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Genome Sequence of the PCE-Dechlorinating Bacterium Dehalococcoides ethenogenes

Seshadri, Rekha, Adrian, Lorenz, Fouts, Derrick E., Eisen, Jonathan A., Phillippy, Adam M., Methe, Barbara A., Ward, Naomi L., Nelson, William C., Deboy, Robert T., Khouri, Hoda M., Kolonay, James F., Dodson, Robert J., Daugherty, Sean C., Brinkac, Lauren M., Sullivan, Steven A., Madupu, Ramana, Nelson, Karen E., Kang, Katherine H., Impraim, Marjorie, Tran, Kevin, Robinson, Jeffrey M., Forberger, Heather A., Fraser, Claire M., Zinder, Stephen H., Heidelberg, John F.·2005·Science

Abstract

Dehalococcoides ethenogenes is the only bacterium known to reductively dechlorinate the groundwater pollutants, tetrachloroethene (PCE) and trichloroethene, to ethene. Its 1,469,720–base pair chromosome contains large dynamic duplicated regions and integrated elements. Genes encoding 17 putative reductive dehalogenases, nearly all of which were adjacent to genes for transcription regulators, and five hydrogenase complexes were identified. These findings, plus a limited repertoire of other metabolic modes, indicate that D. ethenogenes is highly evolved to utilize halogenated organic compounds and H 2. Diversification of reductive dehalogenase functions appears to have been mediated by recent genetic exchange and amplification. Genome analysis provides insights into the organism's complex nutrient requirements and suggests that an ancestor was a nitrogen-fixing autotroph.

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Metadata

Authors
Seshadri, R., Adrian, L., Fouts, D. E., Eisen, J. A., Phillippy, A. M., Methe, B. A., Ward, N. L., Nelson, W. C., Deboy, R. T., Khouri, H. M., Kolonay, J. F., Dodson, R. J., Daugherty, S. C., Brinkac, L. M., Sullivan, S. A., Madupu, R., Nelson, K. E., Kang, K. H., Impraim, M., Tran, K., Robinson, J. M., Forberger, H. A., Fraser, C. M., Zinder, S. H., & Heidelberg, J. F.
Venue
Science
Published
2005-01-07
Volume
307
Issue
5706
Pages
105-108
ISSN
  • 0036-8075 (ISSN-L)
  • 1095-9203
OpenAlex
W2170525977
Cited by
435
References
1
FWCI
41.63
Type
journal article
Language
en
Open access
closed

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CoChat. (2026). Citation check: Genome Sequence of the PCE-Dechlorinating Bacterium Dehalococcoides ethenogenes. https://app.cochat.ai/papers/10.1126/science.1102226
The paper
Seshadri, R., Adrian, L., Fouts, D. E., Eisen, J. A., Phillippy, A. M., Methe, B. A., Ward, N. L., Nelson, W. C., Deboy, R. T., Khouri, H. M., Kolonay, J. F., Dodson, R. J., Daugherty, S. C., Brinkac, L. M., Sullivan, S. A., Madupu, R., Nelson, K. E., Kang, K. H., Impraim, M., . . . Heidelberg, J. F. (2005). Genome Sequence of the PCE-Dechlorinating Bacterium Dehalococcoides ethenogenes. Science. https://doi.org/10.1126/science.1102226

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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.