The DOI matches its Crossref record.
Role of Importin-β in Coupling Ran to Downstream Targets in Microtubule Assembly
Abstract
The guanosine triphosphatase Ran stimulates assembly of microtubule asters and spindles in mitotic Xenopus egg extracts. A carboxyl-terminal region of the nuclear-mitotic apparatus protein (NuMA), a nuclear protein required for organizing mitotic spindle poles, mimics Ran's ability to induce asters. This NuMA fragment also specifically interacted with the nuclear transport factor, importin-β. We show that importin-β is an inhibitor of microtubule aster assembly in Xenopus egg extracts and that Ran regulates the interaction between importin-β and NuMA. Importin-β therefore links NuMA to regulation by Ran. This suggests that similar mechanisms regulate nuclear import during interphase and spindle assembly during mitosis.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cited by
Whitfield, M. L., Sherlock, G., Saldanha, A. J., Murray, J. I., Ball, C. A., Alexander, K. E., Matese, J. C., Perou, C. M., Hurt, M. M., Brown, P. O., et al. (2002) · Molecular Biology of the Cell · doi:10.1091/mbc.02-02-0030 · Cited by 1,527
The RanGTP gradient—a GPS for the mitotic spindle
Kalab, P., & Heald, R. (2008) · Journal of Cell Science · doi:10.1242/jcs.005959 · Cited by 293
Silljé, H. H. W., Nagel, S., Körner, R., & Nigg, E. A. (2006) · Current Biology · doi:10.1016/j.cub.2006.02.070 · Cited by 267
Cai, S., Weaver, L. N., Ems-McClung, S. C., & Walczak, C. E. (2009) · Molecular Biology of the Cell · doi:10.1091/mbc.e08-09-0971 · Cited by 189
Albertson, R., Chabu, C., Sheehan, A., & Doe, C. Q. (2004) · Journal of Cell Science · doi:10.1242/jcs.01525 · Cited by 135
Kif18B interacts with EB1 and controls astral microtubule length during mitosis
Stout, J. R., Yount, A. L., Powers, J. A., LeBlanc, C., Ems-McClung, S. C., & Walczak, C. E. (2011) · Molecular Biology of the Cell · doi:10.1091/mbc.e11-04-0363 · Cited by 111
Aurora A phosphorylates MCAK to control ran-dependent spindle bipolarity
Zhang, X., Ems-McClung, S. C., & Walczak, C. E. (2008) · Molecular Biology of the Cell · doi:10.1091/mbc.e08-02-0198 · Cited by 123
A picornavirus protein interacts with Ran-GTPase and disrupts nucleocytoplasmic transport
Porter, F. W., Bochkov, Y. A., Albee, A. J., Wiese, C., & Palmenberg, A. C. (2006) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0605375103 · Cited by 112
Self-citationMicrotubule assembly during mitosis – from distinct origins to distinct functions?
Meunier, S., & Vernos, I. (2012) · Journal of Cell Science · doi:10.1242/jcs.092429 · Cited by 112
Spindle assembly in the absence of a RanGTP gradient requires localized CPC activity
Maresca, T. J., Groen, A. C., Gatlin, J. C., Ohi, R., Mitchison, T. J., & Salmon, E. D. (2009) · Current Biology · doi:10.1016/j.cub.2009.05.061 · Cited by 89
Tahara, K., Takagi, M., Ohsugi, M., Sone, T., Nishiumi, F., Maeshima, K., Horiuchi, Y., Tokai-Nishizumi, N., Imamoto, F., Yamamoto, T., et al. (2008) · The Journal of Cell Biology · doi:10.1083/jcb.200708003 · Cited by 53
Ran-dependent TPX2 activation promotes acentrosomal microtubule nucleation in neurons
Chen, W.-S., Chen, Y.-J., Huang, Y.-A., Hsieh, B.-Y., Chiu, H.-C., Kao, P.-Y., Chao, C.-Y., & Hwang, E. (2017) · Scientific Reports · doi:10.1038/srep42297 · Cited by 41
CENP-32 is required to maintain centrosomal dominance in bipolar spindle assembly
Ohta, S., Wood, L., Toramoto, I., Yagyu, K.-I., Fukagawa, T., & Earnshaw, W. C. (2015) · Molecular Biology of the Cell · doi:10.1091/mbc.e14-09-1366 · Cited by 16
Metadata
- Authors
- Wiese, C., Wilde, A., Moore, M. S., Adam, S. A., Merdes, A., & Zheng, Y.
- Venue
- Science
- Published
- 2001-01-26
- Volume
- 291
- Issue
- 5504
- Pages
- 653-656
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2036760739
- Cited by
- 323
- References
- 0
- FWCI
- 12.60
- Type
- journal article
- Language
- en
- Open access
- closed
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
