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Chimeras of Two Isoprenoid Synthases Catalyze All Four Coupling Reactions in Isoprenoid Biosynthesis
Abstract
The carbon skeletons of over 55,000 naturally occurring isoprenoid compounds are constructed from four fundamental coupling reactions: chain elongation, cyclopropanation, branching, and cyclobutanation. Enzymes that catalyze chain elongation and cyclopropanation are well studied, whereas those that catalyze branching and cyclobutanation are unknown. We have catalyzed the four reactions with chimeric proteins generated by replacing segments of a chain-elongation enzyme with corresponding sequences from a cyclopropanation enzyme. Stereochemical and mechanistic considerations suggest that the four coupling enzymes could have evolved from a common ancestor through relatively small changes in the catalytic site.
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Cited by
Network Analysis of the MVA and MEP Pathways for Isoprenoid Synthesis
Vranová, E., Coman, D., & Gruissem, W. (2013) · Annual Review of Plant Biology · doi:10.1146/annurev-arplant-050312-120116 · Cited by 1,160
Terpene Biosynthesis: Modularity Rules
Oldfield, E., & Lin, F. (2012) · Angewandte Chemie International Edition · doi:10.1002/anie.201103110 · Cited by 391
Enzymatic Chemistry of Cyclopropane, Epoxide, and Aziridine Biosynthesis
Thibodeaux, C. J., Chang, W.-C., & Liu, H.-W. (2012) · Chemical Reviews · doi:10.1021/cr200073d · Cited by 275
Isopentenyl diphosphate isomerase: A checkpoint to isoprenoid biosynthesis
Berthelot, K., Estevez, Y., Deffieux, A., & Peruch, F. (2012) · Biochimie · doi:10.1016/j.biochi.2012.03.021 · Cited by 180
Metadata
- Authors
- Thulasiram, H. V., Erickson, H. K., & Poulter, C. D.
- Venue
- Science
- Published
- 2007-04-06
- Volume
- 316
- Issue
- 5821
- Pages
- 73-76
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W1990925961
- Cited by
- 191
- References
- 0
- FWCI
- 4.91
- Type
- journal article
- Language
- en
- Open access
- closed
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- Thulasiram, H. V., Erickson, H. K., & Poulter, C. D. (2007). Chimeras of Two Isoprenoid Synthases Catalyze All Four Coupling Reactions in Isoprenoid Biosynthesis. Science. https://doi.org/10.1126/science.1137786
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