Cited by 741CRISPR and Genetic EngineeringDNA Repair MechanismsRNA Interference and Gene Delivery

The DOI matches its Crossref record; 10 of the 10 references it cites were found in a registry or an index.

Chimeric Nucleases Stimulate Gene Targeting in Human Cells

Porteus, Matthew H., Baltimore, David·2003·Science

Open this paper in CoChat — free account

Abstract

Correction of gene defects in human somatic cells by targeting as has been used in murine embryonic stem cells (1, 2) has been precluded by the low spontaneous rate of gene targeting (3). However, creation of a DNA double-stranded break (DSB) in the genomic target (DSB-GT) can stimulate homologous recombination by over 1000-fold (4). \n \nWe can rapidly and quantitatively measure gene targeting by correcting a mutation in a green fluorescent protein (GFP) gene that has been stably integrated into the genome (5) (fig. S1). With an optimized GFP gene targeting system, the introduction of a DSB by I–Sce I (Sce) stimulated GT >40,000-fold and the absolute rate of gene targeting reached 3 to 5% (fig. S2). Such a system, however, depends on the prior introduction of a Sce binding site into the target gene and cannot be used for endogenous genes. Chimeric nucleases (CNs) have the potential to create sequence-specific DSBs (6). CNs—fusions between zinc finger binding DNA binding domains and the endonuclease domain of Fok I—can sitespecifically cleave naked DNA in vitro (6), extrachromosomal DNA in Xenopus oocytes (7), and chromosomal DNA in Drosophila (8). CNs work as dimers, and their efficiency depends on the spacing and orientation of the zinc finger binding sites with respect to the length of the amino acid linker between the DNA binding and endonuclease domains (7, 9). \n \nQQR is an artificial zinc finger DNA binding domain that recognizes the sequence 5′-GGGGAAGAA-3′ with nanomolar affinity (10). We modified QQR chimeric nucleases (QQR-CNs) (7, 9) (Fig. 1A) and tested whether they stimulated gene targeting (Fig. 1B). The background rate of gene targeting was 0.71 events per million transfected cells (fig. S1C). QQRL18-CN stimulated gene targeting 17-fold on target QQR6 and 260-fold on target QQR8 (Fig. 1B). QQRL0-CN did not stimulate gene targeting on target QQR8, but it was as efficient as Sce in stimulating gene targeting by over 2000-fold on target QQR6 (Fig. 1B). QQRL18-CN showed some preference for an 8–base pair (bp) spacing between binding sites whereas QQRL0-CN preferred 6-bp spacing. Thus, removing the linker between the zinc spacfinger and the nuclease domains increased the activity and specificity of the fusion protein in mammalian cells. As controls, we showed that the CNs did not stimulate gene targeting if (i) they lacked a nuclear localization signal, (ii) there was a single binding site rather than an inverted repeat binding site in the target, and (iii) the cognate binding site was changed. Thus, homodimers of CNs are potent stimulators of gene targeting in human somatic cells.

Integrity signals

Registry

Crossref · 10.1126/science.1078395

Cites

10 of 10 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Altering the Genome by Homologous Recombination
Capecchi, M. R. (1989) · Science · doi:10.1126/science.2660260 · Cited by 2,037
DNA Recognition by Cys2His2 Zinc Finger Proteins
Wolfe, S. A., Nekludova, L., & Pabo, C. O. (2000) · Annual Review of Biophysics and Biomolecular Structure · doi:10.1146/annurev.biophys.29.1.183 · Cited by 1,053
Targeted Chromosomal Cleavage and Mutagenesis in Drosophila Using Zinc-Finger Nucleases
Bibikova, M., Golic, M., Golic, K. G., & Carroll, D. (2002) · Genetics · doi:10.1093/genetics/161.3.1169 · Cited by 860
Targetted correction of a mutant HPRT gene in mouse embryonic stem cells
Doetschman, T., Gregg, R., Maeda, N., Hooper, M., Melton, D., Thompson, S., & Smithies, O. (1987) · Nature · doi:10.1038/330576a0 · Cited by 756
Stimulation of Homologous Recombination through Targeted Cleavage by Chimeric Nucleases
Bibikova, M. V., Carroll, D., Segal, D. J., Trautman, J. K., Smith, J., Kim, Y., & Chandrasegaran, S. (2001) · Molecular and Cellular Biology · doi:10.1128/mcb.21.1.289-297.2001 · Cited by 614
Requirements for double-strand cleavage by chimeric restriction enzymes with zinc finger DNA-recognition domains
Smith, J. (2000) · Nucleic Acids Research · doi:10.1093/nar/28.17.3361 · Cited by 425
Genetic manipulation of genomes with rare-cutting endonucleases
Jasin, M. (1996) · Trends in Genetics · doi:10.1016/0168-9525(96)10019-6 · Cited by 391
Positive genetic selection for gene disruption in mammalian cells by homologous recombination.
Sedivy, J. M., & Sharp, P. A. (1989) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.86.1.227 · Cited by 119
Specific DNA-RNA Hybrid Binding by Zinc Finger Proteins
Shi, Y., & Berg, J. M. (1995) · Science · doi:10.1126/science.7536342 · Cited by 86
Chimeric Restriction Enzymes: What Is Next?
Chandrasegaran, S., & Smith, J. (1999) · Biological Chemistry · doi:10.1515/bc.1999.103 · Cited by 85

Flagged first, then by citations

Show all 10 references

Research works printed but not linked: 1

S. Chandrasegaran, J. Smith, Biol. Chem.380, 841 (1999).

Sorted by citations

Items 501–600 of 740

Genome Editing by Targeted Chromosomal Mutagenesis
Carroll, D. (2014) · Methods in molecular biology · doi:10.1007/978-1-4939-1862-1_1 · Cited by 9
Plant Genome Editing and its Applications in Cereals
Weinthal, D., & Gürel, F. (2016) · InTech eBooks · doi:10.5772/66818 · Cited by 9
Gene Editing in Chronic Granulomatous Disease
Sweeney, C. L., Merling, R. K., Ravin, S. S. D., Choi, U., & Malech, H. L. (2019) · Methods in molecular biology · doi:10.1007/978-1-4939-9424-3_36 · Cited by 9
TALEN-Based Chemically Inducible, Dimerization-Dependent, Sequence-Specific Nucleases
Matsumoto, D., Tamamura, H., & Nomura, W. (2019) · Biochemistry · doi:10.1021/acs.biochem.9b00798 · Cited by 9
TALEN-Based Genome Editing in Yeast
Li, T., Wright, D. A., Spalding, M. H., & Yang, B. (2014) · Fungal biology · doi:10.1007/978-3-319-10142-2_27 · Cited by 9
Genome Editing for the β-Hemoglobinopathies
Porteus, M. H. (2017) · Advances in Experimental Medicine and Biology · doi:10.1007/978-1-4939-7299-9_8 · Cited by 8
Self-citation
Isolation and tracing of matrix-producing notochordal and chondrocyte cells using ACAN-2A-mScarlet reporter human iPSC lines
Tong, X., Poramba‐Liyanage, D. W., van Hoolwerff, M., Riemers, F. M., Montilla‐Rojo, J., Warin, J., Salvatori, D. C. F., Camus, A., Meulenbelt, I., Ramos, Y. F., et al. (2024) · Science Advances · doi:10.1126/sciadv.adp3170 · Cited by 8
Progress in skin gene therapy: From the inside and out
Osborn, M. J., Panda, S., Reineke, T. M., Tolar, J., & Nyström, A. (2025) · Molecular Therapy · doi:10.1016/j.ymthe.2025.03.017 · Cited by 9
Strategies and mechanisms of precision genome engineering: From gene editing to genome writing
Huang, K., Tian, J., Zhao, W., Zhang, S., Xiang, L., Yang, M., Ls, L., Huang, Y., Zhang, N., Sun, L., et al. (2026) · iMetaOmics. · doi:10.1002/imo2.70115 · Cited by 8
Knockout punches with a fistful of zinc fingers
Wilson, J. H. (2008) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0802298105 · Cited by 8
From Agrobacterium to viral vectors: genome modification of plant cells by rare cutting restriction enzymes
Marton, I., Honig, A., Omid, A., Costa, N. D., Marhevka, E., Cohen, B. A., Zuker, A., & Vainstein, A. (2013) · The International Journal of Developmental Biology · doi:10.1387/ijdb.130205av · Cited by 8
Targeted genome correction by a single adenoviral vector simultaneously carrying an inducible zinc finger nuclease and a donor template
Zhang, W., Chen, H., Zheng, X., Wang, D., Ji, H., Xia, H., & Mao, Q. (2014) · Journal of Biotechnology · doi:10.1016/j.jbiotec.2014.08.003 · Cited by 8
Analysis of conventional and alternative CRISPR/Cas9 genome editing to enhance a single-base pair knock-in mutation
Edmondson, C., Zhou, Q., & Liu, X. (2021) · BMC Biotechnology · doi:10.1186/s12896-021-00707-5 · Cited by 10
Developing CRISPR/Cas9-Mediated Fluorescent Reporter Human Pluripotent Stem-Cell Lines for High-Content Screening
Vojnits, K., Nakanishi, M., Porras, D. P., Kim, Y., Feng, Z., Golubeva, D., & Bhatia, M. (2022) · Molecules · doi:10.3390/molecules27082434 · Cited by 10
CRISPR/Cas9-Directed Gene Trap Constitutes a Selection System for Corrected BCR/ABL Leukemic Cells in CML
Vuelta, E., Ordóñez, J. L., Sanz, D. J., Ballesteros, S., Hernández‐Rivas, J. M., Méndez-Sánchez, L., Sánchez‐Martín, M., & García‐Tuñón, I. (2022) · International Journal of Molecular Sciences · doi:10.3390/ijms23126386 · Cited by 8
Engineered Zinc Finger Nucleases for Targeted Genome Editing
Ramirez, C. L., & Joung, J. K. (2012) · Topics in current genetics · doi:10.1007/978-94-007-4531-5_5 · Cited by 7
Genome Editing: Advances and Prospects
Patel, J., & Mishra, A. (2019) · Springer eBooks · doi:10.1007/978-981-13-8499-8_7 · Cited by 8
A comparison of synthetic oligodeoxynucleotides, DNA fragments and AAV-1 for targeted episomal and chromosomal gene repair
Leclerc, X., Danos, O., Scherman, D., & Kichler, A. (2009) · BMC Biotechnology · doi:10.1186/1472-6750-9-35 · Cited by 8
Engineering and Applications of Chimeric Nucleases
Kandavelou, K., Mani, M., Durai, S., & Chandrasegaran, S. (2004) · Nucleic acids and molecular biology · doi:10.1007/978-3-642-18851-0_16 · Cited by 8
Engineering Cys2His2 Zinc Finger Domains Using a Bacterial Cell-Based Two-Hybrid Selection System
Thibodeau-Beganny, S., & Joung, J. K. (2007) · Methods in molecular biology · doi:10.1007/978-1-59745-547-3_17 · Cited by 8
CRISPR Technology Acts as a Dual-Purpose Tool in Pig Breeding: Enhancing Both Agricultural Productivity and Biomedical Applications
Fu, B., Ma, H., Huo, X., Zhu, Y. J., & Liu, D. (2024) · Biomolecules · doi:10.3390/biom14111409 · Cited by 9
Cell Line Development
Häuser, H. (2014) · Cell engineering · doi:10.1007/978-3-319-10320-4_1 · Cited by 7
CRISPR-Cas systems for genome editing of mammalian cells
Mani, I., Arazoe, T., & Singh, V. (2021) · Progress in molecular biology and translational science · doi:10.1016/bs.pmbts.2021.01.011 · Cited by 9
Multiplexed bioluminescence-mediated tracking of DNA double-strand break repairs in vitro and in vivo
Chien, J. C.-Y., Badr, C. E., & Lai, C. P. (2021) · Nature Protocols · doi:10.1038/s41596-021-00564-8 · Cited by 6
Nutrigenomics in Cereals
Yadav, S. K., Yadav, P. D., & Chinnusamy, V. (2023) · Springer eBooks · doi:10.1007/978-981-19-4308-9_12 · Cited by 7
Inverted terminal repeats of adeno-associated virus decrease random integration of a gene targeting fragment in Saccharomyces cerevisiae
Galli, A., & Cervelli, T. (2014) · BMC Molecular Biology · doi:10.1186/1471-2199-15-5 · Cited by 7
Multiple copies of a linear donor fragment released in situ from a vector improve the efficiency of zinc-finger nuclease-mediated genome editing
Zhang, W., Wang, D., Liu, S., Zheng, X., Ji, H. J., Xia, H., & Mao, Q. (2014) · Gene Therapy · doi:10.1038/gt.2013.83 · Cited by 7
Enhanced gene disruption by programmable nucleases delivered by a minicircle vector
Dad, A.-B., Ramakrishna, S., Song, M., & Kim, S. (2014) · Gene Therapy · doi:10.1038/gt.2014.76 · Cited by 7
Evaluating the Mutagenic Activity of Targeted Endonucleases Containing a Sharkey FokI Cleavage Domain Variant in Zebrafish
Pillay, L. M., Selland, L. G., Fleisch, V. C., Leighton, P. L. A., Cheng, C. S., Famulski, J. K., Ritzel, R. G., March, L. D., Wang, H., Allison, W. T., et al. (2013) · Zebrafish · doi:10.1089/zeb.2012.0832 · Cited by 7
Genome Editing in Rats Using TALE Nucleases
Tesson, L., Remy, S., Ménoret, S., Usal, C., Thinard, R., Savignard, C., Cian, A. D., Giovannangeli, C., Concordet, J., & Anegón, I. (2015) · Methods in molecular biology · doi:10.1007/978-1-4939-2932-0_18 · Cited by 7
Synergizing CRISPR-Cas9 with Advanced Artificial Intelligence and Machine Learning for Precision Drug Delivery: Technological Nexus and Regulatory Insights
Roy, A. A., Pokale, R., Mukharya, A., Nikam, A. N., Dua, K., Rao, B. S. S., Seetharam, R. N., & Mutalik, S. (2025) · Current Gene Therapy · doi:10.2174/0115665232342293241120033251 · Cited by 7
Molecular Detection Methods for Forest Pathogens
Wu, J. C., Dai, T., Wang, X., & Wang, Y. (2025) · Critical Reviews in Plant Sciences · doi:10.1080/07352689.2025.2489776 · Cited by 6
RGEN-seq for highly sensitive amplification-free screen of off-target sites of gene editors
Kuzin, A., Redler, B., Onuska, J., & Slesarev, A. I. (2021) · Scientific Reports · doi:10.1038/s41598-021-03160-8 · Cited by 6
SARS-CoV-2 subgenomic RNA: formation process and rapid molecular diagnostic methods
Ge, X., Zhou, H., Shen, F., Yang, G., Zhang, Y., Zhang, X., & Li, H. (2023) · Clinical Chemistry and Laboratory Medicine (CCLM) · doi:10.1515/cclm-2023-0846 · Cited by 7
Omics and CRISPR in CHO bioproduction: a state-of-the-art review
Sorourian, S., Behzad‐Behbahani, A., Dehbidi, G. R., Zare, F., Farajnia, S., Najafi, H., & Safari, F. (2024) · Biotechnology and Bioprocess Engineering · doi:10.1007/s12257-024-00026-4 · Cited by 6
Seeing the light: integrating genome engineering with double-strand break repair
Porteus, M. H. (2011) · Nature Methods · doi:10.1038/nmeth.1656 · Cited by 6
Self-citation
An artificial restriction DNA cutter for site-selective gene insertion in human cells
Ito, K., Shigi, N., & Komiyama, M. (2013) · Chemical Communications · doi:10.1039/c3cc43261k · Cited by 6
Minireview: Genome Editing of Human Pluripotent Stem Cells for Modeling Metabolic Disease
Yu, H., & Cowan, C. A. (2016) · Molecular Endocrinology · doi:10.1210/me.2015-1290 · Cited by 6
Generation of Knock-in Mouse by Genome Editing
Fujii, W. (2017) · Methods in molecular biology · doi:10.1007/978-1-4939-7128-2_8 · Cited by 7
Genome Editing in Higher Plants
Osakabe, Y., & Osakabe, K. (2014) · Springer eBooks · doi:10.1007/978-4-431-55227-7_13 · Cited by 6
Treatment of GATA2 deficiency by allele-specific CRISPR-Cas9-directed gene correction in hematopoietic stem cells
Skov, T. W., Wolff, J. H., Haslund, D., Revenfeld, A. L., van de Venn, L., Dorset, S. R., Ravendran, S., Jørgensen, S. E., Thomsen, M. K., Holm, M., et al. (2025) · Molecular Therapy · doi:10.1016/j.ymthe.2025.07.038 · Cited by 6
Viral delivery of genome-modifying proteins for cellular reprogramming
Mikkelsen, J. G. (2018) · Current Opinion in Genetics & Development · doi:10.1016/j.gde.2018.06.005 · Cited by 6
Engineered Meganucleases for Genome Engineering Purposes
Epinat, J. C., Silva, G. H., Pâques, F., Smith, J. N. P., & Duchateau, P. N. (2012) · Topics in current genetics · doi:10.1007/978-94-007-4531-5_6 · Cited by 5
A System for the Measurement of Gene Targeting Efficiency in Human Cell Lines Using an Antibiotic Resistance—GFP Fusion Gene
Konishi, Y., Karnan, S., Takahashi, M., Ota, A., Damdindorj, L., Hosokawa, Y., & Konishi, H. (2012) · BioTechniques · doi:10.2144/0000113911 · Cited by 5
Therapeutic Gene Editing with CRISPR
Hahn, E., & Hiemenz, M. (2020) · Clinics in Laboratory Medicine · doi:10.1016/j.cll.2020.02.008 · Cited by 5
Modern Tools of Genome Engineering and Their Applications
Kaur, R., Singh, A. K., Singh, D. K., & Singh, S. (2023) · Springer eBooks · doi:10.1007/978-981-99-3126-2_9 · Cited by 5
The Development and Use of Zinc-Finger Nucleases
Carroll, D. (2016) · Advances in experimental medicine and biology · doi:10.1007/978-1-4939-3509-3_2 · Cited by 5
A Possible Approach for Stem Cell Gene Therapy of Fanconi Anemia
Song, L. (2009) · Current Gene Therapy · doi:10.2174/156652309787354667 · Cited by 5
Improving gene replacement by intracellular formation of linear homologous DNA
de Piédoue, G., Maurisse, R., Kuzniak, I., López, B. S., Perrin, A., Nègre, O., Leboulch, P., & Feugeas, J. (2005) · The Journal of Gene Medicine · doi:10.1002/jgm.706 · Cited by 5
EDITORIAL: "AUTOLOGOUS STEM CELL GENE THERAPY: TOWARD A UNIVERSAL PLATFORM FOR PERSONALIZED THERAPY"
Bertolotti, R. (2006) · Gene Therapy and Regulation · doi:10.1142/s1568558607000022 · Cited by 5
Creating Zinc Finger Nucleases to Manipulate the Genome in a Site-Specific Manner Using a Modular-Assembly ApproachPreprint
Porteus, M. H. (2010) · Cold Spring Harbor Protocols · doi:10.1101/pdb.top93 · Cited by 5
Self-citation
Cysteine-rich zinc finger proteins and the nuclear factor kappa-B pathway
Stoltzfus, A. T., & Michel, S. L. J. (2024) · Frontiers in Chemical Biology · doi:10.3389/fchbi.2024.1503390 · Cited by 4
Genetic Modification Stimulated by the Induction of a Site-Specific Break Distant from the Locus of Correction in Haploid and Diploid Yeast Saccharomyces cerevisiae
Stuckey, S., & Storici, F. (2014) · Methods in molecular biology · doi:10.1007/978-1-62703-761-7_20 · Cited by 4
Gene Editing in One‐Cell Embryos by Zinc‐Finger and TAL Nucleases
Wefers, B., Meyer, M., Hensler, S.-V., Panda, S. K., Ortiz, O., Wurst, W., & Kühn, R. (2012) · Current Protocols in Mouse Biology · doi:10.1002/9780470942390.mo120177 · Cited by 4
Rescue the Failed Half-ZFN by a Sensitive Mammalian Cell-Based Luciferase Reporter System
Zhang, W., Guo, Y., Zhang, C., Ji, H., Meng, W., Wang, D., Li, X., Mao, Q., & Xia, H. (2012) · PLoS ONE · doi:10.1371/journal.pone.0045169 · Cited by 4
Gene editing based hearing impairment research and therapeutics
Kang, W., Sun, Z., Zhao, X., Wang, X., Tao, Y., & Wu, H. (2019) · Neuroscience Letters · doi:10.1016/j.neulet.2019.134326 · Cited by 4
Profiling Genome-Wide Specificity of CRISPR-Cas9 Using Digenome-Seq
Kim, D., & Kim, J. (2020) · Methods in molecular biology · doi:10.1007/978-1-0716-0687-2_13 · Cited by 4
Application in gene therapy and DNA/RNA vaccines
Limeres, M. J., Toscanini, M. A., Devoto, T. B., Ladetto, M. F., Castro, G. R., & Cuestas, M. L. (2022) · Elsevier eBooks · doi:10.1016/b978-0-323-91229-7.00001-5 · Cited by 4
ENGINEERING HOMING ENDONUCLEASES TO MODIFY COMPLEX GENOMES
Gimble, F. S. (2006) · Gene Therapy and Regulation · doi:10.1142/s1568558607000034 · Cited by 4
CRISPR/Cas9 Gene Targeting in Primary Mouse Bone Marrow-Derived Macrophages
Bailis, W. (2019) · Methods in molecular biology · doi:10.1007/978-1-0716-0203-4_14 · Cited by 4
Efficient in vivo genome editing mediated by stem cells-derived extracellular vesicles carrying designer nucleasesPreprint
Bobis‐Wozowicz, S., Kania, K., Nit, K., Błażowska, N., Kmiotek‐Wasylewska, K., Paw, M., Karnas, E., Szyposzyńska, A., Tyszka‐Czochara, M., Woźnicka, O., et al. (2021) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2021.02.25.432823 · Cited by 4
Genome Editing in Somatic Cells Using Zinc Finger Nucleases and Transcription Activator-Like Effector Nucleases
Pruett‐Miller, S. M. (2013) · Elsevier eBooks · doi:10.1016/b978-0-12-386541-0.00029-1 · Cited by 3
Genome Editing for Neuromuscular Diseases
Ousterout, D. G., & Gersbach, C. A. (2016) · Advances in experimental medicine and biology · doi:10.1007/978-1-4939-3509-3_4 · Cited by 4
Current Status of Genome Editing in Cardiovascular Medicine
Termglinchan, V., Karakikes, I., Seeger, T., & Wu, J. C. (2016) · Springer eBooks · doi:10.1007/978-3-319-34148-4_7 · Cited by 3
Stable Cells with NF-κB-ZsGreen Fused Genes Created by TALEN Editing and Homology Directed Repair for Screening Anti-inflammation Drugs
Zhang, S., Luo, T., & Wang, J. (2021) · Journal of Inflammation Research · doi:10.2147/jir.s298938 · Cited by 3
Comprehensive optimization of a reporter assay toolbox for three distinct CRISPR‐Cas systems
Chen, L., Gao, H., Zhou, B., & Wang, Y. (2021) · FEBS Open Bio · doi:10.1002/2211-5463.13198 · Cited by 3
Xenotransplantation: The Contribution of CRISPR/Cas9 Gene Editing Technology
Stewart, Z. A. (2022) · Current Transplantation Reports · doi:10.1007/s40472-022-00380-3 · Cited by 3
An efficient and cost-effective method for disrupting genes in RAW264.7 macrophages using CRISPR-Cas9
Hossain, J., & O’Connor, T. J. (2024) · PLoS ONE · doi:10.1371/journal.pone.0299513 · Cited by 3
Signaling Molecules: Importance in Health and Disease Conditions
Banerjee, J., Limaye, D., Pathan, A., Banerjee, S., & Tiwari, A. K. (2025) · The Receptors · doi:10.1007/978-3-031-81991-9_2 · Cited by 5
Plant Genome Engineering Using Zinc Finger Nucleases
Kumar, S., & Thompson, W. F. (2009) · Springer eBooks · doi:10.1007/978-90-481-2967-6_24 · Cited by 3
Recent Advances and Applications of Transgenic Animal Technology
Miao, X. (2012) · InTech eBooks · doi:10.5772/38040 · Cited by 3
Genetic Manipulation of Human Embryonic Stem Cells
Drukker, M., & Benvenisty, N. (2003) · Humana Press eBooks · doi:10.1007/978-1-59259-423-8_14 · Cited by 3
Genetic Analysis of Synaptogenesis
Lu, C. S., & Vactor, D. V. (2013) · Elsevier eBooks · doi:10.1016/b978-0-12-397266-8.00104-6 · Cited by 3
A promoter trap vector for knocking out bovine myostatin gene with high targeting efficiency
Zhao, L. H., Zhao, Y., Liang, H., Yun, T., Han, X., Zhang, M. L., Zhou, X., Hou, D.-X., Li, R. F., & Li, X. L. (2015) · Genetics and Molecular Research · doi:10.4238/2015.march.31.5 · Cited by 3
Epigenetic Modification of the Repair Donor Regulates Targeted Gene Correction
Humbert, O., & Maizels, N. (2012) · Molecular Therapy — Nucleic Acids · doi:10.1038/mtna.2012.42 · Cited by 3
Genetic modification of hematopoietic stem cells: recent advances in the gene therapy of inherited diseases
Bueren, J. A., Güenechea, G., Casado, J. A., Lamana, M. L., & Segovia, J. C. (2003) · Archives of Medical Research · doi:10.1016/j.arcmed.2003.09.004 · Cited by 3
Biosafety and knowledge gaps of site directed nucleases and oligonucleotide-directed mutagenesis
Agapito-Tenfen, S. Z., & Wikmark, O.-G. (2015) · Cited by 3
Manufacturing and Delivering Genome-Editing Proteins
Liu, J., Liang, Y.-J., Ren, P.-L., & Gaj, T. (2018) · Methods in molecular biology · doi:10.1007/978-1-4939-8799-3_19 · Cited by 3
Site-specific Manipulation of Mitochondrial DNA by Artificial Restriction DNA Cutter
Harumoto, T., Shigi, N., Tsumoto, K., & Komiyama, M. (2019) · Chemistry Letters · doi:10.1246/cl.190572 · Cited by 3
What history tells us XL. The success story of the expression ‘genome editing’
Morange, M. (2016) · Journal of Biosciences · doi:10.1007/s12038-016-9597-x · Cited by 3
Gene targeting using zinc finger
Carroll, D. (2005) · Cited by 3
The convergence of synthetic biology and metagenomics: Current advances and future perspectives
Maqbool, M., Haque, E., Thacharodi, A., Meenatchi, R., Arafath, A. Y., Fathima, S. A., Genevieve, K. K., Singh, T., Rajalakshmi, D., Nisha, K., et al. (2026) · Gene Reports · doi:10.1016/j.genrep.2026.102506 · Cited by 3
Modeling rare pediatric neurogenetic disorders with IPSCs
Kim, J., Nonis, D., Otero, M. G., & Pierson, T. M. (2019) · AIMS Cell and Tissue Engineering · doi:10.3934/celltissue.2019.1.1 · Cited by 2
Gene Editing and Human iPSCs in Cardiovascular and Metabolic Diseases
Giallongo, S., Re, O. L., Resnick, I. B., Raffaele, M., & Vinciguerra, M. (2022) · Advances in experimental medicine and biology · doi:10.1007/978-981-19-5642-3_18 · Cited by 2
Recent Advances and Improvements in the Biosafety of Gene Therapy
Sivalingam, J., & Lian, O. (2011) · InTech eBooks · doi:10.5772/21733 · Cited by 3
Progress and Challenges in AAV-Mediated Gene Therapy for Duchenne Muscular Dystrophy
Takeda, S., & Ok, T. (2011) · InTech eBooks · doi:10.5772/18624 · Cited by 2
Unsuccessful attempt at gene-editing by homologous recombination in the zebrafish germ line using the approach of “Rong and Golic”
Brookfield, R., Dafhnis-Calas, F., Xu, Z., & Brown, W. R. (2012) · Transgenic Research · doi:10.1007/s11248-012-9607-1 · Cited by 2
Flowing through the CRISPR-CAScade: Will genome editing boost cell therapies?
Ben‐David, U. (2013) · Molecular and Cellular Therapies · doi:10.1186/2052-8426-1-3 · Cited by 2
Homing Endonuclease Target Site Specificity Defined by Sequential Enrichment and Next-Generation Sequencing of Highly Complex Target Site Libraries
Li, H., & Monnat, R. J. (2014) · Methods in molecular biology · doi:10.1007/978-1-62703-968-0_12 · Cited by 2
Strategies to Determine Off-Target Effects of Engineered Nucleases
Fine, E. J., Cradick, T. J., & Bao, G. (2016) · Advances in experimental medicine and biology · doi:10.1007/978-1-4939-3509-3_11 · Cited by 2
Induced Pluripotent Stem Cells for the Treatment of Hemophilia A
Joseph, B. C. (2014) · Journal of Cell Science & Therapy · doi:10.4172/2157-7013.1000e119 · Cited by 2
4.32 Gene Editing Tools
Ede, D., Farhang, N., Stover, J. D., & Bowles, R. D. (2017) · Elsevier eBooks · doi:10.1016/b978-0-12-803581-8.09290-0 · Cited by 2
Genome Editing by Programmable Nucleases and their applications in livestock species
Zafar, I., Singh, S., & Kumar, J. (2019) · Journal of Livestock Science · doi:10.33259/jlivestsci.2019.32-47 · Cited by 2
Somatic Cell Nuclear Transfer in Zebrafish
Siripattarapravat, K., Pinmee, B., & Cibelli, J. B. (2013) · Elsevier eBooks · doi:10.1016/b978-0-12-386541-0.00016-3 · Cited by 2
Mitochondrially targeted zinc finger nucleases
Pinheiro, P. F., Gammage, P. A., & Minczuk, M. A. (2020) · Elsevier eBooks · doi:10.1016/b978-0-12-819656-4.00019-x · Cited by 2
Zinc Finger Nuclease-Mediated Gene Targeting in Plants
Petolino, J. F., Sastry, L., & Samuel, J. P. (2015) · Springer eBooks · doi:10.1007/978-1-4939-2202-4_12 · Cited by 2
Omics in Stem Cell Therapy: The Road Ahead
Byun, K., Jeong, G., Collingwood, T. N., & Lee, B. (2012) · Humana Press eBooks · doi:10.1007/978-1-61779-940-2_13 · Cited by 2
Defeating Randomness – Targeted Integration as a Boost for Biotechnology
Gama-Norton, L., Riemer, P., Sandhu, U., Nehlsen, K., Schucht, R., Häuser, H., & Wirth, D. (2009) · Cell engineering · doi:10.1007/978-90-481-2245-5_3 · Cited by 3
The mechanism and application of zinc finger nucleases
Zhong, Q., & Zhao, S. (2011) · Hereditas (Beijing) · doi:10.3724/sp.j.1005.2011.00123 · Cited by 2
19 DIRECT INTRODUCTION OF GENE CONSTRUCTS INTO THE PRONUCLEUS-LIKE STRUCTURE OF CLONED EMBRYOS: A NEW STRATEGY FOR THE GENERATION OF GENETICALLY MODIFIED PIGS
Kurome, M., Leuchs, S., Keßler, B., Kemter, E., Jemiller, E., Zakhartchenko, V., & Wolf, E. (2015) · Reproduction Fertility and Development · doi:10.1071/rdv28n2ab19 · Cited by 2

Authors: Porteus, M. H., & Baltimore, D.
Affiliations: California Institute of Technology (US)
Corresponding author: Porteus, Matthew H.
Baltimore, David
ORCID: Porteus, Matthew H.
Baltimore, David
Venue: Science
Publisher: American Association for the Advancement of Science (AAAS)
Published: 2003-05-02
Volume: 300
Issue: 5620
Pages: 763-763
ISSN: 0036-8075 (ISSN-L)
1095-9203
DOI: 10.1126/science.1078395
OpenAlex: W2051395442
Cited by: 741
References: 10
Citations by year: 2026: 8 · 2025: 18 · 2024: 25 · 2023: 16 · 2022: 26 · 2021: 30 · 2020: 34 · 2019: 33 · 2018: 27 · 2017: 28 · 2016: 52 · 2015: 45 · 2014: 69 · 2013: 41 · 2012: 51
FWCI: 9.12
Type: journal article
Language: en
Open access: closed
Locations: Science (publishedVersion)
PubMed (publishedVersion)
CaltechAUTHORS (California Institute of Technology) (submittedVersion)
Funders: National Institute of General Medical Sciences (R01GM039458, R01 GM39458)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
Keywords: gene targeting, homologous recombination, zinc finger DNA-binding domain, DNA double-strand breaks, linker length, somatic cell therapy, gene therapy vectors, somatic cell hybrids, somatic cell nuclear transfer
MeSH terms: Cell Line, DNA, Humans, Luminescent Proteins, Mutation, Recombinant Fusion Proteins, Recombination, Genetic, Transfection, Deoxyribonucleases, Type II Site-Specific, Zinc Fingers, Gene Targeting, Dimerization, Nuclear Localization Signals, Saccharomyces cerevisiae Proteins, Green Fluorescent Proteins

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

Chimeric Nucleases Stimulate Gene Targeting in Human Cells

Abstract

Overview

Integrity signals

Verification

Claims the paper makes

Claims other papers make about this one

Citations

Cites

Cited by

Related

Metadata

Record

About these checks