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Global Analysis of Cdk1 Substrate Phosphorylation Sites Provides Insights into Evolution
Open accessAbstract
Cataloging Kinase Targets: Protein phosphorylation is a central mechanism in the control of many biological processes (see the Perspective by Collins ). It remains a challenge to determine the complete range of substrates and phosphorylation sites altered by a kinase like cyclin-dependent kinase 1 (Cdk1), which controls cell division in yeast. Holt et al. (p. 1682 ) engineered a strain of yeast to express a modified Cdk1 molecule that could be inhibited by a specific small-molecule inhibitor. The range of Cdk1-dependent phosphorylation was assessed by quantitative mass spectrometry, which revealed many previously uncharacterized substrates for Cdk1. In addition to phosphorylation on serine and threonine residues, which appears to be evolutionarily ancient, tyrosine phosphorylation occurs primarily in multicellular organisms. Tan et al. (p. 1686 , published online 9 July) compared the overall presence of tyrosine residues in human proteins (which are frequently phosphorylated) and in yeast proteins (which are not). Loss of tyrosine residues has occurred during evolution, presumably to reduce adventitious tyrosine phosphorylation.
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Global analysis of protein expression in yeast
Ghaemmaghami, S., Huh, W.-K., Bower, K., Howson, R. W., Belle, A., Dephoure, N., O'Shea, E. K., & Weissman, J. S. (2003) · Nature · doi:10.1038/nature02046 · Cited by 3,750
The evolution of combinatorial gene regulation in fungi
Tuch, B. B., Galgoczy, D. J., Hernday, A. D., Li, H., & Johnson, A. D. (2008) · PLoS Biology · doi:10.1371/journal.pbio.0060038 · Cited by 228
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Metadata
- Authors
- Holt, L. J., Tuch, B. B., Villén, J., Johnson, A. D., Gygi, S. P., & Morgan, D. O.
- Venue
- Science
- Published
- 2009-09-25
- Volume
- 325
- Issue
- 5948
- Pages
- 1682-1686
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2085289028
- Cited by
- 869
- References
- 2
- FWCI
- 22.80
- Type
- journal article
- Language
- en
- Open access
- green
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