A mechanical signal transmitted by the flagellum controls signalling in B acillus subtilis

Cairns, Lynne S.; Marlow, Victoria L.; Bissett, Emma; Ostrowski, Adam; Stanley‐Wall, Nicola R.

doi:10.1111/mmi.12342

Cited by 143Bacterial Genetics and BiotechnologyBacterial biofilms and quorum sensingBiochemical and Structural Characterization

A mechanical signal transmitted by the flagellum controls signalling in B acillus subtilis

Open access

Cairns, Lynne S., Marlow, Victoria L., Bissett, Emma, Ostrowski, Adam, Stanley‐Wall, Nicola R.·2013·Molecular Microbiology

DOI 10.1111/mmi.12342PMC PMC3963450

Open this paper in CoChat — free account Open PDF

Abstract

Summary: In the natural environment bacteria predominantly live adhered to a surface as part of a biofilm. While many of the components needed for biofilm assembly are known, the mechanism by which microbes sense and respond to contact with a surface is poorly understood. B acillus subtilis is a G ram‐positive model for biofilm formation. The DegS – DegU two‐component system controls several multicellular behaviours in B . subtilis , including biofilm formation. Here we identify the B . subtilis flagellum as a mechanosensor that activates the DegS – DegU regulatory pathway. Inhibition of flagellar rotation by deletion or mutation of the flagellar stator gene, motB , results in an increase in both degU transcription and DegU ∼ P driven processes, namely exoprotease production and poly‐γ‐ dl ‐glutamic acid biosynthesis. Similarly, inhibition of flagellar rotation by engaging the flagellar clutch or by tethering the flagella with antibodies also promotes an increase in degU transcription that is reflective of increased DegU ∼ P levels in the cell. Collectively, these findings strongly indicate that inhibition of flagellar rotation acts as a mechanical trigger to activate the DegS – DegU two‐component signal transduction system. We postulate that inhibition of flagellar rotation could function as a mechanical trigger to activate bacterial signal transduction cascades in many motile bacteria upon contact with a surface.

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

Integrity signals

Registry

Crossref · 10.1111/mmi.12342

Cites

98 of 98 references verified0 citation errors · 0 not checkable

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

The DegS-DegU two component system can trigger biofilm formation in response to impeded flagellar rotation.
“The top-ranked module for the CMDs and liver cirrhosis was the ACVD-microbiome depleted DegS-DegU two component system, which can trigger biofilm formation in response to impeded flagellar rotation49.”
From The gut microbiome in atherosclerotic cardiovascular disease (2017) · cites this paper as [49]

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Experiments in molecular genetics
Miller, J. H. (1972) · Medical Entomology and Zoology · Cited by 27,157 · see citing works
The biofilm matrix
Flemming, H.-C., & Wingender, J. (2010) · Nature Reviews Microbiology · doi:10.1038/nrmicro2415 · Cited by 10,137 · see citing works
The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers
Vieira, J., & Messing, J. W. (1982) · Gene · doi:10.1016/0378-1119(82)90015-4 · Cited by 6,127 · see citing works
MICROBIAL BIOFILMS
Costerton, J. W., Lewandowski, Z., Caldwell, D. E., Korber, D. R., & Lappin-Scott, H. M. (1995) · Annual Review of Microbiology · doi:10.1146/annurev.mi.49.100195.003431 · Cited by 4,718 · see citing works
Biofilm formation as microbial development
O'Toole, G., Kaplan, H. B., & Kolter, R. (2000) · Annual Review of Microbiology · doi:10.1146/annurev.micro.54.1.49 · Cited by 3,571 · see citing works
Flagellar and twitching motility are necessary for Pseudomonas aeruginosa biofilm development
O'Toole, G. A., & Kolter, R. (1998) · Molecular Microbiology · doi:10.1046/j.1365-2958.1998.01062.x · Cited by 2,697 · see citing works
Genetic analysis of Escherichia coli biofilm formation: roles of flagella, motility, chemotaxis and type I pili
Pratt, L. A., & Kolter, R. (1998) · Molecular Microbiology · doi:10.1046/j.1365-2958.1998.01061.x · Cited by 1,641 · see citing works
Biofilm, City of Microbes
Watnick, P. I., & Kolter, R. (2000) · Journal of Bacteriology · doi:10.1128/jb.182.10.2675-2679.2000 · Cited by 1,434 · see citing works
Fruiting body formation by Bacillus subtilis
Branda, S. S., González-Pastor, J. E., Ben-Yehuda, S., Losick, R., & Kolter, R. (2001) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.191384198 · Cited by 1,129 · see citing works
Sticking together: building a biofilm the Bacillus subtilis way
Vlamakis, H. C., Chai, Y., Beauregard, P. B., Losick, R. M., & Kolter, R. (2013) · Nature Reviews Microbiology · doi:10.1038/nrmicro2960 · Cited by 1,022 · see citing works
New Vector for Efficient Allelic Replacement in Naturally Nontransformable, Low-GC-Content, Gram-Positive Bacteria
Arnaud, M., Chastanet, A., & Débarbouillé, M. (2004) · Applied and Environmental Microbiology · doi:10.1128/aem.70.11.6887-6891.2004 · Cited by 953 · see citing works
Initiation of sporulation in B. subtilis is controlled by a multicomponent phosphorelay
Burbulys, D., Trach, K. A., & Hoch, J. A. (1991) · Cell · doi:10.1016/0092-8674(91)90238-t · Cited by 873 · see citing works
BiofilmsPreprint
López, D., Vlamakis, H. C., & Kolter, R. (2010) · Cold Spring Harbor Perspectives in Biology · doi:10.1101/cshperspect.a000398 · Cited by 796 · see citing works
Coordinating assembly of a bacterial macromolecular machine
Chevance, F. F. V., & Hughes, K. T. (2008) · Nature Reviews Microbiology · doi:10.1038/nrmicro1887 · Cited by 734 · see citing works
A major protein component of the Bacillus subtilis biofilm matrix
Branda, S. S., Chu, F., Kearns, D. B., Losick, R. M., & Kolter, R. (2005) · Molecular Microbiology · doi:10.1111/j.1365-2958.2005.05020.x · Cited by 707 · see citing works
Steps in the development of a Vibrio cholerae El Tor biofilm
Watnick, P. I., & Kolter, R. (1999) · Molecular Microbiology · doi:10.1046/j.1365-2958.1999.01624.x · Cited by 623 · see citing works
Regulation of flagellar motility during biofilm formation
Guttenplan, S. B., & Kearns, D. B. (2013) · FEMS Microbiology Reviews · doi:10.1111/1574-6976.12018 · Cited by 619 · see citing works
Flagellar Motility Is Critical for Listeria monocytogenes Biofilm Formation
Lemon, K. P., Higgins, D. E., & Kolter, R. (2007) · Journal of Bacteriology · doi:10.1128/jb.01967-06 · Cited by 464 · see citing works
The sporulation transcription factor Spo0A is required for biofilm development in Bacillus subtilis
Hamon, M. A., & Lazazzera, B. A. (2001) · Molecular Microbiology · doi:10.1046/j.1365-2958.2001.02709.x · Cited by 448 · see citing works
Second Messenger-Mediated Adjustment of Bacterial Swimming Velocity
Boehm, A., Kaiser, M., Li, H., Spangler, C., Kasper, C. A., Ackermann, M., Kaever, V., Sourjik, V., Röth, V., & Jenal, U. (2010) · Cell · doi:10.1016/j.cell.2010.01.018 · Cited by 441 · see citing works
High- and Low-Threshold Genes in the Spo0A Regulon of Bacillus subtilis
Fujita, M., González‐Pastor, J. E., & Losick, R. M. (2005) · Journal of Bacteriology · doi:10.1128/jb.187.4.1357-1368.2005 · Cited by 428 · see citing works
A protonmotive force drives bacterial flagella.
Manson, M. D., Tedesco, P., Berg, H. C., Harold, F. M., & van der DRIFT, C. (1977) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.74.7.3060 · Cited by 423 · see citing works
The c-di-GMP Binding Protein YcgR Controls Flagellar Motor Direction and Speed to Affect Chemotaxis by a “Backstop Brake” Mechanism
Paul, K., Nieto, V., Carlquist, W. C., Blair, D. F., & Harshey, R. M. (2010) · Molecular Cell · doi:10.1016/j.molcel.2010.03.001 · Cited by 403 · see citing works
Structurally diverse natural products that cause potassium leakage trigger multicellularity in Bacillus subtilis
López, D., Fischbach, M. A., Chu, F., Losick, R. M., & Kolter, R. (2008) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0810940106 · Cited by 392 · see citing works
Generation of multiple cell types in Bacillus subtilis
López, D., Vlamakis, H. C., & Kolter, R. (2008) · FEMS Microbiology Reviews · doi:10.1111/j.1574-6976.2008.00148.x · Cited by 384 · see citing works
Cell population heterogeneity during growth of Bacillus subtilisPreprint
Kearns, D. B., & Losick, R. M. (2005) · Genes & Development · doi:10.1101/gad.1373905 · Cited by 359 · see citing works
Microanatomy at Cellular Resolution and Spatial Order of Physiological Differentiation in a Bacterial Biofilm
Serra, D. O., Richter, A. M., Klauck, G., Mika, F., & Hengge, R. (2013) · mBio · doi:10.1128/mbio.00103-13 · Cited by 348 · see citing works
Poly‐gamma‐glutamate in bacteria
Candela, T., & Fouet, A. (2006) · Molecular Microbiology · doi:10.1111/j.1365-2958.2006.05179.x · Cited by 341 · see citing works
Electrostatic interactions between rotor and stator in the bacterial flagellar motor
Zhou, J., Lloyd, S. A., & Blair, D. F. (1998) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.95.11.6436 · Cited by 333 · see citing works
Bacterial flagella explore microscale hummocks and hollows to increase adhesion
Friedlander, R. S., Vlamakis, H. C., Kim, P., Khan, M., Kolter, R., & Aizenberg, J. (2013) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1219662110 · Cited by 309 · see citing works
Dynamics of mechanosensing in the bacterial flagellar motor
Lele, P. P., Hosu, B. G., & Berg, H. C. (2013) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1305885110 · Cited by 308 · see citing works
A Molecular Clutch Disables Flagella in the Bacillus subtilis Biofilm
Blair, K. M., Turner, L., Winkelman, J. T., Berg, H. C., & Kearns, D. B. (2008) · Science · doi:10.1126/science.1157877 · Cited by 300 · see citing works
BslA is a self-assembling bacterial hydrophobin that coats the Bacillus subtilis biofilm
Hobley, L., Ostrowski, A., Rao, F. V., Bromley, K. M., Porter, M., Prescott, A. R., MacPhee, C. E., van Aalten, D. M. F., & Stanley‐Wall, N. R. (2013) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1306390110 · Cited by 292 · see citing works
Self-citation
Genes governing swarming in Bacillus subtilis and evidence for a phase variation mechanism controlling surface motility
Kearns, D. B., Chu, F., Rudner, R., & Losick, R. M. (2004) · Molecular Microbiology · doi:10.1111/j.1365-2958.2004.03996.x · Cited by 289 · see citing works
Flagellar dynamometer controls swarmer cell differentiation of V. parahaemolyticus
McCarter, L. L., Hilmen, M., & Silverman, M. R. (1988) · Cell · doi:10.1016/0092-8674(88)90197-3 · Cited by 278 · see citing works
BslA(YuaB) forms a hydrophobic layer on the surface of Bacillus subtilis biofilms
Kobayashi, K., & Iwano, M. (2012) · Molecular Microbiology · doi:10.1111/j.1365-2958.2012.08094.x · Cited by 276 · see citing works
Defining the genetic differences between wild and domestic strains of Bacillus subtilis that affect poly‐γ‐ dl ‐glutamic acid production and biofilm formation
Stanley‐Wall, N. R., & Lazazzera, B. A. (2005) · Molecular Microbiology · doi:10.1111/j.1365-2958.2005.04746.x · Cited by 259 · see citing works
Self-citation
Subcellular location characteristics of the Pseudomonas aeruginosa GGDEF protein, WspR, indicate that it produces cyclic‐di‐GMP in response to growth on surfaces
Güvener, Z. T., & Harwood, C. S. (2007) · Molecular Microbiology · doi:10.1111/j.1365-2958.2007.06008.x · Cited by 251 · see citing works
Gradual activation of the response regulator DegU controls serial expression of genes for flagellum formation and biofilm formation in Bacillus subtilis
Kobayashi, K. (2007) · Molecular Microbiology · doi:10.1111/j.1365-2958.2007.05923.x · Cited by 247 · see citing works
High-Precision, Whole-Genome Sequencing of Laboratory Strains Facilitates Genetic Studies
Srivatsan, A., Han, Y., Peng, J., Tehranchi, A. K., Gibbs, R. A., Wang, J. D., & Chen, R. (2008) · PLoS Genetics · doi:10.1371/journal.pgen.1000139 · Cited by 233 · see citing works
DegU co‐ordinates multicellular behaviour exhibited by Bacillus subtilis
Verhamme, D. T., Kiley, T. B., & Stanley‐Wall, N. R. (2007) · Molecular Microbiology · doi:10.1111/j.1365-2958.2007.05810.x · Cited by 230 · see citing works
Self-citation
Paracrine signaling in a bacteriumPreprint
López, D., Vlamakis, H. C., Losick, R. M., & Kolter, R. (2009) · Genes & Development · doi:10.1101/gad.1813709 · Cited by 228 · see citing works
Gene expression patterns during swarming in Salmonella typhimurium: genes specific to surface growth and putative new motility and pathogenicity genes
Wang, Q., Frye, J. G., McClelland, M., & Harshey, R. M. (2004) · Molecular Microbiology · doi:10.1111/j.1365-2958.2003.03977.x · Cited by 214 · see citing works
Function of Protonatable Residues in the Flagellar Motor of Escherichia coli : a Critical Role for Asp 32 of MotB
Zhou, J., Sharp, L. L., Tang, H. L., Lloyd, S. A., Billings, S. J., Braun, T. F., & Blair, D. F. (1998) · Journal of Bacteriology · doi:10.1128/jb.180.10.2729-2735.1998 · Cited by 213 · see citing works
Transient heterogeneity in extracellular protease production by Bacillus subtilis
Veening, J., Igoshin, O. A., Eijlander, R. T., Nijland, R., Hamoen, L. W., & Kuipers, O. P. (2008) · Molecular Systems Biology · doi:10.1038/msb.2008.18 · Cited by 205 · see citing works
Surface contact stimulates the just‐in‐time deployment of bacterial adhesins
Li, G., Brown, P., Tang, J. X., Xu, J., Quardokus, E. M., Fuqua, C., & Brun, Y. V. (2011) · Molecular Microbiology · doi:10.1111/j.1365-2958.2011.07909.x · Cited by 193 · see citing works
The proton flux through the bacterial flagellar motor
Meister, M., Lowe, G., & Berg, H. C. (1987) · Cell · doi:10.1016/0092-8674(87)90540-x · Cited by 191 · see citing works
Transcription initiation region of the srfA operon, which is controlled by the comP-comA signal transduction system in Bacillus subtilis
NAKANO, M. M., Xia, L., & Zuber, P. (1991) · Journal of Bacteriology · doi:10.1128/jb.173.17.5487-5493.1991 · Cited by 181 · see citing works
Sensing wetness: a new role for the bacterial flagellum
Wang, Q., Suzuki, A., Mariconda, S., Porwollik, S., & Harshey, R. M. (2005) · The EMBO Journal · doi:10.1038/sj.emboj.7600668 · Cited by 180 · see citing works
Stationary phase reorganisation of the Escherichia coli transcription machinery by Crl protein, a fine‐tuner of σs activity and levels
Typas, A., Barembruch, C., Possling, A., & Hengge, R. (2007) · The EMBO Journal · doi:10.1038/sj.emboj.7601629 · Cited by 179 · see citing works
Surface sensing in Vibrio parahaemolyticus triggers a programme of gene expression that promotes colonization and virulence
Gode‐Potratz, C. J., Kustusch, R. J., Breheny, P. J., Weiss, D. S., & McCarter, L. L. (2010) · Molecular Microbiology · doi:10.1111/j.1365-2958.2010.07445.x · Cited by 178 · see citing works
Solubilization and Purification of the MotA/MotB Complex of Escherichia coli
Kojima, S., & Blair, D. F. (2003) · Biochemistry · doi:10.1021/bi035405l · Cited by 170 · see citing works
The phosphorylation state of the DegU response regulator acts as a molecular switch allowing either degradative enzyme synthesis or expression of genetic competence in Bacillus subtilis.
Dahl, M. K., Msadek, T., Kunst, F., & Rapoport, G. (1992) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)49742-1 · Cited by 168 · see citing works
Surface sensing and lateral subcellular localization of WspA , the receptor in a chemosensory‐like system leading to c‐di‐ GMP production
O’Connor, J. R., Kuwada, N. J., Huangyutitham, V., Wiggins, P. A., & Harwood, C. S. (2012) · Molecular Microbiology · doi:10.1111/mmi.12013 · Cited by 166 · see citing works
DegS-DegU and ComP-ComA modulator-effector pairs control expression of the Bacillus subtilis pleiotropic regulatory gene degQ
Msadek, T., Kunst, F., Klier, A. F., & Rapoport, G. (1991) · Journal of Bacteriology · doi:10.1128/jb.173.7.2366-2377.1991 · Cited by 162 · see citing works
The sodium‐driven polar flagellar motor of marine Vibrio as the mechanosensor that regulates lateral flagellar expression
Kawagishi, I., Imagawa, M., Imae, Y., McCarter, L. L., & Homma, M. (1996) · Molecular Microbiology · doi:10.1111/j.1365-2958.1996.tb02509.x · Cited by 153 · see citing works
The cell biology of peritrichous flagella in B acillus subtilis
Guttenplan, S. B., Shaw, S. L., & Kearns, D. B. (2012) · Molecular Microbiology · doi:10.1111/mmi.12103 · Cited by 146 · see citing works
Evidence that acetyl phosphate functions as a global signal during biofilm development
Wolfe, A. J., Chang, D., Walker, J. D., Seitz‐Partridge, J. E., Vidaurri, M. D., Lange, C. F., Prüß, B. M., Henk, M. C., Larkin, J. C., & Conway, T. (2003) · Molecular Microbiology · doi:10.1046/j.1365-2958.2003.03457.x · Cited by 139 · see citing works
The EpsE Flagellar Clutch Is Bifunctional and Synergizes with EPS Biosynthesis to Promote Bacillus subtilis Biofilm Formation
Guttenplan, S. B., Blair, K. M., & Kearns, D. B. (2010) · PLoS Genetics · doi:10.1371/journal.pgen.1001243 · Cited by 134 · see citing works
Binding of response regulator DegU to the aprE promoter is inhibited by RapG, which is counteracted by extracellular PhrG in Bacillus subtilis
Ogura, M., Shimane, K., Asai, K., Ogasawara, N., & Tanaka, T. (2003) · Molecular Microbiology · doi:10.1046/j.1365-2958.2003.03665.x · Cited by 131 · see citing works
Biofilm formation by a Bacillus subtilis strain that produces γ-polyglutamate
Morikawa, M., Kagihiro, S., Haruki, M., Takano, K., Branda, S. S., Kolter, R., & Kanaya, S. (2006) · Microbiology · doi:10.1099/mic.0.29060-0 · Cited by 131 · see citing works
YuaB Functions Synergistically with the Exopolysaccharide and TasA Amyloid Fibers To Allow Biofilm Formation by Bacillus subtilis
Ostrowski, A., Mehert, A., Prescott, A. R., Kiley, T. B., & Stanley‐Wall, N. R. (2011) · Journal of Bacteriology · doi:10.1128/jb.00223-11 · Cited by 131 · see citing works
Self-citation
A pivotal role for the response regulator DegU in controlling multicellular behaviour
Murray, E. J., Kiley, T. B., & Stanley‐Wall, N. R. (2008) · Microbiology · doi:10.1099/mic.0.023903-0 · Cited by 130 · see citing works
Self-citation
Signal Integration by the Two-Component Signal Transduction Response Regulator CpxR
Wolfe, A. J., Parikh, N., Lima, B. P., & Zemaitaitis, B. W. (2008) · Journal of Bacteriology · doi:10.1128/jb.01906-07 · Cited by 129 · see citing works
The Ability ofProteus mirabilisTo Sense Surfaces and Regulate Virulence Gene Expression Involves FliL, a Flagellar Basal Body Protein
Belas, R., & Suvanasuthi, R. (2005) · Journal of Bacteriology · doi:10.1128/jb.187.19.6789-6803.2005 · Cited by 127 · see citing works
Coordinated Cyclic-Di-GMP Repression of Salmonella Motility through YcgR and Cellulose
Zorraquino, V., García, B., Latasa, C., Echeverz, M., Toledo‐Arana, A., Valle, J., Lasa, Í., & Solano, C. (2012) · Journal of Bacteriology · doi:10.1128/jb.01789-12 · Cited by 119 · see citing works
DegU and Spo0A Jointly Control Transcription of Two Loci Required for Complex Colony Development byBacillus subtilis
Verhamme, D. T., Murray, E. J., & Stanley‐Wall, N. R. (2008) · Journal of Bacteriology · doi:10.1128/jb.01236-08 · Cited by 110 · see citing works
Self-citation
Tryptophan-scanning mutagenesis of MotB, an integral membrane protein essential for flagellar rotation in Escherichia coli
Sharp, L. L., Zhou, J., & Blair, D. F. (1995) · Biochemistry · doi:10.1021/bi00028a028 · Cited by 108 · see citing works
Excretion of a protease by Serratia marcescens
Braun, V., & Schmitz, G. (1980) · Archives of Microbiology · doi:10.1007/bf00407028 · Cited by 105 · see citing works
Swarming motility and the control of master regulators of flagellar biosynthesis
Patrick, J. E., & Kearns, D. B. (2011) · Molecular Microbiology · doi:10.1111/j.1365-2958.2011.07917.x · Cited by 104 · see citing works
Extracellular signaling and multicellularity in Bacillus subtilis
Shank, E. A., & Kolter, R. (2011) · Current Opinion in Microbiology · doi:10.1016/j.mib.2011.09.016 · Cited by 103 · see citing works
Metabolic differentiation in actively swarming Salmonella
Kim, W., & Surette, M. G. (2004) · Molecular Microbiology · doi:10.1111/j.1365-2958.2004.04295.x · Cited by 93 · see citing works
Function of Proline Residues of MotA in Torque Generation by the Flagellar Motor of Escherichia coli
Braun, T. F., Poulson, S., Gully, J. B., Empey, J. C., Way, S. V., Putnam, A. R., & Blair, D. F. (1999) · Journal of Bacteriology · doi:10.1128/jb.181.11.3542-3551.1999 · Cited by 92 · see citing works
A Novel Factor Controlling Bistability in Bacillus subtilis: the YmdB Protein Affects Flagellin Expression and Biofilm Formation
Diethmaier, C., Pietack, N., Gunka, K., Wrede, C., Lehnik‐Habrink, M., Herzberg, C., Hübner, S., & Stülke, J. (2011) · Journal of Bacteriology · doi:10.1128/jb.05360-11 · Cited by 91 · see citing works
Mutational analysis of the Bacillus subtilis DegU regulator and its phosphorylation by the DegS protein kinase
Dahl, M. K., Msadek, T., Kunst, F., & Rapoport, G. (1991) · Journal of Bacteriology · doi:10.1128/jb.173.8.2539-2547.1991 · Cited by 90 · see citing works
A novel type of energetics in a marine alkali‐tolerant bacterium
Chernyak, B. V., Dibrov, P. A., Glagolev, A. N., Sherman, M. Y., & Skulachev, V. P. (1983) · FEBS Letters · doi:10.1016/0014-5793(83)80014-3 · Cited by 84 · see citing works
DegU-P Represses Expression of the Motilityfla-cheOperon inBacillus subtilis
Amati, G., Bisicchia, P., & Galizzi, A. (2004) · Journal of Bacteriology · doi:10.1128/jb.186.18.6003-6014.2004 · Cited by 84 · see citing works
Isolation and phosphorylation of the Bacillus subtilis degS and degU gene products.
Mukai, K., KAWATA, M., & Tanaka, T. (1990) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)45474-3 · Cited by 81 · see citing works
Bacillus subtilisResponse Regulator DegU Is a Direct Activator ofpgsBTranscription Involved in γ-Poly-glutamic Acid Synthesis
Ohsawa, T., Tsukahara, K., & Ogura, M. (2009) · Bioscience Biotechnology and Biochemistry · doi:10.1271/bbb.90341 · Cited by 81 · see citing works
A molecular brake, not a clutch, stops the Rhodobacter sphaeroides flagellar motor
Piližota, T., Brown, M. T., Leake, M. C., Branch, R. W., Berry, R. M., & Armitage, J. P. (2009) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0813164106 · Cited by 77 · see citing works
Osmotic pressure can regulate matrix gene expression in B acillus subtilis
Rubinstein, S. M., Kolodkin‐Gal, I., McLoon, A. L., Chai, L., Kolter, R., Losick, R. M., & Weitz, D. A. (2012) · Molecular Microbiology · doi:10.1111/j.1365-2958.2012.08201.x · Cited by 77 · see citing works
Evidence that metabolism and chromosome copy number control mutually exclusive cell fates in Bacillus subtilis
Chai, Y., Norman, T. M., Kolter, R., & Losick, R. M. (2011) · The EMBO Journal · doi:10.1038/emboj.2011.36 · Cited by 76 · see citing works
A Novel Gene Inactivation System Reveals Altered Periplasmic Flagellar Orientation in aBorrelia burgdorferi fliLMutant
Motaleb, M. A., Pitzer, J. E., Sultan, S. Z., & Liu, J. (2011) · Journal of Bacteriology · doi:10.1128/jb.00202-11 · Cited by 75 · see citing works
Whole-Genome Sequences of Bacillus subtilis and Close Relatives
Earl, A. M., Eppinger, M., Fricke, W. F., Rosovitz, M. J., Rasko, D. A., Daugherty, S., Losick, R. M., Kolter, R., & Ravel, J. (2012) · Journal of Bacteriology · doi:10.1128/jb.05675-11 · Cited by 67 · see citing works
More than Motility: Salmonella Flagella Contribute to Overriding Friction and Facilitating Colony Hydration during Swarming
Partridge, J. D., & Harshey, R. M. (2012) · Journal of Bacteriology · doi:10.1128/jb.02064-12 · Cited by 66 · see citing works
The ydaO motif is an ATP-sensing riboswitch in Bacillus subtilis
Watson, P. Y., & Fedor, M. J. (2012) · Nature Chemical Biology · doi:10.1038/nchembio.1095 · Cited by 65 · see citing works
The bacterial condensin/cohesin‐like protein complex acts in DNA repair and regulation of gene expression
Dervyn, E., Noirot‐Gros, M., Mervelet, P., McGovern, S., Ehrlich, S. D., Polard, P., & Noirot, P. H. (2004) · Molecular Microbiology · doi:10.1111/j.1365-2958.2003.03951.x · Cited by 60 · see citing works
Perturbation of FliL Interferes with Proteus mirabilis Swarmer Cell Gene Expression and Differentiation
Cusick, K. D., Lee, Y., Youchak, B., & Belas, R. (2011) · Journal of Bacteriology · doi:10.1128/jb.05998-11 · Cited by 56 · see citing works
DegU‐phosphate activates expression of the anti‐sigma factor FlgM in Bacillus subtilis
Hsueh, Y., Cozy, L. M., Sham, L., Calvo, R. A., Gutu, A. D., Winkler, M. E., & Kearns, D. B. (2011) · Molecular Microbiology · doi:10.1111/j.1365-2958.2011.07755.x · Cited by 51 · see citing works
The Flagellar Protein FliL Is Essential for Swimming in Rhodobacter sphaeroides
Suaste-Olmos, F., Domenzain, C., Mireles-Rodríguez, J. C., Poggio, S., Osorio, A. V., Dreyfus, G., & Camarena, L. (2010) · Journal of Bacteriology · doi:10.1128/jb.00655-10 · Cited by 49 · see citing works
Autoregulation of the Bacillus subtilis response regulator gene degU is coupled with the proteolysis of DegU‐P by ClpCP
Ogura, M., & Tsukahara, K. (2010) · Molecular Microbiology · doi:10.1111/j.1365-2958.2010.07047.x · Cited by 47 · see citing works
Post-translational control of Bacillus subtilis biofilm formation mediated by tyrosine phosphorylation
Kiley, T. B., & Stanley‐Wall, N. R. (2010) · Molecular Microbiology · doi:10.1111/j.1365-2958.2010.07382.x · Cited by 47 · see citing works
Self-citation
SwrAA activates poly-γ-glutamate synthesis in addition to swarming in Bacillus subtilis
Osera, C., Amati, G., Calvio, C., & Galizzi, A. (2009) · Microbiology · doi:10.1099/mic.0.026435-0 · Cited by 46 · see citing works
Mutations Suppressing the Loss of DegQ Function in Bacillus subtilis (natto) Poly-γ-Glutamate Synthesis
Huyen, T., Suzuki, Y., Abe, N., Kaneko, J., Itoh, Y., & Kimura, K. (2011) · Applied and Environmental Microbiology · doi:10.1128/aem.05827-11 · Cited by 45 · see citing works
Autoregulation ofswrAAand Motility inBacillus subtilis
Calvio, C., Osera, C., Amati, G., & Galizzi, A. (2008) · Journal of Bacteriology · doi:10.1128/jb.00455-08 · Cited by 41 · see citing works
Activity of Proteus mirabilis FliL Is Viscosity Dependent and Requires Extragenic DNA
Lee, Y.-Y., Patellis, J., & Belas, R. (2012) · Journal of Bacteriology · doi:10.1128/jb.02024-12 · Cited by 33 · see citing works
In Bacillus subtilis DegU-P Is a Positive Regulator of the Osmotic Response
Ruzal, S. M., & Sánchez-Rivas, C. (1998) · Current Microbiology · doi:10.1007/s002849900395 · Cited by 28 · see citing works
A functional Spo0A is required for maximal aprE expression in Bacillus subtilis
Olmos, J., Bolaños, V. M., Causey, S. C., Ferrari, E., Bollvar, F., & Valle, F. (1996) · FEBS Letters · doi:10.1016/0014-5793(96)00070-1 · Cited by 21 · see citing works

Flagged first, then by citations

Sorted by citations

The gut microbiome in atherosclerotic cardiovascular disease
Jie, Z., Xia, H., Zhong, S.-L., Feng, Q., Li, S., Liang, S., Zhong, H., Liu, Z., Gao, Y., Zhao, H., et al. (2017) · Nature Communications · doi:10.1038/s41467-017-00900-1 · Cited by 1,638 · see citing works
Biofilms, flagella, and mechanosensing of surfaces by bacteria
Belas, R. (2014) · Trends in Microbiology · doi:10.1016/j.tim.2014.05.002 · Cited by 388 · see citing works
Mechanomicrobiology: how bacteria sense and respond to forces
Dufrêne, Y. F., & Persat, A. (2020) · Nature Reviews Microbiology · doi:10.1038/s41579-019-0314-2 · Cited by 294 · see citing works
Type III secretion systems: the bacterial flagellum and the injectisome
Diepold, A., & Armitage, J. P. (2015) · Philosophical Transactions of the Royal Society B Biological Sciences · doi:10.1098/rstb.2015.0020 · Cited by 242 · see citing works
Biofilm formation by B acillus subtilis: new insights into regulatory strategies and assembly mechanisms
Cairns, L. S., Hobley, L., & Stanley‐Wall, N. R. (2014) · Molecular Microbiology · doi:10.1111/mmi.12697 · Cited by 234 · see citing works
Self-citation
Chemotaxis towards autoinducer 2 mediates autoaggregation in Escherichia coli
Laganenka, L., Colin, R., & Sourjik, V. (2016) · Nature Communications · doi:10.1038/ncomms12984 · Cited by 218 · see citing works
Sensational biofilms: surface sensing in bacteria
O’Toole, G. A., & Wong, G. C. L. (2016) · Current Opinion in Microbiology · doi:10.1016/j.mib.2016.02.004 · Cited by 188 · see citing works
Molecular mechanisms involved in B acillus subtilis biofilm formation
Mielich‐Süss, B., & López, D. (2014) · Environmental Microbiology · doi:10.1111/1462-2920.12527 · Cited by 187 · see citing works
How bacteria recognise and respond to surface contact
Kimkes, T. E. P., & Heinemann, M. (2019) · FEMS Microbiology Reviews · doi:10.1093/femsre/fuz029 · Cited by 174 · see citing works
The Structure and Regulation of Flagella in Bacillus subtilis
Mukherjee, S., & Kearns, D. B. (2014) · Annual Review of Genetics · doi:10.1146/annurev-genet-120213-092406 · Cited by 173 · see citing works
Dynamic cell–matrix interactions modulate microbial biofilm and tissue 3D microenvironments
Koo, H., & Yamada, K. M. (2016) · Current Opinion in Cell Biology · doi:10.1016/j.ceb.2016.05.005 · Cited by 141 · see citing works
Against the tide: the role of bacterial adhesion in host colonization
Stones, D. H., & Krachler, A. M. (2016) · Biochemical Society Transactions · doi:10.1042/bst20160186 · Cited by 136 · see citing works
Motility, Chemotaxis and Aerotaxis Contribute to Competitiveness during Bacterial Pellicle Biofilm Development
Hölscher, T., Bartels, B., Lin, Y.-C., Gallegos‐Monterrosa, R., Price‐Whelan, A., Kolter, R., Dietrich, L. E. P., & Kovács, Á. T. (2015) · Journal of Molecular Biology · doi:10.1016/j.jmb.2015.06.014 · Cited by 133 · see citing works
Host attachment and fluid shear are integrated into a mechanical signal regulating virulence in Escherichia coli O157:H7
Alsharif, G. S., Ahmad, S., Islam, M. S., Shah, R., Busby, S. J. W., & Krachler, A. M. (2015) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1422986112 · Cited by 122 · see citing works
Bacterial mechanosensing: the force will be with you, always
Gordon, V. D., & Wang, L. (2019) · Journal of Cell Science · doi:10.1242/jcs.227694 · Cited by 121 · see citing works
Cross-regulation of Pseudomonas motility systems: the intimate relationship between flagella, pili and virulence
Kazmierczak, B. I., Schniederberend, M., & Jain, R. (2015) · Current Opinion in Microbiology · doi:10.1016/j.mib.2015.07.017 · Cited by 120 · see citing works
Surface Sensing and Adaptation in Bacteria
Laventie, B., & Jenal, U. (2020) · Annual Review of Microbiology · doi:10.1146/annurev-micro-012120-063427 · Cited by 116 · see citing works
The Matrix Reloaded: How Sensing the Extracellular Matrix Synchronizes Bacterial Communities
Steinberg, N., & Kolodkin‐Gal, I. (2015) · Journal of Bacteriology · doi:10.1128/jb.02516-14 · Cited by 100 · see citing works
Adaptor-mediated Lon proteolysis restricts Bacillus subtilis hyperflagellation
Mukherjee, S., Bree, A. C., Liu, J., Patrick, J. E., Chien, P., & Kearns, D. B. (2014) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1417419112 · Cited by 95 · see citing works
Functional Regulators of Bacterial Flagella
Subramanian, S., & Kearns, D. B. (2019) · Annual Review of Microbiology · doi:10.1146/annurev-micro-020518-115725 · Cited by 91 · see citing works
Roadmap on emerging concepts in the physical biology of bacterial biofilms: from surface sensing to community formation
Wong, G. C. L., Antani, J. D., Lele, P. P., Chen, J., Nan, B., Kühn, M. J., Persat, A., Bru, J.-L., Høyland‐Kroghsbo, N. M., Siryaporn, A., et al. (2021) · Physical Biology · doi:10.1088/1478-3975/abdc0e · Cited by 90 · see citing works
Poly-γ-Glutamic Acids Contribute to Biofilm Formation and Plant Root Colonization in Selected Environmental Isolates of Bacillus subtilis
Yu, Y., Yan, F., Chen, Y., Jin, C. A., Guo, J.-H., & Chai, Y. (2016) · Frontiers in Microbiology · doi:10.3389/fmicb.2016.01811 · Cited by 88 · see citing works
Poly-γ-glutamic Acid Synthesis, Gene Regulation, Phylogenetic Relationships, and Role in Fermentation
Hsueh, Y., Huang, K., Kunene, S. C., & Lee, T.-Y. (2017) · International Journal of Molecular Sciences · doi:10.3390/ijms18122644 · Cited by 87 · see citing works
Trends in the application of Bacillus in fermented foods
Kimura, K., & Yokoyama, S. (2018) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2018.09.001 · Cited by 87 · see citing works
Modulation of flagellar rotation in surface-attached bacteria: A pathway for rapid surface-sensing after flagellar attachment
Schniederberend, M., Williams, J. F., Shine, E. E., Shen, C., Jain, R., Emonet, T., & Kazmierczak, B. I. (2019) · PLoS Pathogens · doi:10.1371/journal.ppat.1008149 · Cited by 87 · see citing works
Elevated exopolysaccharide levels in Pseudomonas aeruginosa flagellar mutants have implications for biofilm growth and chronic infections
Harrison, J. J., Almblad, H., Irie, Y., Wolter, D. J., Eggleston, H., Randall, T. E., Kitzman, J. O., Stackhouse, B., Emerson, J., McNamara, S., et al. (2020) · PLoS Genetics · doi:10.1371/journal.pgen.1008848 · Cited by 80 · see citing works
Advances in engineered Bacillus subtilis biofilms and spores, and their applications in bioremediation, biocatalysis, and biomaterials
Mohsin, M. Z., Omer, R., Huang, J., Mohsin, A., Guo, M., Qian, J., & Zhuang, Y. (2021) · Synthetic and Systems Biotechnology · doi:10.1016/j.synbio.2021.07.002 · Cited by 79 · see citing works
Regulation of Type IV Pili Contributes to Surface Behaviors of Historical and Epidemic Strains of Clostridium difficile
Purcell, E. B., McKee, R. W., Bordeleau, É., Burrus, V., & Tamayo, R. (2015) · Journal of Bacteriology · doi:10.1128/jb.00816-15 · Cited by 76 · see citing works
Bacterial mechanotransduction
Persat, A. (2017) · Current Opinion in Microbiology · doi:10.1016/j.mib.2016.12.002 · Cited by 76 · see citing works
Bacterial Adhesion on Soft Materials: Passive Physicochemical Interactions or Active Bacterial Mechanosensing?
Straub, H., Bigger, C. M., Valentin, J. D. P., Abt, D., Qin, X., Eberl, L., Maniura‐Weber, K., & Ren, Q. (2019) · Advanced Healthcare Materials · doi:10.1002/adhm.201801323 · Cited by 75 · see citing works
Cyclic diguanylate signaling in Gram-positive bacteria
Purcell, E. B., & Tamayo, R. (2016) · FEMS Microbiology Reviews · doi:10.1093/femsre/fuw013 · Cited by 75 · see citing works
Recent Advances in Surface Nanoengineering for Biofilm Prevention and Control. Part I: Molecular Basis of Biofilm Recalcitrance. Passive Anti-Biofouling Nanocoatings
Balaure, P. C., & Grumezescu, A. M. (2020) · Nanomaterials · doi:10.3390/nano10061230 · Cited by 73 · see citing works
Shelter in a Swarm
Harshey, R. M., & Partridge, J. D. (2015) · Journal of Molecular Biology · doi:10.1016/j.jmb.2015.07.025 · Cited by 72 · see citing works
Just in case it rains: building a hydrophobic biofilm the Bacillus subtilis way
Arnaouteli, S., MacPhee, C. E., & Stanley‐Wall, N. R. (2016) · Current Opinion in Microbiology · doi:10.1016/j.mib.2016.07.012 · Cited by 71 · see citing works
Self-citation
Phosphorylated DegU Manipulates Cell Fate Differentiation in the Bacillus subtilis Biofilm
Marlow, V. L., Porter, M., Hobley, L., Kiley, T. B., Swedlow, J. R., Davidson, F. A., & Stanley‐Wall, N. R. (2013) · Journal of Bacteriology · doi:10.1128/jb.00930-13 · Cited by 69 · see citing works
Self-citation
Reciprocal c-di-GMP signaling: Incomplete flagellum biogenesis triggers c-di-GMP signaling pathways that promote biofilm formation
Wu, D. C., Zamorano‐Sánchez, D., Pagliai, F. A., Park, J. H., Floyd, K. A., Lee, C. K., Kitts, G., Rose, C. B., Bilotta, E. M., Wong, G. C. L., et al. (2020) · PLoS Genetics · doi:10.1371/journal.pgen.1008703 · Cited by 67 · see citing works
Torque-dependent remodeling of the bacterial flagellar motor
Wadhwa, N., Phillips, R., & Berg, H. C. (2019) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1904577116 · Cited by 66 · see citing works
A Skeptic's Guide to Bacterial Mechanosensing
Chawla, R., Gupta, R., Lele, T. P., & Lele, P. P. (2019) · Journal of Molecular Biology · doi:10.1016/j.jmb.2019.09.004 · Cited by 65 · see citing works
The extracellular matrix protein TasA is a developmental cue that maintains a motile subpopulation within Bacillus subtilis biofilms
Steinberg, N., Keren‐Paz, A., Hou, Q., Doron, S., Yanuka‐Golub, K., Olender, T., Hadar, R., Rosenberg, G., Jain, R., Cámara-Almirón, J., et al. (2020) · Science Signaling · doi:10.1126/scisignal.aaw8905 · Cited by 63 · see citing works
A New Player at the Flagellar Motor: FliL Controls both Motor Output and Bias
Partridge, J. D., Nieto, V., & Harshey, R. M. (2015) · mBio · doi:10.1128/mbio.02367-14 · Cited by 61 · see citing works
Bacterial flagellar motility on hydrated rough surfaces controlled by aqueous film thickness and connectedness
Tecon, R., & Or, D. (2016) · Scientific Reports · doi:10.1038/srep19409 · Cited by 60 · see citing works
Intracellular Zn(II) Intoxication Leads to Dysregulation of the PerR Regulon Resulting in Heme Toxicity in Bacillus subtilis
Chandrangsu, P., & Helmann, J. D. (2016) · PLoS Genetics · doi:10.1371/journal.pgen.1006515 · Cited by 60 · see citing works
Bacterial biofilms as an essential component of rhizosphere plant-microbe interactions
Bhattacharyya, A., Mavrodi, O. V., Bhowmik, N., Weller, D. M., Thomashow, L. S., & Mavrodi, D. V. (2023) · Methods in microbiology · doi:10.1016/bs.mim.2023.05.006 · Cited by 58 · see citing works
Chemotaxis to self-generated AI-2 promotes biofilm formation in Escherichia coli
Jani, S., Seely, A. L., Peabody, G. L., V, Jayaraman, A., & Manson, M. D. (2017) · Microbiology · doi:10.1099/mic.0.000567 · Cited by 58 · see citing works
Defects in the Flagellar Motor Increase Synthesis of Poly-γ-Glutamate in Bacillus subtilis
Chan, J. M., Guttenplan, S. B., & Kearns, D. B. (2013) · Journal of Bacteriology · doi:10.1128/jb.01217-13 · Cited by 57 · see citing works
The prevalence and origin of exoprotease-producing cells in the Bacillus subtilis biofilm
Marlow, V. L., Cianfanelli, F. R., Porter, M., Cairns, L. S., Dale, J. K., & Stanley‐Wall, N. R. (2013) · Microbiology · doi:10.1099/mic.0.072389-0 · Cited by 57 · see citing works
Self-citation
Biophysical aspects underlying the swarm to biofilm transition
Worlitzer, V. M., Jose, A., Grinberg, I., Bär, M., Heidenreich, S., Eldar, A., Ariel, G., & Be’er, A. (2022) · Science Advances · doi:10.1126/sciadv.abn8152 · Cited by 50 · see citing works
Clostridium difficile Biofilm
Vuotto, C., Donelli, G., Buckley, A. M., & Chilton, C. H. (2018) · Advances in experimental medicine and biology · doi:10.1007/978-3-319-72799-8_7 · Cited by 49 · see citing works
Bacterial Tropone Natural Products and Derivatives: Overview of their Biosynthesis, Bioactivities, Ecological Role and Biotechnological Potential
Duan, Y., Petzold, M., Saleem-Batcha, R., & Teufel, R. (2020) · ChemBioChem · doi:10.1002/cbic.201900786 · Cited by 48 · see citing works
Whole-Genome Sequencing and Comparative Genome Analysis of Bacillus subtilis Strains Isolated from Non-Salted Fermented Soybean Foods
Kamada, M., Hase, S., Fujii, K., Miyake, M., Sato, K., Kimura, K., & Sakakibara, Y. (2015) · PLoS ONE · doi:10.1371/journal.pone.0141369 · Cited by 47 · see citing works
Physics of swimming and its fitness cost determine strategies of bacterial investment in flagellar motility
Lisevich, I., Colin, R., Yang, H. Y., Ni, B., & Sourjik, V. (2025) · Nature Communications · doi:10.1038/s41467-025-56980-x · Cited by 42 · see citing works
The Role of SwrA, DegU and PD3 in fla/che Expression in B. subtilis
Mordini, S., Osera, C., Marini, S., Scavone, F., Bellazzi, R., Galizzi, A., & Calvio, C. (2013) · PLoS ONE · doi:10.1371/journal.pone.0085065 · Cited by 42 · see citing works
Bacteria differently deploy type-IV pili on surfaces to adapt to nutrient availability
Ni, L., Yang, S., Zhang, R., Jin, Z., Chen, H., Conrad, J. C., & Jin, F. (2016) · npj Biofilms and Microbiomes · doi:10.1038/npjbiofilms.2015.29 · Cited by 42 · see citing works
Surface Engineering Strategies to Enhance the In Situ Performance of Medical Devices Including Atomic Scale Engineering
Sultana, A., Zare, M., Luo, H., & Ramakrishna, S. (2021) · International Journal of Molecular Sciences · doi:10.3390/ijms222111788 · Cited by 40 · see citing works
Flagellum-Mediated Mechanosensing and RflP Control Motility State of Pathogenic Escherichia coli
Laganenka, L., López, M. E., Colin, R., & Sourjik, V. (2020) · mBio · doi:10.1128/mbio.02269-19 · Cited by 40 · see citing works
When the swimming gets tough, the tough form a biofilm
Belas, R. (2013) · Molecular Microbiology · doi:10.1111/mmi.12354 · Cited by 40 · see citing works
Management of Osmotic Stress byBacillus Subtilis: Genetics and Physiology
Hoffmann, T., & Bremer, E. (2016) · Stress and Environmental Regulation of Gene Expression and Adaptation in Bacteria · doi:10.1002/9781119004813.ch63 · Cited by 39 · see citing works
Novel antibiofilm chemotherapies target nitrogen from glutamate and glutamine
Hassanov, T., Karunker, I., Steinberg, N., Erez, A., & Kolodkin‐Gal, I. (2018) · Scientific Reports · doi:10.1038/s41598-018-25401-z · Cited by 39 · see citing works
Cell biomechanics and mechanobiology in bacteria: Challenges and opportunities
Harper, C. E., & Hernandez, C. J. (2020) · APL Bioengineering · doi:10.1063/1.5135585 · Cited by 38 · see citing works
Mechanosensing: A Regulation Sensation
Ellison, C. K., & Brun, Y. V. (2015) · Current Biology · doi:10.1016/j.cub.2014.12.026 · Cited by 37 · see citing works
Loss of FliL Alters Proteus mirabilis Surface Sensing and Temperature-Dependent Swarming
Lee, Y., & Belas, R. (2014) · Journal of Bacteriology · doi:10.1128/jb.02235-14 · Cited by 37 · see citing works
Impact of high salinity and the compatible solute glycine betaine on gene expression of Bacillus subtilis
Rath, H., Sappa, P. K., Hoffmann, T., Salazar, M. G., Reder, A., Steil, L., Hecker, M., Bremer, E., Mäder, U., & Völker, U. (2020) · Environmental Microbiology · doi:10.1111/1462-2920.15087 · Cited by 34 · see citing works
Recent Advances and Future Prospects in Bacterial and Archaeal Locomotion and Signal Transduction
Bardy, S. L., Briegel, A., Rainville, S., & Krell, T. (2017) · Journal of Bacteriology · doi:10.1128/jb.00203-17 · Cited by 32 · see citing works
Dichotomous Role of Humic Substances in Modulating Transformation of Antibiotic Resistance Genes in Mineral Systems
Huang, Q., Zhu, J., Qu, C., Wang, Y., Hao, X., Chen, W., Cai, P., & Huang, Q. (2022) · Environmental Science & Technology · doi:10.1021/acs.est.2c06410 · Cited by 28 · see citing works
SwrD (YlzI) Promotes Swarming in Bacillus subtilis by Increasing Power to Flagellar Motors
Hall, A. N., Subramanian, S., Oshiro, R. T., Canzoneri, A. K., & Kearns, D. B. (2017) · Journal of Bacteriology · doi:10.1128/jb.00529-17 · Cited by 28 · see citing works
The S-Layer Homology Domain-Containing Protein SlhA from Paenibacillus alvei CCM 2051T Is Important for Swarming and Biofilm Formation
Janesch, B., Koerdt, A., Messner, P., & Schäffer, C. (2013) · PLoS ONE · doi:10.1371/journal.pone.0076566 · Cited by 27 · see citing works
Surface-associated microbes continue to surprise us in their sophisticated strategies for assembling biofilm communities
Wozniak, D. J., & Parsek, M. R. (2014) · F1000Prime Reports · doi:10.12703/p6-26 · Cited by 26 · see citing works
An alternate route to phosphorylating DegU of Bacillus subtilis using acetyl phosphate
Cairns, L. S., Martyn, J. E., Bromley, K. M., & Stanley‐Wall, N. R. (2015) · BMC Microbiology · doi:10.1186/s12866-015-0410-z · Cited by 26 · see citing works
Self-citation
Regulation of the Response Regulator Gene degU through the Binding of SinR/SlrR and Exclusion of SinR/SlrR by DegU in Bacillus subtilis
Ogura, M., Yoshikawa, H., & Chibazakura, T. (2013) · Journal of Bacteriology · doi:10.1128/jb.01321-13 · Cited by 24 · see citing works
A Flagella Hook Coding Gene flgE Positively Affects Biofilm Formation and Cereulide Production in Emetic Bacillus cereus
Li, Y., Chen, N., Wu, Q., Liang, X., Yuan, X., Zhu, Z., Zheng, Y., Yu, S., Chen, M., Zhang, J., et al. (2022) · Frontiers in Microbiology · doi:10.3389/fmicb.2022.897836 · Cited by 23 · see citing works
Computational Analysis of Host–Pathogen Protein Interactions between Humans and Different Strains of Enterohemorrhagic Escherichia coli
Bose, T., Venkatesh, K. V., & Mande, S. S. (2017) · Frontiers in Cellular and Infection Microbiology · doi:10.3389/fcimb.2017.00128 · Cited by 22 · see citing works
Mechanotransduction in Prokaryotes: A Possible Mechanism of Spaceflight Adaptation
Fajardo-Cavazos, P., & Nicholson, W. L. (2021) · Life · doi:10.3390/life11010033 · Cited by 22 · see citing works
FlgN Is Required for Flagellum-Based Motility by Bacillus subtilis
Cairns, L. S., Marlow, V. L., Kiley, T. B., Birchall, C., Ostrowski, A., Aldridge, P. D., & Stanley‐Wall, N. R. (2014) · Journal of Bacteriology · doi:10.1128/jb.01599-14 · Cited by 22 · see citing works
Self-citation
Mechanical stimuli activate gene expression via a cell envelope stress sensing pathway
Harper, C. E., Zhang, W., Lee, J., Shin, J., Keller, M. R., van Wijngaarden, E. W., Chou, E., Wang, Z., Dörr, T., Chen, P., et al. (2023) · Scientific Reports · doi:10.1038/s41598-023-40897-w · Cited by 21 · see citing works
SwrA as global modulator of the two-component system DegSU in Bacillus subtilis
Ermoli, F., Bontà, V., Vitali, G., & Calvio, C. (2021) · Research in Microbiology · doi:10.1016/j.resmic.2021.103877 · Cited by 21 · see citing works
Deletion of Rap‐Phr systems in Bacillus subtilis influences in vitro biofilm formation and plant root colonization
Nordgaard, M., Mortensen, R. M. R., Kirk, N. K., Gallegos‐Monterrosa, R., & Kovács, Á. T. (2021) · MicrobiologyOpen · doi:10.1002/mbo3.1212 · Cited by 19 · see citing works
Viscous drag on the flagellum activates Bacillus subtilis entry into the K‐state
Diethmaier, C., Chawla, R., Canzoneri, A. K., Kearns, D. B., Lele, P. P., & Dubnau, D. A. (2017) · Molecular Microbiology · doi:10.1111/mmi.13770 · Cited by 19 · see citing works
Identification of Genes Required for Swarming Motility in Bacillus subtilis Using Transposon Mutagenesis and High-Throughput Sequencing (TnSeq)
Sánchez, S., Snider, E. V., Wang, X., & Kearns, D. B. (2022) · Journal of Bacteriology · doi:10.1128/jb.00089-22 · Cited by 18 · see citing works
Identification of two novel highly inducible promoters from Bacillus licheniformis by screening transcriptomic data
Yuan, F., Li, K. V., Zhou, C., Zhou, H., Liu, H., Chai, H., Fuping, L., & Zhang, H. (2019) · Genomics · doi:10.1016/j.ygeno.2019.10.021 · Cited by 18 · see citing works
Binding of Phage-Encoded FlaGrab to Motile Campylobacter jejuni Flagella Inhibits Growth, Downregulates Energy Metabolism, and Requires Specific Flagellar Glycans
Sacher, J., Shajahan, A., Butcher, J., Patry, R. T., Flint, A., Hendrixson, D. R., Stintzi, A. P., Azadi, P., & Szymanski, C. M. (2020) · Frontiers in Microbiology · doi:10.3389/fmicb.2020.00397 · Cited by 18 · see citing works
Bacterial flagellar motor as a multimodal biosensor
Krasnopeeva, E., Barboza‐Pérez, U. E., Rosko, J., Piližota, T., & Lo, C. (2020) · Methods · doi:10.1016/j.ymeth.2020.06.012 · Cited by 17 · see citing works
Indole Derivatives Maintain the Status Quo Between Beneficial Biofilms and Their Plant Hosts
Ganin, H., Kemper, N., Meir, S., Rogachev, I., Ely, S., Massalha, H., Mandabi, A., Shanzer, A., Keren‐Paz, A., Meijler, M. M., et al. (2019) · Molecular Plant-Microbe Interactions · doi:10.1094/mpmi-12-18-0327-r · Cited by 17 · see citing works
Integrated Proteomics and Lipidomics Reveal That the Swarming Motility of Paenibacillus polymyxa Is Characterized by Phospholipid Modification, Surfactant Deployment, and Flagellar Specialization Relative to Swimming Motility
Poudel, S., Giannone, R. J., Farmer, A. T., Campagna, S. R., Bible, A. N., Morrell‐Falvey, J. L., Elkins, J. G., & Hettich, R. L. (2019) · Frontiers in Microbiology · doi:10.3389/fmicb.2019.02594 · Cited by 16 · see citing works
Impaired competence in flagellar mutants of Bacillus subtilis is connected to the regulatory network governed by DegU
Hölscher, T., Schiklang, T., Dragoš, A., Dietel, A., Kost, C., & Kovács, Á. T. (2017) · Environmental Microbiology Reports · doi:10.1111/1758-2229.12601 · Cited by 16 · see citing works
The force awakens: The dark side of mechanosensing in bacterial pathogens
Mordue, J., O’Boyle, N., Gadegaard, N., & Roe, A. J. (2020) · Cellular Signalling · doi:10.1016/j.cellsig.2020.109867 · Cited by 15 · see citing works
Collective Vortex-Like Movement of Bacillus subtilis Facilitates the Generation of Floating Biofilms
Steinberg, N., Rosenberg, G., Keren‐Paz, A., & Kolodkin‐Gal, I. (2018) · Frontiers in Microbiology · doi:10.3389/fmicb.2018.00590 · Cited by 15 · see citing works
Digestive exophagy of biofilms by intestinal amoeba and its impact on stress tolerance and cytotoxicity
Zanditenas, E., Trebicz‐Geffen, M., Kolli, D., Domínguez-García, L., Farhi, E., Linde, L., Romero, D., Chapman, M. R., Kolodkin‐Gal, I., & Ankri, S. (2023) · npj Biofilms and Microbiomes · doi:10.1038/s41522-023-00444-x · Cited by 14 · see citing works
Applying the handicap principle to biofilms: condition‐dependent signalling in Bacillus subtilis microbial communities
Harris, K. D., & Kolodkin‐Gal, I. (2018) · Environmental Microbiology · doi:10.1111/1462-2920.14497 · Cited by 14 · see citing works
Dynamic exchange of two types of stator units in Bacillus subtilis flagellar motor in response to environmental changes
Terahara, N., Namba, K., & Minamino, T. (2020) · Computational and Structural Biotechnology Journal · doi:10.1016/j.csbj.2020.10.009 · Cited by 14 · see citing works
Density and temperature controlled fluid extraction in a bacterial biofilm is determined by poly-γ-glutamic acid production
Morris, R. J., Stevenson, D., Sukhodub, T., Stanley‐Wall, N. R., & MacPhee, C. E. (2022) · npj Biofilms and Microbiomes · doi:10.1038/s41522-022-00361-5 · Cited by 12 · see citing works
Self-citation
Mechanosensing regulates virulence inEscherichia coliO157:H7
Islam, M. S., & Krachler, A. M. (2016) · Gut Microbes · doi:10.1080/19490976.2015.1121365 · Cited by 12 · see citing works
New Bacterial Surface Display System Development and Application Based on Bacillus subtilis YuaB Biofilm Component as an Anchoring Motif
Kim, D., Kim, W., & Kim, J. (2021) · Biotechnology and Bioprocess Engineering · doi:10.1007/s12257-020-0397-7 · Cited by 12 · see citing works
The chaperone ClpC participates in sporulation, motility, biofilm, and toxin production of Clostridioides difficile
Queraltó, C., Ortega, C., Díaz-Yáñez, F., Inostroza, O., Espinoza, G., Álvarez, R., González, R., Parra, F., Paredes‐Sabja, D., Acuña, L. G., et al. (2023) · Journal of Global Antimicrobial Resistance · doi:10.1016/j.jgar.2023.05.004 · Cited by 11 · see citing works
Novel fatty acids-governed cannibalism in beneficial rhizosphere Bacillus enhances biofilm formation via a two-component system OmpS/R and toxin transporter
Huang, R., Li, Q., Wang, D., Feng, H., Zhang, N., Shao, J., Shen, Q., Xu, Z., & Zhang, R. (2023) · 中国科学通报：英文版 · doi:10.1016/j.scib.2023.06.022 · Cited by 11 · see citing works
Bacillus subtilis degSU operon is regulated by the ClpXP-Spx regulated proteolysis system
Shiwa, Y., Yoshikawa, H., Tanaka, T., & Ogura, M. (2014) · The Journal of Biochemistry · doi:10.1093/jb/mvu076 · Cited by 11 · see citing works
Citrate Supplementation Modulates Medium Viscosity and Poly‐γ‐Glutamic Acid Synthesis by Engineered B. subtilis 168
Völker, F., Hoffmann, K., Halmschlag, B., Maaß, S., Büchs, J., & Blank, L. M. (2025) · Engineering in Life Sciences · doi:10.1002/elsc.70009 · Cited by 9 · see citing works
Viscosity-dependent determinants of Campylobacter jejuni impacting the velocity of flagellar motility
Ribardo, D. A., Johnson, J. G., & Hendrixson, D. R. (2023) · mBio · doi:10.1128/mbio.02544-23 · Cited by 9 · see citing works
Flagellar rotation facilitates the transfer of a bacterial conjugative plasmid
Bhattacharya, S., Bejerano‐Sagie, M., Ravins, M., Zeroni, L., Kaur, P., Gopu, V., Rosenshine, I., & Ben‐Yehuda, S. (2024) · The EMBO Journal · doi:10.1038/s44318-024-00320-0 · Cited by 7 · see citing works
More than a Feeling: Microscopy Approaches to Understanding Surface-Sensing Mechanisms
Graham, K. J., & Burrows, L. L. (2020) · Journal of Bacteriology · doi:10.1128/jb.00492-20 · Cited by 7 · see citing works
Biofilms: Besieged Cities or Thriving Ports?
Kroukamp, O., Bester, E., & Wolfaardt, G. M. (2019) · Advances in environmental microbiology · doi:10.1007/978-3-030-16775-2_3 · Cited by 7 · see citing works

When the swimming gets tough, the tough form a biofilm
Belas, R. (2013) · Molecular Microbiology · doi:10.1111/mmi.12354 · Cited by 40 · see citing works
Flagella function as mechanosensors
Kåhrström, C. T. (2013) · Nature Reviews Microbiology · doi:10.1038/nrmicro3102
Gradual activation of the response regulator DegU controls serial expression of genes for flagellum formation and biofilm formation in Bacillus subtilis
Kobayashi, K. (2007) · Molecular Microbiology · doi:10.1111/j.1365-2958.2007.05923.x · Cited by 247 · see citing works
Biofilms, flagella, and mechanosensing of surfaces by bacteria
Belas, R. (2014) · Trends in Microbiology · doi:10.1016/j.tim.2014.05.002 · Cited by 388 · see citing works
DegU‐phosphate activates expression of the anti‐sigma factor FlgM in Bacillus subtilis
Hsueh, Y., Cozy, L. M., Sham, L., Calvo, R. A., Gutu, A. D., Winkler, M. E., & Kearns, D. B. (2011) · Molecular Microbiology · doi:10.1111/j.1365-2958.2011.07755.x · Cited by 51 · see citing works

Authors: Cairns, L. S., Marlow, V. L., Bissett, E., Ostrowski, A., & Stanley‐Wall, N. R.
Affiliations: University of Dundee (GB)
Corresponding author: Stanley‐Wall, Nicola R.
ORCID: Cairns, Lynne S.
Ostrowski, Adam
Stanley‐Wall, Nicola R.
Venue: Molecular Microbiology
Publisher: Wiley
Published: 2013-08-14
Volume: 90
Issue: 1
Pages: 6-21
ISSN: 0950-382X (ISSN-L)
1365-2958
DOI: 10.1111/mmi.12342
PMC: PMC3963450
OpenAlex: W1908456294
Cited by: 143
References: 98
Citations by year: 2026: 7 · 2025: 8 · 2024: 7 · 2023: 7 · 2022: 9 · 2021: 13 · 2020: 17 · 2019: 12 · 2018: 6 · 2017: 12 · 2016: 13 · 2015: 13 · 2014: 11 · 2013: 8
FWCI: 9.95
Type: journal article
Language: en
Open access: hybrid
doi.org
Licence: cc-by
creativecommons.org/licenses/by/3.0/ (vor)
Locations: Molecular Microbiology (publishedVersion, cc-by)
Europe PMC (PubMed Central) (publishedVersion, cc-by)
PubMed Central (publishedVersion, cc-by)
PubMed (publishedVersion)
Discovery Research Portal (University of Dundee) (submittedVersion)
Funders: Wellcome Trust (093714, 097945, 093714/Z/10/Z, 097818/Z/11, 097945/B/11/Z, 097818/Z/11/A)
International Seafood Sustainability Foundation
Directorate for Biological Sciences
Biotechnology and Biological Sciences Research Council (BB/I006915/1, BB/H002340/1, BB/D526161/1)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bacterial biofilms and quorum sensing
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Biochemical and Structural Characterization
Keywords: Bacillus subtilis, flagella, mechanosensing, biofilm formation, MotB, bacterial signal transduction
MeSH terms: Bacillus subtilis, Bacterial Proteins, Flagella, Locomotion, Signal Transduction, Gene Expression Regulation, Bacterial

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

A mechanical signal transmitted by the flagellum controls signalling in B acillus subtilis

Abstract

Integrity signals

Claims the paper makes

Claims other papers make about this one

Self-check

Cites

Cited by

Related

Record

About these checks