Oxidation induces a Cl−‐dependent cation conductance in human red blood cells
Open accessAbstract
Oxidative stress induces complex alterations of membrane proteins in red blood cells (RBCs) eventually leading to haemolysis. To study changes of membrane ion permeability induced by oxidative stress, whole‐cell patch‐clamp recordings and haemolysis experiments were performed in control and oxidised human RBCs. Control RBCs exhibited a small cation‐selective whole‐cell conductance (236 ± 38 pS; n= 8) which was highly sensitive to the external Cl− concentration: replacement of NaCl in the bath by sodium gluconate induced an increase of this cation conductance by about 85 %. Exposing RBCs to t‐butylhydroxyperoxide (1 m m for 10 min) induced a twofold increase in this cation conductance which was further stimulated after replacement of extracellular Cl− by gluconate, Br−, I− or SCN−. In addition, lowering the ionic strength of the bath solution by isosmotic substitution of NaCl by sorbitol activated the cation conductance. The Cl−‐sensitive and oxidation‐induced cation conductance was Ca 2+ permeable, exhibited a permselectivity of Cs+ > K+ > Na+= Li+ >> NMDG+, and was partially inhibited by amiloride (1 m m) and almost completely inhibited by GdCl 3 (150 μ m), but was insensitive to TEA, BaCl 2, NPPB, flufenamic acid or quinidine. DIDS (100 μ m) reversibly inhibited the activation of the cation conductance by removal of external Cl−. Oxidation induced haemolysis in NaCl‐bathed human RBCs. This haemolysis was attenuated by amiloride (1 m m) and inhibited by replacement of bath Na+ by the impermeant cation NMDG+. The Na+‐ and Ca 2+‐permeable conductance might be involved in haemolytic diseases induced by elevated oxidative stress, such as glucose‐6‐phosphate dehydrogenase deficiency.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Malfunction of antioxidant defense and/or conditions of increased oxidant production have severe consequences for RBCs on a subcellular level.
“Malfunction of antioxidant defense and/or conditions of increased oxidant production have severe consequences for RBCs on a subcellular level.”
From Red Blood Cell Function and Dysfunction: Redox Regulation, Nitric Oxide Metabolism, Anemia (2017) · cites this paper as [49]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
Huber, S. M., Gamper, N. L., & Lang, F. J. W. (2001) · Pflügers Archiv - European Journal of Physiology · doi:10.1007/s004240000456 · Cited by 175 · see citing works
Self-citationGrygorczyk, R., & Schwarz, W. H. (1983) · Cell Calcium · doi:10.1016/0143-4160(83)90025-8 · Cited by 111 · see citing works
Kaestner, L., Christophersen, P., Bernhardt, I., et al. (2000) · Bioelectrochemistry · doi:10.1016/s0302-4598(00)00110-0 · Cited by 111 · see citing works
The Passive Permeability of the Red Blood Cell to Cations
LaCelle, P. L., & Rothstein, A. (1966) · The Journal of General Physiology · doi:10.1085/jgp.50.1.171 · Cited by 105 · see citing works
Lipid peroxidation of human erythrocyte ghosts induced by organic hydroperoxides
Koster, J. F., & Slee, R. G. (1983) · Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism · doi:10.1016/0005-2760(83)90117-0 · Cited by 101 · see citing works
Ingrosso, D., D’Angelo, S., Carlo, E. D., et al. (2000) · European Journal of Biochemistry · doi:10.1046/j.1432-1327.2000.01485.x · Cited by 86 · see citing works
Evidence for a voltage-gated, non-selective cation channel in the human red cell membrane
Christophersen, P., & Bennekou, P. (1991) · Biochimica et Biophysica Acta (BBA) - Biomembranes · doi:10.1016/0005-2736(91)90017-3 · Cited by 86 · see citing works
Jones, G. S., & Knauf, P. A. (1985) · The Journal of General Physiology · doi:10.1085/jgp.86.5.721 · Cited by 83 · see citing works
Non-selective voltage-activated cation channel in the human red blood cell membrane
Kaestner, L., Bollensdorff, C., & Bernhardt, I. (1999) · Biochimica et Biophysica Acta (BBA) - Biomembranes · doi:10.1016/s0005-2736(98)00240-5 · Cited by 73 · see citing works
Effects of low ionic strength media on passive human red cell monovalent cation transport.
Bernhardt, I., Hall, A. C., & Ellory, J. C. (1991) · The Journal of Physiology · doi:10.1113/jphysiol.1991.sp018482 · Cited by 70 · see citing works
Mavelli, I., Ciriolo, M. R., Rossi, L., et al. (1984) · European Journal of Biochemistry · doi:10.1111/j.1432-1033.1984.tb07969.x · Cited by 69 · see citing works
Novel Anion Dependence of Induced Cation Transport in Malaria-infected Erythrocytes
Kirk, K., & Horner, H. A. (1995) · Journal of Biological Chemistry · doi:10.1074/jbc.270.41.24270 · Cited by 66 · see citing works
The voltage-gated non-selective cation channel from human red cells is sensitive to acetylcholine
Bennekou, P. (1993) · Biochimica et Biophysica Acta (BBA) - Biomembranes · doi:10.1016/0005-2736(93)90328-w · Cited by 63 · see citing works
Christophersen, P. (1991) · The Journal of Membrane Biology · doi:10.1007/bf01868542 · Cited by 62 · see citing works
Dinudom, A., Komwatana, P., Young, J. A., et al. (1995) · The Journal of Physiology · doi:10.1113/jphysiol.1995.sp020899 · Cited by 57 · see citing works
Antioxidant capacity of G-6-PD-deficient erythrocytes
Bilmen, S., Aksu, T. A., Gümüşlü, S., et al. (2001) · Clinica Chimica Acta · doi:10.1016/s0009-8981(00)00384-3 · Cited by 53 · see citing works
A Novel Pathway for Ca++ Entry into Plasmodium falciparum-Infected Blood Cells
Desai, S. A., McCleskey, E. W., Schlesinger, P. H., et al. (1996) · American Journal of Tropical Medicine and Hygiene · doi:10.4269/ajtmh.1996.54.464 · Cited by 48 · see citing works
Wagner, C. A., Bröer, A., Albers, A., Gamper, N. L., Lang, F., & Bröer, S. (2000) · The Journal of Physiology · doi:10.1111/j.1469-7793.2000.00035.x · Cited by 45 · see citing works
Self-citationTurrini, F., Naitana, A. F., Mannuzzu, L., et al. (1985) · Blood · doi:10.1182/blood.v66.2.302.302 · Cited by 44 · see citing works
The monovalent cation "leak" transport in human erythrocytes: an electroneutral exchange process
Richter, S., Hamann, J., Kummerow, D., et al. (1997) · Biophysical Journal · doi:10.1016/s0006-3495(97)78106-2 · Cited by 43 · see citing works
Kummerow, D., Hamann, J., Browning, J., et al. (2000) · The Journal of Membrane Biology · doi:10.1007/s00232001089 · Cited by 38 · see citing works
Activation of a novel organic solute transporter in mammalian red blood cells.
Culliford, S. J., Bernhardt, I., & Ellory, J. C. (1995) · The Journal of Physiology · doi:10.1113/jphysiol.1995.sp021089 · Cited by 23 · see citing works
Kummerow, D., Hamann, J., Browning, J. A., et al. (2000) · The Journal of Membrane Biology · doi:10.1007/s002320001089 · Cited by 17 · see citing works
Motor unit discharge behaviors in stroke patients
Yan, K., Fang, J., & Shahani, B. T. (1998) · Muscle & Nerve · doi:10.1002/(sici)1097-4598(199811)21:11 · Cited by 12 · see citing works
Bennekou, P., & Christophersen, P. (1990) · Biochimica et Biophysica Acta (BBA) - Biomembranes · doi:10.1016/0005-2736(90)90255-m · Cited by 12 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
(Patho)physiological Significance of the Serum- and Glucocorticoid-Inducible Kinase Isoforms
Lang, F., Böhmer, C., Palmada, M., Seebohm, G., Strutz-Seebohm, N., & Vallon, V. (2006) · Physiological Reviews · doi:10.1152/physrev.00050.2005 · Cited by 689 · see citing works
Self-citationRed Blood Cell Function and Dysfunction: Redox Regulation, Nitric Oxide Metabolism, Anemia
Kuhn, V., Diederich, L., Keller, T. C. S., Kramer, C. M., Lückstädt, W., Panknin, C., Suvorava, T., Isakson, B. E., Kelm, M., & Cortese-Krott, M. M. (2017) · Antioxidants & Redox Signaling · doi:10.1089/ars.2016.6954 · Cited by 469 · see citing works
Oxidative stress, eryptosis and anemia: a pivotal mechanistic nexus in systemic diseases
Bissinger, R., Bhuyan, A. A. M., Qadri, S. M., & Lang, F. (2019) · The FEBS Journal · doi:10.1111/febs.14606 · Cited by 239 · see citing works
Self-citation
- Authors
- Duranton, C., Huber, S. M., & Lang, F.
- Affiliations
- University of Tübingen (DE)
- Corresponding author
- Duranton, Christophe
- Venue
- The Journal of Physiology
- Publisher
- Wiley
- Published
- 2002-03
- Volume
- 539
- Issue
- 3
- Pages
- 847-855
- ISSN
- 0022-3751 (ISSN-L)
- 1469-7793
- PMC
- PMC2290198
- OpenAlex
- W2039659095
- Cited by
- 233
- References
- 25
- Citations by year
- 2026: 1 · 2025: 2 · 2024: 3 · 2023: 8 · 2022: 4 · 2021: 5 · 2020: 3 · 2019: 6 · 2018: 3 · 2017: 2 · 2016: 4 · 2015: 4 · 2014: 7 · 2013: 10 · 2012: 10
- FWCI
- 9.19
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- The Journal of Physiology (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Physiology › Erythrocyte Function and Pathophysiology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ion Transport and Channel Regulation
- Keywords
- oxidative stress, human erythrocytes, cation conductance, hemolysis, membrane ion permeability, calcium permeability, amiloride, gadolinium, DIDS, glucose-6-phosphate dehydrogenase deficiency, hemolytic disease, ionic strength
- MeSH terms
- Cations, Chlorides, Electric Conductivity, Erythrocytes, Hemolysis, Humans, Ion Channels, Osmolar Concentration, Oxidation-Reduction, Permeability, Reference Values, Patch-Clamp Techniques
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
