Presynaptic modulation by neuromedin U of sensory synaptic transmission in rat spinal dorsal horn neurones
Abstract
Neuromedin U (NMU) is a brain–gut peptide first isolated from the spinal cord. Recent studies on NMU and its receptors have suggested a role of NMU in sensory transmission. Here we report on the localization of NMU in sensory neurones, and the actions of NMU in the substantia gelatinosa (SG) and the deep layer of the dorsal horn (laminae III–V) in adult rat spinal cord slices using the patch‐clamp technique. An immunohistochemical study revealed that NMU peptide was present in most of the dorsal root ganglion neurones. In the spinal cord, NMU‐immunoreactive neurones were located in the deep layer (laminae III–V), but not in the SG. However, NMU‐positive axon terminals were observed in the SG as well as the deep layer. Bath‐applied NMU (10 μ m) increased the frequency, but not amplitude, of miniature excitatory postsynaptic currents (mEPSCs) in the SG and deep layer neurones by 146 ± 14% (P < 0.01, n= 17) and 174 ± 21% (P < 0.01, n= 6), respectively, without inducing any postsynaptic membrane currents recorded in tetrodotoxin. On the other hand, NMU did not affect miniature inhibitory postsynaptic currents recorded in tetrodotoxin. These findings, taken together, suggest that NMU acts on the presynaptic terminals of the primary afferent fibres working as an autocrine/paracrine neuromodulator to increase mEPSC frequency of the SG and deep layer neurones. This may account for the spinal mechanisms of the NMU‐induced hyperalgesia reported previously.
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The Sox5 dorsal excitatory family comprised Excit-14, Excit-15, Excit-16, Excit-17, Excit-18, and Excit-19 and expressed markers suggestive of a role in coping pain and mechanical nociception.
“The Sox5 dorsal, the excitatory family was comprised of Excit-14, Excit-15, Excit-16, Excit-17, Excit-18, and Excit-19, and expressed markers suggestive of a role in coping pain and mechanical nociception46,52,56,63–67.”
From A harmonized atlas of mouse spinal cord cell types and their spatial organization (2021) · cites this paper as [64]
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A harmonized atlas of mouse spinal cord cell types and their spatial organization
Russ, D. E., Cross, R. B. P., Li, L., Koch, S. C., Matson, K. J. E., Yadav, A., Alkaslasi, M. R., Lee, D. I., Le Pichon, C. E., Menon, V., et al. (2021) · Nature Communications · doi:10.1038/s41467-021-25125-1 · Cited by 255 · see citing works
- Authors
- Moriyama, M., Furue, H., Katafuchi, T., Teranishi, H., Sato, T., Kano, T., Kojima, M., & Yoshimura, M.
- Affiliations
- Kurume University (JP)
- Kyushu University (JP)
- Venue
- The Journal of Physiology
- Publisher
- Wiley
- Published
- 2004-09
- Volume
- 559
- Issue
- 3
- Pages
- 707-713
- ISSN
- 0022-3751 (ISSN-L)
- 1469-7793
- OpenAlex
- W2158618039
- Cited by
- 25
- References
- 13
- Citations by year
- 2025: 2 · 2024: 1 · 2023: 1 · 2022: 1 · 2021: 3 · 2020: 1 · 2019: 1 · 2016: 1 · 2015: 1 · 2012: 1
- FWCI
- 1.24
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- The Journal of Physiology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Physiology › Pain Mechanisms and Treatments
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuropeptides and Animal Physiology
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Keywords
- neuromedin U, presynaptic modulation, dorsal horn, substantia gelatinosa, dorsal root ganglia, primary afferent fibers, miniature excitatory postsynaptic currents, hyperalgesia
- MeSH terms
- Animals, Male, Synaptic Transmission, Neuropeptides, Rats, Sprague-Dawley, Rats, Wistar, Presynaptic Terminals, Posterior Horn Cells, Rats, In Vitro Techniques
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