Inhibition of initial bacterial adhesion on titanium surfaces by lactoferrin coating
Open accessAbstract
Because dental implant abutments are located at transmucosal sites, their surface should inhibit bacterial accumulation to prevent peri-implantitis. The authors examined the effects of human lactoferrin (LF), an antibacterial protein present in saliva, as an antibacterial coating on the titanium surface and evaluated its effects before and after mucin-containing artificial saliva (AS) incubation. In the control group, titanium disks were soaked in distilled water, whereas in the LF group, titanium disks were soaked in LF solution to coat the disks. In the control-AS and LF-AS groups, half of the control and LF disks were incubated with AS. To confirm LF adsorption, the fluorescence intensity of fluorescein isothiocyanate-labeled LF was measured. The LF and LF-AS groups showed significantly higher intensity than the control and control-AS groups (P 0.05). The amount of adhered Streptococcus gordonii significantly increased by incubation with AS (P < 0.01) and significantly decreased by adsorption of LF (P < 0.01). There was no interaction between the two factors, LF adsorption and AS incubation (P = 0.561). These results suggest that the adsorbed LF inhibited bacterial adhesion following AS incubation. According to qualitative LIVE/DEAD analysis, viable bacteria appeared to be decreased in the presence of LF and SEM observation indicated that altered morphologies increased in LF and LF-AS groups. These results suggest that the adsorbed LF remained on the titanium surface after…
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Ti implant surfaces modified with LF peptide show antimicrobial activity and prevent biofilm growth.
“These Ti implant surfaces modified with LF peptide showed antimicrobial activity by also preventing biofilm growth [53].”
From Strategies for Using Polydopamine to Induce Biomineralization of Hydroxyapatite on Implant Materials for Bone Tissue Engineering (2020) · cites this paper as [53]
Cites
Pjetursson, B. E., Thoma, D. S., Jung, R. E., Zwahlen, M., & Zembic, A. (2012) · Clinical Oral Implants Research · doi:10.1111/j.1600-0501.2012.02546.x · Cited by 953 · see citing works
Killing of gram-negative bacteria by lactoferrin and lysozyme.
Ellison, R. T., & Giehl, T. J. (1991) · Journal of Clinical Investigation · doi:10.1172/jci115407 · Cited by 744 · see citing works
Lactoferrin—a multifunctional protein with antimicrobial properties
Farnaud, S., & Evans, R. W. (2003) · Molecular Immunology · doi:10.1016/s0161-5890(03)00152-4 · Cited by 731 · see citing works
A Bactericidal Effect for Human Lactoferrin
Arnold, R. R., Cole, M. F., & McGhee, J. R. (1977) · Science · doi:10.1126/science.327545 · Cited by 677 · see citing works
Virulence factors of Porphyromonas gingivalis
Holt, S. C., Kesavalu, L. N., Walker, S. G., & Genco, C. A. (1999) · Periodontology 2000 · doi:10.1111/j.1600-0757.1999.tb00162.x · Cited by 644 · see citing works
Gifford, J. L., Hunter, H. N., & Vogel, H. J. (2005) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-005-5373-z · Cited by 529 · see citing works
Bacterial colonization immediately after installation on oral titanium implants
Fürst, M. M., Salvi, G. E., Lang, N. P., & Persson, G. R. (2007) · Clinical Oral Implants Research · doi:10.1111/j.1600-0501.2007.01381.x · Cited by 455 · see citing works
Biofilm on dental implants: a review of the literature
Subramani, K., Jung, R. E., Molenberg, A., & Hämmerle, C. H. F. (2009) · PubMed · doi:10.5167/uzh-26110 · Cited by 373 · see citing works
Papaspyridakos, P., Chen, C., Chuang, S.-K., Weber, H., & Gallucci, G. O. (2012) · PubMed · Cited by 333 · see citing works
Nibbering, P. H., Ravensbergen, E., Welling, M. M., van Berkel, L. A., van Berkel, P. H. C., Pauwels, E. K. J., & Nuijens, J. H. (2001) · Infection and Immunity · doi:10.1128/iai.69.3.1469-1476.2001 · Cited by 260 · see citing works
Periasamy, S., & Kolenbrander, P. E. (2009) · Journal of Bacteriology · doi:10.1128/jb.01006-09 · Cited by 226 · see citing works
Lamont, R. J., El-Sabaeny, A., Park, Y., Cook, G. S., Costerton, J. W., & Demuth, D. R. (2002) · Microbiology · doi:10.1099/00221287-148-6-1627 · Cited by 202 · see citing works
A review: The active peptide of lactoferrin
TOMITA, M., Takase, M., Bellamy, W., & Shimamura, S. (1994) · Pediatrics International · doi:10.1111/j.1442-200x.1994.tb03250.x · Cited by 180 · see citing works
A critical review of the roles of host lactoferrin in immunity
Legrand, D., & Mazurier, J. (2010) · BioMetals · doi:10.1007/s10534-010-9297-1 · Cited by 165 · see citing works
Porphyromonas gingivalis -Epithelial Cell Interactions in Periodontitis
Andrian, E., Grenier, D., & Rouabhia, M. (2006) · Journal of Dental Research · doi:10.1177/154405910608500502 · Cited by 159 · see citing works
Elucidation of the antistaphylococcal action of lactoferrin and lysozyme
Leitch, E. C., & Willcox, M. D. P. (1999) · Journal of Medical Microbiology · doi:10.1099/00222615-48-9-867 · Cited by 128 · see citing works
Legrand, D., Elass, E., Carpentier, M., & Mazurier, J. (2006) · Biochemistry and Cell Biology · doi:10.1139/o06-045 · Cited by 121 · see citing works
Brock, J. H. (2012) · Biochemistry and Cell Biology · doi:10.1139/o2012-018 · Cited by 115 · see citing works
Yoshinari, M., Kato, T., Matsuzaka, K., Hayakawa, T., & Shiba, K. (2009) · Biofouling · doi:10.1080/08927010903216572 · Cited by 112 · see citing works
Analysis of early biofilm formation on oral implants in man
Heuer, W., Elter, C., Demling, A. P., NEUMANN, A., Suerbaum, S., Hannig, M., Heidenblut, T., Bach, F. ‐., & Stiesch-Scholz, M. (2007) · Journal of Oral Rehabilitation · doi:10.1111/j.1365-2842.2007.01725.x · Cited by 100 · see citing works
Diarra, M. S., Petitclerc, D., & Lacasse, P. (2002) · Journal of Dairy Science · doi:10.3168/jds.s0022-0302(02)74176-3 · Cited by 86 · see citing works
Niemi, L. D., Hernell, O., & Johansson, I. (2009) · Caries Research · doi:10.1159/000213888 · Cited by 84 · see citing works
Huo, L., Zhang, K., Ling, J., Peng, Z., Huang, X., Liu, H., & Gu, L. (2011) · Archives of Oral Biology · doi:10.1016/j.archoralbio.2011.02.004 · Cited by 78 · see citing works
Jentsch, H. F. R., Sievert, Y., & Göcke, R. (2004) · Journal Of Clinical Periodontology · doi:10.1111/j.1600-051x.2004.00512.x · Cited by 75 · see citing works
The effect of lactoferrin on oral bacterial attachment
Arslan, S. Y., Leung, K. P., & Wu, C. D. (2009) · Oral Microbiology and Immunology · doi:10.1111/j.1399-302x.2009.00537.x · Cited by 72 · see citing works
Influence of saliva on aggregation and adherence of Streptococcus gordonii HG 222
Ligtenberg, A. J. M., Walgreen-Weterings, E., Veerman, E. C. I., de Soet, J. J., Graaff, J., & Amerongen, A. V. N. (1992) · Infection and Immunity · doi:10.1128/iai.60.9.3878-3884.1992 · Cited by 71 · see citing works
Mucosal inflammation and incidence of crestal bone loss among implant patients: a 10‐year study
Cecchinato, D., Parpaiola, A., & Lindhe, J. (2013) · Clinical Oral Implants Research · doi:10.1111/clr.12209 · Cited by 68 · see citing works
Microbiologically compromised patients and impact on oral implants
Quirynen, M., & Teughels, W. (2003) · Periodontology 2000 · doi:10.1046/j.0906-6713.2003.03310.x · Cited by 49 · see citing works
Rudney, J. D., Hickey, K. L., & Ji, Z. (1999) · Journal of Dental Research · doi:10.1177/00220345990780030801 · Cited by 49 · see citing works
Continuous Monitoring of pH and Eh in Bacterial Plaque Grown on a Tooth in an Artificial Mouth
Russell, C., & Coulter, W. A. (1975) · Applied Microbiology · doi:10.1128/am.29.2.141-144.1975 · Cited by 46 · see citing works
Chemical Modification of Metallic Implant Surfaces with Biofunctional Proteins
Endo, K. (1995) · Dental Materials Journal · doi:10.4012/dmj.14.199 · Cited by 44 · see citing works
Self-citationVisca, P., Berlutti, F., Vittorioso, P., Dalmastri, C., Thaller, M. C., & Valenti, P. (1989) · Medical Microbiology and Immunology · doi:10.1007/bf00203302 · Cited by 40 · see citing works
Effectiveness of cleaning surgical implants: Quantitative analysis of contaminant removal
Rowland, S. A., Shalaby, S. W., Latour, R. A., & von Recum, A. F. (1995) · Journal of Applied Biomaterials · doi:10.1002/jab.770060102 · Cited by 38 · see citing works
Characterizing the Adsorption of Peptides to TiO2 in Aqueous Solutions by Liquid Chromatography
Gertler, G., Fleminger, G., & Rapaport, H. (2010) · Langmuir · doi:10.1021/la903490v · Cited by 30 · see citing works
Länge, K., Herold, M., Scheideler, L., Geis‐Gerstorfer, J., Wendel, H.-P., & Gauglitz, G. (2004) · Dental Materials · doi:10.1016/j.dental.2003.10.010 · Cited by 29 · see citing works
Adhesion of oral streptococci to experimental bracket pellicles from glandular saliva
Ahn, S., Kho, H., Kim, K., & Nahm, D. (2003) · American Journal of Orthodontics and Dentofacial Orthopedics · doi:10.1016/s0889-5406(03)00346-9 · Cited by 28 · see citing works
DiPaola, C., & Mandel, I. D. (1980) · Journal of Dental Research · doi:10.1177/00220345800590090101 · Cited by 27 · see citing works
Mucin can enhance growth, biofilm formation, and survival ofStreptococcus mutans
Mothey, D., Buttaro, B. A., & Piggot, P. J. (2013) · FEMS Microbiology Letters · doi:10.1111/1574-6968.12336 · Cited by 25 · see citing works
Diarra, M. S. (2003) · Journal of Electron Microscopy · doi:10.1093/jmicro/52.2.207 · Cited by 16 · see citing works
Development of tissue conditioner capable of binding with anti-microbial protein lactoferrin
Yamamoto, D., Shinohara, Y., Nagadome, H., & Terada, Y. (2009) · Journal of Prosthodontic Research · doi:10.1016/j.jpor.2009.03.002 · Cited by 11 · see citing works
Enhanced compatibility of chemically modified titanium surface with periodontal ligament cells
Kado, T., Hidaka, T., Aita, H., Endo, K., & Furuichi, Y. (2012) · Applied Surface Science · doi:10.1016/j.apsusc.2012.07.091 · Cited by 9 · see citing works
Self-citationPark, J., Koh, M., Jang, Y., Choi, B., Kim, K., & Ko, Y. (2014) · Gerodontology · doi:10.1111/ger.12106 · Cited by 5 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Development of Antibiofilm Therapeutics Strategies to Overcome Antimicrobial Drug Resistance
Nadar, S., Khan, T. A., Patching, S. G., & Omri, A. (2022) · Microorganisms · doi:10.3390/microorganisms10020303 · Cited by 129 · see citing works
Hoyos‐Nogués, M., Velasco, F., Ginebra, M., Manero, J. M., Gil, F. J., & Mas‐Moruno, C. (2017) · ACS Applied Materials & Interfaces · doi:10.1021/acsami.7b03127 · Cited by 95 · see citing works
Kaushik, N., Nhat Nguyen, L., Kim, J. H., Choi, E. H., & Kumar Kaushik, N. (2020) · International Journal of Molecular Sciences · doi:10.3390/ijms21186544 · Cited by 76 · see citing works
“These Ti implant surfaces modified with LF peptide showed antimicrobial activity by also preventing biofilm growth [53].”
BackgroundHoyos‐Nogués, M., Buxadera‐Palomero, J., Ginebra, M., Manero, J. M., Gil, F. J., & Mas‐Moruno, C. (2018) · Colloids and Surfaces B Biointerfaces · doi:10.1016/j.colsurfb.2018.04.050 · Cited by 62 · see citing works
Lu, J., Francis, J. D., Guevara, M. A., Moore, R. E., Chambers, S. A., Doster, R. S., Eastman, A. J., Rogers, L. M., Noble, K. N., Manning, S. D., et al. (2021) · ChemBioChem · doi:10.1002/cbic.202100016 · Cited by 42 · see citing works
Microbial corrosion of metallic biomaterials in the oral environment
Xu, W., Yu, F., Addison, O., Zhang, B., Guan, F., Zhang, R., Hou, B., & Sand, W. (2024) · Acta Biomaterialia · doi:10.1016/j.actbio.2024.06.032 · Cited by 39 · see citing works
Ghilini, F., Fagali, N., Pissinis, D. E., Benitez, G. A., & Schilardi, P. L. (2021) · ACS Applied Bio Materials · doi:10.1021/acsabm.1c00613 · Cited by 38 · see citing works
Lactoferrin and oral pathologies: a therapeutic treatment
Rosa, L., Lepanto, M. S., Cutone, A., Ianiro, G., Pernarella, S., Sangermano, R., Musci, G., Ottolenghi, L., & Valenti, P. (2020) · Biochemistry and Cell Biology · doi:10.1139/bcb-2020-0052 · Cited by 32 · see citing works
Shen, T., Yang, W., Shen, X., Chen, W., Tao, B., Yang, X., Yuan, J., Liu, P., & Cai, K. (2018) · ACS Biomaterials Science & Engineering · doi:10.1021/acsbiomaterials.8b00791 · Cited by 30 · see citing works
Role of lactoferrin and lactoferrin‐derived peptides in oral and maxillofacial diseases
Velliyagounder, K., Bahdila, D., Pawar, S., & Fine, D. H. (2018) · Oral Diseases · doi:10.1111/odi.12868 · Cited by 27 · see citing works
Xu, X., Jin, T., Zhang, B., Liu, H., Ye, Z., Xu, Q., Chen, H., & Wang, B. (2016) · Polymer Testing · doi:10.1016/j.polymertesting.2016.12.006 · Cited by 25 · see citing works
Current Research in Pulsed Laser Deposition
Duta, L., & Popescu, A. C. (2021) · Coatings · doi:10.3390/coatings11030274 · Cited by 23 · see citing works
Effects of Bacterial Contamination on Dental Implants During Surgery
Johansson, K., Jimbo, R., Östlund, P., Tranæus, S. H., & Becktor, J. P. (2017) · Implant Dentistry · doi:10.1097/id.0000000000000660 · Cited by 21 · see citing works
Layer-by-layer self-assembly and clinical application in orthopedics
Ma, X., Zhao, D., Xiang, Y., Hua, Y., Zhao, W., Cui, Y., & Zhang, Z. (2022) · Journal of Material Science and Technology · doi:10.1016/j.jmst.2022.11.014 · Cited by 20 · see citing works
Brum, R. S., Monich, P. R., Fredel, M. C., Contri, G., Ramôa, S. D. A. S., Magini, R. S., & Benfatti, C. A. M. (2018) · Journal of Materials Science Materials in Medicine · doi:10.1007/s10856-018-6139-0 · Cited by 20 · see citing works
Lactoferrin Functionalized Biomaterials: Tools for Prevention of Implant-Associated Infections
Páll, E., & Roman, A. (2020) · Antibiotics · doi:10.3390/antibiotics9080522 · Cited by 17 · see citing works
Yoshida, E., & Hayakawa, T. (2016) · BioMed Research International · doi:10.1155/2016/3961286 · Cited by 16 · see citing works
Macrophage in vitro Response on Hybrid Coatings Obtained by Matrix Assisted Pulsed Laser Evaporation
Icriverzi, M., Rusen, L., Brajnicov, S., Bonciu, A. F., Dinescu, M., Cîmpean, A., Evans, R. W., Dinca, V. C., & Roșeanu, A. (2019) · Coatings · doi:10.3390/coatings9040236 · Cited by 15 · see citing works
Souza, J. G. S., Bertolini, M., Liu, J., NAGAY, B. E., Martins, R., Costa, R. C., Brunson, J. C., Shibli, J. A., Figueiredo, L. C., Dongari‐Bagtzoglou, A. I., et al. (2025) · ACS Nano · doi:10.1021/acsnano.5c06573 · Cited by 13 · see citing works
Overview on Adjunct Ingredients Used in Hydroxyapatite-Based Oral Care Products
Enax, J., Amaechi, B. T., Wiesche, E. S. Z., & Meyer, F. (2022) · Biomimetics · doi:10.3390/biomimetics7040250 · Cited by 13 · see citing works
Zhai, X., Ma, X., Myamina, M., Duan, J., & Hou, B. (2015) · Journal of Solid State Electrochemistry · doi:10.1007/s10008-015-2845-4 · Cited by 12 · see citing works
Plasma treatment for next-generation nanobiointerfaces
Levchenko, I., Keidar, M., Mai‐Prochnow, A., Modic, M., Cvelbar, U., Fang, J., & Ostrikov, K. (2015) · Biointerphases · doi:10.1116/1.4922237 · Cited by 10 · see citing works
Oral Cavity Calprotectin and Lactoferrin Levels in Relation to Radiotherapy
Keskin, M., Kompuinen, J., Harmankaya, İ., Karaçetin, D., Nissilä, V., Gürsoy, M., Sorsa, T. A., & Gürsoy, U. K. (2022) · Current Issues in Molecular Biology · doi:10.3390/cimb44100304 · Cited by 8 · see citing works
Shuto, T., Nakatani, T., Okamoto, K., Saizaki, N., Mimura, S., Kunitsugu, S., & Nikawa, H. (2016) · Journal of Photopolymer Science and Technology · doi:10.2494/photopolymer.29.413 · Cited by 7 · see citing works
Biological activation of zirconia surfaces by chemical modification with IGF‐1
Ito, D., Kado, T., Nagano‐Takebe, F., Hidaka, T., Endo, K., & Furuichi, Y. (2015) · Journal of Biomedical Materials Research Part A · doi:10.1002/jbm.a.35476 · Cited by 4 · see citing works
Self-citationImplant surface modifications and new development in surface coatings
Hintze, V., Bierbaum, S., & Scharnweber, D. (2019) · Elsevier eBooks · doi:10.1016/b978-0-08-102478-2.00005-2 · Cited by 4 · see citing works
Functionalization of titanium dental prostheses surface with antimicrobials GO and Cu2O
Rocha, A. M. L., Elias, C. N., Pinheiro, W. A., Guimarães, C. J. B., dos Anjos, V. D. C., Martinez, E. F., Lemos, A. B., & Corado, H. P. R. (2023) · Journal of Materials Research and Technology · doi:10.1016/j.jmrt.2023.06.063 · Cited by 2 · see citing works
Lactoferrin: a secret weapon in the war against pathogenic bacteria
Ruiz-Mazón, L., Rico, G. R., & de la Garza, M. (2024) · Exploration of Drug Science · doi:10.37349/eds.2024.00072 · Cited by 2 · see citing works
Osteogenic activity of lactoferrin and its application in contemporary dentistry
Trybek, G., Misiakiewicz-Has, K., Preuss, O., Szumilas, K., Szumilas, P., Aniko-Włodarczyk, M., & Grocholewicz, K. (2020) · Pomeranian Journal of Life Sciences · doi:10.21164/pomjlifesci.521 · Cited by 2 · see citing works
Antibiotic prophylaxis in dental implant surgery
Momand, P. (2025) · doi:10.24834/isbn.9789178776139
Yu, Y., Zhang, L., Xia, S., Toldi, G., Cornish, J., Huppertz, T., & Zhou, P. (2026) · Food Bioscience · doi:10.1016/j.fbio.2026.108441
- Authors
- Nagano-Takebe, F., Miyakawa, H., Nakazawa, F., & Endo, K.
- Affiliations
- School of Dentistry, Division of Biomaterials and Bioengineering, Health Sciences University of Hokkaido , 1757 Kanazawa, Ishikari-Tobetsu, Hokkaido 061-0293, Japan
- Health Sciences University of Hokkaido (JP)
- Venue
- Biointerphases
- Publisher
- American Vacuum Society
- Published
- 2014-03-05
- Volume
- 9
- Issue
- 2
- Pages
- 029006-029006
- ISSN
- 1559-4106 (ISSN-L)
- 1934-8630
- OpenAlex
- W2035777328
- Cited by
- 31
- References
- 42
- Citations by year
- 2026: 1 · 2025: 2 · 2024: 2 · 2023: 1 · 2022: 4 · 2021: 3 · 2020: 4 · 2019: 2 · 2018: 4 · 2017: 2 · 2016: 3 · 2015: 3
- FWCI
- 3.16
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Locations
- Biointerphases (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Funders
- Japan Society for the Promotion of Science (23792289)
- Topics
- Health Sciences › Dentistry › Oral Surgery › Dental Implant Techniques and Outcomes
- Health Sciences › Dentistry › Periodontics › Oral microbiology and periodontitis research
- Life Sciences › Immunology and Microbiology › Microbiology › Antimicrobial Peptides and Activities
- Keywords
- lactoferrin, titanium surfaces, implant abutments, bacterial adhesion inhibition, Streptococcus gordonii, peri-implantitis prevention, protein adsorption, bactericidal activity
- MeSH terms
- Adsorption, Bacterial Adhesion, Humans, Lactoferrin, Saliva, Saliva, Artificial, Surface Properties, Titanium, Dental Implants, Streptococcus gordonii
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
