Type
journal article
Language
en
Open access
Locations
  • Journal of Gastroenterology and Hepatology (publishedVersion, cc-by-nc)
  • PubMed Central (publishedVersion, cc-by-nc)
  • PubMed (publishedVersion)
Funders
China Academy of Traditional Chinese Medicine
Topics
  • Health Sciences › Medicine › Epidemiology › Liver Disease Diagnosis and Treatment
  • Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Diet, Metabolism, and Disease
  • Health Sciences › Medicine › Hepatology › Liver physiology and pathology
Keywords
non-alcoholic fatty liver disease, rodent models, preclinical animal models, sex differences, liver histopathology, metabolic phenotypes, 3D liver model, liver organoids
MeSH terms
Animals, Disease Models, Animal, Fatty Liver, Female, Humans, Liver, Male, Metabolic Diseases, Mice, Rats, Non-alcoholic Fatty Liver Disease

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