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Chrysin Ameliorates Chemically Induced Colitis in the Mouse through Modulation of a PXR/NF-κB Signaling Pathway
Open accessAbstract
Targeted activation of pregnane X receptor (PXR) in recent years has become a therapeutic strategy for inflammatory bowel disease. Chrysin is a naturally occurring flavonoid with anti-inflammation activity. The current study investigated the role of chrysin as a putative mouse PXR agonist in preventing experimental colitis. Pre-administration of chrysin ameliorated inflammatory symptoms in mouse models of colitis (dextran sodium sulfate- and 2,4,6-trinitrobenzene sulfonic acid-induced) and resulted in down-regulation of nuclear transcription factor κB (NF-κB) target genes (inducible NO synthase, intercellular adhesion molecule-1, monocyte chemotactic protein-1, cyclooxygenase 2, tumor necrosis factor-α, and interleukin 6) in the colon mucosa. Chrysin inhibited the phosphorylation/degradation of inhibitor κBα (IκBα), which correlated with the decrease in the activity of myeloperoxidase and the levels of tumor necrosis factor-α and interleukin 6 in the colon. Consistent with the in vivo results, chrysin blocked lipopolysaccharide -stimulated nuclear translocation of NF-κB p65 in mouse macrophage RAW264.7. Furthermore, chrysin dose-dependently activated human/mouse PXR in reporter gene assays and up-regulated xenobiotic detoxification genes in the colon mucosa, but not in the liver. Silencing of PXR by RNA interference demonstrated necessity of PXR in mediating chrysin's ability to induce xenobiotic detoxification genes and NF-κB inactivation. The repression of NF-κB transcription activity by chrysin was confirmed by in vitro PXR transduction. These findings suggest that the effect of chrysin in preventing chemically induced colitis is mediated in large part by a PXR/NF-κB pathway. The data also suggest that chrysin or chrysin-like flavonoids could be further developed as intestine-specific PXR activators.
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“administration of synthetic NR1I2 agonists exerts protective effects in mouse models of colitis (Dou et al., 2013; Garg et al., 2016; Shah et al., 2007; Terc et al., 2014; Venkatesh et al., 2014).”
From Understanding the Holobiont: How Microbial Metabolites Affect Human Health and Shape the Immune System (2017) · cites this paper as Dou et al., 2013
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“Nr1i2−/− mice exhibit impaired epithelial barrier function”
From Understanding the Holobiont: How Microbial Metabolites Affect Human Health and Shape the Immune System (2017) · cites this paper as Dou et al., 2013
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Cites
Okayasu, I., Hatakeyama, S., Yamada, M., Ohkusa, T., Inagaki, Y., & NAKAYA, R. (1990) · Gastroenterology · doi:10.1016/0016-5085(90)90290-h · Cited by 2,408
Alleviating Cancer Drug Toxicity by Inhibiting a Bacterial Enzyme
Wallace, B. D., Wang, H., Lane, K. T., Scott, J. E., Orans, J., Koo, J. S., Venkatesh, M., Jobin, C., Yeh, L.-A., Mani, S., et al. (2010) · Science · doi:10.1126/science.1191175 · Cited by 1,080
Self-citationPanwala, C. M., Jones, J. C., & Viney, J. L. (1998) · The Journal of Immunology · doi:10.4049/jimmunol.161.10.5733 · Cited by 567
IL-1β-converting enzyme (caspase-1) in intestinal inflammation
Siegmund, B., Lehr, H.-A., Fantuzzi, G., & Dinarello, C. A. (2001) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.231473998 · Cited by 421
Zhou, C. (2006) · Journal of Clinical Investigation · doi:10.1172/jci26283 · Cited by 404
Langmann, T., Moehle, C., Mauerer, R., Scharl, M. M., Liebisch, G., Zahn, A., Stremmel, W., & Schmitz, G. (2004) · Gastroenterology · doi:10.1053/j.gastro.2004.04.019 · Cited by 335
Contreras, O. M., Geem, D., Laur, O., Williams, I. R., Lira, S. A., Nusrat, A., Parkos, C. A., & Denning, T. L. (2011) · Journal of Clinical Investigation · doi:10.1172/jci59150 · Cited by 333
Role of NF-κB in Regulation of PXR-mediated Gene Expression
Gu, X., Ke, S., Liu, D., Sheng, T., Thomas, P. E., Rabson, A. B., Gallo, M. A., Xie, W., & Tian, Y. (2006) · Journal of Biological Chemistry · doi:10.1074/jbc.m601302200 · Cited by 312
Disposition and metabolism of the flavonoid chrysin in normal volunteers
Walle, T., Otake, Y., Brubaker, J., Walle, U. K., & Halushka, P. V. (2001) · British Journal of Clinical Pharmacology · doi:10.1111/j.1365-2125.2001.01317.x · Cited by 295
Shah, Y. M., Ma, X., Morimura, K., Kim, I., & Gonzalez, F. J. (2007) · American Journal of Physiology-Gastrointestinal and Liver Physiology · doi:10.1152/ajpgi.00528.2006 · Cited by 231
Chen, Y., Tang, Y., Guo, C., Wang, J., Boral, D., & Nie, D. (2012) · Biochemical Pharmacology · doi:10.1016/j.bcp.2012.01.030 · Cited by 227
Adachi, M., Kurotani, R., Morimura, K., Shah, Y. M., Sanford, M., Madison, B. B., Gumucio, D. L., Marin, H. E., Peters, J. M., Young, H. A., et al. (2006) · Gut · doi:10.1136/gut.2005.081745 · Cited by 211
The Pregnane X Receptor Locus Is Associated With Susceptibility to Inflammatory Bowel Disease
Dring, M. M., Goulding, C., Trimble, V., Keegan, D., Ryan, A. W., Brophy, K., Smyth, C. M., Keeling, P. W. N., O’Donoghue, D., OʼSullivan, M., et al. (2006) · Gastroenterology · doi:10.1053/j.gastro.2005.12.008 · Cited by 165
Cheng, J., Shah, Y. M., Ma, X., Pang, X., Tanaka, T., Kodama, T., Krausz, K. W., & Gonzalez, F. J. (2010) · Journal of Pharmacology and Experimental Therapeutics · doi:10.1124/jpet.110.170225 · Cited by 162
Woo, K. J., Jeong, Y., Inoue, H., Park, J., & Kwon, T. K. (2005) · FEBS Letters · doi:10.1016/j.febslet.2004.12.048 · Cited by 155
Blokzijl, H., Borght, S. V., Bok, L. I. H., Libbrecht, L. J., Geuken, M., van den Heuvel, F. A. J., Dijkstra, G. D. H., Roskams, T., Moshage, H. J., Jansen, P. L. M., et al. (2007) · Inflammatory Bowel Diseases · doi:10.1002/ibd.20088 · Cited by 143
Rifaximin Is a Gut-Specific Human Pregnane X Receptor Activator
Ma, X., Shah, Y. M., Guo, G. L., Wang, T., Krausz, K. W., Idle, J. R., & Gonzalez, F. J. (2007) · Journal of Pharmacology and Experimental Therapeutics · doi:10.1124/jpet.107.121913 · Cited by 128
Chrysin, a natural flavone, improves murine inflammatory bowel diseases
Shin, E. K., Kwon, H.-S., Kim, Y. H., Shin, H., & Kim, J. (2009) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2009.02.071 · Cited by 109
Sugatani, J., Yamakawa, K., Tonda, E., Nishitani, S., Yoshinari, K., Degawa, M., Abe, I., Noguchi, H., & Miwa, M. (2003) · Biochemical Pharmacology · doi:10.1016/j.bcp.2003.11.002 · Cited by 107
Mencarelli, A., Migliorati, M., Barbanti, M., Cipriani, S., Palladino, G., Distrutti, E., Renga, B., & Fiorucci, S. (2010) · Biochemical Pharmacology · doi:10.1016/j.bcp.2010.08.022 · Cited by 103
Alleviation of Gut Inflammation by Cdx2/Pxr Pathway in a Mouse Model of Chemical Colitis
Dou, W., Mukherjee, S., Li, H., Venkatesh, M., Wang, H., Kortagere, S., Peleg, A., Chilimuri, S. S., Wang, Z., Feng, Y., et al. (2012) · PLoS ONE · doi:10.1371/journal.pone.0036075 · Cited by 92
Self-citationZhang, D. K., Cheng, L. N., Huang, X. L., Shi, W., Xiang, J. Y., & Gan, H. T. (2008) · International Journal of Colorectal Disease · doi:10.1007/s00384-008-0544-7 · Cited by 82
Xenobiotic receptor meets NF-κB, a collision in the small bowel
Xie, W., & Tian, Y. (2006) · Cell Metabolism · doi:10.1016/j.cmet.2006.08.004 · Cited by 68
Shinoda, M., Shin‐Ya, M., Naito, Y., Kishida, T., Ito, R., Suzuki, N., Yasuda, H., Sakagami, J., Imanishi, J., Kataoka, K., et al. (2010) · Journal of Gastroenterology · doi:10.1007/s00535-010-0210-z · Cited by 61
Development of FXR, PXR and CAR Agonists and Antagonists for Treatment of Liver Disorders
Fiorucci, S., Zampella, A., & Distrutti, E. (2012) · Current Topics in Medicinal Chemistry · doi:10.2174/156802612799436678 · Cited by 51
Coordinated defence and the liver
Elias, E., & Mills, C. O. (2007) · Clinical Medicine · doi:10.7861/clinmedicine.7-2-180 · Cited by 21
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Postler, T. S., & Ghosh, S. (2017) · Cell Metabolism · doi:10.1016/j.cmet.2017.05.008 · Cited by 876
“Nr1i2 / mice exhibit impaired epithelial barrier function, and administration of synthetic NR1I2 agonists exerts protective effects in mouse models of colitis (Dou et al., 2013; Garg et al., 2016; Shah et al., 2007; Terc et al., 2014; Venkatesh et al., 2014).”
BackgroundMantawy, E. M., El‐Bakly, W. M., Esmat, A., Badr, A. M., & El‐Demerdash, E. (2014) · European Journal of Pharmacology · doi:10.1016/j.ejphar.2014.01.065 · Cited by 259
Flavonoids in Inflammatory Bowel Disease: A Review
Vezza, T., Rodríguez‐Nogales, A., Algieri, F., Utrilla, M. P., Rodríguez‐Cabezas, M. E., & Gálvez, J. (2016) · Nutrients · doi:10.3390/nu8040211 · Cited by 247
Zeinali, M., Rezaee, S. A., & Hosseinzadeh, H. (2017) · Biomedicine & Pharmacotherapy · doi:10.1016/j.biopha.2017.06.003 · Cited by 225
Wang, K., Jin, X., Li, Q., Sawaya, A. C. H. F., Leu, R. K. L., Conlon, M. A., Wu, L., & Hu, F. (2018) · Molecular Nutrition & Food Research · doi:10.1002/mnfr.201800080 · Cited by 204
Metadata
- Authors
- Dou, W., Zhang, J., Zhang, E., Sun, A., Ding, L., Chou, G., Wang, Z., & Mani, S.
- Affiliations
- Shanghai University of Traditional Chinese Medicine (CN)
- Albert Einstein College of Medicine (US)
- Venue
- The Journal of Pharmacology and Experimental Therapeutics
- Publisher
- Elsevier BV
- Published
- 2013-06
- Volume
- 345
- Issue
- 3
- Pages
- 473-482
- ISSN
- 0022-3565 (ISSN-L)
- PMC
- PMC3657113
- OpenAlex
- W2143953127
- Cited by
- 111
- References
- 26
- Citations by year
- 2026: 5 · 2025: 2 · 2024: 7 · 2023: 7 · 2022: 8 · 2021: 9 · 2020: 7 · 2019: 10 · 2018: 8 · 2017: 17 · 2016: 12 · 2015: 6 · 2014: 11 · 2013: 2
- FWCI
- 7.59
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Journal of Pharmacology and Experimental Therapeutics (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Cancer Institute (R01CA161879, R01CA127231)
- Topics
- Health Sciences › Medicine › Pharmacology (medical) › Flavonoids in Medical Research
- Health Sciences › Medicine › Gastroenterology › Inflammatory Bowel Disease
- Health Sciences › Medicine › Complementary and alternative medicine › Natural Compounds in Disease Treatment
- Keywords
- chrysin, pregnane X receptor, NF-κB signaling, experimental colitis, inflammatory bowel disease, DSS-induced colitis, trinitrobenzene sulfonic acid-induced colitis, flavonoids, IκBα phosphorylation, TNF-α, interleukin-6, macrophages, colonic mucosa, PXR agonists
- MeSH terms
- Pregnane X Receptor, Animals, Colitis, Female, Flavonoids, Fluorescent Antibody Technique, Humans, Mice, Inbred C57BL, Peroxidase, Receptors, Steroid, RNA, Trinitrobenzenesulfonic Acid, Tumor Necrosis Factor-alpha, Blotting, Western, Signal Transduction, Interleukin-6, Up-Regulation, Dextran Sulfate, NF-kappa B, Genes, Reporter, Gene Silencing, Mice
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