Physical activity ameliorates cartilage degeneration in a rat model of aging: a study on lubricin expression
Open accessAbstract
Osteoarthritis (OA) is a common musculoskeletal disorder characterized by slow progression and joint tissue degeneration. Aging is one of the most prominent risk factors for the development and progression of OA. OA is not, however, an inevitable consequence of aging and age-related changes in the joint can be distinguished from those that are the result of joint injury or inflammatory disease. The question that remains is whether OA can be prevented by undertaking regular physical activity. Would moderate physical activity in the elderly cartilage (and lubricin expression) comparable to a sedentary healthy adult? In this study we used physical exercise in healthy young, adult, and aged rats to evaluate the expression of lubricin as a novel biomarker of chondrocyte senescence. Immunohistochemistry and western blotting were used to evaluate the expression of lubricin in articular cartilage, while enzyme-linked immunosorbent assay was used to quantify lubricin in synovial fluid. Morphological evaluation was done by histology to monitor possible tissue alterations. Our data suggest that moderate physical activity and normal mechanical joint loading in elderly rats improve tribology and lubricative properties of articular cartilage, promoting lubricin synthesis and its elevation in synovial fluid, thus preventing cartilage degradation compared with unexercised adult rats.
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Physical activity intervention in rats ameliorates the deleterious effects of chondrocyte senescence and ageing, indicating that cartilage degeneration responds to environmental signals.
“Physical activity intervention in rats ameliorates the deleterious effects of chondrocyte senescence and ageing [15], indicating that cartilage degeneration responds to environmental signals.”
From Fibrates as drugs with senolytic and autophagic activity for osteoarthritis therapy (2019) · cites this paper as [15]
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Lubricating nano/micro particles for osteoarthritis therapy
Yao, Q., Chen, X., Sheng, H., Zhang, Y., Chen, R., Fan, P., & Kou, L. (2025) · Materials Horizons · doi:10.1039/d5mh01105a · Cited by 26 · see citing works
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Effect of moderate exercise on osteoarthritis
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Review of Soluble Biomarkers of Osteoarthritis: Lessons From Animal Models
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Rapamycin Inhibits Nf-ΚB Activation by Autophagy to Reduce Catabolism in Human Chondrocytes
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Exercise as medicine to be prescribed in osteoarthriti
Ravalli, S., Castrogiovanni, P., & Musumeci, G. (2019) · World Journal of Orthopedics · doi:10.5312/wjo.v10.i7.262 · Cited by 23 · see citing works
Origin and Efficacy of Hyaluronan Injections in Knee Osteoarthritis: Randomized, Double-Blind Trial
Guo, Y., Yang, P., & Liu, L. (2018) · Medical Science Monitor · doi:10.12659/msm.908797 · Cited by 23 · see citing works
“Recombinant lubricin treatment protects articular cartilage and prevents the process of OA [4].”
BackgroundMcCann, M. R., Yeung, C., Pest, M. A., Ratneswaran, A., Pollmann, S. I., Holdsworth, D. W., Beier, F., Dixon, S. J., & Séguin, C. A. (2016) · Osteoarthritis and Cartilage · doi:10.1016/j.joca.2016.11.001 · Cited by 21 · see citing works
Zhao, H., Zhang, H., & Qin, X. (2017) · Molecular Medicine Reports · doi:10.3892/mmr.2017.7838 · Cited by 21 · see citing works
“Physical activity and high‑Tryptophan diet may play important roles in aging‑induced diseases (3,4).”
Banihashemian, S. A., Benisi, S. Z., Hosseinzadeh, S., & Shojaei, S. (2023) · Acta Histochemica · doi:10.1016/j.acthis.2023.152023 · Cited by 18 · see citing works
Chiang, C., Chen, J.-Y., Chiang, M.-Y., Hou, K.-T., Li, W., Chang, S.-J., & Chen, S. (2018) · International Journal of Nanomedicine · doi:10.2147/ijn.s156284 · Cited by 18 · see citing works
“growth factors and mechanical stimulation can promote the maturation of the cells along with the change of biomarkers such as increased lubricin expression in aged rats.(8) As we know, the transforming growth factor-β (TGF-β) family members, especially TGF-β1 and TGF-β3, are widely applied to autologous chondrocytes o…”
Xue, X., Chen, Y., Wang, Y., Zhan, J., Chen, B., Wang, X., & Pan, X. (2019) · Food & Function · doi:10.1039/c9fo01089k · Cited by 17 · see citing works
Petrigna, L., Roggio, F., Trovato, B., Zanghì, M., Guglielmino, C., & Musumeci, G. (2022) · Healthcare · doi:10.3390/healthcare10101821 · Cited by 17 · see citing works
The influence of different sports on cartilage adaptations: A systematic review
Trovato, B., Petrigna, L., Sortino, M., Roggio, F., & Musumeci, G. (2023) · Heliyon · doi:10.1016/j.heliyon.2023.e14136 · Cited by 16 · see citing works
Zhang, D., Zhang, G., Li, Z., & Li, B. (2018) · Experimental Biology and Medicine · doi:10.1177/1535370218757950 · Cited by 16 · see citing works
Post-operative rehabilitation and nutrition in osteoarthritis
Musumeci, G., Mobasheri, A., Trovato, F. M., Szychlinska, M. A., Imbesi, R., & Castrogiovanni, P. (2015) · F1000Research · doi:10.12688/f1000research.4178.2 · Cited by 16 · see citing works
Wang, H., Lin, T.-H., Chang, N.-J., Hsu, H., & Yeh, M. (2019) · International Journal of Molecular Sciences · doi:10.3390/ijms20020259 · Cited by 16 · see citing works
Notopterol alleviates the progression of osteoarthritis: An in vitro and in vivo study
Qu, Y., Qiu, L., Qiu, H., Shen, Y. L., Tang, M., Huang, Y., Peng, Y., Wang, J., & Fu, Q. (2023) · Cytokine · doi:10.1016/j.cyto.2023.156309 · Cited by 15 · see citing works
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“Osteoarthritis (OA) is one of the most common disorders in humans that often leads to persistent pain and inflammation (Arendt-Nielsen et al., 2010; Musumeci et al., 2015; Thudium et al., 2019).”
Musumeci, G. (2016) · Journal of Functional Morphology and Kinesiology · doi:10.3390/jfmk1030269 · Cited by 15 · see citing works
Sun, Z.-P., Wu, S.-P., Liang, C.-D., Zhao, C.-X., & Sun, B. (2019) · Innate Immunity · doi:10.1177/1753425919839125 · Cited by 14 · see citing works
Association of lubricin concentration in synovial fluid and clinical status of osteoarthritic knee
Ogawa, H., Matsumoto, K., Terabayashi, N., Kawashima, K., Takeuchi, K., & Akiyama, H. (2016) · Modern Rheumatology · doi:10.1080/14397595.2016.1209829 · Cited by 13 · see citing works
“In clinical practice, physical therapy, such as rangeof-motion and walking exercises using maximal range of motion, are effective for OA treatment, [13,14] because such exercises may protect articular cartilage by increasing lubricin content in SF, thereby providing boundary lubrication in the diarthroidal joint [9,10…”
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Shahid, M., Manchi, G., Slunsky, P., Naseer, O., Fatima, A. R., Leo, B., & Raila, J. (2017) · Polish Journal of Veterinary Sciences · doi:10.1515/pjvs-2017-0024 · Cited by 13 · see citing works
“The role of lubricin is quite understood in human OA (Musumeci et al. 2014) and in other species, including the rabbit (Elsaid et al. 2005), rat (Musumeci et al. 2015), sheep (Young et al. 2006), equine (Reesink et al. 2016) and guinea pig (Wei et al. 2010).”
BackgroundNorimatsu, K., Nakanishi, K., Ijuin, T., Otsuka, S., Takada, S., Tani, A., Matsuzaki, R., Matsuoka, T., & Sakakima, H. (2023) · Arthritis Research & Therapy · doi:10.1186/s13075-023-03162-z · Cited by 12 · see citing works
“In addition, physical activity promotes lubricin synthesis in the articular cartilage, thereby preventing cartilage degradation in elderly rats [33].”
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RetractedKaiser, J. M., Bernard, F. C., Pucha, K. A., Raval, S. K., Eng, T., Fulton, T. M., Anderson, S. E., Allen, K. D., Dixon, J. B., & Willett, N. J. (2024) · Osteoarthritis and Cartilage · doi:10.1016/j.joca.2024.03.120 · Cited by 8 · see citing works
Early Signs of Bone and Cartilage Changes Induced by Treadmill Exercise in Rats
Moshtagh, P. R., Korthagen, N. M., Plomp, S. G. M., Pouran, B., Castelein, R. M., ZADPOOR, A. A., & Weinans, H. (2017) · JBMR Plus · doi:10.1002/jbm4.10029 · Cited by 8 · see citing works
“This could also elucidate both how the involved tissues improved after exercise,((6,7)) and how they start to become damaged in cases where the exercise reaches a state of overloading.”
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Proteoglycan 4 (PRG4)/Lubricin and the Extracellular Matrix in Gout
Elsaid, K. A., Jay, G. D., Liu‐Bryan, R., & Terkeltaub, R. A. (2023) · Gout Urate and Crystal Deposition Disease · doi:10.3390/gucdd1030012 · Cited by 6 · see citing works
“These collective observations support PRG4’s immunomodulatory and anti-inflammatory roles in the joint.”
Lauretta, G., Ravalli, S., Szychlinska, M. A., Castorina, A., Maugeri, G., D’Amico, A. G., D’Agata, V., & Musumeci, G. (2020) · PubMed · doi:10.14670/hh-18-233 · Cited by 7 · see citing works
Petrigna, L., Trovato, B., Roggio, F., Castorina, A., & Musumeci, G. (2023) · International Journal of Molecular Sciences · doi:10.3390/ijms24043662 · Cited by 6 · see citing works
Welcome to the New Open Access Journal of Functional Morphology and Kinesiology
Musumeci, G. (2015) · Journal of Functional Morphology and Kinesiology · doi:10.3390/jfmk1010001 · Cited by 6 · see citing works
Clinical effect and mechanism of aerobic exercise for knee osteoarthritis: a mini review
Hu, H., Jia, L., Zhao, W.-Y., Guo, S.-J., Fang, Z.-M., Li, X., Zheng, Y.-L., & Chen, P. (2025) · Frontiers in Physiology · doi:10.3389/fphys.2025.1708750 · Cited by 5 · see citing works
Rosa, M. D., Castrogiovanni, P., Trovato, F. M., Malatino, L., Ravalli, S., Imbesi, R., Szychlinska, M. A., & Musumeci, G. (2019) · Applied Sciences · doi:10.3390/app9061041 · Cited by 5 · see citing works
Yin, Y., Zhang, Y., Guo, L., Li, P., Wang, D., Huang, L., Zhao, X.-Q., Wu, G., Li, L., & Wei, X. (2023) · Diagnostics · doi:10.3390/diagnostics13203193 · Cited by 5 · see citing works
“Moderate physical activity and normal mechanical joint load in older rats can improve the tribology and lubrication properties of the articular cartilage, thereby preventing cartilage degradation [37].”
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Protective Effect of Indian Herbs and Physical Exercise on Osteoporosis: A Review
Kaur, P., Bains, K., & Kaur, H. (2021) · Food Reviews International · doi:10.1080/87559129.2021.1926483 · Cited by 4 · see citing works
Cui, J., Shibata, Y., Itaka, K., Zhou, J. M., & Zhang, J. (2022) · Frontiers in Physiology · doi:10.3389/fphys.2022.974266 · Cited by 3 · see citing works
“…for knee OA, including anaerobic, aerobic, flexibility workouts, and aquatic exercise, inducing the benefits on knee-related and health-related outcomes, such as pain release and joint function and life quality improvements (Hunter and Eckstein, 2009; Musumeci et al., 2015; Collins et al., 2019).”
Background
- Authors
- Musumeci, G., Castrogiovanni, P., Trovato, F. M., Imbesi, R., Giunta, S., Szychlinska, M. A., Loreto, C., Castorina, S., & Mobasheri, A.
- Affiliations
- University of Catania (IT)
- University of Nottingham (GB)
- Corresponding author
- Musumeci, G.
- ORCID
- Venue
- Scandinavian Journal of Medicine & Science in Sports
- Publisher
- Wiley
- Published
- 2014-07-09
- Volume
- 25
- Issue
- 2
- Pages
- e222-30
- ISSN
- 0905-7188 (ISSN-L)
- 1600-0838
- OpenAlex
- W2125680519
- Cited by
- 115
- References
- 35
- Citations by year
- 2025: 3 · 2024: 2 · 2023: 14 · 2022: 5 · 2021: 6 · 2020: 6 · 2019: 28 · 2018: 12 · 2017: 15 · 2016: 12 · 2015: 10 · 2014: 2
- FWCI
- 10.79
- Type
- journal article
- Language
- en
- Open access
- green
- hdl.handle.net
- Licence
- Locations
- Scandinavian Journal of Medicine and Science in Sports (publishedVersion)
- PubMed (publishedVersion)
- Nova Science Publishers (Nova Science Publishers, Inc.) (submittedVersion, other-oa)
- Open Research (University of Surrey) (submittedVersion)
- Surrey Research Insight Open Access (The University of Surrey) (submittedVersion)
- Topics
- Health Sciences › Medicine › Rheumatology › Osteoarthritis Treatment and Mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Proteoglycans and glycosaminoglycans research
- Health Sciences › Medicine › Surgery › Hip disorders and treatments
- Keywords
- osteoarthritis, physical activity, aging, lubricin, articular cartilage degeneration, chondrocyte senescence, synovial fluid, cartilage lubrication, biomarkers, rat model
- MeSH terms
- Proteoglycan 4, Aging, Animals, Cartilage, Articular, Glycoproteins, Immunohistochemistry, Male, Motor Activity, Synovial Fluid, Blotting, Western, Biomarkers, Rats, Wistar, Chondrocytes, Rats
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