The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
A Combined Experimental and Computational Strategy to Define Protein Interaction Networks for Peptide Recognition Modules
Abstract
Peptide recognition modules mediate many protein-protein interactions critical for the assembly of macromolecular complexes. Complete genome sequences have revealed thousands of these domains, requiring improved methods for identifying their physiologically relevant binding partners. We have developed a strategy combining computational prediction of interactions from phage-display ligand consensus sequences with large-scale two-hybrid physical interaction tests. Application to yeast SH3 domains generated a phage-display network containing 394 interactions among 206 proteins and a two-hybrid network containing 233 interactions among 145 proteins. Graph theoretic analysis identified 59 highly likely interactions common to both networks. Las17 (Bee1), a member of the Wiskott-Aldrich Syndrome protein (WASP) family of actin-assembly proteins, showed multiple SH3 interactions, many of which were confirmed in vivo by coimmunoprecipitation.
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Gapped BLAST and PSI-BLAST: a new generation of protein database search programs
Altschul, S. (1997) · Nucleic Acids Research · doi:10.1093/nar/25.17.3389 · Cited by 73,302
A comprehensive analysis of protein–protein interactions in Saccharomyces cerevisiae
Uetz, P., Giot, L., Cagney, G., Mansfield, T. A., Judson, R. S., Knight, J. R., Lockshon, D., Narayan, V., Srinivasan, M., Pochart, P., et al. (2000) · Nature · doi:10.1038/35001009 · Cited by 4,706
Self-citationGuilt-by-association goes global
Oliver, S. (2000) · Nature · doi:10.1038/35001165 · Cited by 557
Cited by
Protein-Protein Interaction Detection: Methods and Analysis
Rao, V. S., Srinivas, K., Sujini, G. N., & Kumar, G. N. S. (2014) · International Journal of Proteomics · doi:10.1155/2014/147648 · Cited by 811
THE ESCRT COMPLEXES: Structure and Mechanism of a Membrane-Trafficking Network
Hurley, J. H., & Emr, S. D. (2006) · Annual Review of Biophysics and Biomolecular Structure · doi:10.1146/annurev.biophys.35.040405.102126 · Cited by 546
Linear motifs: Evolutionary interaction switches
Neduva, V., & Russell, R. B. (2005) · FEBS Letters · doi:10.1016/j.febslet.2005.04.005 · Cited by 352
Rules for Modeling Signal-Transduction Systems
Hlavacek, W. S., Faeder, J. R., Blinov, M. L., Posner, R. G., Hucka, M., & Fontana, W. (2006) · Science's STKE · doi:10.1126/stke.3442006re6 · Cited by 350
Effect of sampling on topology predictions of protein-protein interaction networks
Han, J.-D. J., Dupuy, D., Bertin, N., Cusick, M. E., & Vidal, M. (2005) · Nature Biotechnology · doi:10.1038/nbt1116 · Cited by 344
Metadata
- Authors
- Tong, A. H. Y., Drees, B., Nardelli, G., Bader, G. D., Brannetti, B., Castagnoli, L., Evangelista, M., Ferracuti, S., Nelson, B., Paoluzi, S., Quondam, M., Zucconi, A., Hogue, C. W. V., Fields, S., Boone, C., & Cesareni, G.
- Venue
- Science
- Published
- 2002-01-11
- Volume
- 295
- Issue
- 5553
- Pages
- 321-324
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2099982459
- Cited by
- 700
- References
- 3
- FWCI
- 21.38
- Type
- journal article
- Language
- en
- Open access
- closed
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- Tong, A. H. Y., Drees, B., Nardelli, G., Bader, G. D., Brannetti, B., Castagnoli, L., Evangelista, M., Ferracuti, S., Nelson, B., Paoluzi, S., Quondam, M., Zucconi, A., Hogue, C. W. V., Fields, S., Boone, C., & Cesareni, G. (2002). A Combined Experimental and Computational Strategy to Define Protein Interaction Networks for Peptide Recognition Modules. Science. https://doi.org/10.1126/science.1064987
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