CoChat
Sign Up
Cited by 22Gestational Diabetes Research and ManagementMonoclonal and Polyclonal Antibodies ResearchBirth, Development, and Health

Maternal–Foetal Diabetes Modifies Neonatal Fc Receptor Expression on Human Leucocytes

Open access
de Souza, E. G., Hara, C. C. P., Fagundes, D. L. G., de Queiroz, A. A., Morceli, G., Calderon, I. M. P., França, E. L., Honorio‐França, A. C.·2016·Scandinavian Journal of Immunology
DOI 10.1111/sji.12466
Open this paper in CoChat — free accountOpen PDF

Abstract

This study investigated the expression of the neonatal Fc receptor (FcRn) in maternal blood, cord blood and placental cells and determined IgG levels in maternal blood and cord blood from diabetic mothers. Peripheral blood, cord blood and placenta samples were collected from 26 mothers with normoglycaemia (non‐diabetic, ND group) and 52 with hyperglycaemia (26 with mild gestational hyperglycaemia, MGH group, and 26 with type 2 diabetes mellitus, DM‐2 group). Cells expressing CD 19+ and FcRn were identified by flow cytometry. Total IgG and its subclasses were quantified by ELISA. Maternal blood from DM‐2 and cord blood from MGH exhibited a higher proportion of CD 19+ expression by B cells. DM‐2 showed a lower proportion of CD 19+ cells in placenta. FcRn expression increased in cells from cord blood and placenta from MGH. Maternal blood, cord blood and placenta cells from DM‐2 showed lower FcRn expression. Blood IgG levels were lower in DM‐2, and cord blood IgG levels were higher in MGH. The highest levels of IgG4 were detected in the blood of hyperglycaemic mothers. The highest IgG3 and IgG4 levels in cord blood were detected in MGH, and the lowest IgG2 and IgG3 levels in DM‐2. Maternal hyperglycaemia compromised placental transfer of IgG1, IgG3 and IgG4. The results suggest that regardless of hyperglycaemia degree, it decreases FcRn expression in placenta and blood cells and compromises the production and transfer of antibodies from maternal blood to newborns.

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1111/sji.12466

Cites

5 of 5 references verified0 citation errors · 0 not checkable

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • There was no statistically significant difference in total IgG levels in newborns between groups of mothers.

    “There was no statistically significant difference in total IgG levels in newborns between groups of mothers.”

    From Factors Affecting the FcRn-Mediated Transplacental Transfer of Antibodies and Implications for Vaccination in Pregnancy (2017) · cites this paper as [115]

    Table 2 reports a statistically significant difference in total IgG in cord blood between the mild gestational hyperglycaemia and non-diabetic groups (P < 0.05); the Results also describe higher cord-blood IgG in the former group. Although the Results say newborn IgG was similar between groups when discussing Figure 4, that figure presents placental transfer rates, not the cord-blood concentrations reported in Table 2. The claim that there was no significant difference in total IgG levels between groups is therefore contrary to the reported concentration results. (LLM, conf=0.84)

    Checked by a reasoning model — may be wrong.

    Experimental — accuracy not yet measured

  • FcRn expression increased with mild gestational hyperglycemia.

    “Interestingly however, FcRn expression increased with mild gestational hyperglycemia.”

    From Factors Affecting the FcRn-Mediated Transplacental Transfer of Antibodies and Implications for Vaccination in Pregnancy (2017) · cites this paper as [115]

  • On subclass analysis, there was significantly lower transfer of IgG1, IgG3, and IgG4 in women affected by diabetes, but significantly higher transfer of IgG3 in women with mild gestational hyperglycemia.

    “Differences were observed on subclass analysis however, with significantly lower transfer of IgG1, IgG3, and IgG4 in women affected by diabetes, but significantly higher transfer of IgG3 in women with mild gestational hyperglycemia.”

    From Factors Affecting the FcRn-Mediated Transplacental Transfer of Antibodies and Implications for Vaccination in Pregnancy (2017) · cites this paper as [115]

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Cites

Flagged first, then by citations

Research works printed but not linked: 35

  1. Graves DT, Kayal RA. Diabetic complications and dysregulated innate immunity. Front Biosci 2008;13:1227-39.
  2. Franc ßa EL, Morceli G, Fagundes DLG et al. Secretory IgA Fca receptor interaction modulating phagocytosis and microbicidal activity by phagocytes in human colostrum of diabetics. APMIS 2011;119:710-9.
  3. Morceli G, Franc ßa EL, Magalhães VB et al. Diabetes induced immunological and biochemical changes in human colostrum. Acta Paediatr 2011;100:550-6.
  4. Fagundes DLG, Franc ßa EL, Morceli G et al. The role of cytokines in the functional activity of phagocytes in blood and colostrum of diabetic mothers. Clin Develop Immunol 2013;590190:8. DOI: 10.1155/2013/590190. · DOI
  5. Morceli G, Honorio-Franc ßa AC, Fagundes DLG et al. Antioxidant effect of melatonin on the functional activity of colostral phagocytes in diabetic women. PLoS One 2013;8:e56915.
  6. Fagundes DLG, Franc ßa EL, Fernandes RTS et al. Changes in T-cell phenotype and cytokines profile in maternal blood, cord blood and colostrum of diabetic mothers. J Matern Fetal Neonatal Med 2016;29:998-1004.
  7. Palmeira P, Costa-Carvalho BT, Arslanian C et al. Transfer of antibodies across the placenta and breast milk from mothers on intravenous immunoglobulin. Pediatr Allergy Immunol 2009;20:528-35.
  8. Lessa ALS, Krebs VLJ, Brasil TB et al. Preterm and term neonates transplacentally acquire IgG antibodies specific to LPS from Klebsiella pneumonia, Escherichia coli I and Pseudomonas aeruginosa. FEMS Immunol Med Microbiol 2011;62:236-46.
  9. Palmeira P, Quinello C, Silveira-Lessa AL et al. IgG placental transfer in healthy and pathological pregnancies. Clin Develop Immunol 2012;985646:13. DOI: 10.1155/2012/985646. · DOI · Paper page
  10. Kelsch R, Hutt K, Cassens U et al. Semi quantitative measurement of IgG subclasses and IgM of platelet specific antibodies in a glycopro- tein-specific platelet-antigen capture assay. Br J Haematol 2002;117:141-50.
  11. Akilesh S, Huber TB, Wu H et al. Podocytes use FcRn to clear IgG from the glomerular basement membrane. Proc Natl Acad Sci USA 2008;105:967-72.
  12. Montoyo HP, Vaccaro C, Hafner M et al. Conditional deletion of the MHC class I-related receptor FcRn reveals the sites of IgG homeostasis in mice. Proc Natl Acad Sci USA 2009;106:2788-93.
  13. Cui D, Zhang L, Li J et al. Bovine FcRn-mediated human immunoglobulin G transfer across the milk-blood barrier in trans- genic mice. PLoS One 2014;9:e115972.
  14. Leach JL, Sedmak DD, Osborne JM et al. Isolation from human placenta of the IgG transporter, FcRn, and localization to the syncytiotrophoblast: implications for maternal-fetal antibody trans- port. J Immunol 1996;157:3317-22.
  15. American Diabetes Association. (ADA). Diagnosis and classification of diabetes mellitus. Diabetes Care 2012;35:S64-71.
  16. Rudge MVC, Calderon IMP, Ramos MD et al. Perinatal outcome of pregnancies complicated by diabetes and by maternal daily hyper- glycemia not related to diabetes. A retrospective 10-year analysis. Gynaecol Obst Invest 2000;50:108-12.
  17. Calderon IMP, Damasceno DC, Amorin RL et al. Morphometric study of placental villi and vessels in women with mild hyperglycemia or gestational or overt diabetes. Diabetes Res Clin Pract 2007;78:65- 71.
  18. Feng Y, Gong R, Dimitrov DS. Design, expression and character- ization of a soluble single-chain functional human neonatal Fc receptor. Protein Expr Purif 2001;79:66-71.
  19. Lencer WI, Blumberg RS. A passionate kiss, then run: exocytosis and recycling of IgG by FcRn. Trends Cell Biol 2005;15:5-9.
  20. Yeung YA, Leabman MK, Marvin JS et al. Engineering human IgG1 affinity to human neonatal Fc receptor: impact of affinity improve- ment on pharmacokinetics in primates. J Immunol 2009;182:7663-71.
  21. Vidarsson G, Dekkers G, Rispens T. IgG subclasses and allotypes: from structure to effector functions. Frontiers Immunol 2014;5:529.
  22. Menni C, Keser T, Mangino M et al. Glycosylation of immunoglob- ulin g: role of genetic and epigenetic influences. PLoSOne 2013;8: e82558.
  23. Chen G, Wang Y, Qiu L et al. Human IgG Fc-glycosylation profiling reveals associations with age, sex, female sex hormones and thyroid cancer. J Proteomics 2012;75:2824-34.
  24. Costa-Carvalho BT, Vieira HM, Dimantas RB et al. Transfer of IgG subclasses across placenta in term and preterm newborns. Braz J Med Biol Res 1996;29:201-4.
  25. Akbulut H, C ßelik I, C ßelik A et al. Placental transfer of total IgG and IgG subclasses in a Turkish population living in eastern Anatolia. Nobel Med 2012;8:59-64.
  26. Hashira S, Okitsu-Negishi S, Yoshino K. Placental transfer of IgG subclasses in a Japanese population. Pediatr Int 2000;42:337-42.
  27. Sgarbosa F, Barbisan LF, Brasil MA et al. Changes in apoptosis and Bcl-2 expression in human hyperglycemic, term placental tro- phoblast. Diabetes Res Clin Pract 2006;73:143-9.
  28. Rudge MVC, Nero UD, Damasceno DC et al. Influence of weight, volume and mass density of placental weight in newborns of pregnant women with hypertension or diabetes Mellitus. Diabetes Cl ın 2012;5:362-7.
  29. Damasceno DC, Volpato GT, Calderon MPC et al. Oxidative stress and diabetes in pregnant rats. Anim Reprod Sci 2002;72:235-44.
  30. Moreli JB, Morceli G, Magalhães CG et al. Influence of maternal hyperglycemia on IL-10 and TNF-a production: the relationship with perinatal outcomes. J Clin Immuno 2012;32:604-10.
  31. Moreli JB, Santos JH, Rocha CR et al. DNA damage and its cellular response in mother and fetus exposed to hyperglycemic environment. BioMed Res Int 2014; 2014: 676758.
  32. Hara CCP, Franc ßa EL, Fagundes DLG et al. Characterization of natural killer cells and cytokines in maternal placenta and fetus of diabetic mothers. J Immunol Res 2016; 2016: 7154524.
  33. Pietro L, Daher S, Rudge MVC et al. Vascular endothelial growth factor (VEGF) and VEGF-receptor expression in placenta of hyper- glycemic pregnant women. Placenta 2010;31:770-80.
  34. Cvitic S, Desoye G, Hiden U. Glucose, insulin, and oxygen interplay in placental hypervascularisation in diabetes mellitus. BioMed Res Int 2014; 2014: 145846.
  35. Wahabi HA, Esmaeil SA, Fayed A et al. Pre-existing diabetes mellitus and adverse pregnancy outcomes BMC Research. Notes 2012;5:496.

Cited by

Sorted by citations

Authors
de Souza, E. G., Hara, C. C. P., Fagundes, D. L. G., de Queiroz, A. A., Morceli, G., Calderon, I. M. P., França, E. L., & Honorio‐França, A. C.
Affiliations
  • Institute of Biological and Health Science Federal University of Mato Grosso Barra do Garças MT Brazil
  • Universidade Estadual Paulista (Unesp) (BR)
  • Universidade Federal de Mato Grosso (BR)
  • Graduate Program in Gynecology Obstetrics and Mastology Botucatu Medical School São Paulo State University/UNESP Botucatu SP Brazil
ORCID
Venue
Scandinavian Journal of Immunology
Publisher
Wiley
Published
2016-09-26
Volume
84
Issue
4
Pages
237-244
ISSN
  • 0300-9475 (ISSN-L)
  • 1365-3083
DOI
10.1111/sji.12466
OpenAlex
W2501955543
Cited by
22
References
5
Citations by year
2026: 1 · 2025: 3 · 2024: 3 · 2023: 4 · 2022: 3 · 2021: 2 · 2020: 2 · 2019: 1 · 2017: 3
FWCI
1.72
Type
journal article
Language
en
Open access
Licence
onlinelibrary.wiley.com/termsAndConditions#vor (vor)
Locations
  • Scandinavian Journal of Immunology (publishedVersion)
  • PubMed (publishedVersion)
  • UNESP Institutional Repository (São Paulo State University) (submittedVersion)
Funders
  • Fundação de Amparo à Pesquisa do Estado de São Paulo (2012/18033‐0, 2012/24212‐4, 2013/13017‐9)
  • Fundação de Amparo à Pesquisa do Estado de Mato Grosso (158085/2014)
  • Conselho Nacional de Desenvolvimento Científico e Tecnológico (308702/2013‐1, 475238/2013‐3)
Topics
  • Health Sciences › Medicine › Obstetrics and Gynecology › Gestational Diabetes Research and Management
  • Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Monoclonal and Polyclonal Antibodies Research
  • Health Sciences › Medicine › Pediatrics, Perinatology and Child Health › Birth, Development, and Health
Keywords
neonatal Fc receptor, maternal diabetes, type 2 diabetes, umbilical cord blood, placenta, B cells, IgG subclasses, IgG4, transplacental antibody transfer, maternal-fetal antibody transfer, hyperglycemia
MeSH terms
Adult, Cross-Sectional Studies, Diabetes Mellitus, Type 2, Female, Fetal Blood, Fetus, Humans, Immunoglobulin G, Infant, Newborn, Leukocytes, Mononuclear, Maternal-Fetal Exchange, Placenta, Pregnancy, Receptors, Fc, Histocompatibility Antigens Class I, Gene Expression, Diabetes, Gestational, Antigens, CD19

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review