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Cited by 21RNA Interference and Gene DeliveryPhotodynamic Therapy Research StudiesNanoparticle-Based Drug Delivery

Photochemical internalization-mediated nonviral gene transfection: polyamine core-shell nanoparticles as gene carrier

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Zamora, Genesis M., Wang, Frederick, Sun, Chung-Ho, Trinidad, Anthony, Kwon, Young Jik, Cho, Soo Kyung, Berg, Kristian, Madsen, Steen J. +1 more·2014·Journal of Biomedical Optics
DOI 10.1117/1.jbo.19.10.105009PMC PMC4206751
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Abstract

The overall objective of the research was to investigate the utility of photochemical internalization (PCI) for the enhanced nonviral transfection of genes into glioma cells. The PCI-mediated introduction of the tumor suppressor gene phosphatase and tensin homolog (PTEN) or the cytosine deaminase (CD) pro-drug activating gene into U87 or U251 glioma cell monolayers and multicell tumor spheroids were evaluated. In the study reported here, polyamine-DNA gene polyplexes were encapsulated in a nanoparticle (NP) with an acid degradable polyketal outer shell. These NP synthetically mimic the roles of viral capsid and envelope, which transport and release the gene, respectively. The effects of PCI-mediated suppressor and suicide genes transfection efficiency employing either “naked” polyplex cores alone or as NP-shelled cores were compared. PCI was performed with the photosensitizer AlPcS2a and λ=670-nm laser irradiance. The results clearly demonstrated that the PCI can enhance the delivery of both the PTEN or CD genes in human glioma cell monolayers and multicell tumor spheroids. The transfection efficiency, as measured by cell survival and inhibition of spheroid growth, was found to be significantly greater at suboptimal light and DNA levels for shelled NPs compared with polyamine-DNA polyplexes alone.

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Authors
Zamora, G. M., Wang, F., Sun, C.-H., Trinidad, A., Kwon, Y. J., Cho, S. K., Berg, K., Madsen, S. J., & Hirschberg, H.
Affiliations
  • University of California, Irvine (US)
  • Oslo University Hospital (NO)
  • University of Nevada, Las Vegas (US)
ORCID
Venue
Journal of Biomedical Optics
Publisher
SPIE-Intl Soc Optical Eng
Published
2014-10-23
Volume
19
Issue
10
Pages
1-8
ISSN
  • 1083-3668 (ISSN-L)
  • 1560-2281
DOI
10.1117/1.jbo.19.10.105009
PMC
PMC4206751
OpenAlex
W2169697985
Cited by
21
References
0
Citations by year
2023: 2 · 2021: 3 · 2020: 2 · 2019: 2 · 2018: 4 · 2017: 6 · 2015: 2
FWCI
1.09
Type
journal article
Language
en
Open access
Locations
  • Journal of Biomedical Optics (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
  • eScholarship (California Digital Library) (submittedVersion)
Funders
  • National Institutes of Health (P30CA062203)
  • National Cancer Institute of the National Institutes of Health (P30CA062203)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
  • Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Photodynamic Therapy Research Studies
  • Physical Sciences › Materials Science › Biomaterials › Nanoparticle-Based Drug Delivery
Keywords
photochemical internalization, glioma, PTEN, cytosine deaminase, suicide gene, tumor suppressor, polyplexes, gene delivery
MeSH terms
Cell Survival, Drug Carriers, Humans, Photochemotherapy, Polyamines, Transfection, Genetic Therapy, Photosensitizing Agents, Spheroids, Cellular, Cytosine Deaminase, Cell Line, Tumor, Cell Proliferation, PTEN Phosphohydrolase, Nanoparticles

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