Fatty Acid Content and Profile of the Novel Strain of Coccomyxa elongata (Trebouxiophyceae, Chlorophyta) Cultivated at Reduced Nitrogen and Phosphorus Concentrations
Abstract
A novel freshwater strain of Coccomyxa elongata (MZ–Ch64) was isolated from the Zaporizhia region, Ukraine. The identification was based on the phylogenetic analysis of SSU rDNA gene and ITS 1–5.8S rDNA–ITS 2 region and predicted secondary structure of the ITS 2. Phylogenetic analysis placed this strain in the Coccomyxa group, within the class Trebouxiophyceae. The novel strain MZ–Ch64 formed a strongly supported lineage closest with C. elongata. The MZ–Ch64 strain differed from the morphological description of the species by the size of vegetative cells and absence of small mucilaginous caps at one end of the cell. A number of experiments with different concentrations of phosphate and nitrate were conducted to evaluate changes in the resulting fatty acid profiles and biomass productivity. The fatty acid profile and total fatty acids varied significantly under different nutrient deficiencies. The dominant fatty acid during cultivation on standard BBM medium, as well as in phosphorus‐depleted conditions, was oleic acid (to 48.0%–54.6% of total fatty acids). Absence of nitrogen alone, and absence of both nitrogen and phosphorus, led to an increase of palmitic acid (to 24.7%–25.6%), cis‐7‐hexadecenoic acid (to 14.8%) and α‐linolenic acid (to 9.1%–10.1%) in comparison with the control sample. The greatest variation was found for oleic acid (31.9%–54.6%). Thus, this strain can be considered as a potential producer of oleic acid or cis‐7‐hexadecenoic and α‐linolenic acids for biotechnological applications.
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Changes in fatty acid composition under nitrogen deficiency involved notable increases in SFA and MUFA, accompanied by a decrease in PUFA.
“In general, these changes involved notable increases in SFA and MUFA, accompanied by a decrease in PUFA.”
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- Authors
- Maltsev, Y., Maltseva, I., Maltseva, S., Kociolek, J. P., & Kulikovskiy, M.
- Affiliations
- Timiryazev Institute of Plant Physiology (RU)
- Bogdan Khmelnitsky Melitopol State Pedagogical University (UA)
- University of Colorado Boulder (US)
- Corresponding author
- Maltsev, Yevhen
- Kulikovskiy, Maxim
- Venue
- Journal of Phycology
- Publisher
- Wiley
- Published
- 2019-08-28
- Volume
- 55
- Issue
- 5
- Pages
- 1154-1165
- ISSN
- 0022-3646 (ISSN-L)
- 1529-8817
- OpenAlex
- W2964332420
- Cited by
- 28
- References
- 47
- Citations by year
- 2026: 4 · 2025: 3 · 2024: 2 · 2023: 6 · 2022: 3 · 2021: 7 · 2020: 2
- FWCI
- 0.64
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Phycology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Russian Science Foundation (18‐74‐00095)
- Topics
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
- Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Aquaculture Nutrition and Growth
- Keywords
- Trebouxiophyceae, Chlorophyta, fatty acid composition, oleic acid, phosphorus deficiency, nitrogen deficiency, nutrient limitation, phylogenetic identification, ITS2 secondary structure, biomass productivity, alpha-linolenic acid
- MeSH terms
- Chlorophyta, Fatty Acids, Nitrogen, Phosphorus, Phylogeny, Biomass, Microalgae
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