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Cited by 812Ion channel regulation and functionNicotinic Acetylcholine Receptors StudyNeuroscience and Neuropharmacology Research

A patch-clamp study of bovine chromaffin cells and of their sensitivity to acetylcholine

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Fenwick, E M, Marty, A, Neher, E·1982·The Journal of Physiology
DOI 10.1113/jphysiol.1982.sp014393PMC PMC1197770
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Abstract

1. Bovine chromaffin cells were enzymatically isolated and kept in short term tissue culture. Their electrical properties were studied using recent advances of the patch-clamp technique (Hamill, Marty, Neher, Sakmann & Sigworth, 1981). 2. When a patch pipette was sealed tightly to a chromaffin cell ('cell-attached configuration') current wave forms due to intracellular action potentials could be observed. The frequency of the wave forms was altered by changing the pipette potential. When acetylcholine was present in the pipette solution, acetylcholine-induced single channel currents were evident in the patch recording. Action potential wave forms were then often seen to follow acetycholine-induced single channel currents. 3. In the cell-attached configuration, large single channel current events did not resemble square pulses but showed exponential relaxations with time constants of the order of 50 ms. 4. After rupture of the patch of membrane, the pipette--cell seal remained stable ('whole-cell recording', Hamill et al. 1981). Chromaffin cells were found to have a resting potential of -50 to -80 mV, and an input resistance around 5 G omega. The high cell resistance accounts for the relaxing currents evident in the cell-attached configuration. 5. In the best cases, the effective time constant of the voltage clamp in the whole-cell recording mode was 15 microseconds. Exchange of small ions such as Na+ ions between pipette and cell interior solutions was then complete within 15 s. 6. Acetylcholine-induced currents were obtained at various acetylcholine concentrations. Single acetylcholine-induced channels had a slope conductance of 44 pS between -100 and -55 mV, and a mean duration of 27 ms at -80 mV (at room temperature).

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  • Adrenal chromaffin cells are classic neuroendocrine cells that secrete catecholamines upon receiving stimulation from sympathetic nerves.

    “Adrenal chromaffin cells are classic neuroendocrine cells, which secrete catecholamines upon receiving stimulation from sympathetic nerves (Fenwick et al., 1982; Sala et al., 2008).”

    From Intestinal microbiota modulates adrenomedullary response through Nod1 sensing in chromaffin cells (2021) · cites this paper as Fenwick et al., 1982

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Authors
Fenwick, E. M., Marty, A., & Neher, E.
ORCID
Venue
The Journal of Physiology
Publisher
Wiley
Published
1982-10
Volume
331
Issue
1
Pages
577-597
ISSN
  • 0022-3751 (ISSN-L)
  • 1469-7793
DOI
10.1113/jphysiol.1982.sp014393
PMC
PMC1197770
OpenAlex
W2137760362
Cited by
812
References
23
Citations by year
2026: 5 · 2025: 1 · 2024: 5 · 2023: 4 · 2022: 8 · 2021: 6 · 2020: 7 · 2019: 6 · 2018: 7 · 2017: 8 · 2016: 5 · 2015: 8 · 2014: 10 · 2013: 6 · 2012: 8
FWCI
11.56
Type
journal article
Language
en
Open access
Licence
onlinelibrary.wiley.com/termsAndConditions#vor (vor)
Locations
  • The Journal of Physiology (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ion channel regulation and function
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nicotinic Acetylcholine Receptors Study
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
Keywords
chromaffin cells, acetylcholine, acetylcholine-induced currents, single-channel currents, action potential, resting membrane potential, input resistance, voltage clamp, ion exchange, bovine adrenal medulla
MeSH terms
Acetylcholine, Action Potentials, Adrenal Medulla, Animals, Cattle, Cells, Cultured, Chromaffin System, Electric Conductivity, Ion Channels, Membrane Potentials, Microelectrodes

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