Vasoactive intestinal polypeptide regulates barrier function via mast cells in human intestinal follicle-associated epithelium and during stress in rats
Abstract
BACKGROUND: Vasoactive intestinal polypeptide (VIP) has been implicated as a regulator of intestinal barrier function and inflammation. Our aim was to elucidate the role of VIP in follicle-associated epithelium (FAE) and villus epithelium (VE) permeability following stress in rats and on human intestinal barrier function. METHODS: Rats were injected intraperitoneally (i.p.) with VIP receptor-antagonists (anti-VPACs), a mast cell stabilizer, doxantrazole (DOX), or NaCl, and submitted to acute water avoidance stress. Ileal segments were mounted in Ussing chambers to assess (51) chromium-edta ((51) Cr-edta) and Escherichia (E.) coli (strain K-12) permeability. Rat ileal and human ileal and colonic segments were exposed to VIP ± anti-VPACs or DOX. An in vitro co-culture model of human FAE was used to study epithelial-VIP effects. VIP/VPACs distribution was assessed by microscopy. KEY RESULTS: Stress increased (51) Cr-edta and E. coli permeability in VE and FAE. The increases were abolished by i.p. injection of DOX or anti-VPACs. Ileal VIP-exposure ex vivo increased bacterial passage and this was reduced by DOX. In human FAE ex vivo, VIP treatment doubled bacterial uptake, which was normalized by DOX or anti-VPACs. No barrier effects were observed in human colonic tissue. VPACs were found in rat and human ileal follicles, with partial mast cell co-localization. The co-culture model confirmed VIP-mast cell-epithelial interactions in the regulation of barrier function. CONCLUSIONS & INFERENCES: Stress affects the FAE barrier by mechanisms involving VIP and VPACs on mucosal mast cells. We suggest a regulatory role for VIP in the control of ileal permeability that may be relevant to bacterial-epithelial interactions in stress-related intestinal disorders.
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VIP-bacterial-epithelial interactions regulate intestinal barrier function.
“These findings highlight an important effect of VIP-bacterial-epithelial interactions on regulating intestinal barrier function (39).”
From Stress triggers flare of inflammatory bowel disease in children and adults (2019) · cites this paper as [39]
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Stress triggers flare of inflammatory bowel disease in children and adults
Sun, Y., Li, L., Xie, R., Wang, B., Jiang, K., & Cao, H. (2019) · Frontiers in Pediatrics · doi:10.3389/fped.2019.00432 · Cited by 171 · see citing works
- Authors
- Keita, Å. V., Carlsson, A. H., Cigéhn, M., Ericson, A. ‐., McKay, D. M., & Söderholm, J. D.
- Affiliations
- Linköping University (SE)
- University of Calgary (CA)
- Corresponding author
- Söderholm, J. D.
- Venue
- Neurogastroenterology & Motility
- Publisher
- Wiley
- Published
- 2013-04-18
- Volume
- 25
- Issue
- 6
- Pages
- e406-17
- ISSN
- 1350-1925 (ISSN-L)
- 1365-2982
- OpenAlex
- W2007043162
- Cited by
- 53
- References
- 52
- Citations by year
- 2024: 4 · 2023: 5 · 2022: 5 · 2021: 6 · 2020: 8 · 2019: 7 · 2018: 5 · 2017: 4 · 2016: 2 · 2015: 1 · 2014: 4 · 2013: 2
- FWCI
- 1.47
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Neurogastroenterology & Motility (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuropeptides and Animal Physiology
- Health Sciences › Medicine › Gastroenterology › Gastrointestinal motility and disorders
- Life Sciences › Immunology and Microbiology › Immunology › Mast cells and histamine
- Keywords
- vasoactive intestinal peptide, intestinal barrier function, mast cells, follicle-associated epithelium, stress, VPAC receptors, doxantrazole, mast cell stabilizers, bacterial translocation, Escherichia coli, ileum, colonic mucosa
- MeSH terms
- Aged, Aged, 80 and over, Animals, Female, Humans, Ileum, Intestinal Mucosa, Male, Mast Cells, Middle Aged, Permeability, Phosphodiesterase Inhibitors, Stress, Physiological, Thioxanthenes, Vasoactive Intestinal Peptide, Rats, Wistar, Receptors, Vasoactive Intestinal Peptide, Xanthones, Rats
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