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Ca2+/Calmodulin-Dependent Kinase (CaMK) Signaling via CaMKI and AMP-Activated Protein Kinase Contributes to the Regulation of WIPI-1 at the Onset of Autophagy

Pfisterer, Simon G., Mauthe, Mario, Codogno, Patrice, Proikas-Cezanne, Tassula·2011·Molecular Pharmacology
DOI 10.1124/mol.111.071761
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Abstract

Autophagy is initiated by multimembrane vesicle (autophagosome) formation upon mammalian target of rapamycin inhibition and phosphatidylinositol 3-phosphate [PtdIns(3)P] generation. Upstream of microtubule-associated protein 1 light chain 3 (LC3), WD-repeat proteins interacting with phosphoinositides (WIPI proteins) specifically bind PtdIns(3)P at forming autophagosomal membranes and become membrane-bound proteins of generated autophagosomes. Here, we applied automated high-throughput WIPI-1 puncta analysis, paralleled with LC3 lipidation assays, to investigate Ca(2+)-mediated autophagy modulation. We imposed cellular stress by starvation or administration of etoposide (0.5-50 μM), sorafenib (1-40 μM), staurosporine (20-500 nM), or thapsigargin (20-500 nM) (1, 2, or 3 h) and measured the formation of WIPI-1 positive autophagosomal membranes. Automated analysis of up to 5000 individual cells/treatment demonstrated that Ca(2+) chelation by BAPTA-AM (10 and 30 μM) counteracted starvation or pharmacological compound-induced WIPI-1 puncta formation and LC3 lipidation. Application of selective Ca(2+)/calmodulin-dependent kinase kinase (CaMKK) α/β and calmodulin-dependent kinase (CaMK) I/II/IV inhibitors 7-oxo-7H-benzimidazo[2,1-a]benz[de]isoquinoline-3-carboxylic acid acetate (STO-609; 10-30 μg/ml) and 2-(N-[2-hydroxyethyl])-N-(4-methoxybenzenesulfonyl)amino-N-(4-chlorocinnamyl)-N-methylamine (KN-93; 1-10 μM), respectively, significantly reduced starvation-induced autophagosomal me…

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Research works printed but not linked: 35

  1. Barrett, 2008, Genome-wide association defines more than 30 distinct susceptibility loci for Crohn’s disease, Nat Genet, 40, 955, 10.1038/ng.175 · DOI · Paper page
  2. Blommaart, 1997, The phosphatidylinositol 3-kinase inhibitors wortmannin and LY294002 inhibit autophagy in isolated rat hepatocytes, Eur J Biochem, 243, 240, 10.1111/j.1432-1033.1997.0240a.x · DOI
  3. Blommaart, 1995, Phosphorylation of ribosomal protein S6 is inhibitory for autophagy in isolated rat hepatocytes, J Biol Chem, 270, 2320, 10.1074/jbc.270.5.2320 · DOI · Paper page
  4. Brady, 2007, The autophagic response to nutrient deprivation in the hl-1 cardiac myocyte is modulated by Bcl-2 and sarco/endoplasmic reticulum calcium stores, FEBS J, 274, 3184, 10.1111/j.1742-4658.2007.05849.x · DOI · Paper page
  5. Chen, 2011, The regulation of autophagy - unanswered questions, J Cell Sci, 124, 161, 10.1242/jcs.064576 · DOI
  6. Christensen, 1998, Staurosporine-induced cell death in Tetrahymena thermophila has mixed characteristics of both apoptotic and autophagic degeneration, Cell Biol Int, 22, 591, 10.1006/cbir.1998.0320 · DOI
  7. Codogno, 1997, Signal transduction pathways in macroautophagy, Cell Signal, 9, 125, 10.1016/S0898-6568(96)00130-1 · DOI
  8. Dale, 1995, Similar substrate recognition motifs for mammalian AMP-activated protein kinase, higher plant HMG-CoA reductase kinase-A, yeast SNF1, and mammalian calmodulin-dependent protein kinase I, FEBS Lett, 361, 191, 10.1016/0014-5793(95)00172-6 · DOI
  9. Egan, 2011, Phosphorylation of ULK1 (hATG1) by AMP-activated protein kinase connects energy sensing to mitophagy, Science, 331, 456, 10.1126/science.1196371 · DOI · Paper page
  10. Fleming, 2011, Chemical modulators of autophagy as biological probes and potential therapeutics, Nat Chem Biol, 7, 9, 10.1038/nchembio.500 · DOI
  11. Grotemeier, 2010, AMPK-independent induction of autophagy by cytosolic Ca2+ increase, Cell Signal, 22, 914, 10.1016/j.cellsig.2010.01.015 · DOI · Paper page
  12. Hardie, 2008, Role of AMP-activated protein kinase in the metabolic syndrome and in heart disease, FEBS Lett, 582, 81, 10.1016/j.febslet.2007.11.018 · DOI
  13. Holen, 1992, Protein kinase-dependent effects of okadaic acid on hepatocytic autophagy and cytoskeletal integrity, Biochem J, 284, 633, 10.1042/bj2840633 · DOI
  14. Høyer-Hansen, 2007, Control of macroautophagy by calcium, calmodulin-dependent kinase kinase-beta, and Bcl-2, Mol Cell, 25, 193, 10.1016/j.molcel.2006.12.009 · DOI · Paper page
  15. Itakura, 2010, Characterization of autophagosome formation site by a hierarchical analysis of mammalian Atg proteins, Autophagy, 6, 764, 10.4161/auto.6.6.12709 · DOI · Paper page
  16. Khan, 2010, Role of inositol trisphosphate receptors in autophagy in DT40 cells, J Biol Chem, 285, 16912, 10.1074/jbc.M110.114207 · DOI
  17. Kim, 2011, AMPK and mTOR regulate autophagy through direct phosphorylation of Ulk1, Nat Cell Biol, 13, 132, 10.1038/ncb2152 · DOI · Paper page
  18. Klionsky, 2007, How shall I eat thee?, Autophagy, 3, 413, 10.4161/auto.4377 · DOI
  19. Klionsky, 2000, Autophagy as a regulated pathway of cellular degradation, Science, 290, 1717, 10.1126/science.290.5497.1717 · DOI
  20. Kondo, 2005, The role of autophagy in cancer development and response to therapy, Nat Rev Cancer, 5, 726, 10.1038/nrc1692 · DOI
  21. Laderoute, 2006, 5′-AMP-activated protein kinase (AMPK) is induced by low-oxygen and glucose deprivation conditions found in solid-tumor microenvironments, Mol Cell Biol, 26, 5336, 10.1128/MCB.00166-06 · DOI
  22. Liang, 1999, Induction of autophagy and inhibition of tumorigenesis by beclin 1, Nature, 402, 672, 10.1038/45257 · DOI · Paper page
  23. Means, 2008, The Year in Basic Science: calmodulin kinase cascades, Mol Endocrinol, 22, 2759, 10.1210/me.2008-0312 · DOI
  24. Mizushima, 2008, Autophagy fights disease through cellular self-digestion, Nature, 451, 1069, 10.1038/nature06639 · DOI · Paper page
  25. Nakatogawa, 2007, Atg8, a ubiquitin-like protein required for autophagosome formation, mediates membrane tethering and hemifusion, Cell, 130, 165, 10.1016/j.cell.2007.05.021 · DOI
  26. Nishida, 2009, Discovery of Atg5/Atg7-independent alternative macroautophagy, Nature, 461, 654, 10.1038/nature08455 · DOI · Paper page
  27. Nobukuni, 2007, hvps34, an ancient player, enters a growing game: mTOR Complex1/S6K1 signaling, Curr Opin Cell Biol, 19, 135, 10.1016/j.ceb.2007.02.019 · DOI
  28. Noda, 2010, Regulation of membrane biogenesis in autophagy via PI3P dynamics, Semin Cell Dev Biol, 21, 671, 10.1016/j.semcdb.2010.04.002 · DOI
  29. Park, 2008, Vorinostat and sorafenib increase ER stress, autophagy and apoptosis via ceramide-dependent CD95 and PERK activation, Cancer Biol Ther, 7, 1648, 10.4161/cbt.7.10.6623 · DOI
  30. Petiot, 2000, Distinct classes of phosphatidylinositol 3′-kinases are involved in signaling pathways that control macroautophagy in HT-29 cells, J Biol Chem, 275, 992, 10.1074/jbc.275.2.992 · DOI
  31. Proikas-Cezanne, 2011, Beclin 1 or not Beclin 1…, Autophagy, 7, 671, 10.4161/auto.7.7.14877 · DOI
  32. Proikas-Cezanne T, Robenek H, (2011) Freeze-fracture replica immunolabelling reveals human WIPI-1 and WIPI-2 as membrane proteins of autophagosomes. J Cell Mol Med doi:10.1111/j.1582-4934.2011.01339.x. · DOI
  33. Proikas-Cezanne, 2004, WIPI-1alpha (WIPI49), a member of the novel 7-bladed WIPI protein family, is aberrantly expressed in human cancer and is linked to starvation-induced autophagy, Oncogene, 23, 9314, 10.1038/sj.onc.1208331 · DOI · Paper page
  34. Rubinsztein, 2009, In search of an “autophagomometer”, Autophagy, 5, 585, 10.4161/auto.5.5.8823 · DOI · Paper page
  35. Yang, 2010, GSK-3beta promotes cell survival by modulating Bif-1-dependent autophagy and cell death, J Cell Sci, 123, 861, 10.1242/jcs.060475 · DOI

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Metadata

Authors
Pfisterer, S. G., Mauthe, M., Codogno, P., & Proikas-Cezanne, T.
Venue
Molecular Pharmacology
Publisher
Elsevier BV
Published
2011-12
Volume
80
Issue
6
Pages
1066-1075
ISSN
0026-895X
DOI
10.1124/mol.111.071761
Cited by
78
References
6
Language
en
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