The DOI matches its Crossref record.
Reconstitution of Circadian Oscillation of Cyanobacterial KaiC Phosphorylation in Vitro
Abstract
Kai proteins globally regulate circadian gene expression of cyanobacteria. The KaiC phosphorylation cycle, which persists even without transcription or translation, is assumed to be a basic timing process of the circadian clock. We have reconstituted the self-sustainable oscillation of KaiC phosphorylation in vitro by incubating KaiC with KaiA, KaiB, and adenosine triphosphate. The period of the in vitro oscillation was stable despite temperature change (temperature compensation), and the circadian periods observed in vivo in KaiC mutant strains were consistent with those measured in vitro. The enigma of the circadian clock can now be studied in vitro by examining the interactions between three Kai proteins.
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Citations
Cited by
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Dibner, C., Schibler, U., & Albrecht, U. (2010) · Annual Review of Physiology · doi:10.1146/annurev-physiol-021909-135821 · Cited by 2,421
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Bass, J., & Takahashi, J. S. (2010) · Science · doi:10.1126/science.1195027 · Cited by 1,910
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Peroxiredoxins are conserved markers of circadian rhythms
Edgar, R. S., Green, E. W., Zhao, Y., van Ooijen, G., Olmedo, M., Qin, X., Xu, Y., Pan, M., Valekunja, U. K., Feeney, K. A., et al. (2012) · Nature · doi:10.1038/nature11088 · Cited by 914
Metadata
- Authors
- Nakajima, M., Imai, K., Ito, H., Nishiwaki, T., Murayama, Y., Iwasaki, H., Oyama, T., & Kondo, T.
- Venue
- Science
- Published
- 2005-04-15
- Volume
- 308
- Issue
- 5720
- Pages
- 414-415
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2035571475
- Cited by
- 1,107
- References
- 0
- FWCI
- 21.23
- Type
- journal article
- Language
- en
- Open access
- closed
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- Nakajima, M., Imai, K., Ito, H., Nishiwaki, T., Murayama, Y., Iwasaki, H., Oyama, T., & Kondo, T. (2005). Reconstitution of Circadian Oscillation of Cyanobacterial KaiC Phosphorylation in Vitro. Science. https://doi.org/10.1126/science.1108451
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