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Cited by 11Wound Healing and TreatmentsAdvancements in Transdermal Drug DeliveryDermatology and Skin Diseases

The Effect of Polynucleotide-Hyaluronic Acid Hydrogel in the Recovery After Mechanical Skin Barrier Disruption

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Ha, Ye Jin, Tak, Ka Hee, Jung, Jin‐Min, Lee, Jong Lyul, Kim, Chan Wook, Ah, Young‐Chang, Kim, Seok‐Soon, Moon, Ik Jun +1 more·2024·Skin Research and Technology
DOI 10.1111/srt.70068PMC PMC11413333
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Abstract

Background: The epidermal barrier acts as a defense against external agents as well as helps to maintain body homeostasis. Polynucleotides (PN), exogenous DNA fragments, promote wound repair through their stimulatory and anti‐inflammatory effects. Recent findings indicate a synergistic effect of PN and hyaluronic acid (HA) combinations in regulating inflammation and promoting cell proliferation. This study aims to elucidate the effects of PN and HA on repairing the epidermal barrier following its disruption by tape stripping (TS) in a mouse model. Materials and Methods: After disrupting the epidermal barrier using TS, a formulation containing PN (14 mg/mL) and HA (6 mg/mL) was applied. Trans‐epidermal water loss (TEWL) was measured at 0, 3, 6, 24, 48, and 72 h. Mice were euthanized after the final application at 72 h, and tissue samples were analyzed for epidermal/dermal thickness, neutrophil infiltration, and filaggrin expression. Results: We observed a significant reduction in TEWL in the PN+HA group compared to that in the control group (20.8 ± 0.5 vs. 43.7 ± 0.5 g/m 2 h at 72 h, p < 0.05), indicating an improvement in barrier function. Histological evaluation showed decreased epidermal and dermal thickening in the PN+HA group compared to that in the control group (epidermal: 29.4 ± 2.2 vs. 57.9 ± 3.5 μm; dermal: 464.8 ± 25.9 vs. 825.9 ± 44.8 μm, both p < 0.05). Additionally, neutrophil infiltration in the dermis was significantly reduced, and filaggrin protein levels were significantly higher in the PN+HA group compared to those in the control group (4.8 ± 0.4 vs. 21.1 ± 3.3 for neutrophils; 0.84 ± 0.04 vs. 0.42 ± 0.03 for filaggrin, both p < 0.05). Conclusion: These results suggest that PN+HA may be an effective therapeutic strategy for repairing skin barrier damage.

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Research works printed but not linked: 31

  1. [1]R. C. Gupta, R. Lall, A. Srivastava, and A. Sinha, “ Hyaluronic Acid: Molecular Mechanisms and Therapeutic Trajectory,” Frontiers in Veterinary Science 6 (2019): 192. 31294035 PMC6603175
  2. [2]M. N. Leite and M. A. C. Frade, “ Efficacy of 0.2% Hyaluronic Acid in the Healing of Skin Abrasions in Rats,” Heliyon 7, no. 7 (2021): e07572. 34345742 PMC8319016
  3. [3]A. Pilloni, M. A. Rojas, C. Trezza, et al., “ Clinical Effects of the Adjunctive use of Polynucleotide and Hyaluronic Acid‐Based Gel in the Subgingival Re‐Instrumentation of Residual Periodontal Pockets: A Randomized, Split‐Mouth Clinical Trial,” Journal of Periodontology 94, no. 3 (2023): 354– 363. 36189651
  4. [4]M. Cavallini, E. Bartoletti, L. Maioli, et al., “ Consensus Report on the Use of PN‐HPT (Polynucleotides Highly Purified Technology) in Aesthetic Medicine,” Journal of Cosmetic Dermatology 20, no. 3 (2021): 922– 928. 32799391 PMC7984045
  5. [5]K. S. Lee, S. Lee, H. Wang, et al., “ Analysis of Skin Regeneration and Barrier‐Improvement Efficacy of Polydeoxyribonucleotide Isolated From Panax Ginseng (C.A. Mey.) Adventitious Root,” Molecules (Basel, Switzerland) 28, no. 21 (2023): 7240. 37959659 PMC10649580
  6. [6]S. Segarra, I. Bošnjak, I. Mioč, et al., “ Yeast‐Derived Nucleotides Enhance Fibroblast Migration and Proliferation and Provide Clinical Benefits in Atopic Dermatitis,” International Journal of Molecular Sciences 25, no. 5 (2024): 2890. 38474135 PMC10932167
  7. [7]G. Picciolo, F. Mannino, N. Irrera, et al., “ PDRN, a Natural Bioactive Compound, Blunts Inflammation and Positively Reprograms Healing Genes in an “In Vitro” Model of Oral Mucositis,” Biomedicine & Pharmacotherapy 138 (2021): 111538. 34311536
  8. [8]S. Jo, A. Baek, Y. Cho, et al., “ Therapeutic Effects of Polydeoxyribonucleotide in an In Vitro Neuronal Model of Ischemia/Reperfusion Injury,” Scientific Reports 13, no. 1 (2023): 6004. 37045900 PMC10097812
  9. [9]D. Dallari, G. Sabbioni, N. Del Piccolo, et al., “ Efficacy of Intra‐Articular Polynucleotides Associated With Hyaluronic Acid Versus Hyaluronic Acid Alone in the Treatment of Knee Osteoarthritis: A Randomized, Double‐Blind, Controlled Clinical Trial,” Clinical Journal of Sport Medicine 30, no. 1 (2020): 1– 7. 31855906
  10. [10]G. De Caridi, M. Massara, I. Acri, et al., “ Trophic Effects of Polynucleotides and Hyaluronic Acid in the Healing of Venous Ulcers of the Lower Limbs: A Clinical Study,” International Wound Journal 13, no. 5 (2016): 754– 758. 25224018 PMC7949893
  11. [12]M. T. Colangelo, S. Belletti, P. Govoni, S. Guizzardi, and C. Galli, “ A Biomimetic Polynucleotides‐Hyaluronic Acid Hydrogel Promotes Wound Healing in a Primary Gingival Fibroblast Model,” Applied Sciences (Basel) 11, no. 10 (2021): 4405.
  12. [13]A. J. Hughes, S. S. Tawfik, K. P. Baruah, E. A. O'Toole, and R. F. L. O'Shaughnessy, “ Tape Strips in Dermatology Research,” British Journal of Dermatology 185, no. 1 (2021): 26– 35. 33370449
  13. [14]M. Denda, L. C. Wood, S. Emami, et al., “ The Epidermal Hyperplasia Associated With Repeated Barrier Disruption by Acetone Treatment or Tape Stripping Cannot be Attributed to Increased Water Loss,” Archives of Dermatological Research 288, no. 5‐6 (1996): 230– 238. 8738565
  14. [15]M. Demerjian, M. Q. Man, E. H. Choi, et al., “ Topical Treatment With Thiazolidinediones, Activators of Peroxisome Proliferator‐Activated Receptor‐Gamma, Normalizes Epidermal Homeostasis in a Murine Hyperproliferative Disease Model,” Experimental Dermatology 15, no. 3 (2006): 154– 160. 16480422
  15. [16]S. K. Stacey and M. McEleney, “ Topical Corticosteroids: Choice and Application,” American Family Physician 103, no. 6 (2021): 337– 343. 33719380
  16. [17]D. E. Cohen and N. Heidary, “ Treatment of Irritant and Allergic Contact Dermatitis,” Dermatologic Therapy 17, no. 4 (2004): 334– 340. 15327479
  17. [18]J. I. Silverberg, A. Armstrong, A. Blauvelt, and K. Reich, “ Assessment of Efficacy and Safety Outcomes Beyond Week 16 in Clinical Trials of Systemic Agents Used for the Treatment of Moderate to Severe Atopic Dermatitis in Combination With Topical Corticosteroids,” American Journal of Clinical Dermatology 24, no. 6 (2023): 913– 925. 37695504 PMC10570226
  18. [19]A. B. Mehta, N. J. Nadkarni, S. P. Patil, K. V. Godse, M. Gautam, and S. Agarwal, “ Topical Corticosteroids in Dermatology,” Indian Journal of Dermatology, Venereology and Leprology 82, no. 4 (2016): 371– 378. 27279294
  19. [20]U. Darsow, A. Wollenberg, D. Simon, et al., “ ETFAD/EADV Eczema Task Force 2009 Position Paper on Diagnosis and Treatment of Atopic Dermatitis,” Journal of the European Academy of Dermatology and Venereology 24, no. 3 (2010): 317– 328. 19732254
  20. [21]V. Voinchet, P. Vasseur, and J. Kern, “ Efficacy and Safety of Hyaluronic Acid in the Management of Acute Wounds,” American Journal of Clinical Dermatology 7, no. 6 (2006): 353– 357. 17173469
  21. [22]H. Roehrs, J. G. Stocco, F. Pott, G. Blanc, M. J. Meier, and F. A. Dias, “ Dressings and Topical Agents Containing Hyaluronic Acid for Chronic Wound Healing,” Cochrane Database of Systematic Reviews (Online) 7, no. 7 (2023): Cd012215. PMC10373121 37497805
  22. [23]H.‐Y. Park, D.‐K. Kweon, and J.‐K. Kim, “ Upregulation of Tight Junction‐Related Proteins by Hyaluronic Acid in human HaCaT Keratinocytes,” Bioactive Carbohydrates and Dietary Fibre 30 (2023): 100374.
  23. [24]F. Della Sala, A. Fabozzi, M. di Gennaro, et al., “ Advances in Hyaluronic‐Acid‐Based (Nano) Devices for Cancer Therapy,” Macromolecular Bioscience 22, no. 1 (2022): e2100304. 34657388
  24. [25]S. Vasvani, P. Kulkarni, and D. Rawtani, “ Hyaluronic Acid: A Review on its Biology, Aspects of Drug Delivery, Route of Administrations and a Special Emphasis on its Approved Marketed Products and Recent Clinical Studies,” International Journal of Biological Macromolecules 151 (2020): 1012– 1029. 31715233
  25. [27]M. Yu and J. Y. Lee, “ Polydeoxyribonucleotide Improves Wound Healing of Fractional Laser Resurfacing in Rat Model,” Journal of Cosmetic and Laser Therapy 19, no. 1 (2017): 43– 48. 27762652
  26. [28]A. M. O. Pratama and A. R. Muharram, “ Salmon DNA Combination for Hypertrohic Scar Treatment With Diabetes Mellitus Type 2: A Case Report,” International Journal of Medical Science and Clinical Research Studies 2, no. 8 (2022): 846– 849.
  27. [29]A. Araco, F. Araco, and M. Raichi, “ Clinical Efficacy and Safety of Polynucleotides Highly Purified Technology (PN‐HPT®) and Cross‐Linked Hyaluronic Acid for Moderate to Severe Nasolabial Folds: A Prospective, Randomized, Exploratory Study,” Journal of Cosmetic Dermatology 22, no. 1 (2023): 146– 155. 35531796 PMC10084116
  28. [30]S. Jang, J. Ohn, J. W. Kim, et al., “ Caffeoyl‐Pro‐His Amide Relieve DNCB‐Induced Atopic Dermatitis‐Like Phenotypes in BALB/c Mice,” Scientific Reports 10, no. 1 (2020): 8417. 32439906 PMC7242424
  29. [31]T. Ear and P. P. McDonald, “ Cytokine Generation, Promoter Activation, and Oxidant‐Independent NF‐kappaB Activation in a Transfectable Human Neutrophilic Cellular Model,” BMC Immunology [Electronic Resource] 9 (2008): 14. 18405381 PMC2322942
  30. [33]W. Peng and N. Novak, “ Pathogenesis of Atopic Dermatitis,” Clinical and Experimental Allergy 45, no. 3 (2015): 566– 574. 25610977
  31. [34]Y. Chen, X. Li, X. Gan, et al., “ Fucoidan From Undaria Pinnatifida Ameliorates Epidermal Barrier Disruption via Keratinocyte Differentiation and CaSR Level Regulation,” Marine Drugs 17, no. 12 (2019): 660. 31771286 PMC6950751

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Authors
Ha, Y. J., Tak, K. H., Jung, J., Lee, J. L., Kim, C. W., Ah, Y., Kim, S., Moon, I. J., & Yoon, Y. S.
Affiliations
  • Asan Medical Center (KR)
  • BRPHARM Co., Ltd Seoul South Korea
Corresponding author
Yoon, Yong Sik
ORCID
Venue
Skin Research and Technology
Publisher
Wiley
Published
2024-09
Volume
30
Issue
9
Pages
e70068-e70068
ISSN
  • 0909-752X (ISSN-L)
  • 1600-0846
DOI
10.1111/srt.70068
PMC
PMC11413333
OpenAlex
W4402692293
Cited by
11
References
3
Citations by year
2026: 7 · 2025: 4
FWCI
1.73
Type
journal article
Language
en
Open access
Licence
Locations
  • Skin Research and Technology (publishedVersion, cc-by-nc-nd)
  • PubMed Central (publishedVersion, cc-by-nc-nd)
  • PubMed (publishedVersion)
Funders
Asan Institute for Life Sciences, Asan Medical Center (2020IF0009, 2024IP0034)
Topics
  • Health Sciences › Medicine › Rehabilitation › Wound Healing and Treatments
  • Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Pharmaceutical Science › Advancements in Transdermal Drug Delivery
  • Health Sciences › Medicine › Dermatology › Dermatology and Skin Diseases
Keywords
polynucleotides, hyaluronic acid, skin barrier repair, skin barrier disruption, tape stripping, transepidermal water loss, filaggrin, neutrophil infiltration, epidermal thickness, skin thickness, anti-inflammatory activity, wound healing
MeSH terms
Filaggrin Proteins, Animals, Disease Models, Animal, Epidermis, Hyaluronic Acid, Intermediate Filament Proteins, Male, Polynucleotides, Skin, Water Loss, Insensible, Wound Healing, Hydrogels, Mice

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