The DOI matches its Crossref record; 26 of the 26 references it cites were found in a registry or an index.
Chrysin Ameliorates Chemically Induced Colitis in the Mouse through Modulation of a PXR/NF-κB Signaling Pathway
Open accessAbstract
Targeted activation of pregnane X receptor (PXR) in recent years has become a therapeutic strategy for inflammatory bowel disease. Chrysin is a naturally occurring flavonoid with anti-inflammation activity. The current study investigated the role of chrysin as a putative mouse PXR agonist in preventing experimental colitis. Pre-administration of chrysin ameliorated inflammatory symptoms in mouse models of colitis (dextran sodium sulfate- and 2,4,6-trinitrobenzene sulfonic acid-induced) and resulted in down-regulation of nuclear transcription factor κB (NF-κB) target genes (inducible NO synthase, intercellular adhesion molecule-1, monocyte chemotactic protein-1, cyclooxygenase 2, tumor necrosis factor-α, and interleukin 6) in the colon mucosa. Chrysin inhibited the phosphorylation/degradation of inhibitor κBα (IκBα), which correlated with the decrease in the activity of myeloperoxidase and the levels of tumor necrosis factor-α and interleukin 6 in the colon. Consistent with the in vivo results, chrysin blocked lipopolysaccharide -stimulated nuclear translocation of NF-κB p65 in mouse macrophage RAW264.7. Furthermore, chrysin dose-dependently activated human/mouse PXR in reporter gene assays and up-regulated xenobiotic detoxification genes in the colon mucosa, but not in the liver. Silencing of PXR by RNA interference demonstrated necessity of PXR in mediating chrysin's ability to induce xenobiotic detoxification genes and NF-κB inactivation. The repression of NF-κB transcription activity by chrysin was confirmed by in vitro PXR transduction. These findings suggest that the effect of chrysin in preventing chemically induced colitis is mediated in large part by a PXR/NF-κB pathway. The data also suggest that chrysin or chrysin-like flavonoids could be further developed as intestine-specific PXR activators.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In the citing paper
“Nr1i2−/− mice exhibit impaired epithelial barrier function”
From Understanding the Holobiont: How Microbial Metabolites Affect Human Health and Shape the Immune System (2017) · cites this paper as Dou et al., 2013
In the citing paper
“administration of synthetic NR1I2 agonists exerts protective effects in mouse models of colitis (Dou et al., 2013; Garg et al., 2016; Shah et al., 2007; Terc et al., 2014; Venkatesh et al., 2014).”
From Understanding the Holobiont: How Microbial Metabolites Affect Human Health and Shape the Immune System (2017) · cites this paper as Dou et al., 2013
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Okayasu, I., Hatakeyama, S., Yamada, M., Ohkusa, T., Inagaki, Y., & NAKAYA, R. (1990) · Gastroenterology · doi:10.1016/0016-5085(90)90290-h · Cited by 2,408
Alleviating Cancer Drug Toxicity by Inhibiting a Bacterial Enzyme
Wallace, B. D., Wang, H., Lane, K. T., Scott, J. E., Orans, J., Koo, J. S., Venkatesh, M., Jobin, C., Yeh, L.-A., Mani, S., et al. (2010) · Science · doi:10.1126/science.1191175 · Cited by 1,080
Self-citationPanwala, C. M., Jones, J. C., & Viney, J. L. (1998) · The Journal of Immunology · doi:10.4049/jimmunol.161.10.5733 · Cited by 567
IL-1β-converting enzyme (caspase-1) in intestinal inflammation
Siegmund, B., Lehr, H.-A., Fantuzzi, G., & Dinarello, C. A. (2001) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.231473998 · Cited by 421
Zhou, C. (2006) · Journal of Clinical Investigation · doi:10.1172/jci26283 · Cited by 404
Langmann, T., Moehle, C., Mauerer, R., Scharl, M. M., Liebisch, G., Zahn, A., Stremmel, W., & Schmitz, G. (2004) · Gastroenterology · doi:10.1053/j.gastro.2004.04.019 · Cited by 335
Contreras, O. M., Geem, D., Laur, O., Williams, I. R., Lira, S. A., Nusrat, A., Parkos, C. A., & Denning, T. L. (2011) · Journal of Clinical Investigation · doi:10.1172/jci59150 · Cited by 333
Role of NF-κB in Regulation of PXR-mediated Gene Expression
Gu, X., Ke, S., Liu, D., Sheng, T., Thomas, P. E., Rabson, A. B., Gallo, M. A., Xie, W., & Tian, Y. (2006) · Journal of Biological Chemistry · doi:10.1074/jbc.m601302200 · Cited by 312
Disposition and metabolism of the flavonoid chrysin in normal volunteers
Walle, T., Otake, Y., Brubaker, J., Walle, U. K., & Halushka, P. V. (2001) · British Journal of Clinical Pharmacology · doi:10.1111/j.1365-2125.2001.01317.x · Cited by 295
Shah, Y. M., Ma, X., Morimura, K., Kim, I., & Gonzalez, F. J. (2007) · American Journal of Physiology-Gastrointestinal and Liver Physiology · doi:10.1152/ajpgi.00528.2006 · Cited by 231
Cited by
Sorted by citations
Postler, T. S., & Ghosh, S. (2017) · Cell Metabolism · doi:10.1016/j.cmet.2017.05.008 · Cited by 876
“Nr1i2 / mice exhibit impaired epithelial barrier function, and administration of synthetic NR1I2 agonists exerts protective effects in mouse models of colitis (Dou et al., 2013; Garg et al., 2016; Shah et al., 2007; Terc et al., 2014; Venkatesh et al., 2014).”
BackgroundMantawy, E. M., El‐Bakly, W. M., Esmat, A., Badr, A. M., & El‐Demerdash, E. (2014) · European Journal of Pharmacology · doi:10.1016/j.ejphar.2014.01.065 · Cited by 259
Flavonoids in Inflammatory Bowel Disease: A Review
Vezza, T., Rodríguez‐Nogales, A., Algieri, F., Utrilla, M. P., Rodríguez‐Cabezas, M. E., & Gálvez, J. (2016) · Nutrients · doi:10.3390/nu8040211 · Cited by 247
Zeinali, M., Rezaee, S. A., & Hosseinzadeh, H. (2017) · Biomedicine & Pharmacotherapy · doi:10.1016/j.biopha.2017.06.003 · Cited by 225
Wang, K., Jin, X., Li, Q., Sawaya, A. C. H. F., Leu, R. K. L., Conlon, M. A., Wu, L., & Hu, F. (2018) · Molecular Nutrition & Food Research · doi:10.1002/mnfr.201800080 · Cited by 204
Metadata
- Authors
- Dou, W., Zhang, J., Zhang, E., Sun, A., Ding, L., Chou, G., Wang, Z., & Mani, S.
- Affiliations
- Shanghai University of Traditional Chinese Medicine (CN)
- Albert Einstein College of Medicine (US)
- Venue
- The Journal of Pharmacology and Experimental Therapeutics
- Publisher
- Elsevier BV
- Published
- 2013-06
- Volume
- 345
- Issue
- 3
- Pages
- 473-482
- ISSN
- 0022-3565 (ISSN-L)
- PMC
- PMC3657113
- OpenAlex
- W2143953127
- Cited by
- 111
- References
- 26
- Citations by year
- 2026: 5 · 2025: 2 · 2024: 7 · 2023: 7 · 2022: 8 · 2021: 9 · 2020: 7 · 2019: 10 · 2018: 8 · 2017: 17 · 2016: 12 · 2015: 6 · 2014: 11 · 2013: 2
- FWCI
- 7.59
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Journal of Pharmacology and Experimental Therapeutics (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Cancer Institute (R01CA161879, R01CA127231)
- Topics
- Health Sciences › Medicine › Pharmacology (medical) › Flavonoids in Medical Research
- Health Sciences › Medicine › Gastroenterology › Inflammatory Bowel Disease
- Health Sciences › Medicine › Complementary and alternative medicine › Natural Compounds in Disease Treatment
- Keywords
- chrysin, pregnane X receptor, NF-κB signaling, experimental colitis, inflammatory bowel disease, DSS-induced colitis, trinitrobenzene sulfonic acid-induced colitis, flavonoids, IκBα phosphorylation, TNF-α, interleukin-6, macrophages, colonic mucosa, PXR agonists
- MeSH terms
- Pregnane X Receptor, Animals, Colitis, Female, Flavonoids, Fluorescent Antibody Technique, Humans, Mice, Inbred C57BL, Peroxidase, Receptors, Steroid, RNA, Trinitrobenzenesulfonic Acid, Tumor Necrosis Factor-alpha, Blotting, Western, Signal Transduction, Interleukin-6, Up-Regulation, Dextran Sulfate, NF-kappa B, Genes, Reporter, Gene Silencing, Mice
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
