Lactobacilli postbiotics reduce biofilm formation and alter transcription of virulence genes of Aggregatibacter actinomycetemcomitans
Abstract
Abstract Periodontitis is characterized by a dysbiotic microbial community and treatment strategies include the reestablishment of symbiosis by reducing pathogens abundance. Aggregatibacter actinomycetemcomitans (Aa) is frequently associated with rapidly progressing periodontitis. Since the oral ecosystem may be affected by metabolic end‐products of bacteria, we evaluated the effect of soluble compounds released by probiotic lactobacilli, known as postbiotics, on Aa biofilm and expression of virulence‐associated genes. Cell‐free pH‐neutralized supernatants (CFS) of Lactobacillus rhamnosus Lr32, L . rhamnosus HN001, Lactobacillus acidophilus LA5, and L . acidophilus NCFM were tested against a fimbriated clinical isolate of Aa JP2 genotype (1 × 10 7 CFU/well) on biofilm formation for 24 hr, and early and mature preformed biofilms (2 and 24 hr). Lactobacilli CFS partially reduced Aa viable counts and biofilms biomass, but did not affect the number of viable non‐adherent bacteria, except for LA5 CFS. Furthermore, LA5 CFS and, in a lesser extent HN001 CFS, influenced Aa preformed biofilms. Lactobacilli postbiotics altered expression profile of Aa in a strain‐specific fashion. Transcription of cytolethal distending toxin ( cdtB ) and leukotoxin ( ltxA ) was downregulated by CFS of LA5 and LR32 CFS. Although all probiotics produced detectable peroxide, transcription of katA was downregulated by lactobacilli CFS. Transcription of dspB was abrogated by LR32 and NCFM CFS, but increased by HN001, whereas expression of pgA was not affected by any postbiotic. Our data indicated the potential of postbiotics from lactobacilli, especially LA5, to reduce colonization levels of Aa and to modulate the expression of virulence factors implicated in evasion of host defenses.
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Cell-free supernatants of Lactobacillus rhamnosus and Lactobacillus acidophilus can exert an antibiofilm effect by impairing biofilm integrity and reducing the expression of biofilm-associated genes of periodontal pathogens.
“Cell-free supernatants of Lactobacillus rhamnosus and Lactobacillus acidophilus can exert an antibiofilm effect by impairing biofilm integrity and reducing the expression of the biofilm-associated genes of periodontal pathogens [13,14].”
From Analysis of Chemical Structure and Antibiofilm Properties of Exopolysaccharides from Lactiplantibacillus plantarum EIR/IF-1 Postbiotics (2022) · cites this paper as [13]
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Outside the limits of bacterial viability: Postbiotics in the management of periodontitis
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“Cell-free supernatants of Lactobacillus rhamnosus and Lactobacillus acidophilus can exert an antibiofilm effect by impairing biofilm integrity and reducing the expression of the biofilm-associated genes of periodontal pathogens [13,14].”
Background
- Authors
- Ishikawa, K. H., Bueno, M. R., Kawamoto, D., Simionato, M. R. L., & Mayer, M. P. A.
- Affiliations
- Universidade de São Paulo (BR)
- Department of Microbiology Institute of Biomedical Sciences University of São Paulo São Paulo Brazil
- Venue
- Molecular Oral Microbiology
- Publisher
- Wiley
- Published
- 2021-01-17
- Volume
- 36
- Issue
- 1
- Pages
- 92-102
- ISSN
- 2041-1006 (ISSN-L)
- 2041-1014
- OpenAlex
- W3117603122
- Cited by
- 64
- References
- 75
- Citations by year
- 2026: 5 · 2025: 19 · 2024: 17 · 2023: 7 · 2022: 14 · 2021: 2
- FWCI
- 5.85
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Molecular Oral Microbiology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Fundação de Amparo à Pesquisa do Estado de São Paulo (2015/18273‐9, 2016/13156‐7, 2017/16377‐7)
- Topics
- Health Sciences › Dentistry › Periodontics › Oral microbiology and periodontitis research
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bacterial biofilms and quorum sensing
- Keywords
- Aggregatibacter actinomycetemcomitans, biofilm formation, virulence gene expression, periodontitis, Lactobacillus acidophilus LA-5, Lactobacillus rhamnosus, cell-free supernatant, cytolethal distending toxin, leukotoxin, kata, PGA, probiotics, oral dysbiosis
- MeSH terms
- Lactobacillus, Virulence, Aggregatibacter actinomycetemcomitans, Ecosystem, Biofilms, Probiotics
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