Cited by 760Microbial Natural Products and BiosynthesisLipid metabolism and biosynthesisMicrobial Metabolic Engineering and Bioproduction

Production of polyunsaturated fatty acids by polyketide synthases in both prokaryotes and eukaryotes.

Metz, James G., Roessler, Paul, Facciotti, Daniel, Levering, Charlene, Dittrich, Franziska, Lassner, Michael, Valentine, Ray, Lardizabal, Kathryn +5 more·2001·Science

Abstract

Polyunsaturated fatty acids (PUFAs) are essential membrane components in higher eukaryotes and are the precursors of many lipid-derived signaling molecules. Here, pathways for PUFA synthesis are described that do not require desaturation and elongation of saturated fatty acids. These pathways are catalyzed by polyketide synthases (PKSs) that are distinct from previously recognized PKSs in both structure and mechanism. Generation of cis double bonds probably involves position-specific isomerases; such enzymes might be useful in the production of new families of antibiotics. It is likely that PUFA synthesis in cold marine ecosystems is accomplished in part by these PKS enzymes.

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Crossref · 10.1126/science.1059593

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Authors
Metz, J. G., Roessler, P., Facciotti, D., Levering, C., Dittrich, F., Lassner, M., Valentine, R., Lardizabal, K., Domergue, F., Yamada, A., Yazawa, K., Knauf, V., & Browse, J.
Affiliations
  • Omega Tech, 4909 Nautilus Court North, Boulder, CO 80301–3242, USA.
  • Monsanto, 1920 Fifth Street, Davis, CA 95616, USA.
  • Institute of Biological Chemistry, Washington State University, Pullman, WA 99164–6340, USA.
  • Sagami Chemical Research Institute (JP)
  • Washington State University (US)
Corresponding author
  • Metz, James G.
  • Roessler, Paul
  • Facciotti, Daniel
  • Lassner, Michael
  • Yamada, Akiko
  • Yazawa, Kazunaga
  • Knauf, Vic
  • Browse, John
Venue
Science
Published
2001-07-13
Volume
293
Issue
5528
Pages
290-293
ISSN
  • 0036-8075 (ISSN-L)
  • 1095-9203
OpenAlex
W2132200710
Cited by
760
References
26
Citations by year
2026: 15 · 2025: 39 · 2024: 22 · 2023: 33 · 2022: 28 · 2021: 54 · 2020: 60 · 2019: 37 · 2018: 33 · 2017: 26 · 2016: 29 · 2015: 24 · 2014: 31 · 2013: 42 · 2012: 33
FWCI
13.59
Type
journal article
Language
en
Open access
closed
Locations
  • Science (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Pharmacology › Microbial Natural Products and Biosynthesis
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
Keywords
polyunsaturated fatty acids, polyketide synthase, membrane lipids, lipid signaling, antibiotic production
MeSH terms
Anaerobiosis, Arachidonic Acids, Escherichia coli, Eukaryotic Cells, Fatty Acids, Unsaturated, Multienzyme Complexes, Open Reading Frames, Genome, Bacterial, Gammaproteobacteria, Shewanella, Fatty Acid Synthases

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