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Cited by 46Parasites and Host InteractionsInflammatory Bowel DiseaseIL-33, ST2, and ILC Pathways

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A recombinant cystatin from Ascaris lumbricoides attenuates inflammation of DSS‐induced colitis

Coronado, S., Barrios, L., Zakzuk, J., Regino, R., Ahumada, V., Franco, L., Ocampo, Y., Caraballo, L.·2017·Parasite Immunology
DOI 10.1111/pim.12425
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Abstract

Summary: Helminthiasis may ameliorate inflammatory diseases, such as inflammatory bowel disease and asthma. Information about immunomodulators from Ascaris lumbricoides is scarce, but could be important considering the co‐evolutionary relationships between helminths and humans. We evaluated the immunomodulatory effects of a recombinant cystatin from A. lumbricoides on an acute model of dextran sodium sulphate (DSS)‐induced colitis in mice. From an A. lumbricoides cDNA library, we obtained a recombinant cystatin (rA l‐CPI). Protease activity inhibition was demonstrated on cathepsin B and papain. Immunomodulatory effects were evaluated at two intraperitoneal doses (0.5 and 0.25 μg/G) on mice with DSS‐induced colitis. Body weight, colon length, Disease Activity Index (DAI), histological inflammation score, myeloperoxidase (MPO) activity, gene expression of cytokines and cytokines levels in colon tissue were analysed. Treatment with rA l‐CPI significantly reduced DAI, MPO activity and inflammation score without toxic effects. Also, IL‐10 and TGF‐B gene overexpression was observed in rA l‐CPI‐treated group compared to DSS‐exposed control and healthy mice. Furthermore, a reduction in IL‐6 and TNF‐A expression was found, and this was confirmed by the levels of these cytokines in colonic tissue. In conclusion, rA l‐CPI reduces inflammation in a mouse model of DSS‐induced colitis, probably by increasing the expression of anti‐inflammatory cytokines and reducing pro‐inflammatory ones.

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  • Helminth cystatins from S. japonicum, Ascaris lumbricoides, and Clonorchis sinensis all ameliorate colitis in vivo.

    “Other helminth cystatins with broad immunomodulatory properties including those from S. japonicum (Wang et al., 2016), Ascaris lumbricoides, and the liver fluke Clonorchis sinensis all ameliorate colitis in vivo”

    From Modulation of Host Immunity by Helminths: The Expanding Repertoire of Parasite Effector Molecules (2018) · cites this paper as Coronado et al., 2017

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Metadata

Authors
Coronado, S., Barrios, L., Zakzuk, J., Regino, R., Ahumada, V., Franco, L., Ocampo, Y., & Caraballo, L.
Affiliations
Universidad de Cartagena (CO)
Corresponding author
Caraballo, L.
ORCID
Venue
Parasite Immunology
Publisher
Wiley
Published
2017-04
Volume
39
Issue
4
ISSN
  • 0141-9838 (ISSN-L)
  • 1365-3024
DOI
10.1111/pim.12425
OpenAlex
W2593013667
Cited by
46
References
0
Citations by year
2026: 4 · 2025: 3 · 2024: 4 · 2023: 3 · 2022: 3 · 2021: 6 · 2020: 6 · 2019: 5 · 2018: 7 · 2017: 5
FWCI
5.45
Type
journal article
Language
en
Open access
closed
Licence
onlinelibrary.wiley.com/termsAndConditions#vor (vor, 5 d)
Locations
  • Parasite Immunology (publishedVersion)
  • PubMed (publishedVersion)
Funders
Departamento Administrativo de Ciencia, Tecnología e Innovación (406‐2011)
Topics
  • Life Sciences › Immunology and Microbiology › Parasitology › Parasites and Host Interactions
  • Health Sciences › Medicine › Gastroenterology › Inflammatory Bowel Disease
  • Life Sciences › Immunology and Microbiology › Immunology › IL-33, ST2, and ILC Pathways
Keywords
Ascaris lumbricoides, cystatin, recombinant protein, immunomodulation, DSS-induced colitis, inflammatory bowel disease, helminth immunomodulation, cathepsin B, papain, protease inhibition, IL-10, TGF-beta, interleukin-6, TNF-α, cytokine expression, myeloperoxidase, colonic inflammation
MeSH terms
Animals, Colitis, Colon, Disease Models, Animal, Female, Humans, Immunosuppressive Agents, Inflammation, Mice, Inbred BALB C, Recombinant Proteins, Cystatins, Cytokines, Dextran Sulfate, Ascaris lumbricoides, Mice

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