Assessing the colonic microbiome, hydrogenogenic and hydrogenotrophic genes, transit and breath methane in constipation
Open accessAbstract
Background: Differences in the gut microbiota and breath methane production have been observed in chronic constipation, but the relationship between colonic microbiota, transit, and breath tests remains unclear. Methods: In 25 healthy and 25 constipated females we evaluated the sigmoid colonic mucosal and fecal microbiota using 16S rRNA gene sequencing, abundance of hydrogenogenic FeFe (FeFe‐hydA) and hydrogenotrophic (methyl coenzyme M reductase A [mrcA] and dissimilatory sulfite reductase A [dsrA]) genes with real‐time qPCR assays, breath hydrogen and methane levels after oral lactulose, and colonic transit with scintigraphy. Key Results: Breath hydrogen and methane were not correlated with constipation, slow colon transit, or with abundance of corresponding genes. After adjusting for colonic transit, the abundance of FeFehydA, dsrA, and mcrA were greater (P<.005) in colonic mucosa, but not stool, of constipated patients. The abundance of the selected functional gene targets also correlated with that of selected taxa. The colonic mucosal abundance of FeFe‐hydA, but not mcrA, correlated positively (P<.05) with breath methane production, slow colonic transit, and overall microbiome composition. In the colonic mucosa and feces, the abundance of hydrogenogenic and hydrogenotrophic genes were positively correlated (P<.05). Breath methane production was not associated with constipation or colonic transit. Conclusions & Inferences: Corroborating our earlier findings with 16S rRNA genes, colonic mucosal but not fecal hydrogenogenic and hydrogenotrophic genes were more abundant in constipated vs. healthy subjects independent of colonic transit. Breath gases do not directly reflect the abundance of target genes contributing to their production.
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In women with and without constipation, qPCR of colonic mucosa found more hydrogen-producing and hydrogen-using genes in the constipated group.
“Analysis of functional gene targets in constipated and healthy females also has demonstrated increased abundance of hydrogenogenic (hydrogen producing) and hydrogenotrophic (hydrogen utilizing) genes by qPCR in colonic mucosa of constipated individuals163.”
From The gut Microbiome in adult and pediatric functional gastrointestinal disorders (2019) · cites this paper as [163]
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The gut Microbiome in adult and pediatric functional gastrointestinal disorders
Shin, A., Preidis, G. A., Shulman, R., & Kashyap, P. C. (2019) · Clinical Gastroenterology and Hepatology · doi:10.1016/j.cgh.2018.08.054 · Cited by 163 · see citing works
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- Authors
- Wolf, P. G., Parthasarathy, G., Chen, J., O'Connor, H. M., Chia, N., Bharucha, A. E., & Gaskins, H. R.
- Affiliations
- University of Illinois Urbana-Champaign (US)
- Mayo Clinic (US)
- Clinical Research and Trials Unit Center for Clinical and Translational Science Rochester MN USA
- Corresponding author
- Bharucha, A. E.
- Venue
- Neurogastroenterology & Motility
- Publisher
- Wiley
- Published
- 2017-03-13
- Volume
- 29
- Issue
- 10
- Pages
- 1-9
- ISSN
- 1350-1925 (ISSN-L)
- 1365-2982
- PMC
- PMC5593760
- OpenAlex
- W2614718459
- Cited by
- 26
- References
- 41
- Citations by year
- 2025: 2 · 2024: 1 · 2023: 1 · 2022: 6 · 2021: 4 · 2020: 4 · 2019: 3 · 2018: 4 · 2017: 1
- FWCI
- 3.44
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Neurogastroenterology & Motility (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Institutes of Health (R01 DK78924)
- National Center for Advancing Translational Sciences (UL1 TR000135)
- Topics
- Health Sciences › Medicine › Gastroenterology › Gastrointestinal motility and disorders
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Physical Sciences › Engineering › Building and Construction › Anaerobic Digestion and Biogas Production
- Keywords
- chronic constipation, colonic microbiota, breath methane, breath hydrogen, colonic transit, mcrA, DsrA, colonic mucosa, fecal microbiota, lactulose breath test, gut microbiota
- MeSH terms
- Gastrointestinal Microbiome, Adult, Breath Tests, Constipation, DNA, Bacterial, Female, Gastrointestinal Transit, Humans, Methane, Middle Aged
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