The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
Target-Selected Inactivation of the Zebrafish rag1 Gene
Abstract
The zebrafish has become a favorite organism for genetic analysis of vertebrate development, but methods for generating mutants by reverse genetic approaches have been lacking. We report a method to obtain stable mutants of a gene based on knowledge of the gene sequence only. Parental fish were mutagenized with N -ethyl- N -nitrosourea; in 2679 F 1 fish, the rag1 gene was analyzed for heterozygous mutations by resequencing. In total, we found 15 mutations: 9 resulted in amino acid substitutions and 1 resulted in a premature stop codon. This truncation mutant was found to be homozygous viable and defective in V(D)J joining. Although presumably immune deficient, these homozygous rag1 mutant fish are able to reach adulthood and are fertile. As sperm samples from all 2679 F 1 fish were collected and cryopreserved, we have in principle generated a mutant library from which mutants of most zebrafish genes can be isolated.
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Cites
Base-Calling of Automated Sequencer Traces Using Phred. II. Error Probabilities
Ewing, B., & Green, P. (1998) · Genome Research · doi:10.1101/gr.8.3.186 · Cited by 5,439
Characterization and expression of the recombination activating genes (rag1 and rag2) of zebrafish
Willett, C. E., Cherry, J. J., & Steiner, L. A. (1997) · Immunogenetics · doi:10.1007/s002510050221 · Cited by 187
Cited by
Davis, J. M., Clay, H., Lewis, J. L., Ghori, N., Herbomel, P., & Ramakrishnan, L. (2002) · Immunity · doi:10.1016/s1074-7613(02)00475-2 · Cited by 565
The ‘definitive’ (and ‘primitive’) guide to zebrafish hematopoiesis
Davidson, A. J., & Zon, L. I. (2004) · Oncogene · doi:10.1038/sj.onc.1207943 · Cited by 419
Zebrafish: A new model on the pharmaceutical catwalk
Langheinrich, U. (2003) · BioEssays · doi:10.1002/bies.10326 · Cited by 332
The Zebrafish as a Model Organism to Study Development of the Immune System
TRAVER, D., HERBOMEL, P., PATTON, E. E., MURPHEY, R. D., YODER, J. A., LITMAN, G. W., CATIC, A., AMEMIYA, C. T., ZON, L. I., & TREDE, N. S. (2003) · Advances in Immunology · doi:10.1016/s0065-2776(03)81007-6 · Cited by 276
Zebrafish as a model for the study of neutrophil biology
Henry, K. M., Loynes, C. A., Whyte, M. K. B., & Renshaw, S. A. (2013) · Journal of Leukocyte Biology · doi:10.1189/jlb.1112594 · Cited by 143
Metadata
- Authors
- Wienholds, E., Schulte-Merker, S., Walderich, B., & Plasterk, R. H. A.
- Venue
- Science
- Published
- 2002-07-05
- Volume
- 297
- Issue
- 5578
- Pages
- 99-102
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2082609114
- Cited by
- 399
- References
- 2
- FWCI
- 9.83
- Type
- journal article
- Language
- en
- Open access
- closed
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- Wienholds, E., Schulte-Merker, S., Walderich, B., & Plasterk, R. H. A. (2002). Target-Selected Inactivation of the Zebrafish rag1 Gene. Science. https://doi.org/10.1126/science.1071762
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