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Reliability of Urinary Dehydration Markers in Elite Youth Boxers
Abstract
Purpose: To determine the reliability and diagnostic accuracy of noninvasive urinary dehydration markers in field-based settings on a day-to-day basis in elite adolescent amateur boxers. Methods: Sixty-nine urine samples were collected daily from 23 athletes (17.3 ± 1.9 y) during their weight-stable phase and analyzed by field and laboratory measures of hydration status. Urine osmolality (U OSM), urine specific gravity (U SG), total protein content (T PC), and body-mass stability were evaluated to determine fluid balance and hydration status. Overall macronutrient and water intake were determined using dietary records. According to their anthropometric characteristics, athletes were assigned into 2 groups: lightweight (L WB) and heavyweight (H WB) boxers. Results: Data presented on U OSM demonstrated a uniform increment by 11.2% ± 12.8% (L WB) and 19.9% ± 22.7% (H WB) (P < .001) over the course of the study, even during the weight-stable phase (body mass, ICC = .99) and ad libitum fluid intake (42 ± 4 mL · kg−1 · d−1). The intraclass correlation coefficients (ICCs) ranged from .52 to .55 for U SG and .38 to .52 for U OSM, further indicating inconsistency of the urinary dehydration markers. Poor correlations were found between U SG and T PC metabolites (r = .27, P = .211). Conclusions: Urinary dehydration markers (both U SG and U OSM) exhibit high variability and seem to be unreliable diagnostic tools to track actual body-weight loss in real-life settings. The ad libitum fluid intake was apparently inadequate to match acute fluid loss during and after intense preparation. The applicability of a single-time-point hydration-status assessment concept may preclude accurate assessment of actual body-weight deficits in youth boxers.
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“Although USG serves as a practical hydration marker, its variability and sensitivity to recent fluid intake [48] may influence single-time-point measurements.”
From Hydration and Fluid Intake in Basketball Players During Training: Comparison of Different Age Categories (2025) · cites this paper as [48]
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Citations
Cites
Exercise and Fluid Replacement
(2007) · Medicine & Science in Sports & Exercise · doi:10.1249/mss.0b013e31802ca597 · Cited by 1,497
Dehydration: Physiology, Assessment, and Performance Effects
Cheuvront, S. N., & Kenefick, R. W. (2014) · Comprehensive Physiology · doi:10.1002/cphy.c130017 · Cited by 461
Biological variation and diagnostic accuracy of dehydration assessment markers
Cheuvront, S. N., Ely, B. R., Kenefick, R. W., & Sawka, M. N. (2010) · The American Journal of Clinical Nutrition · doi:10.3945/ajcn.2010.29490 · Cited by 367
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- Akerman, 2016, Heat stress and dehydration in adapting for performance: good, bad, both, or neither?, Temperature, 3, 412, 10.1080/23328940.2016.1216255 · DOI
- Pettersson, 2014, Hydration status in elite wrestlers, judokas, boxers, and taekwondo athletes on competition day, Int J Sport Nutr Exerc Metab, 24, 267, 10.1123/ijsnem.2013-0100 · DOI · Paper page
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- Franchini, 2012, Weight loss in combat sports: physiological, psychological and performance effects, J Int Soc Sports Nutr, 9, 52, 10.1186/1550-2783-9-52 · DOI · Paper page
- Shirreffs, 1998, Urine osmolality and conductivity as indices of hydration status in athletes in the heat, Med Sci Sports Exerc, 30, 1598, 10.1097/00005768-199811000-00007 · DOI
- Artioli, 2010, Development, validity and reliability of a questionnaire designed to evaluate rapid weight loss patterns in judo players, Scand J Med Sci Sports, 20, e177, 10.1111/j.1600-0838.2009.00940.x · DOI
- Singh, 2013, Markers of hydration status in a 3-day trail running event, Clin J Sport Med, 23, 354, 10.1097/JSM.0b013e318286c2c3 · DOI
- Cheuvront, 2015, Spot urine concentrations should not used for hydration assessment: a methodology review, Int J Sport Nutr Exerc Metab, 25, 293, 10.1123/ijsnem.2014-0138 · DOI · Paper page
- Reljic, 2016, Rapid body mass loss affects erythropoiesis and hemolysis but does not impair aerobic performance in combat athletes, Scand J Med Sci Sports, 26, 507, 10.1111/sms.12485 · DOI
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- Gleim, 2000, Exercise and Sport Science
- Iwata, 1979, New micro-turbidimetric method for determination of protein in cerebrospinal fluid and urine, Clin Chem, 25, 1317, 10.1093/clinchem/25.7.1317 · DOI
- Fernandez-Elias, 2014, Validity of hydration non-invasive indices during the weight-cutting and official weigh-in for Olympic combat sports, PLoS ONE,, 9, 95336, 10.1371/journal.pone.0095336 · DOI
- Brown, 2012, Hydration status in adolescent judo athletes before and after training in the heat, Int J Sports Physiol Perform, 7, 39, 10.1123/ijspp.7.1.39 · DOI · Paper page
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- Carter, 1982, Physical structure of Olympic athletes, part I: the Montreal Olympic Games anthropological project, 16, 107
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- Reljic, 2015, Effects of pre-competitional rapid weight loss on nutrition, vitamin status and oxidative stress in elite boxers, J Sports Sci, 33, 437, 10.1080/02640414.2014.949825 · DOI
- Pettersson, 2014, Dietary intake at competition in elite Olympic combat sports, Int J Sport Nutr Exerc Metab, 24, 98, 10.1123/ijsnem.2013-0041 · DOI
- Adolph, 1947, Physiology of Man in the Desert
- Sawka, 2007, Exercise and fluid replacement, Med Sci Sports Exerc, 39, 377, 10.1249/mss.0b013e31802ca597 · DOI · Paper page
- Roemmich, 1997, Weight loss and wrestling training: effects on growth-related hormones, J Appl Physiol, 82, 1760, 10.1152/jappl.1997.82.6.1760 · DOI
- Hamouti, 2010, Effects of athletes’ muscle mass on urinary markers of hydration status, Eur J Appl Physiol, 109, 213, 10.1007/s00421-009-1333-x · DOI
- Galloway, 2000, The effects of substrate and fluid provision on thermoregulatory and metabolic responses to prolonged exercise in a hot environment, J Sports Sci, 2, 339, 10.1080/026404100402403 · DOI
Cited by
Kaplan, A., Ceylan, B., Baydil, B., & Šimenko, J. (2025) · Applied Sciences · doi:10.3390/app151910304
Metadata
- Authors
- Zubac, D., Cular, D., & Marusic, U.
- Venue
- International Journal of Sports Physiology and Performance
- Publisher
- Human Kinetics
- Published
- 2018-03-01
- Volume
- 13
- Issue
- 3
- Pages
- 374-381
- ISSN
- 1555-0265
- 1555-0273
- Cited by
- 11
- References
- 3
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