Gut microbiota dysbiosis in patients with hepatitis B virus–induced chronic liver disease covering chronic hepatitis, liver cirrhosis and hepatocellular carcinoma
Abstract
The information regarding the effect of hepatitis B virus (HBV) infection on gut microbiota and the relationship between gut microbiota dysbiosis and hepatitis B virus-induced chronic liver disease (HBVCLD) is limited. In this study, we aimed at characterizing the gut microbiota composition in the three different stages of hepatitis B virus-induced chronic liver disease patients and healthy individuals. Faecal samples and clinical data were collected from HBVCLD patients and healthy individuals. The 16S rDNA gene amplification products were sequenced. Bioinformatic analysis including alpha diversity and PICRUSt was performed. A total of 19 phyla, 43 classes, 72 orders, 126 families and 225 genera were detected. The beta-diversity showed a separate clustering of healthy controls and HBVCLD patients covering chronic hepatitis (CHB), liver cirrhosis (LC) and hepatocellular carcinoma (HCC); and gut microbiota of healthy controls was more consistent, whereas those of CHB, LC and HCC varied substantially. The abundance of Firmicutes was lower, and Bacteroidetes was higher in patients with CHB, LC and HCC than in healthy controls. Predicted metagenomics of microbial communities showed an increase in glycan biosynthesis and metabolism-related genes and lipid metabolism-related genes in HBVCLD than in healthy individuals. Our study suggested that HBVCLD is associated with gut dysbiosis, with characteristics including, a gain in potential bacteria and a loss in potential beneficial bacteria or genes. Further study of CHB, LC and HCC based on microbiota may provide a novel insight into the pathogenesis of HBVCLD as well as a novel treatment strategy.
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Dysbiosis associated with HepB infection involves a gain in potentially pathogenic bacteria (e.g., some Enterobacteriaceae) and a loss in potentially beneficial bacteria, namely Bifidobacterium species.
“The dysbiosis associated with HepB infection appears to involve a gain in potentially pathogenic bacteria (e.g., some Enterobacteriaceae) and a loss in potentially beneficial bacteria; namely Bifidobacterium species (Zeng et al., 2020).”
From Viral Infections, the Microbiome, and Probiotics (2021) · cites this paper as Zeng et al., 2020
Protective mechanisms offered by bifidobacteria include reduction in endotoxin levels, compositional modulation of gut microbiota, antimicrobial production, enhanced GI barrier function and modulation of local and systemic immunity.
“The protective mechanisms offered by bifidobacteria include reduction in endotoxin levels (GI and plasma), compositional modulation of gut microbiota, antimicrobial production, enhanced GI barrier function and modulation of local and systemic immunity (Zeng et al., 2020).”
From Viral Infections, the Microbiome, and Probiotics (2021) · cites this paper as Zeng et al., 2020
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Viral Infections, the Microbiome, and Probiotics
Harper, A., Vijayakumar, V., Ouwehand, A. C., ter Haar, J., Obis, D., Espadaler, J., Binda, S., Desiraju, S., & Day, R. (2021) · Frontiers in Cellular and Infection Microbiology · doi:10.3389/fcimb.2020.596166 · Cited by 177 · see citing works
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- Authors
- Zeng, Y., Chen, S., Fu, Y., Wu, W., Chen, T., Chen, J., Yang, B., & Ou, Q.
- Affiliations
- Fujian Medical University (CN)
- First Affiliated Hospital of Fujian Medical University (CN)
- Corresponding author
- Yang, Bin
- Ou, Qishui
- Venue
- Journal of Viral Hepatitis
- Publisher
- Wiley
- Published
- 2019-10-29
- Volume
- 27
- Issue
- 2
- Pages
- 143-155
- ISSN
- 1352-0504 (ISSN-L)
- 1365-2893
- OpenAlex
- W2979620955
- Cited by
- 148
- References
- 26
- Citations by year
- 2026: 8 · 2025: 16 · 2024: 21 · 2023: 35 · 2022: 23 · 2021: 27 · 2020: 18
- FWCI
- 6.37
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Viral Hepatitis (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Natural Science Foundation of China (81601834, 81702073, 81772251)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Health Sciences › Medicine › Epidemiology › Hepatitis B Virus Studies
- Health Sciences › Dentistry › Periodontics › Oral microbiology and periodontitis research
- Keywords
- gut microbiota, dysbiosis, hepatitis B virus, chronic liver disease, chronic hepatitis B, liver cirrhosis, hepatocellular carcinoma, Firmicutes, Bacteroidetes, glycan metabolism, lipid metabolism, microbiome diversity
- MeSH terms
- Gastrointestinal Microbiome, Adult, Bacteria, Feces, Female, Hepatitis B virus, Carcinoma, Hepatocellular, Humans, Liver Cirrhosis, Liver Neoplasms, Male, Middle Aged, RNA, Ribosomal, 16S, Case-Control Studies, Hepatitis B, Chronic, Metagenomics, Dysbiosis
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