Targeting and plasticity of mitochondrial proteins revealed by proximity-specific ribosome profiling
Open accessAbstract
The wheres and whys of protein translation: Localized protein synthesis is important for a broad range of biological activities, from specifying the animal body plan to coordinating entry into the secretory pathway. Few tools are available that can investigate translation at specific subcellular sites. Jan et al. present a flexible ribosome profiling–based methodology to enable precise characterization of localized protein synthesis (see the Perspective by Shao and Hegde). Proximity-specific ribosome profiling provides a high-precision tool for looking at the mechanism of localized protein targeting and synthesis in living cells. The approach yielded a high-resolution systems-level view of cotranslational translocation at the endoplasmic reticulum. Williams et al. applied the technique to look at localized mRNA translation at the mitochondrial outer membrane. Science , this issue 10.1126/science.1257521 , p. 701 ; see also p. 748
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When examining published data on localized translation in yeast, we find that many yeast orthologs of the ATP synthase complex are translated near the mitochondria.
“and indeed, when examining published data on localized translation in yeast, we find that many yeast orthologs of the ATP synthase complex are translated near the mitochondria (Williams et al, 2014) (not shown).”
From Differential dynamics of the mammalian mRNA and protein expression response to misfolding stress (2016) · cites this paper as Williams et al, 2014
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Global analysis of protein localization in budding yeast
Huh, W.-K., Falvo, J. V., Gerke, L. C., Carroll, A. S., Howson, R. W., Weissman, J. S., & O'Shea, E. K. (2003) · Nature · doi:10.1038/nature02026 · Cited by 4,248 · see citing works
Self-citationThe Genetic Landscape of a Cell
Costanzo, M., Baryshnikova, A., Bellay, J., Kim, Y., Spear, E. D., Sevier, C. S., Ding, H., Koh, J. L. Y., Toufighi, K., Mostafavi, S., et al. (2010) · Science · doi:10.1126/science.1180823 · Cited by 2,162 · see citing works
Saccharomyces Genome Database: the genomics resource of budding yeast
Cherry, J., Hong, E., Amundsen, C., Balakrishnan, R., Binkley, G., Chan, E. T., Christie, K., Costanzo, M., Dwight, S., Engel, S., et al. (2011) · Nucleic Acids Res. · doi:10.1093/nar/gkr1029 · Cited by 1,886 · see citing works
Computational method to predict mitochondrially imported proteins and their targeting sequences.
Claros, M. G., & Vincens, P. (1996) · European Journal of Biochemistry · doi:10.1111/j.1432-1033.1996.00779.x · Cited by 1,736 · see citing works
An ER-Mitochondria Tethering Complex Revealed by a Synthetic Biology Screen
Kornmann, B., Currie, E., Collins, S. R., Schuldiner, M., Nunnari, J., Weissman, J. S., & Walter, P. (2009) · Science · doi:10.1126/science.1175088 · Cited by 1,275 · see citing works
Self-citationMitochondrial Import Efficiency of ATFS-1 Regulates Mitochondrial UPR Activation
Nargund, A. M., Pellegrino, M., Fiorese, C. J., Baker, B. M., & Haynes, C. M. (2012) · Science · doi:10.1126/science.1223560 · Cited by 1,027 · see citing works
Byrne, K. P., & Wolfe, K. H. (2005) · Genome Research · doi:10.1101/gr.3672305 · Cited by 720 · see citing works
Brandman, O., Stewart-Ornstein, J., Wong, D., Larson, A. G., Williams, C. C., Li, G., Zhou, S., King, D., Shen, P. S., Weibezahn, J., et al. (2012) · Cell · doi:10.1016/j.cell.2012.10.044 · Cited by 631 · see citing works
Self-citationDecoding Human Cytomegalovirus
Stern-Ginossar, N., Weisburd, B., Michalski, A., Le, V. T. K., Hein, M. Y., Huang, S.-X., Ma, M., Shen, B., Qian, S.-B., Hengel, H., et al. (2012) · Science · doi:10.1126/science.1227919 · Cited by 589 · see citing works
Self-citationGuarente, L., Lalonde, B. A., Gifford, P., & Alani, E. (1984) · Cell · doi:10.1016/0092-8674(84)90243-5 · Cited by 479 · see citing works
Signal Recognition Particle: An Essential Protein-Targeting Machine
Akopian, D., Shen, K., Zhang, X., & Shan, S. (2013) · Annual Review of Biochemistry · doi:10.1146/annurev-biochem-072711-164732 · Cited by 475 · see citing works
Tu, B. P., & Weissman, J. S. (2002) · Molecular Cell · doi:10.1016/s1097-2765(02)00696-2 · Cited by 433 · see citing works
Self-citationPrinciples of ER cotranslational translocation revealed by proximity-specific ribosome profiling
Jan, C. H., Williams, C. C., & Weissman, J. S. (2014) · Science · doi:10.1126/science.1257521 · Cited by 431 · see citing works
Self-citationGross, E., Sevier, C. S., Heldman, N., Vitu, E., Bentzur, M., Kaiser, C. A., Thorpe, C., & Fass, D. (2006) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0506448103 · Cited by 370 · see citing works
Resurrecting ancestral alcohol dehydrogenases from yeast
Thomson, J. M., Gaucher, E. A., Burgan, M. F., De Kee, D. W., Li, T., Aris, J. P., & Benner, S. A. (2005) · Nature Genetics · doi:10.1038/ng1553 · Cited by 341 · see citing works
Genome‐wide analysis of mRNAs targeted to yeast mitochondria
Marc, P., Margeot, A., Devaux, F., Blugeon, C., Corral‐Debrinski, M., & Jacq, C. (2002) · EMBO Reports · doi:10.1093/embo-reports/kvf025 · Cited by 317 · see citing works
Zielinska, D. F., Gnad, F., Schropp, K., et al. (2012) · Molecular Cell · doi:10.1016/j.molcel.2012.04.031 · Cited by 276 · see citing works
Kellems, R. E., Allison, V. F., & Butow, R. A. (1975) · The Journal of Cell Biology · doi:10.1083/jcb.65.1.1 · Cited by 270 · see citing works
Yeast Mitochondrial Biogenesis: A Role for the PUF RNA-Binding Protein Puf3p in mRNA Localization
Saint-Georges, Y., Garcia, M., Delaveau, T., Jourdren, L., Crom, S. L., Lemoine, S., Tanty, V., Devaux, F., & Jacq, C. (2008) · PLoS ONE · doi:10.1371/journal.pone.0002293 · Cited by 230 · see citing works
Mitochondrial Protein Synthesis, Import, and Assembly
Fox, T. D. (2012) · Genetics · doi:10.1534/genetics.112.141267 · Cited by 221 · see citing works
The conserved AAA-ATPase Msp1 confers organelle specificity to tail-anchored proteins
Okreglak, V., & Walter, P. (2014) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1405755111 · Cited by 217 · see citing works
Improved Blue, Green, and Red Fluorescent Protein Tagging Vectors for S. cerevisiae
Lee, S., Lim, W. A., & Thorn, K. S. (2013) · PLoS ONE · doi:10.1371/journal.pone.0067902 · Cited by 206 · see citing works
A Network of Cytosolic Factors Targets SRP-Independent Proteins to the Endoplasmic Reticulum
Ast, T., Cohen, G., & Schuldiner, M. (2013) · Cell · doi:10.1016/j.cell.2013.02.003 · Cited by 193 · see citing works
Robustness—it's not where you think it is
Wolfe, K. H. (2000) · Nature Genetics · doi:10.1038/75560 · Cited by 163 · see citing works
del Álamo, M., Hogan, D. J., Pechmann, S., Albanèse, V., Brown, P. O., & Frydman, J. (2011) · PLoS Biology · doi:10.1371/journal.pbio.1001100 · Cited by 158 · see citing works
Suissa, M., & Schatz, G. (1982) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)33620-2 · Cited by 133 · see citing works
Chapter 1 The Mitochondrial Proteome Database
Elstner, M., Andréoli, C., Klopstock, T., Meitinger, T., & Prokisch, H. (2009) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/s0076-6879(09)05001-0 · Cited by 85 · see citing works
Enomoto, K., Arikawa, Y., & Muratsubaki, H. (2002) · FEMS Microbiology Letters · doi:10.1111/j.1574-6968.2002.tb11377.x · Cited by 57 · see citing works
Camarasa, C., Faucet‐Marquis, V., & Dequin, S. (2007) · Yeast · doi:10.1002/yea.1467 · Cited by 41 · see citing works
One of the Fumarate Reductase Isoenzymes fromSaccharomyces cerevisiaeIs Encoded by theOSM1Gene
Muratsubaki, H., & Enomoto, K. (1998) · Archives of Biochemistry and Biophysics · doi:10.1006/abbi.1998.0583 · Cited by 32 · see citing works
Liu, Z., Österlund, T., Hou, J., et al. (2013) · Applied and Environmental Microbiology · doi:10.1128/aem.03207-12 · Cited by 26 · see citing works
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Weinberg, D. E., Shah, P., Eichhorn, S. W., Hussmann, J. A., Plotkin, J. B., & Bartel, D. P. (2016) · Cell Reports · doi:10.1016/j.celrep.2016.01.043 · Cited by 392 · see citing works
Organization and regulation of mitochondrial protein synthesis
Ott, M., Amunts, A., & Brown, A. (2016) · Annual Review of Biochemistry · doi:10.1146/annurev-biochem-060815-014334 · Cited by 282 · see citing works
Differential dynamics of the mammalian mRNA and protein expression response to misfolding stress
Cheng, Z., Teo, G., Krueger, S., Rock, T. M., Koh, H. W., Choi, H., & Vogel, C. (2016) · Molecular Systems Biology · doi:10.15252/msb.20156423 · Cited by 227 · see citing works
Mapping spatial transcriptome with light-activated proximity-dependent RNA labeling
Wang, P., Tang, W., Li, Z., Zou, Z., Zhou, Y., Li, R., Xiong, T., Wang, J., & Zou, P. (2019) · Nature Chemical Biology · doi:10.1038/s41589-019-0368-5 · Cited by 150 · see citing works
Mitochondrial stress-dependent regulation of cellular protein synthesis
Topf, U., Uszczynska-Ratajczak, B., & Chacinska, A. (2019) · Journal of Cell Science · doi:10.1242/jcs.226258 · Cited by 52 · see citing works
- Authors
- Williams, C. C., Jan, C. H., & Weissman, J. S.
- Affiliations
- These authors contributed equally to this work.
- QB3 (US)
- Corresponding author
- Weissman, Jonathan S.
- Venue
- Science
- Published
- 2014-11-07
- Volume
- 346
- Issue
- 6210
- Pages
- 748-751
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- PMC
- PMC4263316
- OpenAlex
- W2057015366
- Cited by
- 391
- References
- 31
- Citations by year
- 2026: 17 · 2025: 30 · 2024: 37 · 2023: 44 · 2022: 29 · 2021: 40 · 2020: 33 · 2019: 39 · 2018: 29 · 2017: 43 · 2016: 22 · 2015: 21 · 2014: 5 · 2013: 1
- FWCI
- 12.43
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Science (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Funders
- Howard Hughes Medical Institute
- National Institute of General Medical Sciences (P50GM102706)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mitochondrial Function and Pathology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Biotin and Related Studies
- Keywords
- mitochondrial protein targeting, organelle targeting, nuclear-encoded mitochondrial proteins, inner membrane proteins, endoplasmic reticulum localization, alternative translation initiation sites, oxidative protein folding, mitochondrial translation, Saccharomyces cerevisiae
- MeSH terms
- Animals, Endoplasmic Reticulum, Mitochondria, Peptide Chain Initiation, Translational, Ribosomes, Saccharomyces cerevisiae, Succinate Dehydrogenase, Protein Folding, Mitochondrial Proteins, Saccharomyces cerevisiae Proteins
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