Effect of oestrogen on myofibre size and myosin expression in growing rats
Abstract
This study examined the effect of oestrogen deprivation and replacement on plantaris muscle size and myosin heavy chain (MHC) isoform composition in rats during a period of physiological growth. Seven‐week‐old female Sprague–Dawley rats were assigned to one of the three treatment groups: (1) control animals (Sham); (2) ovariectomized animals without oestrogen replacement (OVX/CO); and (3) ovariectomized animals with 17β‐oestradiol replacement (OVX/E 2). OVX/CO and OVX/E 2 animals were pair‐fed with Sham animals to rule out the potentially confounding effects of differences in food intake and weight gain. Rats were killed 4 weeks after surgery and the plantaris muscle was removed for analysis. Ovariectomy had no effect on muscle fibre size, but reduced the relative amount of type IIx MHC. This was reversed with oestrogen replacement, suggesting that the reduction in type IIx MHC expression was an oestrogen‐mediated effect. Oestrogen replacement reduced type IIb MHC expression and fast muscle fibre size. Changes in fast fibre size and type IIb MHC expression were not seen with ovariectomy, indicating that these changes were not simply due to the presence of oestrogen in the ovariectomized, oestrogen‐replaced animals. These results suggest that another ovarian hormone may counteract the effect of oestrogen on fast fibre size and type IIb MHC expression in intact animals.
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Hormonal regulation of skeletal muscle development and contractility are well documented.
“In fact, hormonal regulation of skeletal muscle development and contractility are well documented (29, 70).”
From Sex-Based Differences in Skeletal Muscle Kinetics and Fiber-Type Composition (2015) · cites this paper as [70]
In the mouse plantaris muscle, OVX leads to a reduction in the relative amount of type-IIX fibers from 38% in sham animals to 33%.
“However, in the mouse plantaris muscle, OVX does lead to a reduction in the relative amount of type-IIX fibers from 38% in sham animals to 33% (70).”
From Sex-Based Differences in Skeletal Muscle Kinetics and Fiber-Type Composition (2015) · cites this paper as [70]
Supplementation with estrogen increases the type-IIX percentage composition in the plantaris back to 42%.
“Supplementation with estrogen increases the type-IIX percentage composition in the plantaris back to 42% (70).”
From Sex-Based Differences in Skeletal Muscle Kinetics and Fiber-Type Composition (2015) · cites this paper as [70]
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Sex-Based Differences in Skeletal Muscle Kinetics and Fiber-Type Composition
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- Authors
- Piccone, C. M., Brazeau, G. A., & McCormick, K. M.
- Affiliations
- University at Buffalo, State University of New York (US)
- ORCID
- Brazeau, Gayle A.
- Venue
- Experimental Physiology
- Publisher
- Wiley
- Published
- 2004-12-21
- Volume
- 90
- Issue
- 1
- Pages
- 87-93
- ISSN
- 0958-0670 (ISSN-L)
- 1469-445X
- OpenAlex
- W2115568123
- Cited by
- 46
- References
- 33
- Citations by year
- 2026: 1 · 2025: 1 · 2024: 1 · 2023: 1 · 2022: 2 · 2020: 2 · 2019: 1 · 2018: 5 · 2017: 4 · 2016: 3 · 2015: 1 · 2014: 2 · 2013: 3 · 2012: 2
- FWCI
- 0.59
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Experimental Physiology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Estrogen and related hormone effects
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Hormonal and reproductive studies
- Keywords
- estrogen, ovariectomy, estrogen replacement therapy, estradiol, myosin heavy chain isoforms, plantaris muscle, muscle growth, ovarian hormones, female rats
- MeSH terms
- Aging, Animals, Estradiol, Myosins, Organ Size, Ovariectomy, Rats, Sprague-Dawley, Muscle, Skeletal, Muscle Fibers, Skeletal, Gene Expression Regulation, Developmental, Myosin Heavy Chains, Hormone Replacement Therapy, Rats
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