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Cited by 39Fungal and yeast genetics researchAntifungal resistance and susceptibilityFungal Infections and Studies

Bcr 1 plays a central role in the regulation of opaque cell filamentation in C andida albicans

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Guan, Guobo, Xie, Jing, Tao, Li, Nobile, Clarissa J., Sun, Yuan, Cao, Chengjun, Tong, Yaojun, Huang, Guanghua·2013·Molecular Microbiology
DOI 10.1111/mmi.12310PMC PMC3758918
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Abstract

Summary: The human fungal pathogen C andida albicans has at least two types of morphological transitions: white to opaque cell transitions and yeast to hyphal transitions. Opaque cells have historically not been known to undergo filamentation under standard filament‐inducing conditions. Here we find that Bcr 1 and its downstream regulators Cup 9, Nrg 1 and Czf 1 and the cAMP‐signalling pathway control opaque cell filamentation in C. albicans. We have shown that deletion of BCR1, CUP9, NRG1 and CZF1 results in opaque cell filamentation under standard culture conditions. Disruption of BCR1 in white cells has no obvious effect on hyphal growth, suggesting that Bcr 1 is an opaque‐specific regulator of filamentation under the conditions tested. Moreover, inactivation of the cAMP‐signalling pathway or disruption of its downstream transcriptional regulators, FLO8 and EFG1, strikingly attenuates filamentation in opaque cells of the bcr1/bcr1 mutant. Deletion of HGC1, a downstream gene of the cAMP‐signalling pathway encoding G 1 cyclin‐related protein, completely blocks opaque cell filamentation induced by inactivation of BCR1. These results demonstrate that Bcr 1 regulated opaque cell filamentation is dependent on the cAMP‐signalling pathway. This study establishes a link between the white‐opaque switch and the yeast‐filamentous growth transition in C. albicans.

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  • Gfc1, the homolog of Candida albicans Bcr1, plays a critical regulatory role in hyphal formation and biofilm development in pathogenic Candida species.

    “It has been known that the Gfc1 (the homolog of C. albicans Bcr1) transcription factor plays a critical regulatory role in hyphal formation and biofilm development in pathogenic Candida species29,47,48.”

    From The Wor2 phenotypic switching regulator controls biofilm formation in Candida auris (2026) · cites this paper as [47]

    The Discussion (page not available) describes Bcr1 as a regulator of biofilm development and reports this study’s finding that it regulates opaque-cell filamentation in C. albicans. The copy does not identify Gfc1 as a Bcr1 homolog or establish that Gfc1 regulates hyphal formation and biofilm development across pathogenic Candida species; a search for Gfc1 found no matching passage. (LLM, conf=0.87)

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Cited by

Sorted by citations

Authors
Guan, G., Xie, J., Tao, L., Nobile, C. J., Sun, Y., Cao, C., Tong, Y., & Huang, G.
Affiliations
  • Chinese Academy of Sciences (CN)
  • State Key Laboratory of Mycology Institute of Microbiology Chinese Academy of Sciences Beijing 100101 China
  • University of California, San Francisco (US)
Corresponding author
Huang, Guanghua
ORCID
Venue
Molecular Microbiology
Publisher
Wiley
Published
2013-07-12
Volume
89
Issue
4
Pages
732-750
ISSN
  • 0950-382X (ISSN-L)
  • 1365-2958
DOI
10.1111/mmi.12310
PMC
PMC3758918
OpenAlex
W1839352522
Cited by
39
References
3
Citations by year
2026: 1 · 2025: 2 · 2024: 4 · 2023: 1 · 2022: 2 · 2021: 3 · 2020: 5 · 2019: 2 · 2017: 3 · 2016: 4 · 2015: 7 · 2014: 3 · 2013: 2
FWCI
2.39
Type
journal article
Language
en
Open access
Licence
onlinelibrary.wiley.com/termsAndConditions#vor (vor)
Locations
  • Molecular Microbiology (publishedVersion)
  • PubMed (publishedVersion)
  • PubMed Central (acceptedVersion)
Funders
  • National Natural Science Foundation of China
  • Chinese Academy of Sciences
  • National Institutes of Health
  • National Institute of Allergy and Infectious Diseases (K99AI00896, R01AI049187, K99AI100896)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
  • Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
  • Health Sciences › Medicine › Epidemiology › Fungal Infections and Studies
Keywords
BCR1, Candida albicans, cell filamentation, neuregulin-1, FLO8, EFG1, yeast-to-hypha transition, fungal morphogenesis
MeSH terms
Cyclic AMP, Candida albicans, Fungal Proteins, Signal Transduction, Gene Expression Regulation, Fungal, Gene Deletion, Hyphae

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