Development of a new eczema-like reconstructed skin equivalent for testing child atopic dermatitis-relieving cosmetics
Abstract
Objective: Atopic dermatitis (AD) is the most common chronic inflammatory skin disease, and it has serious effects on children's and families’ quality of life. We aimed to screen and evaluate the efficacy of different formulas on relieving of atopic dermatitis clinical symptoms by developing an eczema‐like reconstructed human skin equivalent in vitro. Method: Some research has reported that thymic stromal lymphopoietin (TSLP) may be a potential therapeutic target for the treatment of AD. We developed an eczema‐like in vitro skin equivalent by coculturing the cocktails polyinosinic‐polycytidylic acid sodium salt (poly(I:C)) and lipopolysaccharides (LPS). The eczema‐like skin equivalent was characterized by overexpression of TSLP and impaired skin barrier function. Three cosmetic formulas with the potential of anti‐inflammation and skin barrier promotion were topically applied onto the eczema‐like skin equivalent, mimicking in vivo application. The inhibitory effect on TSLP was examined by ELISA. Effects on tissue viability and skin barrier function were determined by 3‐(4,5‐dimethyl‐2‐thiazolyl)‐2,5‐diphenyl‐2‐H‐tetrazolium bromide (MTT) method. Conclusion: The results show that eczema‐like skin equivalent induced by cocktails of poly(I:C) and LPS can mimic the skin characters of the atopic dermatitis. The cocktails can induce high TSLP expression, impaired cell viability, and skin barrier function. The cosmetic formulas with the potential of anti‐inflammation and skin barrier promotion were evaluated to be helpful to decrease and relieve the impact of AD with the decreased TSLP and the higher tissue viability than the eczema‐like skin equivalent without any cosmetic application. The eczema‐like skin equivalent can be used to screen and evaluate formulas on AD relieving.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
HSEs have long served as models of human IFE in place of murine skin.
“HSEs have long served as models of human IFE in place of murine skin.7,18,66,67”
From Single-cell transcriptomics of human-skin-equivalent organoids (2023) · cites this paper as [67]
Cites
Rheinwatd, J. G., & Green, H. (1975) · Cell · doi:10.1016/s0092-8674(75)80001-8 · Cited by 4,528 · see citing works
New insights into atopic dermatitis
Leung, D. Y. M., Boguniewicz, M., Howell, M. D., Nomura, I., & Hamid, Q. A. (2004) · Journal of Clinical Investigation · doi:10.1172/jci21060 · Cited by 1,435 · see citing works
Global variations in prevalence of eczema symptoms in children from ISAAC Phase Three
Odhiambo, J. A., Williams, H. C., Clayton, T. O., Robertson, C. F., & Asher, M. I. (2009) · Journal of Allergy and Clinical Immunology · doi:10.1016/j.jaci.2009.10.009 · Cited by 971 · see citing works
Bieber, T. R. M. (2010) · Annals of Dermatology · doi:10.5021/ad.2010.22.2.125 · Cited by 557 · see citing works
The Burden of Atopic Dermatitis: Impact on the Patient, Family, and Society
Carroll, C. L., Balkrishnan, R., Feldman, S. R., Fleischer, A. B., & Manuel, J. C. (2005) · Pediatric Dermatology · doi:10.1111/j.1525-1470.2005.22303.x · Cited by 489 · see citing works
LEWIS-JONES, S. (2006) · International Journal of Clinical Practice · doi:10.1111/j.1742-1241.2006.01047.x · Cited by 478 · see citing works
Danso, M. O., van Drongelen, V., Mulder, A. A., van Esch, J., Scott, H. C., van Smeden, J., Ghalbzouri, A. E., & Bouwstra, J. A. (2014) · Journal of Investigative Dermatology · doi:10.1038/jid.2014.83 · Cited by 333 · see citing works
Recent insights into atopic dermatitis and implications for management of infectious complications
Boguniewicz, M., & Leung, D. Y. M. (2010) · Journal of Allergy and Clinical Immunology · doi:10.1016/j.jaci.2009.11.027 · Cited by 328 · see citing works
The use of skin models in drug development
Mathes, S. H., Ruffner, H., & Graf‐Hausner, U. (2014) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2013.12.006 · Cited by 321 · see citing works
An update on the genetics of atopic dermatitis: Scratching the surface in 2009
Barnes, K. C. (2010) · Journal of Allergy and Clinical Immunology · doi:10.1016/j.jaci.2009.11.008 · Cited by 310 · see citing works
de Jongh, G. J., Zeeuwen, P. L. J. M., Kucharekova, M., Pfundt, R., van der Valk, P. G., Blokx, W. A. M., Dogan, A., Hiemstra, P. S., Kerkhof, P. C. V. D., & Schalkwijk, J. (2005) · Journal of Investigative Dermatology · doi:10.1111/j.0022-202x.2005.23935.x · Cited by 285 · see citing works
Bogiatzi, S. I., Fernandez, I., Bichet, J.-C., Marloie-Provost, M.-A., Volpe, E., Sastre‐Garau, X., & Soumelis, V. (2007) · The Journal of Immunology · doi:10.4049/jimmunol.178.6.3373 · Cited by 272 · see citing works
The genetics of atopic dermatitis
Morar, N., Willis‐Owen, S. A. G., Moffatt, M. F., & Cookson, W. (2006) · Journal of Allergy and Clinical Immunology · doi:10.1016/j.jaci.2006.03.037 · Cited by 260 · see citing works
Lyons, J. J., Milner, J. D., & Stone, K. D. (2014) · Immunology and Allergy Clinics of North America · doi:10.1016/j.iac.2014.09.008 · Cited by 229 · see citing works
Skin-Derived TSLP Triggers Progression from Epidermal-Barrier Defects to Asthma
Demehri, S., Morimoto, M., Holtzman, M. J., & Kopan, R. (2009) · PLoS Biology · doi:10.1371/journal.pbio.1000067 · Cited by 227 · see citing works
Augustine, R. (2018) · Progress in Biomaterials · doi:10.1007/s40204-018-0087-0 · Cited by 184 · see citing works
The Genetics and Epigenetics of Atopic Dermatitis—Filaggrin and Other Polymorphisms
Liang, Y., Chang, C., & Lu, Q. (2015) · Clinical Reviews in Allergy & Immunology · doi:10.1007/s12016-015-8508-5 · Cited by 166 · see citing works
Pendaries, V., Malaisse, J., Pellerin, L., Lamer, M. L., Nachat, R., Kežić, S., Schmitt, A.-M., Paul, C. F., Poumay, Y., Serre, G., et al. (2014) · Journal of Investigative Dermatology · doi:10.1038/jid.2014.259 · Cited by 143 · see citing works
Barrier Dysfunction Caused by Environmental Proteases in the Pathogenesis of Allergic Diseases
Takai, T., & Ikeda, S. (2011) · Allergology International · doi:10.2332/allergolint.10-rai-0273 · Cited by 142 · see citing works
Kinoshita, H., Takai, T., Lê, T. A., Kamijo, S., Wang, X. L., Ushio, H., Hara, M., Kawasaki, J., Vu, A. T., Ogawa, T., et al. (2008) · Journal of Allergy and Clinical Immunology · doi:10.1016/j.jaci.2008.10.008 · Cited by 131 · see citing works
Advancement of wound care from grafts to bioengineered smart skin substitutes
Augustine, R., Kalarikkal, N., & Thomas, S. (2014) · Progress in Biomaterials · doi:10.1007/s40204-014-0030-y · Cited by 130 · see citing works
McAleer, M. A., Jakaša, I., Hurault, G., Sarvari, P., McLean, W. H. I., Tanaka, R. J., Kežić, S., & Irvine, A. D. (2018) · British Journal of Dermatology · doi:10.1111/bjd.17088 · Cited by 106 · see citing works
α-(-)-bisabolol Reduces Pro-inflammatory Cytokine Production and Ameliorates Skin Inflammation
Maurya, A. K., Singh, M., Dubey, V., Srivastava, S., Luqman, S., & Bawankule, D. U. (2014) · Current Pharmaceutical Biotechnology · doi:10.2174/1389201015666140528152946 · Cited by 99 · see citing works
Margolis, D. J., Kim, B., Apter, A. J., Gupta, J., Hoffstad, O., Papadopoulos, M., & Mitra, N. (2014) · JAMA Dermatology · doi:10.1001/jamadermatol.2013.7954 · Cited by 96 · see citing works
Insights into atopic dermatitis gained from genetically defined mouse models
Nakajima, S., Nomura, T., Common, J., & Kabashima, K. (2019) · Journal of Allergy and Clinical Immunology · doi:10.1016/j.jaci.2018.11.014 · Cited by 80 · see citing works
In vitro reproduction of clinical hallmarks of eczematous dermatitis in organotypic skin models
Engelhart, K., Hindi, T. E., Biesalski, H. K., & Pfitzner, I. (2005) · Archives of Dermatological Research · doi:10.1007/s00403-005-0575-7 · Cited by 53 · see citing works
Bioengineered Skin Intended for Skin Disease Modeling
Sarkiri, M., Fox, S., Fratila-Apachitei, L. E., & ZADPOOR, A. A. (2019) · International Journal of Molecular Sciences · doi:10.3390/ijms20061407 · Cited by 45 · see citing works
Reconstructed human epidermis for in vitro studies on atopic dermatitis: A review
Huet, F., Severino‐Freire, M., Chéret, J., Gouin, O., Praneuf, J., Pierre, O., Misery, L., & Gall‐Ianotto, C. L. (2017) · Journal of Dermatological Science · doi:10.1016/j.jdermsci.2017.11.015 · Cited by 39 · see citing works
Advances in atopic dermatitis in 2017
Kennedy, K. L., Heimall, J. R., & Spergel, J. M. (2018) · Journal of Allergy and Clinical Immunology · doi:10.1016/j.jaci.2018.10.012 · Cited by 25 · see citing works
Genetic Risk Factors for Development of Atopic Dermatitis: a Systematic Review
Yazd, N. K. K., Patel, R. R., Dellavalle, R. P., & Dunnick, C. A. (2017) · Current Dermatology Reports · doi:10.1007/s13671-017-0199-0 · Cited by 7 · see citing works
Overall perspective on the clinical importance of skin models
Yousuf, Y., Amini‐Nik, S., & Jeschke, M. G. (2017) · Elsevier eBooks · doi:10.1016/b978-0-12-810545-0.00002-4 · Cited by 7 · see citing works
Flagged first, then by citations
1 of the 32 references couldn’t be matched to a known paper.
Cited by
Sorted by citations
Single-cell transcriptomics of human-skin-equivalent organoids
Stabell, A. R., Lee, G. E., Jia, Y., Wong, K. N., Wang, S., Ling, J., Nguyen, S. D., Sen, G. L., Nie, Q., & Atwood, S. X. (2023) · Cell Reports · doi:10.1016/j.celrep.2023.112511 · Cited by 30 · see citing works
Borgia, F., Custurone, P., Peterle, L., Pioggia, G., & Gangemi, S. (2021) · Biomolecules · doi:10.3390/biom11121843 · Cited by 22 · see citing works
Keren, A., Reich, K., Bertolini, M., Moga, A., Riethmüller, C., Ullmann, Y., Paus, R., & Gilhar, A. (2023) · Allergy · doi:10.1111/all.15635 · Cited by 12 · see citing works
Zhang, X., Xu, H., Hua, J., Zhu, Z., & Wang, M. (2022) · Chemistry & Biodiversity · doi:10.1002/cbdv.202200205 · Cited by 12 · see citing works
Nanocosmeceuticals: Concept, opportunities, and challenges
Das, S., Das, S., & Das, M. K. (2022) · Elsevier eBooks · doi:10.1016/b978-0-323-91077-4.00008-9 · Cited by 7 · see citing works
Wu, R., Wu, H., Wang, Y., & Li, L. (2023) · Desalination and Water Treatment · doi:10.5004/dwt.2023.29130 · Cited by 4 · see citing works
Liu, X., Hage, T., Chen, L.-C., Wang, E. H. C., Liao, I., Goldberg, J., Gosto, S., Cziryak, P., Senna, M. M., Chen, Y., et al. (2024) · Journal of Cosmetic Dermatology · doi:10.1111/jocd.16662 · Cited by 4 · see citing works
Răileanu, M., Tihăuan, B.-M., Lixandru-Petre, I.-O., Gheorghiu, G.-L., Pîrcălăbioru, G. G., Cioca, G., Iliescu, F. S., & Iliescu, C. (2025) · APL Bioengineering · doi:10.1063/5.0268706 · Cited by 1 · see citing works
- Authors
- Gu, H., Zhu, Y., Jia, T., Li, X., Lu, Y., & Kaku, K.
- Affiliations
- Pigeon Manufacturing (Shanghai) Co., Ltd. Shanghai China
- Guangdong Biocell Biotechnology, Co.,Ltd. Dongguan China
- Corresponding author
- Kaku, Ken
- Venue
- Journal of Cosmetic Dermatology
- Publisher
- Wiley
- Published
- 2019-07-17
- Volume
- 19
- Issue
- 3
- Pages
- 752-757
- ISSN
- 1473-2130 (ISSN-L)
- 1473-2165
- OpenAlex
- W2957656037
- Cited by
- 8
- References
- 32
- Citations by year
- 2025: 1 · 2024: 1 · 2023: 3 · 2022: 2 · 2021: 1
- FWCI
- 0.76
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Cosmetic Dermatology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Dermatology › Dermatology and Skin Diseases
- Health Sciences › Medicine › Dermatology › Contact Dermatitis and Allergies
- Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Pharmaceutical Science › Advancements in Transdermal Drug Delivery
- Keywords
- atopic dermatitis, in vitro skin model, thymic stromal lymphopoietin, poly(I:C), lipopolysaccharide, skin barrier function, pediatric dermatitis
- MeSH terms
- Cosmeceuticals, Thymic Stromal Lymphopoietin, Anti-Inflammatory Agents, Child, Culture Media, Dermatitis, Atopic, Drug Evaluation, Preclinical, Epidermis, Humans, Lipopolysaccharides, Permeability, Poly I-C, Keratinocytes, Cytokines, Coculture Techniques, Cell Culture Techniques
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
