Crystal structures of translocator protein (TSPO) and mutant mimic of a human polymorphism
Open accessAbstract
Structural clues to protein function: Translocator protein (TSPO) is a mitochondrial membrane protein thought to transport cholesterol and porphyrins. Its detailed function remains unclear, but interest in it is high because TSPO is involved in a variety of human diseases. Two papers now present crystal structures of bacterial TSPOs. Li et al.show that a mutant that mimics a human single polymorphism associated with psychiatric disorders has structural changes in a region implicated in cholesterol binding. Guo et al.suggest that TSPO may be more than a transporter. They show how it catalyzes the degradation of porphyrins, a function that could be important in protection against oxidative stress. Science, this issue p.555, p.551
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TSPO is predominantly α-helical, containing five helices clustered into a barrel-like shape.
“Biochemical evidence and recent structural determinations of the TSPO protein indicate that TSPO is predominantly α-helical, containing five helices clustered into a barrel-like shape (113–115).”
From Adrenal Mitochondria and Steroidogenesis: From Individual Proteins to Functional Protein Assemblies (2016) · cites this paper as [114]
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Cites
Enigma of the peripheral benzodiazepine receptor
Gavish, M., Bachman, I., Shoukrun, R., Katz, Y., Veenman, L., Weisinger, G., & Weizman, A. (1999) · Pharmacological Reviews · doi:10.1016/s0031-6997(24)01424-8 · Cited by 512 · see citing works
Lacapère, J.-J., & Papadopoulos, V. (2003) · Steroids · doi:10.1016/s0039-128x(03)00101-6 · Cited by 342 · see citing works
Ji, B., Maeda, J., Sawada, M., Ono, M., Okauchi, T., Inaji, M., Zhang, M.-R., Suzuki, K., Ando, K., Staufenbiel, M., et al. (2008) · The Journal of Neuroscience · doi:10.1523/jneurosci.2312-08.2008 · Cited by 169 · see citing works
The origin of mitochondria in light of a fluid prokaryotic chromosome model
Esser, C., Martin, W., & Dagan, T. (2007) · Biology Letters · doi:10.1098/rsbl.2006.0582 · Cited by 92 · see citing works
Costa, B., Pini, S., Gabelloni, P., Da Pozzo, E., Abelli, M., Lari, L., Preve, M., Lucacchini, A., Cassano, G. B., & Martini, C. (2009) · Endocrinology · doi:10.1210/en.2009-0752 · Cited by 68 · see citing works
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Research works printed but not linked: 30
- [1]Fan J Papadopoulos V PLOS ONE 8 e76701 2013 24124589 PMC3790746
- [2]Fan J Lindemann P Feuilloley MG Papadopoulos V Curr Mol Med 12 369 386 2012 22364126
- [3]Bird JL Atherosclerosis 210 388 391 2010 20056222 PMC2884178
- [4]Hardwick M Cancer Res 59 831 842 1999 10029072
- [5]Ji B J Neurosci 28 12255 12267 2008 19020019 PMC2755188
- [7]Costa B Endocrinology 150 5438 5445 2009 19846611
- [8]Owen DR J Cereb Blood Flow Metab 32 1 5 2012 22008728 PMC3323305
- [9]Colasanti A Psychoneuroendocrinology 38 2826 2829 2013 23942012 PMC3820042
- [10]Nakamura K Am J Med Genet B Neuropsychiatr Genet 141B 222 226 2006 16511838
- [11]Costa B Psychiatr Genet 19 110 111 2009 19668118
- [12]Kreisl WC J Cereb Blood Flow Metab 33 53 58 2013 22968319 PMC3597372
- [13]Yeliseev AA Kaplan S J Biol Chem 275 5657 5667 2000 10681549
- [14]Li F Xia Y Meiler J Ferguson-Miller S Biochemistry 52 5884 5899 2013 23952237 PMC3756528
- [15]Korkhov VM Sachse C Short JM Tate CG Structure 18 677 687 2010 20541505 PMC2911597
- [16]Zeno S Curr Mol Med 12 494 501 2012 22376065
- [17]Yeliseev AA Krueger KE Kaplan S Proc Natl Acad Sci USA 94 5101 5106 1997 9144197 PMC24638
- [18]Yeliseev AA Kaplan S J Biol Chem 274 21234 21243 1999 10409680
- [19]Esser C Martin W Dagan T Biol Lett 3 180 184 2007 17251118 PMC2375920
- [20]Li H Papadopoulos V Endocrinology 139 4991 4997 1998 9832438
- [21]Moore DT Berger BW DeGrado WF Structure 16 991 1001 2008 18611372 PMC3771515
- [22]Jaremko L Jaremko M Giller K Becker S Zweckstetter M Science 343 1363 1366 2014 24653034 PMC5650047
- [23]Farges R Mol Pharmacol 46 1160 1167 1994 7808437
- [25]Qin L Hiser C Mulichak A Garavito RM Ferguson-Miller S Proc Natl Acad Sci USA 103 16117 16122 2006 17050688 PMC1616942
- [26]Dutzler R Schirmer T Karplus M Fischer S Structure 10 1273 1284 2002 12220498
- [27]Delavoie F Biochemistry 42 4506 4519 2003 12693947
- [28]Lacapère JJ Papadopoulos V Steroids 68 569 585 2003 12957662
- [29]Gatliff J Campanella M Curr Mol Med 12 356 368 2012 22364127
- [30]Scarf AM Curr Mol Med 12 488 493 2012 22348617
- [31]Rupprecht R Nat Rev Drug Discov 9 971 988 2010 21119734
- [32]Morohaku K Endocrinology 155 89 97 2014 24174323 PMC3868810
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Gault, J., Liko, I., Landreh, M., Shutin, D., Bolla, J. R., Jefferies, D., Agasid, M., Yen, H.-Y., Ladds, M. J. G. W., Lane, D. P., et al. (2020) · Nature Methods · doi:10.1038/s41592-020-0821-0 · Cited by 171 · see citing works
Steroid biosynthesis in adipose tissue
Li, J., Papadopoulos, V., & Vihma, V. (2015) · Steroids · doi:10.1016/j.steroids.2015.03.016 · Cited by 130 · see citing works
Fan, J., Campioli, E., Midzak, A., Culty, M., & Papadopoulos, V. (2015) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1502670112 · Cited by 133 · see citing works
Adrenal Mitochondria and Steroidogenesis: From Individual Proteins to Functional Protein Assemblies
Midzak, A., & Papadopoulos, V. (2016) · Frontiers in Endocrinology · doi:10.3389/fendo.2016.00106 · Cited by 114 · see citing works
Midzak, A. S., Akula, N., Rone, M. B., & Papadopoulos, V. (2015) · Pharmacological Research · doi:10.1016/j.phrs.2015.03.023 · Cited by 20 · see citing works
- Authors
- Li, F., Liu, J., Zheng, Y., Garavito, R. M., & Ferguson-Miller, S.
- Affiliations
- Michigan State University (US)
- Corresponding author
- Ferguson-Miller, Shelagh
- Venue
- Science
- Published
- 2015-01-30
- Volume
- 347
- Issue
- 6221
- Pages
- 555-558
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- PMC
- PMC5125025
- OpenAlex
- W2069121275
- Cited by
- 163
- References
- 5
- Citations by year
- 2026: 3 · 2025: 9 · 2024: 5 · 2023: 5 · 2022: 11 · 2021: 16 · 2020: 27 · 2019: 15 · 2018: 11 · 2017: 16 · 2016: 13 · 2015: 32
- FWCI
- 9.88
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Science (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- OSTI OAI (U.S. Department of Energy Office of Scientific and Technical Information) (submittedVersion)
- Funders
- NIH (GM26916)
- National Cancer Institute (ACB-12002)
- National Institute of General Medical Sciences (AGM-12006)
- Michigan State University Strategic Partnership Grant
- Mitochondrial Science and Medicine
- Topics
- Physical Sciences › Materials Science › Materials Chemistry › Enzyme Structure and Function
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Lipid Membrane Structure and Behavior
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Receptor Mechanisms and Signaling
- Keywords
- translocator protein (TSPO), Rhodobacter sphaeroides, cholesterol binding, porphyrin ligands, mental illness, lipidic cubic phase
- MeSH terms
- Amino Acid Sequence, Bacterial Proteins, Binding Sites, Cholesterol, Humans, Hydrogen Bonding, Isoquinolines, Ligands, Models, Molecular, Molecular Sequence Data, Porphyrins, Protein Conformation, Protoporphyrins, Rhodobacter sphaeroides, Protein Structure, Secondary, Receptors, GABA, Crystallography, X-Ray, Polymorphism, Single Nucleotide, Membrane Transport Proteins, Mutant Proteins, Protein Multimerization
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