Consensus report on the use of PN-HPT™ (polynucleotides highly purified technology) in aesthetic medicine
Open accessAbstract
Background: Injective procedures using polynucleotides‐based products to promote dermal rejuvenation and revitalization are steadily evolving, yet no structured protocols are available that discuss and provide guidance in aesthetic treatments with highly purified polynucleotides. The goal of this document was to provide consensus‐based recommendations for the safe and effective use of Polynucleotides Highly Purified Technology™ (PN‐HPT™) devices for skin rejuvenation. Patients/Methods: A team of eight experts with extensive experience in treatments for skin rejuvenation and revitalization integrated the best available evidence and clinical judgment and devised a series of practical guidance to support dermatologists, plastic surgeons, and aesthetic physician in the use of PN‐HPT™ products, alone and in combination, in aesthetic medicine. Results: For most items, the expert group achieved a majority consensus. “Recommendations” (consensus >80%) were reached for the face, periocular area, décolleté and neck, hands, scalp, and stretch marks. Recommendations include details of techniques, information on dosage, volumes to be injected, and the ideal number of required treatment sessions, as well as time intervals between them for different areas of face and body. A lower agreement level of 60% was reached on but one item related to the initial treatment cycle for the face, leading to a “Consensus statement” for that area instead of a full “Recommendation.” Conclusion: The expert consensus illustrates the value of natural‐origin, highly purified polynucleotides (PN‐HPT™) as biostimulatory booster strategy for skin priming and revitalization of face and body and provides a detailed guide for the use.
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Products from alternative biological source approaches for PDRN production are often safeguarded by patents.
“and the products are often safeguarded by patents [134,163,164]”
From Comparison of Polynucleotide and Polydeoxyribonucleotide in Dermatology: Molecular Mechanisms and Clinical Perspectives (2025) · cites this paper as [164]
The Conflict of Interest section says Mastelli holds patents for PN-HPT™ and produces PN-HPT™-based medical devices. The Background identifies polynucleotides as extracted from trout gonads, but the paper does not establish that products from alternative biological sources are often patented. (LLM, conf=0.86)
Experimental — accuracy not yet measuredAlternative biological sources such as plants, algae, and sea cucumbers have been explored for PDRN production.
“Alternative approaches using biological sources such as plants, algae, and sea cucumbers have also been explored for PDRN production”
From Comparison of Polynucleotide and Polydeoxyribonucleotide in Dermatology: Molecular Mechanisms and Clinical Perspectives (2025) · cites this paper as [164]
The Background (page unavailable) describes the polynucleotides in this report as DNA extracted from trout gonads. Neither that passage nor the additional passages found by searching for plants, algae, sea cucumbers, and alternative biological sources addresses whether those sources have been explored for PDRN production. (LLM, conf=0.75)
Experimental — accuracy not yet measuredAlternative biological source approaches for PDRN production tend to be more complex.
“although these approaches tend to be more complex”
From Comparison of Polynucleotide and Polydeoxyribonucleotide in Dermatology: Molecular Mechanisms and Clinical Perspectives (2025) · cites this paper as [164]
The Background section identifies trout gonads as a source of purified polynucleotides, but does not compare biological sources or discuss the complexity of PDRN production. Searches for alternative sources and production complexity found no passage addressing that comparison; page numbers were unavailable in our copy. (LLM, conf=0.74)
Experimental — accuracy not yet measured
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Research works printed but not linked: 15
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Cited by
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The effectiveness of polynucleotides in esthetic medicine: a systematic review
Lampridou, S., Bassett, S., Cavallini, M., & Christopoulos, G. (2025) · Journal of Cosmetic Dermatology · doi:10.1111/jocd.16721 · Cited by 41 · see citing works
Self-citationAbuyousif, H. S., Porcello, A., Cerrano, M., Marques, C., Scaletta, C., Lourenço, K., Abdel-Sayed, P., Chemali, M., Raffoul, W., Hirt-Burri, N., et al. (2025) · Polymers · doi:10.3390/polym17050638 · Cited by 19 · see citing works
Kim, S. T. (2025) · Pharmaceutics · doi:10.3390/pharmaceutics17081024 · Cited by 18 · see citing works
- Authors
- Cavallini, M., Bartoletti, E., Maioli, L., Massirone, A., Pia Palmieri, I., Papagni, M., Priori, M., Trocchi, G., & As Members of The Polynucleotides HPT™ Priming Board, C. I. D. S. S. D. M. E. (. C. O. T. A. M. S. S. —. S.
- Affiliations
- Centro Diagnostico Italiano (IT)
- Fatebenefratelli Hospital (IT)
- Veterinary Oncological Center (IT)
- Agorà President, Italian Scientific Society of Aesthetic Medicine Milan Italy
- Contract Professor, Esthetic Medicine Master, “Federico II” University Medical School Naples Italy
- Member of the Board Agorà Italian Scientific Society of Aesthetic Medicine Milan Italy
- President, SIES, Esthetic Medicine and Surgery Society Florence Italy
- Corresponding author
- Cavallini, Maurizio
- Venue
- Journal of Cosmetic Dermatology
- Publisher
- Wiley
- Published
- 2020-09-21
- Volume
- 20
- Issue
- 3
- Pages
- 922-928
- ISSN
- 1473-2130 (ISSN-L)
- 1473-2165
- PMC
- PMC7984045
- OpenAlex
- W3049572794
- Cited by
- 70
- References
- 0
- Citations by year
- 2026: 24 · 2025: 21 · 2024: 10 · 2023: 8 · 2022: 5 · 2021: 2
- FWCI
- 2.74
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Cosmetic Dermatology (publishedVersion, cc-by-nc-nd)
- PubMed Central (publishedVersion, cc-by-nc-nd)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Dermatology › Facial Rejuvenation and Surgery Techniques
- Health Sciences › Medicine › Dermatology › Dermatologic Treatments and Research
- Health Sciences › Medicine › Urology › Periodontal Regeneration and Treatments
- Keywords
- skin rejuvenation, aesthetic medicine, intradermal injection, treatment protocols, treatment interval, hand, scalp, striae, face
- MeSH terms
- Cosmetic Techniques, Esthetics, Humans, Hyaluronic Acid, Polynucleotides, Rejuvenation, Technology, Skin Aging, Consensus
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