The DOI matches its Crossref record; 26 of the 26 references it cites were found in a registry or an index.
Dynamic Instability in a DNA-Segregating Prokaryotic Actin Homolog
Abstract
Dynamic instability—the switching of a two-state polymer between phases of steady elongation and rapid shortening—is essential to the cellular function of eukaryotic microtubules, especially during chromosome segregation. Since the discovery of dynamic instability 20 years ago, no other biological polymer has been found to exhibit this behavior. Using total internal reflection fluorescence microscopy and fluorescence resonance energy transfer, we observe that the prokaryotic actin homolog ParM, whose assembly is required for the segregation of large, low–copy number plasmids, displays both dynamic instability and symmetrical, bidirectional polymerization. The dynamic instability of ParM is regulated by adenosine triphosphate (ATP) hydrolysis, and filaments are stabilized by a cap of ATP-bound monomers. ParM is not related to tubulin, so its dynamic instability must have arisen by convergent evolution driven by a set of common constraints on polymer-based segregation of DNA.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Dynamic instability of microtubule growth
Mitchison, T., & Kirschner, M. (1984) · Nature · doi:10.1038/312237a0 · Cited by 3,337
A theory of linear and helical aggregations of macromolecules
Oosawa, F., & Kasai, M. (1962) · Journal of Molecular Biology · doi:10.1016/s0022-2836(62)80112-0 · Cited by 766
Cellular Control of Actin Nucleation
Welch, M. D., & Mullins, R. D. (2002) · Annual Review of Cell and Developmental Biology · doi:10.1146/annurev.cellbio.18.040202.112133 · Cited by 517
Self-citationAn actin-like gene can determine cell polarity in bacteria
Gitai, Z., Dye, N., & Shapiro, L. (2004) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0402638101 · Cited by 306
Dysfunctional MreB inhibits chromosome segregation in Escherichia coli
Kruse, T. (2003) · The EMBO Journal · doi:10.1093/emboj/cdg504 · Cited by 276
Mitosis: a history of division
Mitchison, T. J., & Salmon, E. D. (2001) · Nature Cell Biology · doi:10.1038/35050656 · Cited by 275
Formin-induced nucleation of actin filaments
Zigmond, S. H. (2004) · Current Opinion in Cell Biology · doi:10.1016/j.ceb.2003.10.019 · Cited by 273
Mechanism of Formin-Induced Nucleation of Actin Filaments
Pring, M., Evangelista, M., Boone, C. M., Yang, C., & Zigmond, S. H. (2002) · Biochemistry · doi:10.1021/bi026520j · Cited by 260
Prokaryotic DNA segregation by an actin-like filament
Moller-Jensen, J. (2002) · The EMBO Journal · doi:10.1093/emboj/cdf320 · Cited by 253
F-actin-like filaments formed by plasmid segregation protein ParM
van den Ent, F. (2002) · The EMBO Journal · doi:10.1093/emboj/cdf672 · Cited by 245
Cited by
Bacterial Growth and Cell Division: a Mycobacterial Perspective
Hett, E. C., & Rubin, E. J. (2008) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.00028-07 · Cited by 408
Shih, Y.-L., & Rothfield, L. (2006) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.00017-06 · Cited by 266
The Cytoskeleton and Its Regulation by Calcium and Protons
Hepler, P. K. (2016) · Plant Physiology · doi:10.1104/pp.15.01506 · Cited by 133
Popp, D., Narita, A., Oda, T., Fujisawa, T., Matsuo, H., Nitanai, Y., Iwasa, M., Maeda, K., Onishi, H., & Maéda, Y. (2008) · The EMBO Journal · doi:10.1038/sj.emboj.7601978 · Cited by 84
Origin and Evolution of the Self-Organizing Cytoskeleton in the Network of Eukaryotic Organelles
Jekely, G. (2014) · Cold Spring Harbor Perspectives in Biology · doi:10.1101/cshperspect.a016030 · Cited by 38
Metadata
- Authors
- Garner, E. C., Campbell, C. S., & Mullins, R. D.
- Venue
- Science
- Published
- 2004-11-05
- Volume
- 306
- Issue
- 5698
- Pages
- 1021-1025
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2019721726
- Cited by
- 274
- References
- 26
- FWCI
- 8.55
- Type
- journal article
- Language
- en
- Open access
- closed
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Dynamic Instability in a DNA-Segregating Prokaryotic Actin Homolog. https://app.cochat.ai/papers/10.1126/science.1101313
- The paper
- Garner, E. C., Campbell, C. S., & Mullins, R. D. (2004). Dynamic Instability in a DNA-Segregating Prokaryotic Actin Homolog. Science. https://doi.org/10.1126/science.1101313
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
