Phylogenomics resolves the timing and pattern of insect evolution
Abstract
Toward an insect evolution resolution: Insects are the most diverse group of animals, with the largest number of species. However, many of the evolutionary relationships between insect species have been controversial and difficult to resolve. Misof et al. performed a phylogenomic analysis of protein-coding genes from all major insect orders and close relatives, resolving the placement of taxa. The authors used this resolved phylogenetic tree together with fossil analysis to date the origin of insects to ~479 million years ago and to resolve long-controversial subjects in insect phylogeny. Science , this issue p. 763
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The diversification of angiosperms since the Cretaceous coincides with the extraordinarily rapid diversification within clades of holometabolous insects (primarily bees, flies and butterflies).
“The diversification of angiosperms since the Cretaceous [48–50] coincides with the extraordinarily rapid diversification within clades of holometabolous insects (primarily bees, flies and butterflies) [51].”
From Novelty and Innovation in the History of Life (2015) · cites this paper as [51]
Complete metamorphosis (holometaboly) in insects evolved once, approximately 344 million years ago (95% confidence interval 372 to 317 Ma).
“Complete metamorphosis (holometaboly) in insects evolved once, some 344 million years ago (95% confidence interval: 372 to 317) (Misof et al. , 2014).”
From Why grow up? <scp>A</scp> perspective on insect strategies to avoid metamorphosis (2016) · cites this paper as Misof et al., 2014
Hemimetabolous development appeared after the evolution of wings.
“The first type of metamorphic development (hemimetabolous development) appeared after the evolution of wings [42].”
From Regulation of metamorphosis in holometabolous insects (2026) · cites this paper as [42]
All the supermatrices had also been previously extensively analyzed using either RAxML or ExaML.
“In addition to our analyses, all the supermatrices have also been previously extensively analyzed using either RAxML or ExaML (e.g., Jarvis et al. 2014; Misof et al. 2014; Wickett et al. 2014).”
From Evaluating fast maximum likelihood-based phylogenetic programs using empirical phylogenomic data sets (2018) · cites this paper as Misof et al. 2014
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Inferring phylogenies with incomplete data sets: a 5-gene, 567-taxon analysis of angiosperms
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Scratching an ancient itch: an Eocene bird louse fossil
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On the phylogeny and evolution of Mesozoic and extant lineages of Adephaga (Coleoptera, Insecta)
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SOME REMARKS ON THE DEVONIAN FOSSIL INSECTS FROM THE RHYNIE CHERT BEDS, OLD RED SANDSTONE
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Head morphology of Tricholepidion gertschi indicates monophyletic Zygentoma
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Self-citationThe Earliest Webspinners (Insecta: Embiodea)
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Diversity Dynamics of Silurian–Early Carboniferous Land Plants in South China
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Sperm accessory microtubules suggest the placement of Diplura as the sister-group of Insecta s.s.
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A New Species of Zorotypus from Central Amazonia, Brazil (Zoraptera: Zorotypidae)
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Miocene honey bees from the Randeck Maar of southwestern Germany (Hymenoptera, Apidae)
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Self-citationA New Japygid Dipluran from the Lower Cretaceous of Brazil
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Depositional environments, palynology, and age of the Dakota Formation, south-central Utah
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The Relict Scorpionfly Family Meropeidae (Mecoptera) in Cretaceous Amber
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The External Anatomy of the Black Horse-Fly Tabanus Atratus Fab. (Diptera: Tabanidæ).*
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A New Species of Emballonura (Chiroptera: Emballonuridae) from the Dry Regions of Madagascar
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Some new Permian Insects from Belmont, N.S.W., in the collection of Mr. John Mitchell
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A fossil tsetse fly and other Diptera from Florissant, Colorado
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Self-citationLaflamme (2004) · doi:10.1666/0022-3360(2004)078
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- Authors
- Misof, B., Liu, S., Meusemann, K., Peters, R. S., Donath, A., Mayer, C., Frandsen, P. B., Ware, J., Flouri, T., Beutel, R. G., Niehuis, O., Petersen, M., Izquierdo-Carrasco, F., Wappler, T., Rust, J., Aberer, A. J., Aspöck, U., Aspöck, H., Bartel, D., Blanke, A., Berger, S., Böhm, A., Buckley, T. R., Calcott, B., Chen, J., Friedrich, F., Fukui, M., Fujita, M., Greve, C., Grobe, P., Gu, S., Huang, Y., Jermiin, L. S., Kawahara, A. Y., Krogmann, L., Kubiak, M., Lanfear, R., Letsch, H., Li, Y., Li, Z., Li, J., Lu, H., Machida, R., Mashimo, Y., Kapli, P., McKenna, D. D., Meng, G., Nakagaki, Y., Navarrete-Heredia, J. L., Ott, M., Ou, Y., Pass, G., Podsiadlowski, L., Pohl, H., von Reumont, B. M., Schütte, K., Sekiya, K., Shimizu, S., Slipinski, A., Stamatakis, A., Song, W., Su, X., Szucsich, N. U., Tan, M., Tan, X., Tang, M., Tang, J., Timelthaler, G., Tomizuka, S., Trautwein, M., Tong, X., Uchifune, T., Walzl, M. G., Wiegmann, B. M., Wilbrandt, J., Wipfler, B., Wong, T. K. F., Wu, Q., Wu, G., Xie, Y., Yang, S., Yang, Q., Yeates, D. K., Yoshizawa, K., Zhang, Q., Zhang, R., Zhang, W., Zhang, Y., Zhao, J., Zhou, C., Zhou, L., Ziesmann, T., Zou, S., Li, Y., Xu, X., Zhang, Y., Yang, H., Wang, J., Wang, J., Kjer, K. M., & Zhou, X.
- Affiliations
- Zoological Research Museum Alexander Koenig (DE)
- BGI Group (China) (CN)
- Commonwealth Scientific and Industrial Research Organisation (AU)
- Rutgers, The State University of New Jersey (US)
- Heidelberg Institute for Theoretical Studies (DE)
- Friedrich Schiller University Jena (DE)
- University of Bonn (DE)
- Natural History Museum Vienna (AT)
- Medical University of Vienna (AT)
- University of Vienna (AT)
- University of Tsukuba (JP)
- Manaaki Whenua – Landcare Research (NZ)
- Johns Hopkins University (US)
- Universität Hamburg (DE)
- Ehime University (JP)
- Florida Museum of Natural History (US)
- Staatliches Museum für Naturkunde Stuttgart (DE)
- Australian National University (AU)
- University of Crete (GR)
- University of Memphis (US)
- Centro Universitario de Ciencias Biólogicas y Agropecuarias, Centro de Estudios en Zoología, Universidad de Guadalajara, Zapopan, Jalisco, México.
- Bavarian Academy of Sciences and Humanities (DE)
- Institute of Evolutionary Biology and Ecology, Zoology and Evolutionary Biology, University of Bonn, Bonn, Germany.
- Department of Life Sciences, The Natural History Museum London, London, UK.
- Australian National Insect Collection, Commonwealth Scientific and Industrial Research Organization (Australia) (CSIRO), National Research Collections Australia, Canberra, ACT, Australia.
- Karlsruhe Institute of Technology (DE)
- California Academy of Sciences (US)
- South China Agricultural University (CN)
- North Carolina State University (US)
- Institut für Spezielle Zoologie und Evolutionsbiologie mit Phyletischem Museum Jena, FSU Jena, Germany.
- Hokkaido University (JP)
- ORCID
- Misof, Bernhard
- Liu, Shanlin
- Meusemann, Karen
- Peters, Ralph S.
- Donath, Alexander
- Mayer, Christoph
- Frandsen, Paul B.
- Ware, Jessica
- Flouri, Tomáš
- Beutel, Rolf G.
- Niehuis, Oliver
- Petersen, Malte
- Wappler, Torsten
- Rust, Jes
- Aspöck, Ulrike
- Aspöck, Horst
- Blanke, Alexander
- Böhm, Alexander
- Buckley, Thomas R.
- Calcott, Brett
- Chen, Junqing
- Friedrich, Frank
- Fukui, Makiko
- Greve, Carola
- Grobe, Peter
- Huang, Ying
- Jermiin, Lars S.
- Kawahara, Akito Y.
- Krogmann, Lars
- Lanfear, Robert
- Letsch, Harald
- Li, Yiyuan
- Li, Zhenyu
- Li, Jiguang
- Lu, Haorong
- Machida, Ryuichiro
- Mashimo, Yuta
- McKenna, Duane D.
- Meng, Guanliang
- Pohl, Hans
- Shimizu, Shota
- Stamatakis, Alexandros
- Su, Xu
- Szucsich, Nikolaus U.
- Tan, Xuemei
- Tang, Min
- Tang, Jingbo
- Timelthaler, Gerald
- Tomizuka, Shigekazu
- Trautwein, Michelle
- Tong, Xiaoli
- Wiegmann, Brian M.
- Wilbrandt, Jeanne
- Wong, Thomas K. F.
- Wu, Qiong
- Yang, Qing
- Yeates, David K.
- Yoshizawa, Kazunori
- Zhang, Qing
- Zhang, Wenwei
- Zhang, Yunhui
- Zhao, Jing
- Zhou, Chengran
- Zhou, Lili
- Ziesmann, Tanja
- Li, Yingrui
- Xu, Xun
- Zhang, Yong
- Yang, Huanming
- Wang, Jian
- Wang, Jun
- Kjer, Karl M.
- Zhou, Xin
- Venue
- Science
- Published
- 2014-11-07
- Volume
- 346
- Issue
- 6210
- Pages
- 763-767
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2059359550
- Cited by
- 2,694
- References
- 170
- Citations by year
- 2026: 156 · 2025: 185 · 2024: 218 · 2023: 203 · 2022: 274 · 2021: 253 · 2020: 270 · 2019: 249 · 2018: 247 · 2017: 225 · 2016: 246 · 2015: 161 · 2014: 5
- FWCI
- 217.97
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Science (publishedVersion)
- PubMed (publishedVersion)
- Research at the University of Copenhagen (University of Copenhagen) (publishedVersion)
- DESY Publication Database (PUBDB) (Deutsches Elektronen-Synchrotron) (submittedVersion)
- DORA PSI (Paul Scherrer Institute) (submittedVersion)
- UCL Discovery (University College London) (submittedVersion)
- ANU Open Research (Australian National University) (submittedVersion)
- Funders
- Japan Society for the Promotion of Science (14F04071, 25440201, 24570093)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Ecology, Evolution, Behavior and Systematics › Fossil Insects in Amber
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Phylogenetic Studies
- Life Sciences › Agricultural and Biological Sciences › Ecology, Evolution, Behavior and Systematics › Coleoptera Taxonomy and Distribution
- Keywords
- insect phylogeny, insect evolution, insect origin, holometabolous insects, diversification timing, protein-coding genes, divergence time estimation, Ordovician, Devonian, Mississippian, Cretaceous, evolutionary innovation
- MeSH terms
- Animals, Genetic Code, Insecta, Phylogeny, Time Factors, Insect Proteins, Genomics, Genome, Insect
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
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