The DOI matches its Crossref record; 14 of the 14 references it cites were found in a registry or an index.
The Phosphothreonine Lyase Activity of a Bacterial Type III Effector Family
Abstract
Pathogenic bacteria use the type III secretion system to deliver effector proteins into host cells to modulate the host signaling pathways. In this study, the Shigella type III effector OspF was shown to inactivate mitogen-activated protein kinases (MAPKs) [extracellular signal–regulated kinases 1 and 2 (Erk1/2), c-Jun N-terminal kinase, and p38]. OspF irreversibly removed phosphate groups from the phosphothreonine but not from the phosphotyrosine residue in the activation loop of MAPKs. Mass spectrometry revealed a mass loss of 98 daltons in p-Erk2, due to the abstraction of the α proton concomitant with cleavage of the C-OP bond in the phosphothreonine residue. This unexpected enzymatic activity, termed phosphothreonine lyase, appeared specific for MAPKs and was shared by other OspF family members.
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MAP Kinases in the Immune Response
Dong, C., Davis, R. J., & Flavell, R. A. (2002) · Annual Review of Immunology · doi:10.1146/annurev.immunol.20.091301.131133 · Cited by 1,662
Type III Secretion Machines: Bacterial Devices for Protein Delivery into Host Cells
Galán, J. E., & Collmer, A. (1999) · Science · doi:10.1126/science.284.5418.1322 · Cited by 1,319
Are innate immune signaling pathways in plants and animals conserved?
Ausubel, F. M. (2005) · Nature Immunology · doi:10.1038/ni1253 · Cited by 976
Yersinia YopJ Acetylates and Inhibits Kinase Activation by Blocking Phosphorylation
Mukherjee, S., Keitany, G., Li, Y., Wang, Y., Ball, H. L., Goldsmith, E. J., & Orth, K. (2006) · Science · doi:10.1126/science.1126867 · Cited by 615
CARD4/Nod1 mediates NF‐κB and JNK activation by invasive Shigella flexneri
Girardin, S. E., Tournebize, R., Mavris, M., Page, A., Li, X., Stark, G. R., Bertin, J., DiStefano, P. S., Yaniv, M., Sansonetti, P. J., et al. (2001) · EMBO reports · doi:10.1093/embo-reports/kve155 · Cited by 609
Cleavage of Arabidopsis PBS1 by a Bacterial Type III Effector
Shao, F., Golstein, C., Ade, J., Stoutemyer, M., Dixon, J. E., & Innes, R. W. (2003) · Science · doi:10.1126/science.1085671 · Cited by 558
Self-citationMacrophage Apoptosis by Anthrax Lethal Factor Through p38 MAP Kinase Inhibition
Park, J. M., Greten, F. R., Li, Z.-W., & Karin, M. (2002) · Science · doi:10.1126/science.1073163 · Cited by 500
Shao, F., Merritt, P. M., Bao, Z., Innes, R. W., & Dixon, J. E. (2002) · Cell · doi:10.1016/s0092-8674(02)00766-3 · Cited by 471
Self-citationThe Salmonella typhimurium virulence plasmid increases the growth rate of salmonellae in mice
Gulig, P. A., & Doyle, T. J. (1993) · Infection and Immunity · doi:10.1128/iai.61.2.504-511.1993 · Cited by 193
Innate immune responses of epithelial cells following infection with bacterial pathogens
Philpott, D. J., Girardin, S. E., & Sansonetti, P. J. (2001) · Current Opinion in Immunology · doi:10.1016/s0952-7915(00)00235-1 · Cited by 150
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Pitzschke, A., Schikora, A., & Hirt, H. (2009) · Current Opinion in Plant Biology · doi:10.1016/j.pbi.2009.06.008 · Cited by 710
Manipulation of host-cell pathways by bacterial pathogens
Bhavsar, A. P., Guttman, J. A., & Finlay, B. B. (2007) · Nature · doi:10.1038/nature06247 · Cited by 527
Alpha-kinase 1 is a cytosolic innate immune receptor for bacterial ADP-heptose
Zhou, P., She, Y., Dong, N., Li, P., He, H., Borio, A., Wu, Q., Lu, S., Ding, X., Cao, Y., et al. (2018) · Nature · doi:10.1038/s41586-018-0433-3 · Cited by 267
Self-citationThe Role of the spv Genes in Salmonella Pathogenesis
Guiney, D. G., & Fierer, J. (2011) · Frontiers in Microbiology · doi:10.3389/fmicb.2011.00129 · Cited by 223
Metadata
- Authors
- Li, H., Xu, H., Zhou, Y., Zhang, J., Long, C., Li, S., Chen, S., Zhou, J.-M., & Shao, F.
- Venue
- Science
- Published
- 2007-02-16
- Volume
- 315
- Issue
- 5814
- Pages
- 1000-1003
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2033098156
- Cited by
- 422
- References
- 14
- FWCI
- 16.65
- Type
- journal article
- Language
- en
- Open access
- closed
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