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Transcription Elongation Factors Repress Transcription Initiation from Cryptic Sites
Abstract
Previous studies have suggested that transcription elongation results in changes in chromatin structure. Here we present studies of Saccharomyces cerevisiae Spt6, a conserved protein implicated in both transcription elongation and chromatin structure. Our results show that, surprisingly, an spt6 mutant permits aberrant transcription initiation from within coding regions. Furthermore, transcribed chromatin in the spt6 mutant is hypersensitive to micrococcal nuclease, and this hypersensitivity is suppressed by mutational inactivation of RNA polymerase II. These results suggest that Spt6 plays a critical role in maintaining normal chromatin structure during transcription elongation, thereby repressing transcription initiation from cryptic promoters. Other elongation and chromatin factors, including Spt16 and histone H3, appear to contribute to this control.
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Citations
Cited by
The complex language of chromatin regulation during transcription
Berger, S. L. (2007) · Nature · doi:10.1038/nature05915 · Cited by 2,768
Functions of Site-Specific Histone Acetylation and Deacetylation
Shahbazian, M. D., & Grunstein, M. (2007) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.76.052705.162114 · Cited by 1,635
Structure of activated transcription complex Pol II–DSIF–PAF–SPT6
Vos, S. M., Farnung, L., Boehning, M., Wigge, C., Linden, A., Urlaub, H., & Cramer, P. (2018) · Nature · doi:10.1038/s41586-018-0440-4 · Cited by 432
Distinction and Relationship between Elongation Rate and Processivity of RNA Polymerase II In Vivo
Mason, P. B., & Struhl, K. (2005) · Molecular Cell · doi:10.1016/j.molcel.2005.02.017 · Cited by 359
Adkins, M. W., Howar, S. R., & Tyler, J. K. (2004) · Molecular Cell · doi:10.1016/j.molcel.2004.05.016 · Cited by 294
Metadata
- Authors
- Kaplan, C. D., Laprade, L., & Winston, F.
- Venue
- Science
- Published
- 2003-08-22
- Volume
- 301
- Issue
- 5636
- Pages
- 1096-1099
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2085759022
- Cited by
- 585
- References
- 0
- FWCI
- 8.90
- Type
- journal article
- Language
- en
- Open access
- closed
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