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Cited by 123Cellular transport and secretionNeuroscience and Neuropharmacology ResearchLipid Membrane Structure and Behavior

Dynamic Ca 2+ -Dependent Stimulation of Vesicle Fusion by Membrane-Anchored Synaptotagmin 1

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Lee, Han-Ki, Yang, Yoosoo, Su, Zengliu, Hyeon, Changbong, Lee, Tae-Sun, Lee, Hong-Won, Kweon, Dae-Hyuk, Shin, Yeon-Kyun +1 more·2010·Science
DOI 10.1126/science.1187722PMC PMC2994549
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Abstract

In neurons, synaptotagmin 1 (Syt1) is thought to mediate the fusion of synaptic vesicles with the plasma membrane when presynaptic Ca2+ levels rise. However, in vitro reconstitution experiments have failed to recapitulate key characteristics of Ca2+-triggered membrane fusion. Using an in vitro single-vesicle fusion assay, we found that membrane-anchored Syt1 enhanced Ca2+ sensitivity and fusion speed. This stimulatory activity of membrane-anchored Syt1 dropped as the Ca2+ level rose beyond physiological levels. Thus, Syt1 requires the membrane anchor to stimulate vesicle fusion at physiological Ca2+ levels and may function as a dynamic presynaptic Ca2+ sensor to control the probability of neurotransmitter release.

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  • Previous reconstitutions often employed lipid mixing, rather than content mixing, between vesicles with reconstituted neuronal SNAREs.

    “Previous reconstitutions often employed lipid mixing, rather than content mixing, between vesicles with reconstituted neuronal SNAREs21,83.”

    From Architecture of the synaptotagmin–SNARE machinery for neuronal exocytosis (2015) · cites this paper as [83]

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Authors
Lee, H.-K., Yang, Y., Su, Z., Hyeon, C., Lee, T.-S., Lee, H.-W., Kweon, D.-H., Shin, Y.-K., & Yoon, T.-Y.
Affiliations
  • Department of Physics, KAIST, Daejeon 305-701, South Korea.
  • Sungkyunkwan University (KR)
  • Iowa State University (US)
  • Korea Institute for Advanced Study (KR)
  • Korea Advanced Institute of Science and Technology (KR)
  • Pohang University of Science and Technology (KR)
Corresponding author
  • Yang, Yoosoo
  • Su, Zengliu
  • Shin, Yeon-Kyun
  • Yoon, Tae-Young
ORCID
Venue
Science
Publisher
American Association for the Advancement of Science (AAAS)
Published
2010-05-07
Volume
328
Issue
5979
Pages
760-763
ISSN
  • 0036-8075 (ISSN-L)
  • 1095-9203
DOI
10.1126/science.1187722
PMC
PMC2994549
OpenAlex
W1970237920
Cited by
123
References
1
Citations by year
2026: 2 · 2025: 1 · 2022: 4 · 2021: 2 · 2020: 4 · 2019: 12 · 2018: 5 · 2017: 7 · 2016: 5 · 2015: 13 · 2014: 12 · 2013: 16 · 2012: 14
FWCI
7.84
Type
journal article
Language
en
Open access
Locations
  • Science (publishedVersion)
  • PubMed (publishedVersion)
  • PubMed Central (acceptedVersion)
Funders
National Institute of General Medical Sciences (R01GM051290)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Lipid Membrane Structure and Behavior
Keywords
synaptotagmin-1, synaptic vesicle fusion, calcium sensitivity, neurotransmitter release probability, synaptic vesicle exocytosis
MeSH terms
Animals, Calcium, Magnesium, Membrane Fusion, Membrane Lipids, Synaptic Vesicles, Neurotransmitter Agents, Phosphatidylinositol 4,5-Diphosphate, SNARE Proteins, Synaptotagmin I, Rats

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