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Cited by 67Ion Transport and Channel RegulationBarrier Structure and Function StudiesDrug Transport and Resistance Mechanisms

Transporters involved in regulation of intracellular pH in primary cultured rat brain endothelial cells

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Taylor, Caroline J., Nicola, Pieris A., Wang, Shanshan, Barrand, Margery A., Hladky, Stephen B.·2006·The Journal of Physiology
DOI 10.1113/jphysiol.2006.117374PMC PMC1890423
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Abstract

Fluid secretion across the blood-brain barrier, critical for maintaining the correct fluid balance in the brain, entails net secretion of HCO(3)(-), which is brought about by the combined activities of ion transporters situated in brain microvessels. These same transporters will concomitantly influence intracellular pH (pH(i)). To analyse the transporters that may be involved in the maintenance of pH(i) and hence secretion of HCO(3)(-), we have loaded primary cultured endothelial cells derived from rat brain microvessels with the pH indicator BCECF and suspended them in standard NaCl solutions buffered with Hepes or Hepes plus 5% CO(2)/HCO(3)(-). pH(i) in the standard solutions showed a slow acidification over at least 30 min, the rate being less in the presence of HCO(3)(-) than in its absence. However, after accounting for the difference in buffering, the net rates of acid loading with and without HCO(3)(-) were similar. In the nominal absence of HCO(3)(-) the rate of acid loading was increased equally by removal of external Na(+) or by inhibition of Na(+)/H(+) exchange by ethylisopropylamiloride (EIPA). By contrast, in the presence of HCO(3)(-) the increase in the rate of acid loading when Na(+) was removed was much larger and the rate was then also significantly greater than the rate observed in the absence of both Na(+) and HCO(3)(-). Removal of Cl(-) in the presence of HCO(3)(-) produced an alkalinization followed by a resumption of the slow acid gain. Removal of Na(+)…

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  • The blood-brain barrier has a major role in controlling the concentration of ions in the CNS, which is important for proper CNS functioning.

    “The BBB has a major role in controlling concentration of ions in the CNS, which is important for proper CNS functioning (582, 692).”

    From Blood-Brain Barrier: From Physiology to Disease and Back (2019) · cites this paper as [582]

  • The bicarbonate (HCO3-)-Cl- exchanger mediates entry of intracellular Cl- and the extracellular release of HCO3-.

    “The bicarbonate (HCO3 -)-Cl -exchanger mediates entry of intracellular Cl -and the extracellular release of HCO 3 -(582).”

    From Blood-Brain Barrier: From Physiology to Disease and Back (2019) · cites this paper as [582]

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Authors
Taylor, C. J., Nicola, P. A., Wang, S., Barrand, M. A., & Hladky, S. B.
Affiliations
University of Cambridge (GB)
ORCID
Venue
The Journal of Physiology
Publisher
Wiley
Published
2006-10-27
Volume
576
Issue
3
Pages
769-785
ISSN
  • 0022-3751 (ISSN-L)
  • 1469-7793
DOI
10.1113/jphysiol.2006.117374
PMC
PMC1890423
OpenAlex
W1849218644
Cited by
67
References
83
Citations by year
2025: 2 · 2024: 1 · 2023: 2 · 2022: 1 · 2021: 7 · 2020: 2 · 2019: 1 · 2018: 7 · 2017: 2 · 2016: 3 · 2015: 4 · 2014: 3 · 2013: 5 · 2012: 3
FWCI
2.31
Type
journal article
Language
en
Open access
Licence
onlinelibrary.wiley.com/termsAndConditions#vor (vor)
Locations
  • The Journal of Physiology (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Sir Jules Thorn Charitable Trust
  • Medical Research Council
  • Biotechnology and Biological Sciences Research Council (S19517)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ion Transport and Channel Regulation
  • Life Sciences › Neuroscience › Neurology › Barrier Structure and Function Studies
  • Health Sciences › Medicine › Oncology › Drug Transport and Resistance Mechanisms
Keywords
intracellular pH, brain microvascular endothelial cells, blood-brain barrier, bicarbonate secretion, Na+/HCO3- cotransporter, Na+/H+ exchanger, Cl-/HCO3- exchanger, acid load, DIDS, ethylisopropylamiloride, pH buffering, apical membrane
MeSH terms
Sodium-Potassium-Exchanging ATPase, Amiloride, Ammonium Chloride, Animals, Blood-Brain Barrier, Cells, Cultured, Endothelium, Vascular, Hydrogen-Ion Concentration, Mathematics, Rats, Wistar, 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid, Sodium-Hydrogen Exchangers, Neuroprotective Agents, Chloride-Bicarbonate Antiporters, Sodium-Bicarbonate Symporters, Sodium Chloride Symporters, Rats

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