The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Enlarging the Amino Acid Set of Escherichia coli by Infiltration of the Valine Coding Pathway
Abstract
Aminoacyl transfer RNA (tRNA) synthetases establish the rules of the genetic code by catalyzing the aminoacylation of tRNAs. For some synthetases, accuracy depends critically on an editing function at a site distinct from the aminoacylation site. Mutants of Escherichia coli that incorrectly charge tRNA Val with cysteine were selected after random mutagenesis of the whole chromosome. All mutations obtained were located in the editing site of valyl-tRNA synthetase. More than 20% of the valine in cellular proteins from such an editing mutant organism could be replaced with the noncanonical aminobutyrate, sterically similar to cysteine. Thus, the editing function may have played a central role in restricting the genetic code to 20 amino acids. Disabling this editing function offers a powerful approach for diversifying the chemical composition of proteins and for emulating evolutionary stages of ambiguous translation.
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Cites
The frequency of errors in protein biosynthesis
Loftfield, R. B., & Vanderjagt, D. (1972) · Biochemical Journal · doi:10.1042/bj1281353 · Cited by 399
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Chin, J. W., Cropp, T. A., Anderson, J. C., Mukherji, M., Zhang, Z., & Schultz, P. G. (2003) · Science · doi:10.1126/science.1084772 · Cited by 820
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Xie, J., & Schultz, P. G. (2006) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm2005 · Cited by 408
Non-canonical amino acids in protein engineering
Link, A. J., Mock, M. L., & Tirrell, D. A. (2003) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2003.10.011 · Cited by 393
Driving change: the evolution of alternative genetic codes
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Metadata
- Authors
- Döring, V., Mootz, H. D., Nangle, L. A., Hendrickson, T. L., de Crécy-Lagard, V., Schimmel, P., & Marlière, P.
- Venue
- Science
- Published
- 2001-04-20
- Volume
- 292
- Issue
- 5516
- Pages
- 501-504
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2044865929
- Cited by
- 184
- References
- 1
- FWCI
- 7.71
- Type
- journal article
- Language
- en
- Open access
- closed
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- Döring, V., Mootz, H. D., Nangle, L. A., Hendrickson, T. L., de Crécy-Lagard, V., Schimmel, P., & Marlière, P. (2001). Enlarging the Amino Acid Set of Escherichia coli by Infiltration of the Valine Coding Pathway. Science. https://doi.org/10.1126/science.1057718
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
