Non-canonical amino acids in protein engineering

Expanding the Genetic Code of Escherichia coli

Wang, L., Brock, A., Herberich, B., & Schultz, P. G. (2001) · Science · doi:10.1126/science.1060077 · Cited by 1,504 · see citing works

A General Method for Site-specific Incorporation of Unnatural Amino Acids into Proteins

Noren, C. J., Anthony-Cahill, S. J., Griffith, M. C., & Schultz, P. G. (1989) · Science · doi:10.1126/science.2649980 · Cited by 1,019 · see citing works

Selenomethionyl proteins produced for analysis by multiwavelength anomalous diffraction (MAD): a vehicle for direct determination of three‐dimensional structure.

Hendrickson, W. A., Horton, J. R., & LeMaster, D. M. (1990) · The EMBO Journal · doi:10.1002/j.1460-2075.1990.tb08287.x · Cited by 1,015 · see citing works

Incorporation of azides into recombinant proteins for chemoselective modification by the Staudinger ligation

Kiick, K. L., Saxon, E., Tirrell, D. A., & Bertozzi, C. R. (2001) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.012583299 · Cited by 911 · see citing works

An Expanded Eukaryotic Genetic Code

Chin, J. W., Cropp, T. A., Anderson, J. C., Mukherji, M., Zhang, Z., & Schultz, P. G. (2003) · Science · doi:10.1126/science.1084772 · Cited by 820 · see citing works

Addition of a photocrosslinking amino acid to the genetic code of Escherichia coli

Chin, J. W., Martin, A., King, D., Wang, L., & Schultz, P. G. (2002) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.172226299 · Cited by 708 · see citing works

Cell Surface Labeling of Escherichia coli via Copper(I)-Catalyzed [3+2] Cycloaddition

Link, A. J., & Tirrell, D. A. (2003) · Journal of the American Chemical Society · doi:10.1021/ja036765z · Cited by 578 · see citing works

Addition of the keto functional group to the genetic code of Escherichia coli

Wang, L., Zhang, Z., Brock, A., & Schultz, P. G. (2002) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0234824100 · Cited by 356 · see citing works

Biosynthesis by Escherichia coli of active altered proteins containing selenium instead of sulfur

Cowie, D. B., & Cohen, G. N. (1957) · Biochimica et Biophysica Acta · doi:10.1016/0006-3002(57)90003-3 · Cited by 290 · see citing works

Generation of a Bacterium with a 21 Amino Acid Genetic Code

Mehl, R. A., Anderson, J. C., Santoro, S. W., Wang, L., Martin, A., King, D., Horn, D. M., & Schultz, P. G. (2003) · Journal of the American Chemical Society · doi:10.1021/ja0284153 · Cited by 287 · see citing works

Site-specific incorporation of an unnatural amino acid into proteins in mammalian cells

Sakamoto, K. (2002) · Nucleic Acids Research · doi:10.1093/nar/gkf589 · Cited by 283 · see citing works

Biosynthetic site-specific incorporation of a non-natural amino acid into a polypeptide

Bain, J. D., Diala, E. S., Glabe, C. G., Dix, T. A., & Chamberlin, A. R. (1989) · Journal of the American Chemical Society · doi:10.1021/ja00202a052 · Cited by 250 · see citing works

Unnatural amino acids as probes of protein structure and function

Dougherty, D. D. (2000) · Current Opinion in Chemical Biology · doi:10.1016/s1367-5931(00)00148-4 · Cited by 243 · see citing works

Nicotinic Receptor Binding Site Probed with Unnatural Amino Acid Incorporation in Intact Cells

Nowak, M. W., Kearney, P. C., Sampson, Saks, M. E., Labarca, C. G., Silverman, S. K., Zhong, W., Thorson, J. S., Abelson, J. N., Davidson, N., et al. (1995) · Science · doi:10.1126/science.7716551 · Cited by 230 · see citing works

Incorporation of non-natural amino acids into proteins

Hohsaka, T., & Sisido, M. (2002) · Current Opinion in Chemical Biology · doi:10.1016/s1367-5931(02)00376-9 · Cited by 218 · see citing works

Expansion of the genetic code: Site‐directed p‐fluoro‐phenylalanine incorporation in Escherichia coli

Furter, R. (1998) · Protein Science · doi:10.1002/pro.5560070223 · Cited by 216 · see citing works

An efficient system for the evolution of aminoacyl-tRNA synthetase specificity

Santoro, S. W., Wang, L., Herberich, B., King, D., & Schultz, P. G. (2002) · Nature Biotechnology · doi:10.1038/nbt742 · Cited by 211 · see citing works

Expansion of the Genetic Code Enables Design of a Novel “Gold” Class of Green Fluorescent Proteins

Bae, J. H., Rubini, M., Jung, G., Wiegand, G., Seifert, M. H. J., Azim, M. K., Kim, J.-S., Zumbusch, A., Holak, T. A., Moroder, L., et al. (2003) · Journal of Molecular Biology · doi:10.1016/s0022-2836(03)00364-4 · Cited by 204 · see citing works

A New Strategy for the Site-Specific Modification of Proteins in Vivo

Zhang, Z., Smith, B. A. C., Wang, L., Brock, A., Cho, C., & Schultz, P. G. (2003) · Biochemistry · doi:10.1021/bi0300231 · Cited by 191 · see citing works

Enlarging the Amino Acid Set of Escherichia coli by Infiltration of the Valine Coding Pathway

Döring, V., Mootz, H. D., Nangle, L. A., Hendrickson, T. L., de Crécy‐Lagard, V., SCHIMMEL, P. R., & Marlière, P. (2001) · Science · doi:10.1126/science.1057718 · Cited by 183 · see citing works

Biosynthesis of Proteins Incorporating a Versatile Set of Phenylalanine Analogues

Kirshenbaum, K., Carrico, I. S., & Tirrell, D. A. (2002) · ChemBioChem · doi:10.1002/1439-7633(20020301)3:2/3 · Cited by 172 · see citing works

Biosynthesis of a Highly Stable Coiled-Coil Protein Containing Hexafluoroleucine in an Engineered Bacterial Host

Tang, Y., & Tirrell, D. A. (2001) · Journal of the American Chemical Society · doi:10.1021/ja016652k · Cited by 170 · see citing works

Programmed Self-Sorting of Coiled Coils with Leucine and Hexafluoroleucine Cores

Bilgiçer, B., Xing, X., & Kumar, K. (2001) · Journal of the American Chemical Society · doi:10.1021/ja016767o · Cited by 169 · see citing works

A Coiled Coil with a Fluorous Core

Bilgiçer, B., Fichera, A., & Kumar, K. (2001) · Journal of the American Chemical Society · doi:10.1021/ja002961j · Cited by 164 · see citing works

Stabilization of Coiled-Coil Peptide Domains by Introduction of Trifluoroleucine

Tang, Y., Ghirlanda, G., Vaidehi, N., Kua, J., Mainz, D. T., Goddard, W. A., DeGrado, W. F., & Tirrell, D. A. (2001) · Biochemistry · doi:10.1021/bi0022588 · Cited by 164 · see citing works

Enhanced d -Amino Acid Incorporation into Protein by Modified Ribosomes

Dedkova, L. M., Fahmi, N. E., Golovine, S. Y., & Hecht, S. M. (2003) · Journal of the American Chemical Society · doi:10.1021/ja035141q · Cited by 157 · see citing works

In Vivo Photocrosslinking with Unnatural Amino Acid Mutagenesis

Chin, J. W., & Schultz, P. G. (2002) · ChemBioChem · doi:10.1002/1439-7633(20021104)3:11 · Cited by 149 · see citing works

A Designed Phenylalanyl-tRNA Synthetase Variant Allows Efficient in Vivo Incorporation of Aryl Ketone Functionality into Proteins

Datta, D., Wang, P., Carrico, I. S., Mayo, S. L., & Tirrell, D. A. (2002) · Journal of the American Chemical Society · doi:10.1021/ja0177096 · Cited by 139 · see citing works

Backbone Mutations in Transmembrane Domains of a Ligand-Gated Ion Channel

England, P. M., Zhang, Y., Dougherty, D. A., & Lester, H. A. (1999) · Cell · doi:10.1016/s0092-8674(00)80962-9 · Cited by 137 · see citing works

Expanding the genetic code: selection of efficient suppressors of four-base codons and identification of “shifty” four-base codons with a library approach in Escherichia coli11Edited by M. Gottesman

Magliery, T. J., Anderson, J. C., & Schultz, P. G. (2001) · Journal of Molecular Biology · doi:10.1006/jmbi.2001.4518 · Cited by 135 · see citing works

Breaking the Degeneracy of the Genetic Code

Kwon, I., Kirshenbaum, K., & Tirrell, D. A. (2003) · Journal of the American Chemical Society · doi:10.1021/ja0350076 · Cited by 132 · see citing works

Substrate Specificity Is Determined by Amino Acid Binding Pocket Size in Escherichia coli Phenylalanyl-tRNA Synthetase

Ibba, M., Kast, P., & Hennecke, H. (1994) · Biochemistry · doi:10.1021/bi00189a013 · Cited by 129 · see citing works

Incorporation of Trifluoroisoleucine into Proteins in Vivo

Wang, P., Tang, Y., & Tirrell, D. A. (2003) · Journal of the American Chemical Society · doi:10.1021/ja0298287 · Cited by 129 · see citing works

Adaptation of an Orthogonal Archaeal Leucyl-tRNA and Synthetase Pair for Four-base, Amber, and Opal Suppression

Anderson, J. C., & Schultz, P. G. (2003) · Biochemistry · doi:10.1021/bi034550w · Cited by 118 · see citing works

A Method for the Generation of Glycoprotein Mimetics

Liu, H., Wang, L., Brock, A., Wong, C., & Schultz, P. G. (2003) · Journal of the American Chemical Society · doi:10.1021/ja029433n · Cited by 118 · see citing works

Unnatural Amino Acid Mutagenesis of Green Fluorescent Protein

Wang, L., Xie, J., Deniz, A. A., & Schultz, P. G. (2002) · The Journal of Organic Chemistry · doi:10.1021/jo026570u · Cited by 109 · see citing works

Structure of Homoleptic CuI(CO)3 Cations in CuI-Exchanged ZSM-5 Zeolite: An X-ray Absorption Study

Lamberti, C., Palomino, G. T., Bordiga, S., Berlier, G., d’Acapito, F., & Zecchina, A. (2000) · Angewandte Chemie International Edition · doi:10.1002/1521-3773(20000616)39:12 · Cited by 103 · see citing works

Attenuation of the Editing Activity of theEscherichia coliLeucyl-tRNA Synthetase Allows Incorporation of Novel Amino Acids into Proteins in Vivo

Tang, Y., & Tirrell, D. A. (2002) · Biochemistry · doi:10.1021/bi026130x · Cited by 99 · see citing works

Efficient introduction of aryl bromide functionality into proteins in vivo

Sharma, N., Furter, R., Kast, P., & Tirrell, D. A. (2000) · FEBS Letters · doi:10.1016/s0014-5793(00)01120-0 · Cited by 99 · see citing works

Slow Exchange in the Chromophore of a Green Fluorescent Protein Variant

Seifert, M. H. J., Ksiazek, D., Azim, M. K., Smialowski, P., Budiša, N., & Holak, T. A. (2002) · Journal of the American Chemical Society · doi:10.1021/ja0257725 · Cited by 95 · see citing works

Exploring the Limits of Codon and Anticodon Size

Anderson, J. C., Magliery, T. J., & Schultz, P. G. (2002) · Chemistry & Biology · doi:10.1016/s1074-5521(02)00094-7 · Cited by 88 · see citing works

Five-base codons for incorporation of nonnatural amino acids into proteins

Hohsaka, T. (2001) · Nucleic Acids Research · doi:10.1093/nar/29.17.3646 · Cited by 85 · see citing works

Global Replacement of Tryptophan with Aminotryptophans Generates Non-Invasive Protein-Based Optical pH Sensors

Budiša, N., Rubini, M., Bae, J. H., Weyher, E., Wenger, W., Golbik, R. P., Huber, R., & Moroder, L. (2002) · Angewandte Chemie International Edition · doi:10.1002/1521-3773(20021104)41:21 · Cited by 82 · see citing works

In Vitro Selection of mRNA Display Libraries Containing an Unnatural Amino Acid

Li, S., Millward, S. W., & Roberts, R. W. (2002) · Journal of the American Chemical Society · doi:10.1021/ja026789q · Cited by 79 · see citing works

Synthesis and thermodynamic characterization of self-sorting coiled coils

Bilgiçer, B., & Kumar, K. (2002) · Tetrahedron · doi:10.1016/s0040-4020(02)00260-0 · Cited by 74 · see citing works

Relaxing the substrate specificity of an aminoacyl‐tRNA synthetase allows in vitro and in vivo synthesis of proteins containing unnatural amino acids

Ibba, M., & Hennecke, H. (1995) · FEBS Letters · doi:10.1016/0014-5793(95)00408-2 · Cited by 72 · see citing works

Fluorescence Resonance Energy Transfer between Unnatural Amino Acids in a Structurally Modified Dihydrofolate Reductase

Anderson, R. D., Zhou, J., & Hecht, S. M. (2002) · Journal of the American Chemical Society · doi:10.1021/ja0205939 · Cited by 65 · see citing works

Site-Specific Incorporation of Unnatural Amino Acids into Receptors Expressed in Mammalian Cells

Monahan, S. L., Lester, H. A., & Dougherty, D. A. (2003) · Chemistry & Biology · doi:10.1016/s1074-5521(03)00124-8 · Cited by 61 · see citing works

Position-Specific Incorporation of a Fluorophore−Quencher Pair into a Single Streptavidin through Orthogonal Four-Base Codon/Anticodon Pairs

Taki, M., Hohsaka, T., Murakami, H., Taira, K., & Sisido, M. (2002) · Journal of the American Chemical Society · doi:10.1021/ja017714+ · Cited by 60 · see citing works

Real-time and equilibrium 19 F-NMR studies reveal the role of domain–domain interactions in the folding of the chaperone PapD

Bann, J. G., Pinkner, J. S., Hultgren, S. J., & Frieden, C. (2002) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.022649599 · Cited by 59 · see citing works

Incorporation of β-selenolo[3,2-b]pyrrolyl-alanine into proteins for phase determination in protein X-ray crystallography

Bae, J. H., Alefelder, S., Kaiser, J. T., Friedrich, R., Moroder, L., Huber, R., & Budiša, N. (2001) · Journal of Molecular Biology · doi:10.1006/jmbi.2001.4699 · Cited by 46 · see citing works

Site-Specific Incorporation of (Aminooxy)acetic Acid into Proteins

Eisenhauer, B. M., & Hecht, S. M. (2002) · Biochemistry · doi:10.1021/bi020352d · Cited by 33 · see citing works

A Perturbed pKa at the Binding Site of the Nicotinic Acetylcholine Receptor: Implications for Nicotine Binding

Petersson, E. J., Choi, A., Dahan, D. S., Lester, H. A., & Dougherty, D. A. (2002) · Journal of the American Chemical Society · doi:10.1021/ja028206i · Cited by 32 · see citing works

In vitro selection for sense codon suppression

Frankel, A., & Roberts, R. W. (2003) · RNA · doi:10.1261/rna.5350303 · Cited by 30 · see citing works

Chemically Mediated Site-Specific Proteolysis. Alteration of Protein−Protein Interaction

Wang, B., Brown, K. C., Lodder, M., Craik, C. S., & Hecht, S. M. (2002) · Biochemistry · doi:10.1021/bi011762p · Cited by 24 · see citing works

Structure-based design of mutant Methanococcus jannaschii tyrosyl-tRNA synthetase for incorporation of O -methyl- l -tyrosine

Zhang, D., Vaidehi, N., Goddard, W. A., Danzer, J. F., & Debe, D. A. (2002) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.052150499 · Cited by 24 · see citing works

Der Tetracyclin-Repressor – das Musterbeispiel für einen biologischen Schalter

Saenger, W., Orth, P., Kisker, C., Hillen, W., & Hinrichs, W. (2000) · Angewandte Chemie · doi:10.1002/1521-3757(20000616)112:12 · Cited by 9 · see citing works

Bioorthogonal Chemistry: Fishing for Selectivity in a Sea of Functionality

Sletten, E. M., & Bertozzi, C. R. (2009) · Angewandte Chemie International Edition · doi:10.1002/anie.200900942 · Cited by 3,165 · see citing works

Adding New Chemistries to the Genetic Code

Liu, C. C., & Schultz, P. G. (2010) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.052308.105824 · Cited by 1,725 · see citing works

Chemistry in living systems

Prescher, J. A., & Bertozzi, C. R. (2005) · Nature Chemical Biology · doi:10.1038/nchembio0605-13 · Cited by 1,464 · see citing works

Hydrogel biomaterials: A smart future?

Kopec̆ek, J. (2007) · Biomaterials · doi:10.1016/j.biomaterials.2007.07.044 · Cited by 1,004 · see citing works

Tetrazine-Based Cycloadditions: Application to Pretargeted Live Cell Imaging

Devaraj, N. K., Weissleder, R., & Hilderbrand, S. A. (2008) · Bioconjugate Chemistry · doi:10.1021/bc8004446 · Cited by 859 · see citing works

Biocatalysis in Organic Chemistry and Biotechnology: Past, Present, and Future

Reetz, M. T. (2013) · Journal of the American Chemical Society · doi:10.1021/ja405051f · Cited by 762 · see citing works

Chemoselective Ligation and Modification Strategies for Peptides and Proteins

Hackenberger, C. P. R., & Schwarzer, D. (2008) · Angewandte Chemie International Edition · doi:10.1002/anie.200801313 · Cited by 760 · see citing works

A Comparative Study of Bioorthogonal Reactions with Azides

Agard, N. J., Baskin, J. M., Prescher, J. A., Lo, A., & Bertozzi, C. R. (2006) · ACS Chemical Biology · doi:10.1021/cb6003228 · Cited by 744 · see citing works

Gelatinase-mediated migration and invasion of cancer cells

Björklund, M., & Koivunen, E. (2005) · Biochimica et Biophysica Acta (BBA) - Reviews on Cancer · doi:10.1016/j.bbcan.2005.03.001 · Cited by 663 · see citing works

Chemical Modification of Proteins at Cysteine: Opportunities in Chemistry and Biology

Chalker, J. M., Bernardes, G. J. L., Lin, Y. A., & Davis, B. G. (2009) · Chemistry - An Asian Journal · doi:10.1002/asia.200800427 · Cited by 653 · see citing works

Click Chemistry and Bioorthogonal Reactions: Unprecedented Selectivity in the Labeling of Biological Molecules

Best, M. D. (2009) · Biochemistry · doi:10.1021/bi9007726 · Cited by 647 · see citing works

Choosing an effective protein bioconjugation strategy

Stephanopoulos, N., & Francis, M. B. (2011) · Nature Chemical Biology · doi:10.1038/nchembio.720 · Cited by 636 · see citing works

Bioorthogonale Chemie – oder: in einem Meer aus Funktionalität nach Selektivität fischen

Sletten, E. M., & Bertozzi, C. R. (2009) · Angewandte Chemie · doi:10.1002/ange.200900942 · Cited by 564 · see citing works

Modern trends in polymer bioconjugates design

Lutz, J., & Börner, H. G. (2007) · Progress in Polymer Science · doi:10.1016/j.progpolymsci.2007.07.005 · Cited by 544 · see citing works

Peptide/protein–polymer conjugates: synthetic strategies and design concepts

Gauthier, M. A., & Klok, H. (2008) · Chemical Communications · doi:10.1039/b719689j · Cited by 499 · see citing works

Selective Covalent Protein Immobilization: Strategies and Applications

Wong, L. S., Khan, F., & Micklefield, J. (2009) · Chemical Reviews · doi:10.1021/cr8004668 · Cited by 489 · see citing works

Photo-leucine and photo-methionine allow identification of protein-protein interactions in living cells

Suchanek, M., Radzikowska, A., & Thiele, C. (2005) · Nature Methods · doi:10.1038/nmeth752 · Cited by 487 · see citing works

In situ visualization and dynamics of newly synthesized proteins in rat hippocampal neurons

Dieterich, D. C., Hodas, J. J. L., Gouzer, G., Shadrin, I. Y., Ngo, J. T., Triller, A., Tirrell, D. A., & Schuman, E. M. (2010) · Nature Neuroscience · doi:10.1038/nn.2580 · Cited by 470 · see citing works

Introducing genetically encoded aldehydes into proteins

Carrico, I. S., Carlson, B. L., & Bertozzi, C. R. (2007) · Nature Chemical Biology · doi:10.1038/nchembio878 · Cited by 438 · see citing works

Hydrogels: From soft contact lenses and implants to self‐assembled nanomaterials

Kopec̆ek, J. (2009) · Journal of Polymer Science Part A Polymer Chemistry · doi:10.1002/pola.23607 · Cited by 430 · see citing works

Hydrogels as smart biomaterials

Kopec̆ek, J., & Yang, J. (2007) · Polymer International · doi:10.1002/pi.2253 · Cited by 425 · see citing works

Towards organoid culture without Matrigel

Kozlowski, M. T., Crook, C. J., & Ku, H. T. (2021) · Communications Biology · doi:10.1038/s42003-021-02910-8 · Cited by 409 · see citing works

Selective in vivo metabolic cell-labeling-mediated cancer targeting

Wang, H., Wang, R., Cai, K., He, H., Liu, Y., Yen, J. S., Wang, Z., Xu, M., Sun, Y., Zhou, X., et al. (2017) · Nature Chemical Biology · doi:10.1038/nchembio.2297 · Cited by 387 · see citing works

Functionalized Cyclopropenes As Bioorthogonal Chemical Reporters

Patterson, D. M., Nazarova, L. A., Xie, B. J., Kamber, D. N., & Prescher, J. A. (2012) · Journal of the American Chemical Society · doi:10.1021/ja3060436 · Cited by 371 · see citing works

Molecular Nanoparticles Are Unique Elements for Macromolecular Science: From “Nanoatoms” to Giant Molecules

Zhang, W., Yu, X., Wang, C., Sun, H., Hsieh, I., Li, Y., Dong, X., Yue, K., Horn, R. M. V., & Cheng, S. Z. D. (2014) · Macromolecules · doi:10.1021/ma401724p · Cited by 354 · see citing works

Bioorthogonal Click Chemistry: Covalent Labeling in Living Systems

Baskin, J. M., & Bertozzi, C. R. (2007) · QSAR & Combinatorial Science · doi:10.1002/qsar.200740086 · Cited by 332 · see citing works

Protein Organic Chemistry and Applications for Labeling and Engineering in Live‐Cell Systems

Takaoka, Y., Ojida, A., & Hamachi, I. (2013) · Angewandte Chemie International Edition · doi:10.1002/anie.201207089 · Cited by 314 · see citing works

RNA Display Methods for the Discovery of Bioactive Macrocycles

Huang, Y., Wiedmann, M. M., & Suga, H. (2018) · Chemical Reviews · doi:10.1021/acs.chemrev.8b00430 · Cited by 309 · see citing works

Fluorescence Visualization of Newly Synthesized Proteins in Mammalian Cells

Beatty, K. E., Liu, J. C., Xie, F., Dieterich, D. C., Schuman, E. M., Wang, Q., & Tirrell, D. A. (2006) · Angewandte Chemie International Edition · doi:10.1002/anie.200602114 · Cited by 305 · see citing works

Robust Fluorescent Detection of Protein Fatty-Acylation with Chemical Reporters

Charron, G., Zhang, M. M., Yount, J. S., Wilson, J. P., Raghavan, A. S., Shamir, E. R., & Hang, H. C. (2009) · Journal of the American Chemical Society · doi:10.1021/ja810122f · Cited by 301 · see citing works

Evolution of translation machinery in recoded bacteria enables multi-site incorporation of nonstandard amino acids

Amiram, M., Haimovich, A. D., Fan, C., Wang, Y., Aerni, H. R., Ntai, I., Moonan, D. W., Ma, N. J., Rovner, A. J., Hong, S. H., et al. (2015) · Nature Biotechnology · doi:10.1038/nbt.3372 · Cited by 283 · see citing works

Reprogramming the Translation Initiation for the Synthesis of Physiologically Stable Cyclic Peptides

Goto, Y., Ohta, A., Sako, Y., Yamagishi, Y., Murakami, H., & Suga, H. (2008) · ACS Chemical Biology · doi:10.1021/cb700233t · Cited by 276 · see citing works

Prolegomena to Future Experimental Efforts on Genetic Code Engineering by Expanding Its Amino Acid Repertoire

Budiša, N. (2004) · Angewandte Chemie International Edition · doi:10.1002/anie.200300646 · Cited by 263 · see citing works

Bioorthogonal Click Chemistry: An Indispensable Tool to Create Multifaceted Cell Culture Scaffolds

Azagarsamy, M. A., & Anseth, K. S. (2012) · ACS Macro Letters · doi:10.1021/mz300585q · Cited by 253 · see citing works

Biocatalysis with Unnatural Amino Acids: Enzymology Meets Xenobiology

Agostini, F., Völler, J., Koksch, B., Acevedo‐Rocha, C. G., Kubyshkin, V., & Budiša, N. (2017) · Angewandte Chemie International Edition · doi:10.1002/anie.201610129 · Cited by 232 · see citing works

Ribosomal Synthesis of Unnatural Peptides

Josephson, K., Hartman, M. C. T., & Szostak, J. W. (2005) · Journal of the American Chemical Society · doi:10.1021/ja0515809 · Cited by 234 · see citing works

Transition metal catalyzed methods for site-selective protein modification

Antos, J. M., & Francis, M. B. (2006) · Current Opinion in Chemical Biology · doi:10.1016/j.cbpa.2006.04.009 · Cited by 226 · see citing works

Non-genetic engineering of cells for drug delivery and cell-based therapy

Wang, Q., Cheng, H., Peng, H., Zhou, H., Li, P. Y., & Langer, R. (2015) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2014.12.003 · Cited by 225 · see citing works

Expanding the Genetic Code for Biological Studies

Wang, Q., Parrish, A. R., & Wang, L. (2009) · Chemistry & Biology · doi:10.1016/j.chembiol.2009.03.001 · Cited by 224 · see citing works

Peptide-directed self-assembly of hydrogels

Kopec̆ek, J., & Yang, J. (2008) · Acta Biomaterialia · doi:10.1016/j.actbio.2008.10.001 · Cited by 222 · see citing works

Bioinspired functional block copolymers

Börner, H. G., & Schlaad, H. (2007) · Soft Matter · doi:10.1039/b615985k · Cited by 220 · see citing works

Chemoselektive Ligations‐ und Modifikationsstrategien für Peptide und Proteine

Hackenberger, C. P. R., & Schwarzer, D. (2008) · Angewandte Chemie · doi:10.1002/ange.200801313 · Cited by 220 · see citing works

Multifunctional Materials through Modular Protein Engineering

DiMarco, R. L., & Heilshorn, S. C. (2012) · Advanced Materials · doi:10.1002/adma.201200051 · Cited by 208 · see citing works

Using 19F NMR to Probe Biological Interactions of Proteins and Peptides

Marsh, E. N. G., & Suzuki, Y. (2014) · ACS Chemical Biology · doi:10.1021/cb500111u · Cited by 204 · see citing works

Strategies exploiting functions and self-assembly properties of bioconjugates for polymer and materials sciences

Börner, H. G. (2009) · Progress in Polymer Science · doi:10.1016/j.progpolymsci.2009.05.001 · Cited by 195 · see citing works

Growth factor delivery: How surface interactions modulate release in vitro and in vivo

King, W. J., & Krebsbach, P. H. (2012) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2012.03.004 · Cited by 192 · see citing works

Fluorinated Proteins: From Design and Synthesis to Structure and Stability

Marsh, E. N. G. (2014) · Accounts of Chemical Research · doi:10.1021/ar500125m · Cited by 190 · see citing works

Discovery of aminoacyl-tRNA synthetase activity through cell-surface display of noncanonical amino acids

Link, A. J., Vink, M. K. S., Agard, N. J., Prescher, J. A., Bertozzi, C. R., & Tirrell, D. A. (2006) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0601167103 · Cited by 189 · see citing works

Messenger RNA-Programmed Incorporation of Multiple N-Methyl-Amino Acids into Linear and Cyclic Peptides

Kawakami, T., Murakami, H., & Suga, H. (2008) · Chemistry & Biology · doi:10.1016/j.chembiol.2007.12.008 · Cited by 186 · see citing works

Proline Editing: A General and Practical Approach to the Synthesis of Functionally and Structurally Diverse Peptides. Analysis of Steric versus Stereoelectronic Effects of 4-Substituted Prolines on Conformation within Peptides

Pandey, A. K., Naduthambi, D., Thomas, K. M., & Zondlo, N. J. (2013) · Journal of the American Chemical Society · doi:10.1021/ja3109664 · Cited by 182 · see citing works

Bioorthogonal Chemistry for Site-Specific Labeling and Surface Immobilization of Proteins

Chen, Y., Triola, G., & Waldmann, H. (2011) · Accounts of Chemical Research · doi:10.1021/ar200046h · Cited by 181 · see citing works

Unnatural Amino Acid Incorporation into Virus-Like Particles

Strable, E., Prasuhn, D. E., Udit, A. K., Brown, S. D., Link, A. J., Ngo, J. T., Lander, G. C., Quispe, J. D., Potter, C. S., Carragher, B. O., et al. (2008) · Bioconjugate Chemistry · doi:10.1021/bc700390r · Cited by 181 · see citing works

Polymer–protein conjugates: an enzymatic activity perspective

Gauthier, M. A., & Klok, H. (2010) · Polymer Chemistry · doi:10.1039/c0py90001j · Cited by 169 · see citing works

Recent advances in developing active targeting and multi-functional drug delivery systems via bioorthogonal chemistry

Yi, W., Xiao, P., Liu, X., Zhao, Z., Sun, X., Wang, J., Zhou, L., Wang, G., Cao, H., Wang, D., et al. (2022) · Signal Transduction and Targeted Therapy · doi:10.1038/s41392-022-01250-1 · Cited by 173 · see citing works

A Genetically Encoded Bidentate, Metal‐Binding Amino Acid

Xie, J., Liu, W. R., & Schultz, P. G. (2007) · Angewandte Chemie International Edition · doi:10.1002/anie.200703397 · Cited by 166 · see citing works

Computational design of peptide ligands

Vanhee, P., van der Sloot, A. M., Verschueren, E., Serrano, L. G., Rousseau, F., & Schymkowitz, J. W. H. (2011) · Trends in biotechnology · doi:10.1016/j.tibtech.2011.01.004 · Cited by 167 · see citing works

Synthetic Biology: Advancing the Design of Diverse Genetic Systems

Wang, Y., Wei, K. Y., & Smolke, C. D. (2013) · Annual Review of Chemical and Biomolecular Engineering · doi:10.1146/annurev-chembioeng-061312-103351 · Cited by 157 · see citing works

Protein-Engineered Biomaterials: Highly Tunable Tissue Engineering Scaffolds

Sengupta, D., & Heilshorn, S. C. (2010) · Tissue Engineering Part B Reviews · doi:10.1089/ten.teb.2009.0591 · Cited by 157 · see citing works

Olefin Metathesis for Site‐Selective Protein Modification

Lin, Y. A., Chalker, J. M., & Davis, B. G. (2009) · ChemBioChem · doi:10.1002/cbic.200900002 · Cited by 154 · see citing works

Polypeptoids: a model system to study the effect of monomer sequence on polymer properties and self-assembly

Rosales, A. M., Segalman, R. A., & Zuckermann, R. N. (2013) · Soft Matter · doi:10.1039/c3sm51421h · Cited by 147 · see citing works

Fluorine in a Native Protein Environment—How the Spatial Demand and Polarity of Fluoroalkyl Groups Affect Protein Folding

Jäckel, C., Salwiczek, M., & Koksch, B. (2006) · Angewandte Chemie International Edition · doi:10.1002/anie.200504387 · Cited by 146 · see citing works

Genetically Encoded Azide Containing Amino Acid in Mammalian Cells Enables Site-Specific Antibody–Drug Conjugates Using Click Cycloaddition Chemistry

VanBrunt, M. P., Shanebeck, K. D., Caldwell, Z., Johnson, J. E., Thompson, P., Martin, T., Dong, H., Li, G., Xu, H., D’Hooge, F., et al. (2015) · Bioconjugate Chemistry · doi:10.1021/acs.bioconjchem.5b00359 · Cited by 145 · see citing works

Chemoselective Staudinger‐Phosphite Reaction of Azides for the Phosphorylation of Proteins

Serwa, R. A., Wilkening, I., Signore, G. D., Mühlberg, M., Claußnitzer, I., Weise, C., Gerrits, M., & Hackenberger, C. P. R. (2009) · Angewandte Chemie International Edition · doi:10.1002/anie.200902118 · Cited by 145 · see citing works

Efficient N‐Terminal Labeling of Proteins by Use of Sortase

Williamson, D. J., Fascione, M. A., Webb, M. E., & Turnbull, W. B. (2012) · Angewandte Chemie International Edition · doi:10.1002/anie.201204538 · Cited by 138 · see citing works

A Genetically Encoded Bidentate, Metal‐Binding Amino Acid

Xie, J., Liu, W. R., & Schultz, P. G. (2007) · Angewandte Chemie · doi:10.1002/ange.200703397 · Cited by 131 · see citing works

Design and engineering of metalloproteins containing unnatural amino acids or non-native metal-containing cofactors

Lu, Y. (2005) · Current Opinion in Chemical Biology · doi:10.1016/j.cbpa.2005.02.017 · Cited by 130 · see citing works

Introducing Bioorthogonal Functionalities into Proteins in Living Cells

Hao, Z., Hong, S., Chen, X., & Chen, P. R. (2011) · Accounts of Chemical Research · doi:10.1021/ar200067r · Cited by 129 · see citing works

Universal Gradient Substrates for “Click” Biofunctionalization

Gallant, N. D., Lavery, K. A., Amis, E. J., & Becker, M. L. (2007) · Advanced Materials · doi:10.1002/adma.200602221 · Cited by 126 · see citing works

Materiomics: An ‐omics Approach to Biomaterials Research

Cranford, S. W., de Boer, J., van Blitterswijk, C., & Buehler, M. J. (2013) · Advanced Materials · doi:10.1002/adma.201202553 · Cited by 125 · see citing works

Protein Mechanics: From Single Molecules to Functional Biomaterials

Li, H., & Cao, Y. (2010) · Accounts of Chemical Research · doi:10.1021/ar100057a · Cited by 124 · see citing works

Reprogramming Nonribosomal Peptide Synthetases for “Clickable” Amino Acids

Kries, H., Wachtel, R., Pabst, A., Wanner, B. M., Niquille, D. L., & Hilvert, D. (2014) · Angewandte Chemie International Edition · doi:10.1002/anie.201405281 · Cited by 123 · see citing works

Chemical modification in the creation of novel biocatalysts

Díaz‐Rodríguez, A., & Davis, B. G. (2011) · Current Opinion in Chemical Biology · doi:10.1016/j.cbpa.2010.12.002 · Cited by 114 · see citing works

Bioengineering Strategies for Protein-Based Nanoparticles

Diaz, D., Care, A., & Sunna, A. (2018) · Genes · doi:10.3390/genes9070370 · Cited by 110 · see citing works

Reassignment of sense codons in vivo

Link, A. J., & Tirrell, D. A. (2005) · Methods · doi:10.1016/j.ymeth.2005.04.005 · Cited by 110 · see citing works

Molecular tools for the construction of peptide-based materials

Ramakers, B. E. I., van Hest, J. C. M., & Löwik, D. W. P. M. (2014) · Chemical Society Reviews · doi:10.1039/c3cs60362h · Cited by 108 · see citing works

A fluorogenic ‘click’ reaction of azidoanthracene derivatives

Xie, F., Sivakumar, K., Zeng, Q., Bruckman, M. A., Hodges, B., & Wang, Q. (2008) · Tetrahedron · doi:10.1016/j.tet.2008.01.080 · Cited by 108 · see citing works

Recent advances in genetic code engineering in Escherichia coli

Hoesl, M. G., & Budiša, N. (2012) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2011.12.027 · Cited by 107 · see citing works

19F NMR as a tool in chemical biology

Giménez, D., Phelan, A., Murphy, C. D., & Cobb, S. L. (2021) · Beilstein Journal of Organic Chemistry · doi:10.3762/bjoc.17.28 · Cited by 106 · see citing works

Plasma Clearance of Bacteriophage Qβ Particles as a Function of Surface Charge

Prasuhn, D. E., Singh, P. N. P., Strable, E., Brown, S. D., Manchester, M., & Finn, M. G. (2008) · Journal of the American Chemical Society · doi:10.1021/ja075937f · Cited by 104 · see citing works

Evolution of a fluorinated green fluorescent protein

Yoo, T. H., Link, A. J., & Tirrell, D. A. (2007) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0701904104 · Cited by 103 · see citing works

Regioselective Carbon–Carbon Bond Formation in Proteins with Palladium Catalysis; New Protein Chemistry by Organometallic Chemistry

Kodama, K., Fukuzawa, S., Nakayama, H., Kigawa, T., Sakamoto, K., Yabuki, T., Matsuda, N., Shirouzu, M., Takio, K., Tachibana, K., et al. (2005) · ChemBioChem · doi:10.1002/cbic.200500290 · Cited by 102 · see citing works

Site‐Selective Disulfide Modification of Proteins: Expanding Diversity beyond the Proteome

Kuan, S. L., Wang, T., & Weil, T. (2016) · Chemistry - A European Journal · doi:10.1002/chem.201602298 · Cited by 102 · see citing works

Protein‐Engineered Functional Materials

Wang, Y., Katyal, P., & Montclare, J. K. (2019) · Advanced Healthcare Materials · doi:10.1002/adhm.201801374 · Cited by 102 · see citing works

Motif mediated protein-protein interactions as drug targets

Corbi‐Verge, C., & Kim, P. M. (2016) · Cell Communication and Signaling · doi:10.1186/s12964-016-0131-4 · Cited by 103 · see citing works

Targeted metabolic labeling of yeast N-glycans with unnatural sugars

Breidenbach, M. A., Gallagher, J. E. G., King, D., Smart, B. P., Wu, P., & Bertozzi, C. R. (2010) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0911247107 · Cited by 100 · see citing works

Structural basis for the enhanced stability of highly fluorinated proteins

Buer, B. C., Meagher, J. L., Stuckey, J. A., & Marsh, E. N. G. (2012) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1120112109 · Cited by 99 · see citing works

Cell-selective labelling of proteomes in Drosophila melanogaster

Erdmann, I., Marter, K., Kobler, O., Niehues, S., Abele, J., Müller, A., Bussmann, J., Storkebaum, E., Ziv, T., Thomas, U., et al. (2015) · Nature Communications · doi:10.1038/ncomms8521 · Cited by 98 · see citing works

Cell-Membrane-Based Biomimetic Systems with Bioorthogonal Functionalities

Huang, L., Nie, W., Zhang, J., & Xie, H. (2020) · Accounts of Chemical Research · doi:10.1021/acs.accounts.9b00559 · Cited by 98 · see citing works

Translation Initiation with Initiator tRNA Charged with Exotic Peptides

Goto, Y., & Suga, H. (2009) · Journal of the American Chemical Society · doi:10.1021/ja900597d · Cited by 96 · see citing works

Surface re-engineering of pancreatic islets with recombinant azido-thrombomodulin.

Stabler, C., Sun, X.-L., Cui, W., Wilson, J. T., Haller, C., & Chaikof, E. (2007) · Bioconjugate chemistry · doi:10.1021/bc7002814 · Cited by 96 · see citing works

Synthesis and Application of Protein-Containing Block Copolymers

Obermeyer, A. C., & Olsen, B. D. (2015) · ACS Macro Letters · doi:10.1021/mz500732e · Cited by 94 · see citing works

Status of Biocatalysis in the Production of 2,5-Furandicarboxylic Acid

Troiano, D. T., Orsat, V., & Dumont, M. (2020) · ACS Catalysis · doi:10.1021/acscatal.0c02378 · Cited by 94 · see citing works

Highly Efficient Coupling of β-Substituted Aminoethane Sulfonyl Azides with Thio Acids, toward a New Chemical Ligation Reaction

Merkx, R., Brouwer, A. J., Rijkers, D. T. S., & Liskamp, R. M. J. (2005) · Organic Letters · doi:10.1021/ol0501119 · Cited by 92 · see citing works

Synthesis at the Interface of Chemistry and Biology

Wu, X., & Schultz, P. G. (2009) · Journal of the American Chemical Society · doi:10.1021/ja9026067 · Cited by 90 · see citing works

Small peptides, big world: biotechnological potential in neglected bioactive peptides from arthropod venoms

Pimenta, A. M. C., & de Lima, M. E. (2005) · Journal of Peptide Science · doi:10.1002/psc.701 · Cited by 89 · see citing works

The allylic chalcogen effect in olefin metathesis

Lin, Y. A., & Davis, B. G. (2010) · Beilstein Journal of Organic Chemistry · doi:10.3762/bjoc.6.140 · Cited by 88 · see citing works

Principles of Gene Manipulation and Genomics

Primrose, S. B., & Twyman, R. M. (2002) · Cited by 88 · see citing works

Non-canonical amino acid labeling in proteomics and biotechnology

Saleh, A. M., Wilding, K. M., Calve, S., Bundy, B. C., & Kinzer‐Ursem, T. L. (2019) · Journal of Biological Engineering · doi:10.1186/s13036-019-0166-3 · Cited by 87 · see citing works

A natural genetic code expansion cassette enables transmissible biosynthesis and genetic encoding of pyrrolysine

Longstaff, D. G., Larue, R. C., Faust, J. E., Mahapatra, A., Zhang, L., Green‐Church, K. B., & Krzycki, J. A. (2007) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0610294104 · Cited by 87 · see citing works

A review of combined experimental and computational procedures for assessing biopolymer structure–process–property relationships

Gronau, G., Krishnaji, S. T., Kinahan, M. E., Giesa, T., Wong, J. Y., Kaplan, D. L., & Buehler, M. J. (2012) · Biomaterials · doi:10.1016/j.biomaterials.2012.06.054 · Cited by 88 · see citing works

Protein Engineering mit nicht-kanonischen Aminosäuren

Hotz, N., Marzluf, T., & Wiltschi, B. (2012) · BIOspektrum · doi:10.1007/s12268-012-0146-3

Residue-specific incorporation of non-canonical amino acids into proteins: recent developments and applications

Johnson, J. A., Lu, Y. Y., Deventer, J. A. V., & Tirrell, D. A. (2010) · Current Opinion in Chemical Biology · doi:10.1016/j.cbpa.2010.09.013 · Cited by 320 · see citing works

From Multi-fluorinated DNA Polymerases to Insights into DNA Synthesis by NMR spectroscopy

Holzberger, B. (2012) · KOPS (University of Konstanz)

Residue-specific Incorporation of Noncanonical Amino Acids into Model Proteins Using an Escherichia coli Cell-free Transcription-translation System

Worst, E. G., Exner, M., Simone, A. D., Schenkelberger, M., Noireaux, V., Budiša, N., & Ott, A. (2016) · Journal of Visualized Experiments · doi:10.3791/54273 · Cited by 15 · see citing works

Incorporation of Non-Canonical Amino Acids into Proteins by Global Reassignment of Sense Codons

Fang, K. Y., Lieblich, S. A., & Tirrell, D. A. (2018) · Methods in molecular biology · doi:10.1007/978-1-4939-7893-9_13 · Cited by 14 · see citing works