The DOI matches its Crossref record; 30 of the 30 references it cites were found in a registry or an index.
Single-Molecule Speckle Analysis of Actin Filament Turnover in Lamellipodia
Abstract
Lamellipodia are thin, veil-like extensions at the edge of cells that contain a dynamic array of actin filaments. We describe an approach for analyzing spatial regulation of actin polymerization and depolymerization in vivo in which we tracked single molecules of actin fused to the green fluorescent protein. Polymerization and the lifetime of actin filaments in lamellipodia were measured with high spatial precision. Basal polymerization and depolymerization occurred throughout lamellipodia with largely constant kinetics, and polymerization was promoted within one micron of the lamellipodium tip. Most of the actin filaments in the lamellipodium were generated by polymerization away from the tip.
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Cites
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Cited by
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Mechanism of shape determination in motile cells
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Metadata
- Authors
- Watanabe, N., & Mitchison, T. J.
- Venue
- Science
- Published
- 2002-02-08
- Volume
- 295
- Issue
- 5557
- Pages
- 1083-1086
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W1996680631
- Cited by
- 483
- References
- 30
- FWCI
- 7.43
- Type
- journal article
- Language
- en
- Open access
- closed
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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